• Applied Biological Chemistry
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• 제25권4호
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• pp.201-205
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• 1982

감 과실(果實)의 탈삽기구(脫澁機構) 및 탄닌생성에 관한 조사의 일환으로 감엽(葉)의 탄닌함량의 변화 및 현미경적 관찰의 결과 삽시(澁枾)에서는 엽(葉)과 과실(果實)의 탄닌함량의 변화 경향이 유사하였으나, 감시(甘枾)에서는 많은 차이가 있었다. 엽(葉)의 탄닌함량 변화는 품종간에 큰 차이가 없었다. 엽(葉)의 상피세포(上皮細胞)는 과실(果實)의 탄닌세포와 같이 다량의 탄닌물질을 함유하고 있었으며 유관속(維管束) 부근에도 탄닌세포가 존재하였다. 사관부내(篩管部內)에는 탄닌물질이 이동하고 있었다. 이상의 결과 감엽(葉)의 탄닌물질과 과실(果實)의 탄닌물질은 밀접한 관계가 있음을 알 수 있었다.

• 한국식품과학회지
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• 제5권4호
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• pp.258-267
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• 1973

국내(國內) 도토리의 이용(利用)을 위하여 그의 성분분석(成分分析)을 실시하였고, 가공(加工)에 장해(障害)가 되고 있는 tannin 성분(成分)의 효소적(酵素的) 가수분해(加水分解)를 시도하여 다음과 같은 결과(結果)를 얻었다. 1) 도토리와 상수리에 대한 주요성분(主要成分)을 분석(分析)하였다. 도토리중의 tannin 함량을 $6.5{\sim}7.5%$이었다. 2) 배양액 중에 tannin 가수분해(加水分解) 효소(酵素)를 다량(多量)으로 생산(生産)하는 우수균주(優秀菌株)의 분리(分離)를 시도하여 활성(活性)이 강한 Aspergillus flavus와 Aspergillus sp. AN-11을 부패도토리로부터 얻었다. 3) Aspergillus flavus 및 Aspergillus sp. AN-11의 효소생산(酵素生産)을 위한 최적(最適) 배양조건(培養條件)을 결정(決定)하였다.

• Asian-Australasian Journal of Animal Sciences
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• 제19권12호
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• pp.1776-1783
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• 2006

Despite being a rich source of protein (28-34%), karanj (Pongamia glabra) cake is found to be bitter in taste and toxic in nature owing to the presence of flavonoid (karanjin), tannin and trypsin inhibitor, thereby restricting its safe inclusion in poultry rations. Feeding of karanj cake at higher levels (>10%) adversely affected the growth performance of poultry due to the presence of these toxic factors. Therefore, efforts were made to detoxify karanj cake by various physico-chemical methods such as dry heat, water washing, pressure cooking, alkali and acid treatments and microbiological treatment with Sacchraromyces cerevisiae (strain S-49). The level of residual karanjin in raw and variously processed cake was quantified by high performance liquid chromatography and tannin and trypsin inhibitor was quantified by titrametric and colorimetric methods, respectively. The karanjin, tannin and trypsin inhibitor levels in such solvent and expeller pressed karanj cake were 0.132, 3.766 and 6.550 and 0.324, 3.172 and 8.513%, respectively. Pressure-cooking of solvent extracted karanj cake (SKC) substantially reduced the karanjin content at a cake:water ratio of 1:0.5 with 30-minute cooking. Among chemical methods, 1.5% (w/w) NaOH was very effective in reducing the karanjin content. $Ca(OH)_2$ treatment was also equally effective in karanjin reduction, but at a higher concentration of 3.0% (w/w). A similar trend was noticed with respect to treatment of expeller pressed karanj cake (EKC). Pressure cooking of EKC was effective in reducing the karanjin level of the cake. Among chemical methods alkali treatment [2% (w/w) NaOH] substantially reduced the karanjin levels of the cake. Other methods such as water washing, dry heat, HCl, glacial acetic acid, urea-ammoniation, combined acid and alkali, and microbiological treatments marginally reduced the karanjin concentration of SKC and EKC. Treatment of both SKC and EKC with 1.5% and 2.0% NaOH (w/w) was the most effective method in reducing the tannin content. Among the various methods of detoxification, dry heat, pressure cooking and microbiological treatment with Saccharomyces cerevisiae were substantially effective in reducing the trypsin inhibitor activity in both SKC and EKC. Based on reduction in karanjin, in addition to tannin and trypsin inhibitor activity, detoxification of SKC with either 1.5% NaOH or 3% $Ca(OH)_2$, w/w) and with 2% NaOH were more effective. Despite the effectiveness of pressure cooking in reducing the karanjin content, it could not be recommended for detoxification because of the practical difficulties in adopting the technology as well as for economic considerations.

• Asian-Australasian Journal of Animal Sciences
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• 제12권6호
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• pp.891-894
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• 1999

A field experiment was conducted with Calliandra calothyrsus of fifteen provenances to evaluate the variation in the condensed tannin content of fresh leaves. Plants were raised in a nursery for three months and planted in the field. Experimental design was line planting of double hedgerows (3 m long plot, 1/2 meter interval, 45 plants in each plot) with 5 replicates. Plants were lopped every 5 months and samples (10 different plants within a plot; 4 newly flushed leaves, 4 partially expanded leaves and 4 mature full expanded leaves from each of 10 trees in a plot) were collected and were put on ice before transfer to the laboratory. Proanthocyanidine was extracted in aqueous acetone, and total extractable proanthcyanidine (TEPA) and total proanthocyanidine (TOPA) were measured. In addition, proximate analyses were made of different provenances. Crude protein contents varied from 19.74% to 16.45%; in vitro organic matter digestibility ranged from 36.09% to 23.32%. TEPA content ranged from 11.22 to 16.01 (measured as absorbance at $550nm,\;g^{-1}$ DM) and TOPA from 24.57 to 31.72 (measured as absorbance at $550nm,\;g^{-1}$ DM) TEPA and TOPA had a positive correlation, whereas crude protein content of Calliandra was negatively correlated with TEPA and TOPA. Provenances, such as Georgesville (Belize), local, Coban (Guatemala), Patulul (Guatemala), Bombana (Mexico) can be selected on the basis of nutritive value of fodder.

• Asian-Australasian Journal of Animal Sciences
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• 제25권10호
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• pp.1389-1394
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• 2012

Sheanut cake (SNC), expeller (SNE) and solvent extractions (SNSE) samples were evaluated to determine their suitability in animal feeding. The CP content was highest in SNSE (16.2%) followed by SNE (14.7%) and SNC (11.6%). However, metabolizable energy (ME, MJ/kg) was maximum in SNC (8.2) followed by SNE (7.9) and SNSE (7.0). The tannin phenol content was about 7.0 per cent and mostly in the form of hydrolyzable tannin (HT), whereas condensed tannin (CT) was less than one per cent. The in vitro gas production profiles indicated similar y max (maximum potential of gas production) among the 3 by-products. However, the rate of degradation (k) was maximum in SNC followed by SNE and SNSE. The $t^{1/2}$ (time taken for reaching half asymptote) was lowest in SNC (14.4 h) followed by SNE (18.7 h) and SNSE (21.9 h). The increment in the in vitro gas volume (ml/200 mg DM) with PEG (polyethylene glycol)-6000 (as a tannin binder) addition was 12.0 in SNC, 9.6 in SNE and 11.0 in SNSE, respectively. The highest ratio of $CH_4$ (ml) reduction per ml of the total gas, an indicator of the potential of tannin, was recorded in SNE (0.482) followed by SNC (0.301) and SNSE (0.261). There was significant (p<0.05) reduction in entodinia population and total protozoa population. Differential protozoa counts revealed that Entodinia populations increased to a greater extent than Holotricha when PEG was added. This is the first report on the antimethanogenic property of sheanut byproducts. It could be concluded that all the three forms of SN byproducts are medium source of protein and energy for ruminants. There is a great potential for SN by-products to be incorporated in ruminant feeding not only as a source of energy and protein, but also to protect the protein from rumen degradation and suppress enteric methanogenesis.

• Journal of the Korean Wood Science and Technology
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• 제34권5호
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• pp.29-41
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• 2006

This study was to investigate the effect of adding additive as tannin, rice husk and charcoal, for reducing the formaldehyde emission level, on the adhesion properties of urea-formaldehyde (UF) resin for particleboard. We controlled the hot-pressing time, temperature and pressure to determine the bonding strength and formaldehyde emission. Blends of various UF resin/additives (tannin, rice husk and charcoal) compositions were prepared. To determine and compare the effect of additives (tannin, rice husk and charcoal) content, 0, 5, 10 and 15%, by weight of UF resin, were used. $NH_4Cl$ as hardener added. To determine the level of formaldehyde emission, we used the desiccator, perforator and 20 L-small chamber method. The formaldehyde emission level decreased with increased additions of additive (except rice husk). Also, increased hot-pressing time decreased formaldehyde emission level. At a charcoal replacement ratio of only 15%, the formaldehyde emission level is under F ✩ ✩ ✩ ✩ grade (emit < $0.3mg/{\ell}$). Curing of the high tannin additive content in this adhesive system indicated that the bonding strength increased. But, in the case of rice husk and charcoal, the bonding strength was much lower due to the inorganic substance. Furthermore, rice husk was poor in bonding strength as well as formaldehyde emission than tannin and charcoal.

• Journal of the Korean Wood Science and Technology
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• 제47권3호
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• pp.253-264
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• 2019

This study characterized the chemical compounds in tannin from mangium (Acacia mangium) bark extract and determined the physical-mechanical properties of glued laminated timber (glulam) made from sengon (Falcataria moluccana), jabon (Anthocephalus cadamba), and mangium wood. The adhesives used to prepare the glulam were based on mangium tannin and phenol resorcinol formaldehyde resin. Five-layer glulam beams measuring $5cm{\times}6cm{\times}120cm$ in thickness, width, and length, respectively, were made with a glue spread of $280g/m^2$ for each glue line, cold pressing at $10.5kgf/cm^2$ for 4 h and clamping for 20 h. Condensed mangium tannin consisted of 49.08% phenolic compounds with an average molecular weight of 4745. The degree of crystallinity was 14.8%. The Stiasny number was 47.22%. The density and the moisture content of the glulams differed from those of the corresponding solid woods with mangium having the lowest moisture content (9.58%) and the highest density ($0.66g/cm^3$). The modulus of rupture for all glulam beams met the JAS 234-2003 standard but the modulus of elasticity and the shear strength values did not. Glulam beams made with tannin had high delamination under dry and wet conditions, but glulam made from sengon and jabon wood met the standard's requirements. All glulam beams had low formaldehyde emissions and were classified as $F^{****}$ for formaldehyde emissions according to the JAS 234 (2003) standard.

• 한국식품조리과학회지
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• 제7권1호
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• pp.27-34
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• 1991

This study attempted to set up reasonable extraction time of Omija that was put in water for the various components to soak out. Changes of free sugars, organic acids, reducing sugar, total acid and tannin in Omija with various extraction times were investigated (together with the analysis of each components in Omija fruit). 1. High Performance Liquid Chromatography showed fructose, glucose, and sucrose to be the major free sugars of the Omija fruit. Free sugars and reducing sugar value in Omija beverage increased gradually in according with the extraction time, and marked 75.6% per total free sugars and 82.1% per total reducing sugar at 12 hours. 2. Gas Chromatography showed lactic acid, oxalic acid, fumaric acid, levulinic acid, succinic acid, malic acid, citric acid and pyroglutamic acid to be the major organic acids of the Omija fruit. Organic acids and total acids value in Omija beverage increased gradually on proportion to extraction time, and marked 97.0% per total organic acids at 9 hours and 79.0% per total acids at 12 hours. 3. Tannin content in Omija beverage was increased when extraction time was longer but it showed a low percentage as compared with the reducing sugar and total acid. Tannin content marked 48.8% per total tannin at 12 hours. 4. Sensory evaluation revealed that !1 hours of extraction produced the best quality products based in taste, flavor, color and over-all acceptability, considering the data, it seems possible to conclude that the optimum of time for extraction of Omija to water is 9 hours.

• 한국식품저장유통학회지
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• 제4권1호
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• pp.45-51
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• 1997

In our studies on the role of $\beta$-galactosidase in fruit softening, significant difficulty, was encountered in our attempts to extract RNA from persimmon(Diospyros kaki L. cv. Fuyu) fruit due to astringency and tannin content. Initial, unsuccessful RNA extractions involved methods using guanidinium isothiocyanate/CsCl with and without polyvinylpyrrolidone(PVP), phenol/sodium lauryl sulfate(SDS), guanidinium hydrochloride, as well as polysomal RNA purification method that used 0.2 M Tris-HCI (pH 9.0) containing KCI, Mg-acetate, EDTA, $\beta$-mercaptoethanol, and sucrose. A method was devised which employed treatment of fruit with CO2 gas to diminish astringency prior to RNA extraction, followed by extraction of tissue powders with Proteinase K extraction buffer containing PVP and ascorbate at an alkaline pH. This procedure resulted in the removal of tannins and other polyphenolics and extraction of relatively large amount of high-quality RNA suitable for cDNA library construction and polymerase chain reaction(PCR). Futhermore, the procedure does not use the toxic and corrosive chemical guanidinium isothiocyanate or require ultracentrifugation.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2022년도 추계학술대회
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• pp.305-305
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• 2022

In this study, 139 Korean landrace sorghum germplasms collected in 10 different Korean regions were analyzed for tannin, total phenolic contents(TPC), and antioxidant activities using three assays including ABTS, DPPH, and FRAP. The Korean landrace sorghums showed significant variations of metabolite contents and antioxidant activities by collection regions. Gyeonggi sorghum had the highest tannin and TPC contents with 273.73%, 7.395mgGAE/g, respectively. Therefore, three assays of antioxidant activities of Gyeonggi sorghum, which is highly related to tannin and TPC, were also significantly higher than others. However, Gyeongnam sorghums showed the lowest tannin and TPC with 148.34% and 3.482mgGAE/g, thus, three antioxidnat activities showed the lowest levels. Three accessions(IT322580, IT340261 and IT329053) had the highest tannin levels over 400%, but, two accessions (IT322613, IT270366) showed less than 1% of tannin content. Four accessions (IT270349, IT286448, IT331878, and IT251882) showed the highest level of TPC over 9mgGAE/g. In this study, antioxidant activities with three assays were strongly related to tannins and TPC, and TPC showed higher significance than tannin with all three antioxidant activities. The germplasms with the highest overall antioxidant activity were IT270349, IT340261, and IT286448, which had the highest levels of tannin and TPC. The principal component analysis presented that 2 sub-populations were divided. Gyeonggi and Gangwon Yeongseo sorghums, which had high metabolite contents and antioxidant activity levels, were mainly included in group 1. Gyeongnam and Jeonnam sorghums, which had low metabolite and antioxidant activity levels, were mainly included in group 2. This results could contribute to discover breeding metarials for the development of functional sorghum varieties.