• Title/Summary/Keyword: tagging system

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Molecular Characterization of Adenylyl Cyclase Complex Proteins Using Versatile Protein-Tagging Plasmid Systems in Cryptococcus neoformans

  • So, Yee-Seul;Yang, Dong-Hoon;Jung, Kwang-Woo;Huh, Won-Ki;Bahn, Yong-Sun
    • Journal of Microbiology and Biotechnology
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    • v.27 no.2
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    • pp.357-364
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    • 2017
  • In this study, we aimed to generate a series of versatile tagging plasmids that can be used in diverse molecular biological studies of the fungal pathogen Cryptococcus neoformans. We constructed 12 plasmids that can be used to tag a protein of interest with a GFP, mCherry, $4{\times}FLAG$, or $6{\times}HA$, along with nourseothricin-, neomycin-, or hygromycin-resistant selection markers. Using this tagging plasmid set, we explored the adenylyl cyclase complex (ACC), consisting of adenylyl cyclase (Cac1) and its associated protein Aca1, in the cAMP-signaling pathway, which is critical for the pathogenicity of C. neoformans. We found that Cac1-mCherry and Aca1-GFP were mainly colocalized as punctate forms in the cell membrane and non-nuclear cellular organelles. We also demonstrated that Cac1 and Aca1 interacted in vivo by co-immunoprecipitation, using $Cac1-6{\times}HA$ and $Aca1-4{\times}FLAG$ tagging strains. Bimolecular fluorescence complementation further confirmed the in vivo interaction of Cac1 and Aca1 in live cells. Finally, protein pull-down experiments using $aca1{\Delta}$::ACA1-GFP and $aca1{\Delta}$::ACA1-GFP $cac1{\Delta}$ strains and comparative mass spectrometry analysis identified Cac1 and a number of other novel ACC-interacting proteins. Thus, this versatile tagging plasmid system will facilitate diverse mechanistic studies in C. neoformans and further our understanding of its biology.

Performance Comparison Analysis on Named Entity Recognition system with Bi-LSTM based Multi-task Learning (다중작업학습 기법을 적용한 Bi-LSTM 개체명 인식 시스템 성능 비교 분석)

  • Kim, GyeongMin;Han, Seunggnyu;Oh, Dongsuk;Lim, HeuiSeok
    • Journal of Digital Convergence
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    • v.17 no.12
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    • pp.243-248
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    • 2019
  • Multi-Task Learning(MTL) is a training method that trains a single neural network with multiple tasks influences each other. In this paper, we compare performance of MTL Named entity recognition(NER) model trained with Korean traditional culture corpus and other NER model. In training process, each Bi-LSTM layer of Part of speech tagging(POS-tagging) and NER are propagated from a Bi-LSTM layer to obtain the joint loss. As a result, the MTL based Bi-LSTM model shows 1.1%~4.6% performance improvement compared to single Bi-LSTM models.

Noun Sense Identification of Korean Nominal Compounds Based on Sentential Form Recovery

  • Yang, Seong-Il;Seo, Young-Ae;Kim, Young-Kil;Ra, Dong-Yul
    • ETRI Journal
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    • v.32 no.5
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    • pp.740-749
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    • 2010
  • In a machine translation system, word sense disambiguation has an essential role in the proper translation of words when the target word can be translated differently depending on the context. Previous research on sense identification has mostly focused on adjacent words as context information. Therefore, in the case of nominal compounds, sense tagging of unit nouns mainly depended on other nouns surrounding the target word. In this paper, we present a practical method for the sense tagging of Korean unit nouns in a nominal compound. To overcome the weakness of traditional methods regarding the data sparseness problem, the proposed method adopts complement-predicate relation knowledge that was constructed for machine translation systems. Our method is based on a sentential form recovery technique, which recognizes grammatical relationships between unit nouns. This technique makes use of the characteristics of Korean predicative nouns. To show that our method is effective on text in general domains, the experiments were performed on a test set randomly extracted from article titles in various newspaper sections.

A knowledge-based pronunciation generation system for French (지식 기반 프랑스어 발음열 생성 시스템)

  • Kim, Sunhee
    • Phonetics and Speech Sciences
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    • v.10 no.1
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    • pp.49-55
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    • 2018
  • This paper aims to describe a knowledge-based pronunciation generation system for French. It has been reported that a rule-based pronunciation generation system outperforms most of the data-driven ones for French; however, only a few related studies are available due to existing language barriers. We provide basic information about the French language from the point of view of the relationship between orthography and pronunciation, and then describe our knowledge-based pronunciation generation system, which consists of morphological analysis, Part-of-Speech (POS) tagging, grapheme-to-phoneme generation, and phone-to-phone generation. The evaluation results show that the word error rate of POS tagging, based on a sample of 1,000 sentences, is 10.70% and that of phoneme generation, using 130,883 entries, is 2.70%. This study is expected to contribute to the development and evaluation of speech synthesis or speech recognition systems for French.

Tag Recommendation Algorithms in Tagging System (태깅 시스템의 태그 추천 알고리즘)

  • Kim, Hyun-Woo;Lee, Kang-Pyo;Kim, Hyoung-Joo
    • Journal of KIISE:Computing Practices and Letters
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    • v.16 no.9
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    • pp.927-935
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    • 2010
  • In the era of Web 2.0, users create a number of their own Web contents. So, multimedia search becomes much more important than ever. A tag is a simple keyword which describes the Web contents including URL, pictures, and videos. Tags perform a role of descriptors of Web contents and Web metadata properly. If the number of tagged Web data increases, users are more likely to find the desired search result because the system includes the Web contents which have richer Web metadata. However, the number of users who use tags as Web metadata is relatively small. Because of the cumbersome process of adding tags, or users do not know what to add for the better accessibility from the public. Given situation, tag recommendation, which helps the process of adding tags, has been studied to solve these problems. When a user adds some Web contents, the tag recommendation system recommends relevant tags for the Web contents to the use, and the user selects recommended tags. We analyze and categorize various tag recommendation algorithms in tagging system.

협력적 태그를 이용한 추천 시스템

  • Yeon, Cheol;Kim, Heung-Nam;Ji, Ae-Tti;Jo, Geun-Sik
    • Proceedings of the Korea Inteligent Information System Society Conference
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    • 2007.05a
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    • pp.179-188
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    • 2007
  • 디지털 기기 가 보편 화 되 면서 많 은 디지털 컨텐츠가 생성되고 있다. 또한, 인터넷 서비스의 발전으로 이들 컨텐츠를 과거에 비해 손쉽게 웹 상에 개제할 수 있게 되 었다. 따라서, 많은 컨텐츠를 추 천해 주기 위해 추천 시스템에 관한 연구가 활발히 진행되고 있다. 이들 컨텐츠가 기존의 텍스트 기반에서 사진이나 동영상, 사운드 등 컴퓨터가 자동으로 내용을 파악하기 힘든 컨텐츠로 변화하면서, 내용의 파악이 필요 없 는 협력적 여 과(Collaborative Filtering)가 추천 시스템에서 유 용하게 이 용될 수 있다. 또한 web 2.0의 영향으로 컨텐츠를 분류하고 재검색을 용이하게 하기 위해 태깅(tagging)을 제공하는 서비스가 많아지고 있다. 본 논문에서는 내용 파 악이 힘든 컨텐츠의 효과적인 추천을 위해 협력적 여과(Collaborative Filtering)와 협력적 태깅(Collaborative Tagging)을 접목시킨 방법을 제안하고, 전통적인 협력적 여과 방법과 제안한 방법의 비교 실험을 통하여 협력적 여과 방법에서의 태 깅의 효과에 대 해 논한다.

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Color & Texture Attribute Classification System of Fashion Item Image for Standardizing Learning Data in Fashion AI (패션 AI의 학습 데이터 표준화를 위한 패션 아이템 이미지의 색채와 소재 속성 분류 체계)

  • Park, Nanghee;Choi, Yoonmi
    • Journal of the Korean Society of Clothing and Textiles
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    • v.44 no.2
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    • pp.354-368
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    • 2020
  • Accurate and versatile image data-sets are essential for fashion AI research and AI-based fashion businesses based on a systematic attribute classification system. This study constructs a color and texture attribute hierarchical classification system by collecting fashion item images and analyzing the metadata of fashion items described by consumers. Essential dimensions to explain color and texture attributes were extracted; in addition, attribute values for each dimension were constructed based on metadata and previous studies. This hierarchical classification system satisfies consistency, exclusiveness, inclusiveness, and flexibility. The image tagging to confirm the usefulness of the proposed classification system indicated that the contents of attributes of the same image differ depending on the annotator that require a clear standard for distinguishing differences between the properties. This classification system will improve the reliability of the training data for machine learning, by providing standardized criteria for tasks such as tagging and annotating of fashion items.

A Study of User Interests and Tag Classification related to resources in a Social Tagging System (소셜 태깅에서 관심사로 바라본 태그 특징 연구 - 소셜 북마킹 사이트 'del.icio.us'의 태그를 중심으로 -)

  • Bae, Joo-Hee;Lee, Kyung-Won
    • 한국HCI학회:학술대회논문집
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    • 2009.02a
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    • pp.826-833
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    • 2009
  • Currently, the rise of social tagging has changing taxonomy to folksonomy. Tag represents a new approach to organizing information. Nonhierarchical classification allows data to be freely gathered, allows easy access, and has the ability to move directly to other content topics. Tag is expected to play a key role in clustering various types of contents, it is expand to network in the common interests among users. First, this paper determine the relationships among user, tags and resources in social tagging system and examine the circumstances of what aspects to users when creating a tag related to features of websites. Therefore, this study uses tags from the social bookmarking service 'del.icio.us' to analyze the features of tag words when adding a new web page to a list. To do this, websites features classified into 7 items, it is known as tag classification related to resources. Experiments were conducted to test the proposed classify method in the area of music, photography and games. This paper attempts to investigate the perspective in which users apply a tag to a webpage and establish the capacity of expanding a social service that offers the opportunity to create a new business model.

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Interaction-based Collaborative Recommendation: A Personalized Learning Environment (PLE) Perspective

  • Ali, Syed Mubarak;Ghani, Imran;Latiff, Muhammad Shafie Abd
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • v.9 no.1
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    • pp.446-465
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    • 2015
  • In this modern era of technology and information, e-learning approach has become an integral part of teaching and learning using modern technologies. There are different variations or classification of e-learning approaches. One of notable approaches is Personal Learning Environment (PLE). In a PLE system, the contents are presented to the user in a personalized manner (according to the user's needs and wants). The problem arises when a new user enters the system, and due to the lack of information about the new user's needs and wants, the system fails to recommend him/her the personalized e-learning contents accurately. This phenomenon is known as cold-start problem. In order to address this issue, existing researches propose different approaches for recommendation such as preference profile, user ratings and tagging recommendations. In this research paper, the implementation of a novel interaction-based approach is presented. The interaction-based approach improves the recommendation accuracy for the new-user cold-start problem by integrating preferences profile and tagging recommendation and utilizing the interaction among users and system. This research work takes leverage of the interaction of a new user with the PLE system and generates recommendation for the new user, both implicitly and explicitly, thus solving new-user cold-start problem. The result shows the improvement of 31.57% in Precision, 18.29% in Recall and 8.8% in F1-measure.

Morpheme Recovery Based on Naïve Bayes Model (NB 모델을 이용한 형태소 복원)

  • Kim, Jae-Hoon;Jeon, Kil-Ho
    • The KIPS Transactions:PartB
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    • v.19B no.3
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    • pp.195-200
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    • 2012
  • In Korean, spelling change in various forms must be recovered into base forms in morphological analysis as well as part-of-speech (POS) tagging is difficult without morphological analysis because Korean is agglutinative. This is one of notorious problems in Korean morphological analysis and has been solved by morpheme recovery rules, which generate morphological ambiguity resolved by POS tagging. In this paper, we propose a morpheme recovery scheme based on machine learning methods like Na$\ddot{i}$ve Bayes models. Input features of the models are the surrounding context of the syllable which the spelling change is occurred and categories of the models are the recovered syllables. The POS tagging system with the proposed model has demonstrated the $F_1$-score of 97.5% for the ETRI tree-tagged corpus. Thus it can be decided that the proposed model is very useful to handle morpheme recovery in Korean.