• Korean Journal of Plant Tissue Culture
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• v.22 no.5
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• pp.241-260
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• 1995

Transposons, present in the genomes of all living organisms, are genetic element that can change positions, or transpose, within the genome. Most genomes contain several kinds of transposable elements and the molecular details of the mechanisms by which these transposons move have recently been uncovered in many families of transposable elements. Transposition is brought about by an enzyme known as transposaese encoded by the autonomous transposon itself, but, in the unautonomous transposon lacking the gene encoding the transposase, movement occurs only at the presence of the enzyme encoded by the autonomous one. There are two types of transposition events, conservative and replicative transposition. In the former the transposon moves without replication, both strands of the DNA moving together from one place to the other while in the latter the transposition frequently involves DNA replication, so one copy of transposon remains at its original site as another copy insole to a new site. The insertion of transposon into a gene can prevent it expression whereas excision from the gene may restore the ability of the gene to be expressed. There are marked similarities between transposons and certain viruses having single stranded Plus (+) RNA genomes. Retrotransposons, which differ from the ordinary transposons in that they transpose via an RNA-intermediate, behave much like retroviruses and have a structure of integrated retrovial DNA when they are inserted to a new target site. An insertional mutagenesis called transposon-tagging is now being used in a number of plant species to isolate genes involved in developmental and metabolic processes which have been proven difficult to approach by the traditional methods. Attempts to device a transposon-tagging system based on the maize Ac for use in heterologous species have been made by many research workers.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.21 no.4
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• pp.737-743
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• 2017

The intelligent life-log system proposed in this paper is intended to identify and record a myriad of everyday life information as to the occurrence of various events based on when, where, with whom, what and how, that is, a wide variety of contextual information involving person, scene, ages, emotion, relation, state, location, moving route, etc. with a unique tag on each piece of such information and to allow users to get a quick and easy access to such information. Context awareness generates and classifies information on a tag unit basis using the auto-tagging technology and biometrics recognition technology and builds a situation information database. In this paper, we developed an active modeling method and an application that recognizes expressionless and smile expressions using lip lines to automatically record emotion information.

• Korean Journal of Plant Tissue Culture
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• v.21 no.6
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• pp.319-326
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• 1994

For the gene tagging of Armoracia rusticana, maize controlling element Ac and Ds were introduced into A.rusticana via Agrobacterium-mediated transformation method. We established an efficient in via regeneration and transformation system for gene transfer in A. rusticana. The optimum in via regeneration condition has been obtained from leaf, petiole and root organs on modified MS medium supplemented with NAA 0.1 mg/L plus BA 1.0 mg/L for direct shooting and with free growth regulators for root induction for transformation, the leaf, petiole and root explants of A. rusticana were concultivated with Agrobacterium tumefaciens, LBA4404 which carries a binary vector pEND4K containing maize controlling element Ac or Ds, respectively: Selections were performed in the shoot induction medium supplemented with 100 mg/L kanamycin, and 500 mg/L carbenicillin transformation frequency showed about 8 to 10% in case of leaf disks. PCR md Southern blot analyses showed that the Ac and the Ds elements were integrated into the chromosome of donor plants.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.19 no.1
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• pp.289-294
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• 2019

It easily collects information on purchased goods using IoT(Internet of Things). The collected data is updated directly to the smartphone for verification. The payment information is generated by QR-Code. As a way to implement a system, System was configured with two assumptions: IoT technology using Raspberry-Pi and mobile QR technology. First, RFID tags are attached to the goods instead of barcodes. Second, it has an IoT computer(Raspberry-Pi) built into its shopping cart. This system keeps traditional shopping method of face-to-face payment, but replace time-consuming tarditional barcode tagging method to QR-tagging system for time-efficiency. By debeloping the system of this paper, we maintain pleasures in offline store shopping and it provide convenience due to reduced waiting time for customers and providing prior information about the products.

• International conference on construction engineering and project management
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• 2020.12a
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• pp.149-156
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• 2020

Blast furnace revamping in steel industry is one of the most important work to complete the complicated equipment within a short period of time based on the interfaces of various types of work. P company has planned to build a Smart Tracking System based on the wireless tag system with the aim of complying with the construction period and reducing costs, ahead of the revamping of blast furnace scheduled for construction in February next year. It combines the detailed design data with the wireless recognition technology to grasp the stage status of design, storage and installation. Then, it graphically displays the location information of each member in relation to the plan and the actual status in connection with Building Information Modeling (BIM) 4D Simulation. QR Code is used as a wireless tag in order to check the receiving status of core equipment considering the characteristics of each item. Then, DB in server system is built, status information is input. By implementing BIM 4D Simulation data using DELMIA, the information on location and status is provided. As a feature of the S/W function, a function for confirming the items will be added to the cellular phone screen in order to improve the accuracy of tagging of the items. Accuracy also increases by simultaneous processing of storage and location tagging. The most significant effect of building this system is to minimize errors in construction by preventing erroneous operation of members. This system will be very useful for overall project management because the information about the position and progress of each critical item can be visualized in real time. It could be eventually lead to cost reduction of project management.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.35 no.1B
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• pp.78-84
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• 2010

A fixed 900 MHz near field RFID system is developed to provide information; market efficiency, jewelry information and the circulation history information in real time, to the customers. The developed RFID system for the jewelry management consists of a reader, antenna and a CPU in a integrated type. The system size is $38\;{\times}\;25\;{\times}\;19\;cm^3$, the operated frequency band of the reader antenna is 905 ~ 926 MHz. The maximum gain of the embedded reader antenna is 5.1 dBii(@ 910 MHz). Honeycomb tag manufactured by RSI Co. is suitable for the jewelry management than another other commercial near field tags. The tagging method and the tagging location of Honeycomb tag are suggested. In the suggested system, the maximum reading range is about 16 cm, and the zone with 100 % recognition rate is 10 cm from the radom of the reader antenna.

• Journal of Life Science
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• v.24 no.9
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• pp.995-1000
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• 2014

The studies of marine viruses in terms of viral isolation and detection have been limited due to the high mutation rate and genetic diversity of marine viruses. Of the modern methods currently used to detect marine viruses, serological methods based on enzyme-linked immunosorbent assay (ELISA) are the most common. They depend largely on the quality of the antibodies and on highly purified suitable antigens. Recently, a new experimental system for using viral capsid protein as an antigen has been developed using the yeast surface display (YSD) technique. In the present study, the capsid protein gene of the red-spotted grouper nervous necrosis virus (RGNNV) was expressed and purified via YSD and HA-tagging systems, respectively. Two regions of the RGNNV capsid protein gene, RGNNV1 and RGNNV2, were individually synthesized and subcloned into a yeast expression vector, pCTCON. The expressions of each RGNNV capsid protein in the Saccharomyces cerevisiae strain EBY100 were indirectly detected by flow cytometry with fluorescently labeled antibodies, while recognizing the C-terminal c-myc tags encoded by the display vector. The expressed RGNNV capsid proteins were isolated from the yeast surface through the cleavage of the disulfide bond between the Aga1 and Aga2 proteins after ${\beta}$-mercaptoethanol treatment, and they were directly detected by Western blot using anti-HA antibody. These results indicated that YSD and HA-tagging systems could be applicable to the expressions and purification of recombinant RGNNV capsid proteins.

• Journal of Korea Multimedia Society
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• v.13 no.8
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• pp.1245-1255
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• 2010

In current Web 2.0 environment, one of the most core technology is social bookmarking which users put tags and bookmarks to their interesting Web pages. The main purpose of social bookmarking is an effective information service by use of retrieval, grouping and share based on user's bookmark information and tagging result of their interesting Web pages. But, current social bookmarking system uses the number of bookmarks and tag information separately in information retrieval, where the number of bookmarks stand for user's degree of interest on Web contents, information retrieval, and classification serve the purpose of tag information. Because of above reason, social bookmarking system does not utilize effectively the bookmark information and tagging result. This paper proposes a Web contents ranking algorithm combining bookmarks and tag information, based on preceding research on associative tag extraction by tag clustering. Moreover, we conduct a performance evaluation comparing with existing retrieval methodology for efficiency analysis of our proposed algorithm. As the result, social bookmarking system utilizing bookmark with tag, key point of our research, deduces a effective retrieval results compare with existing systems.

• Journal of KIISE:Computing Practices and Letters
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• v.15 no.6
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• pp.405-413
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• 2009

Tagging system is the system that allows internet users to assign new meta-data which is called tag to article, photo, video and etc. for facilitating searching and browsing of web contents. Tag cloud, a visual interface is widely used for browsing tag space. Tag cloud selects the tags with the highest frequency and presents them alphabetically with font size reflecting their popularity. However the conventional tag selection method includes known weaknesses. So, we propose a novel tag selection method Freshness, which helps to find fresh web contents. Freshness is the mean value of Kullback-Leibler divergences between each consecutive change of tag co-occurrence probability distribution. We collected tag data from three web sites, Allblog, Eolin and Technorati and constructed the system, 'Fresh Tag Cloud' which collects tag data and creates our tag cloud. Comparing the experimental results between Fresh Tag Cloud and the conventional one with data from Allblog, our one shows 87.5% less overlapping average, which means Fresh Tag Cloud outperforms the conventional tag cloud.

• Journal of Korea Multimedia Society
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• v.14 no.4
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• pp.567-576
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• 2011

In current Web 2.0 environment, one of the most core technology is social bookmarking which users put tags and bookmarks to their interesting Web pages. The main purpose of social bookmarking is an effective information service by use of retrieval, grouping and share based on user's bookmark information and tagging result of their interesting Web pages. But, current social bookmarking system uses the number of bookmarks and tag information separately in information retrieval, where the number of bookmarks stand for user's degree of interest on Web contents, information retrieval, and classification serve the purpose of tag information. Because of above reason, social bookmarking system does not utilize effectively the bookmark information and tagging result. This paper proposes a Web contents ranking algorithm combining bookmarks and tag information, based on preceding research on associative tag extraction by tag clustering. Moreover, we conduct a performance evaluation comparing with existing retrieval methodology for efficiency analysis of our proposed algorithm. As the result, social bookmarking system utilizing bookmark with tag, key point of our research, deduces a effective retrieval results compare with existing systems.