• Plant Resources
• /
• v.6 no.1
• /
• pp.16-26
• /
• 2003

Molecular markers are useful tools for evaluating genetic diversity and determining cultivar identity. Present study was conducted to evaluate the genetic diversity within a diverse collection of rice accessions used for Korean breeding programs. Two hundred eighty-seven rice cultivars, composed of temperate japonica, tropical japonica, indica, and Tongil-type of Korean crossing parents were evaluated by means of 15 simple sequence repeat (SSR) markers. A total of 99 alleles were detected, and the number of alleles per marker ranged from 4 to 11, with an average of 6.6 per locus. Polymorphism information content (PIC) for each of the SSR markers ranged from 0.2924 to 0.8102 with an average of 0.5785. These results, with the result that use of only 15 SSR markers made all rice cultivars examined could be uniquely distinguished, imply the efficiency of SSR markers for analysis of genetic diversity in rice. Cluster analysis was performed on similar coefficient matrics calculated from SSR markers to generate a dendogram in which two major groups corresponding to japonica (Group I) and indica and Tongil type rice (group II) with additional subclasses within both major groups. The narrowness of the Korean breeding germplasm was revealed by the fact that most of the Korean-bred and Japan-bred temperate japonica cultivars were concentrated into only 2 of the sub-group I-1 (143 cultivars) and I-2 (58 cultivars) among six sub-groups in major group of japonica. This is because of the japonica accessions used in this study was a very closely related ones because of frequent sharing of the crossing parents with similar genetic background with synergy effect of the inherited genetic difference between indica and japonica. A rice breeding strategy with the use of molecular markers was discussed for overcoming of genetic vulnerability owing to this genetic narrowness.

• Proceedings of the Plant Resources Society of Korea Conference
• /
• 2018.10a
• /
• pp.100-100
• /
• 2018

Zoysia japonica Steud.(Zj) is a typical warm-season Korean lawn grass, which is used in many places such as river banks, roadside and soccer fields in Korea. Recently, it has also been used in school yards and the Saemangeum reclaimed land to reduce water pollution. Although the cultivated area of turfgrass is steadily increasing worldwide, it grows fast requiring frequent mowing and is difficult to grow in shady areas and the cold region. Therefore this study aims searching for useful gene(s) to develop abiotic stress tolerant and dwarf zoysiagrass. We isolated Ycf4 gene based on the sequence from Oryza sativa Japonica through RT-PCR and RACE PCR. Ultimately, open reading frame (ORF) of ZjYcf4 was 558bp long, encoding a protein of 186 amino acid residues. NCBI blast results showed that the ZjYcf4 protein is evolutionarily closely related to Ycf4 protein from Zoysia macrantha and Setaria italica (100% and 98%, respectively). To determine whether ZjYcf4 was involved in environmental stress in wild-type zoysiagrass, expression patterns of the gene were analyzed by real-time PCR under salt, cold and dark conditions. They were analyzed after each stress treatment for 3 hours. In salt and cold stresses, the expression was higher compared to control (3-fold and 1.5-fold, respectively), although there was a 1.6-fold decrease in expression under dark stress treatment. As reported previously, we suggest that ZjYcf4 gene affects abiotic stress such as salt, cold and dark.

• Proceedings of the Plant Resources Society of Korea Conference
• /
• 2018.10a
• /
• pp.47-47
• /
• 2018

붉가시나무는 기후변화에 따라 분포 확산이 예측되고 목재로서의 이용가치가 증가하고 있다. 본 연구는 국내에서 붉가시나무의 자원량 및 생육환경을 파악하기 위하여 국내 분포 및 자생지의 식생구조를 분석하였다. 분포 범위 조사 결과, 제주도를 비롯한 남해안의 도서지역에 주로 분포하는데, 제주도 남부지역 약 200~800m에 분포하며 가거도, 완도, 진도 등에 주로 분포하였다. 지역별 자생지의 입지분석 결과, 교목층의 수고는 제주도에서 17.3m로 가장 높고 가거도의 경우 11.3m로 가장 낮았다. 또한 가거도 자생지의 경우 노출암 비율 70.7%, 평균 경사도 약 $30^{\circ}$로 경사가 매우 급하고 토양층이 가장 빈약하였다. 반면, 제주도에서는 계곡 사면이나 완만한 경사의 산록지대에 주로 분포하였다. 자생지의 식생구조를 파악하기 위하여 수종별 평균중요도지수를 산출한 결과, 교목층에서는 붉가시나무가 가장 높았다. 아교목층의 평균중요도지수는 제주도, 완도, 진도 자생지의 경우 동백나무가 각각 150.93, 83.54, 91.38로 가장 높았는데 이는 동백나무의 밀도가 상대적으로 높았기 때문이다. 반면 가거도 자생지에서는 붉가시나무의 평균중요도지수가 66.55로 가장 높게 산출되었다. 자생지 내 수종의 흉고직경에 따른 수고의 분포는 제주도, 진도, 완도 자생지의 경우 붉가시나무와 기타 수종이 전체 층위에서 고르게 분포하였지만 가거도 자생지에서는 상층일수록 경쟁 종없이 단일종이 분포하는 단순림으로 나타났다. 이 같은 단순림에서는 일시적인 교란에 의해 상층 개체가 없어지면 안정적으로 후대 개체를 생산할 수 없기 때문에 숲 가꾸기와 같은 적극적인 조절이 필요한 것으로 판단된다. 종다양도지수 분석결과, 층위가 높을수록 균재도가 낮게 나타났다. 이와 같은 연구결과는 붉가시나무림의 조성이나 안정적 유지 등 인위적 조절을 위한 기초 자료로 활용 할 수 있을 것으로 판단된다.

• Journal of the Korean Association of Geographic Information Studies
• /
• v.19 no.1
• /
• pp.94-105
• /
• 2016

This study was carried out to assess the distributional probability for Evergreen Broad-Leaved Forests(EBLFs) using the field data and digital climate data that were occurred during the period of 1980 to 2010. For the validation of logistic regression model, the probabilistic value ranged from 33 to 84%, especially the probabilistic value of growing distribution becomes lower patterns with higher altitude. In addition, it has been estimated that the probabilistic value of growing distribution is the highest with 63~83% among the regional units in temperate/warm-temperate forests.

• The Korean Journal of Malacology
• /
• v.28 no.3
• /
• pp.277-291
• /
• 2012

Aquaculture is challenged by a number of constraints with future efforts towards sustainable production. Global climate change has a potential damage to the sustainability by changing environmental surroundings unfavorably. The damaging parameters identified are water temperature, sea level, surface physical energy, precipitation, solar radiation, ocean acidification, and so on. Of them, temperature, mostly temperature elevation, occupies significant concern among marine ecologists and aquaculturists. Ocean acidification particularly draws shellfish aquaculturists' attention as it alters the marine chemistry, shifting the equilibrium towards more dissolved CO2 and hydrogen ions ($H^+$) and thus influencing signaling pathways on shell formation, immune system, and other biological processes. Temperature elevation by climate change is of double-sidedness: it can be an opportunistic parameter besides being a generally known damaging parameter in aquaculture. It can provide better environments for faster and longer growth for aquaculture species. It is also somehow advantageous for alleviation of aquaculture expansion pressure in a given location by opening a gate for new species and aquaculture zone expansion northward in the northern hemisphere, otherwise unavailable due to temperature limit. But in the science of climate change, the ways of influence on aquaculture are complex and ambiguous, and hence are still hard to identify and quantify. At the same time considerable parts of our knowledge on climate change effects on aquaculture are from the estimates from data of fisheries and agriculture. The consequences may be different from what they really are, particularly in the temperature region. In reality, bivalves and tunicates hung or caged in the longline system are often exposed to temperatures higher than those they encounter in nature, locally driving the farmed shellfish into an upper tolerable temperature extreme. We review recent climate change and following environment changes which can be factors or potential factors affecting shellfish aquaculture production in the temperate region.

• The Korean Journal of Ecology
• /
• v.4 no.1_2
• /
• pp.52-57
• /
• 1981

I have studied serveral patterns of flowering periods in selected floras of the world since 1969. The total sums and patterns of flowering periods in Netherland, South Germany, France, and Mediterranean and Palestine floras are compared. The total sums of flowering periods of the floras are gradually increased from Netherland to Palestine. The patterns of these flowering periods are characteristic, and divided into two categories; northern temperate and Mediteranean climatic types. The patterns of Netherland, south Germany, and France flora belong to the northern temperate climatic type, and thus the peak of flowering period occur in the month of July. These patterns are particularly belong to the Meditteranean climatic type; and thus the peaks of patterns of flowering period occur in April or May. The Increasing patterns of flowering period from January to April or May seems to be dependent upon the combined factors of satisfactory precipitatiion an dincreasing temperature, while the decreasing patterns since the month of the peak is presumably greatly affected by the scanty precipitation in the high temperature seasons.

• The Korean Journal of Ecology
• /
• v.17 no.1
• /
• pp.37-47
• /
• 1994

A study was conducted to examine the leaf expansion forms and to analyze the leaf growth in early growing season of 1992 in a temperate deciduous forest in central region of Korea. After the winter bud scale fell off, the expansion forms of 11 woody species were divided into 3 groups, spreading fan form, opening form from half folding, and unrolling form from main vein. The ratios of leaf area at the end of growing season to that of leaf expantion time varied among species, and were related closely to expansion forms. The leaves reached to full size between the third ten days of April and the middle ten days of May, except for a few species. Leaf weight, however, increased steadily during the growing season. Specific leaf area (SLA) increased rapidly for 10-20 days after leaf expansion and decreased rapidly for 10 days after reaching maximum values, and thereafter decreased slowly. The SLA values of trees were smaller than $200cm^2/g$, but those of subtree and shrub were larger than $200cm^2/g$.

• Journal of Ecology and Environment
• /
• v.33 no.2
• /
• pp.165-173
• /
• 2010

Soil respiration ($R_S$) is a critical component of the annual carbon balance of forests, but few studies thus far have attempted to evaluate empirical regression models in $R_S$. The principal objectives of this study were to evaluate the relationship between $R_S$ rates and soil temperature (ST) and soil water content (SWC) in soil from a cool-temperate deciduous broad-leaved forest, and to evaluate empirical regression models for the prediction of $R_S$ using ST and SWC. We have been measuring $R_S$, using an open-flow gas-exchange system with an infrared gas analyzer during the snowfree season from 1999 to 2001 at the Takayama Forest, Japan. To evaluate the empirical regression models used for the prediction of $R_S$, we compared a simple exponential regression (flux = $ae^{bt}$Eq. [1]) and two polynomial multiple-regression models (flux = $ae^{bt}{\times}({\theta}{\nu}-c){\times}(d-{\theta}{\nu})^f:$ Eq. [2] and flux = $ae^{bt}{\times}(1-(1-({\theta}{\nu}/c))^2)$: Eq. [3]) that included two variables (ST: t and SWC: ${\theta}{\nu}$) and that utilized hourly data for $R_S$. In general, daily mean $R_S$ rates were positively well-correlated with ST, but no significant correlations were observed with any significant frequency between the ST and $R_S$ rates on periods of a day based on the hourly $R_S$ data. Eq. (2) has many more site-specific parameters than Eq. (3) and resulted in some significant underestimation. The empirical regression, Eq. (3) was best explained by temporal variations, as it provided a more unbiased fit to the data compared to Eq. (2). The Eq. (3) (ST $\times$ SWC function) also increased the predictive ability as compared to Eq. (1) (only ST exponential function), increasing the $R^2$ from 0.71 to 0.78.

• Journal of Environmental Science International
• /
• v.18 no.11
• /
• pp.1309-1324
• /
• 2009

This study was survey a flora and life form spectrum of 11 uninhabited islets, in jeju-do. The vascular plants of each uninhabited islets were summarized as Beomseom 159 taxa, Chagwi-do 136 taxa, Daryeo-do 81 taxa, Hyeongje-do 61 taxa, Jigwi-do 39 taxa, Munseom 147 taxa, Ran-do 58 taxa, Saeseom 65 taxa, Seogeondo 60 taxa, Seopseom 183 taxa and Wa-do 46 taxa. Floristic richness was in order analyzed to be Seogeon-do 28.62, Ran-do 19.24, Daryeo-do 18.53, Wa-do 16.42, Munseom 16.14, Hyeongje-do 14.76, Seopseom 13.32, Beomseom 13.24, Chagwi-do 6.15, Saeseom 5.86 and Jigwi-do 2.51. Life form spectrum was surveyed to H-$D_5$-$R_4$-e; hemieryptophyte(H) 26.8%, not production seeds($D_5$) 54.4%, clonal growth by stolons and struck roots($R_4$) 46.9% and erect form(e) 30.3%. According to the life form spectrum hemieryptophyte dominated high portions, which suggests islet in jeju-do has hemieryptophytic climate and similar to middle parts in Korean Peninsular. However, were noticed by that it has more therophytes. As a result, Partially shows the characteristics change from the temperate climate to the warm temperate climate or a semitropical climate by a climatic change.

• Proceedings of the Korean Society of Crop Science Conference
• /
• 2017.06a
• /
• pp.31-31
• /
• 2017

Understanding the mechanism(s) to overcome or prevent seed ageing deterioration during storage is of fundamental interest to seed physiologists. Vitamin E (tocols) is known as a key metabolite to efficiently scavenge lipid peroxy radicals which cause membrane breakdown resulting in seed ageing. However, in rice research this hypothesis has been tested for very few lines only without considering intraspecific variation in genomic structure. Here, we present a correlation study between tocols and seed longevity using a diverse rice panel. Seeds of 20 rice accessions held in the International Rice Genebank at the International Rice Research Institute, representing aus, indica, temperate japonica and tropical japonica subpopulations, were used for tocols analysis (quantification of ${\alpha}$-, ${\beta}$-, ${\gamma}$-, ${\delta}$-tocopherol/tocotrienol by ultra performance liquid chromatography) and storage experiments at $45^{\circ}C$ and 10.9% seed moisture content (sample taken for germination testing every 3 days up to 60 days). To examine interactions between DNA sequences and phenotype, the 700k high-density single-nucleotide polymorphism marker data-set was utilized. Both seed longevity (time for viability to fall to 50%; $p_{50}$) and tocols content varied across subpopulations due to heterogeneity in the genetic architecture. Among eight types of tocol homologues, ${\alpha}$-tocopherol and ${\gamma}$-tocotrienol were significantly correlated with $p_{50}$ (negatively and positively, respectively). While temperate japonica varieties were most abundant in ${\alpha}$-tocopherol, indica varieties recorded 1.3 to 1.7-fold higher ${\gamma}$-tocotrienol than those of other subpopulations. It was highlighted that specific ratio of tocol homologues rather than total tocols content plays an important role in the seed longevity mechanism.