• Journal of Nutrition and Health
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• v.50 no.3
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• pp.246-256
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• 2017

Purpose: This study aimed to investigate the association of total dietary antioxidant capacity (TAC) with oxidative stress and metabolic markers among patients with metabolic syndrome according to gender. Methods: A total of 346 subjects aged 30~59 years with two or more risk factors of metabolic syndrome were recruited from a general hospital near Seoul in South Korea between 2010 and 2012 based on data from the medical checkup. Biochemical indices for oxidative stress and metabolic markers were measured. Food consumption data from 3-day food records were linked with the antioxidant capacity database for commonly consumed Korean foods to estimate individual's TAC. Results: Average dietary TAC of the study subjects was 132.0 mg VCE/d/1,000 kcal in men and 196.4 mg VCE/d/1,000 kcal in women. Levels of ${\gamma}$-glutamyltransferase (GGT), systolic blood pressure, diastolic blood pressure, and blood triglycerides were reduced significantly according to increasing TAC in men, but there was no significant trend in women. Intakes of total flavonoids and carotenoids were significantly negatively correlated with GGT (p < 0.05) and d-ROMs (p < 0.01) in men, whereas those of ${\alpha}$-tocopherol (p < 0.05) and ${\gamma}$-tocopherol (p < 0.05) were positively correlated with biological antioxidant potential (BAP) in women. The odds ratio of high oxidative stress indices and abnormal metabolic markers according to TAC level were not significant in either men or women. Conclusion: The results show that dietary TAC was partially associated with oxidative stress and metabolic markers among patients with metabolic syndrome. Further research is required for elucidating the association between dietary TAC and incidence of metabolic syndrome and chronic diseases within a large population in prospective studies.

• The Korean Nurse
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• v.14 no.1 s.75
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• pp.19-21
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• 1975

• Biotechnology and Bioprocess Engineering:BBE
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• v.1 no.1
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• pp.9-12
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• 1996

In order to produce the single chain precursor of a novel human insulin analogue, (B30-Homoserine) insulin, the fermentative behaviors of Escherichia coli JM103 were studied, which harbors pKBA plasmid carrying a hybrid gene in which the gene for a single chain precursor was fused with lacZ gene under tac promoter. The maximal induction of gene expression was achieved when more than 0.05 mM of isopropyl-$\beta$-D-thiogalactopyranoside(IPTG) was supplemented to fermentation medium after 4 h cultivation of E. coli, and followed by longer than 2-h fermentation. The hybrid protein of the single chain insulin precursor was isolated from cytoplasmic inclusion bodies by dissolving in 8M urea solution, and purified through DEAE-Sephacel and Sephadex G-200 column chromatographies with a recovery of 35%. The finally purified hybrid protein showed a single band on sodium dodecyl sulfate-polyacrylamide gel.

• 한국연소학회:학술대회논문집
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• 2014.11a
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• pp.281-282
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• 2014

Recently, studies on Mild combustion have grown in many combustion application fields in the international combustion society. Compared with international activities in this field, domestic study in Korea has not been activated yet. This brief review aims to explain some essences of fundamental physics of Mild combustion and to introduce some recent application techniques of them. Fundamental physics of Mild combustion has been usually broken down into three aspects [1]; physical, thermodynamic, and chemical aspects. A major portion of Mild combustion physics is related to HiTAC (High Temperature Air Combustion) or HiCOT (High Temperature Combustion Technology). Although definition of Mild combustion is easily accepted among combustion engineers, combustion control in Mild combustion may be difficult without understanding essential physics of it. To encourage the research in this field, some representative cases will be introduced, and related essential techniques will be explained.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
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• v.21 no.12
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• pp.1101-1104
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• 2008

We have studied electro-optic characteristics to improve viewing angle of the in-plain switching (IPS) liquid crystal display using an compensation film. The IPS mode shows relatively large light leakage and color shift in diagonal directions in a dark state. To solve this problem, we have compensated the low contrast ratio in diagonal directions using one optimized discotic film and adjusting TAC films of polarizers. The compensated IPS mode shows wide viewing angle characteristics that region of CR 50:1 is over $60^{\circ}$ of polar angle in all directions. The optimized IPS cell exhibits much better performances than other methods do in terms of CR and color uniformity.

• Journal of Microbiology and Biotechnology
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• v.18 no.2
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• pp.334-337
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• 2008

A tightly regulated gene expression system composed of a single-copy target gene under the control of a lac promoter derivative and lacI gene in a multicopy plasmid is proposed, and its ability to control the flux of a metabolic pathway is demonstrated. A model system to control the flux of acetyl-CoA to acetyl phosphate was constructed by integrating pta, a gene encoding phosphotransacetylase, under a tac promoter into the chromosome of E. coli with a pta-negative background and transforming a multicopy plasmid containing the $lacI^Q$ gene into the strain. The production rate of acetate was shown to be tightly controlled when varying the concentration of the inducer (IPTG) in he model system.

• Ocean and Polar Research
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• v.25 no.4
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• pp.483-491
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• 2003

Since the red grouper was declared overfished, the Gulf of Mexico Fishery Management Council must prepare a rebuilding plan considering the following alternative management policies: a Total Allowable Catch (TAC), 5-month season closure, 1800-pound trip limit, and a 50-fathom longline boundary. This study was aimed at evaluating the effects of proposed policies for rebuilding the red grouper stock in a 10-year period by developing a bioeconomic model. Under the assumption that the recreation sector was held to its share of TAC (24% of the total quota), the target stock biomass goal was attained in all policies. The NPV was the largest in the 5-month season closure policy if the output price did not fall. There were distributional effects on the different components of the fleets in the 1800-pound trip limit and the 50-fathom longline boundary policy.

• Korean Journal of Microbiology
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• v.29 no.1
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• pp.1-7
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• 1991

Human immunodeficiency virus type 1 (HIV-1) causes a deadly infectious disease, Acquired Immunodeficiency Syndrome (ADIS). As a first step to develop a reliable and fast diagnostic procedure for HIV-1 infection, we cloned various immunodominant epitopes of HIV-1 in bacterial expression vectors containing tac or trp promoter. While the protein level of direct expression of gp160 was low, trp E fused gp120, gp41 and p17-p24 were produced at high levels (15-30% of total bacterial proteins) in E. coli. Since gp120 and gp41 contain relatively conserved regions which can react with antibodies in the plasma from most of HIV-1 infected individuals, these expression clones were used for large preparations of HIV-1 antigens.

• Proceedings of the Korean Fiber Society Conference
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• 1993.06b
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• pp.617-621
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• 1993

• Development and Reproduction
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• v.20 no.1
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• pp.51-61
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• 2016

Neurokinin B (NKB) and neurokinin B related peptide (NKBRP) belong to tachykinin peptide family. They act as a neurotransmitter and/or neuromodulator. Mutation of NKB and/or its cognate receptor, NK3R resulted in hypogonadotropic hypogonadism in mammals, implying a strong involvement of NKB/NK3R system in controlling mammalian reproduction. Teleosts possess NKBRP as well as NKB, but their roles in fish reproduction need to be clarified. In this study, NKB and NKBRP coding gene (tac3) was cloned from Nile tilapia and sequenced. Based on the sequence, Nile tilapia NKB and NKBRP peptide were synthesized and their biological potencies were tested in vitro pituitary culture. The synthetic NKBRP showed direct inhibitory effect on the expression of GTH subunits at the pituitary level. This inhibitory effect was confirmed in vivo by means of intraperitoneal (ip) injection of synthetic NKB and NKBRP to mature female tilapia (20 pmol/g body weight [BW]). Both NKB and NKBRP had no effect on the plasma level of sex steroids, E2 and 11-KT. However, NKBRP caused declines of expression level of GnRH I, Kiss2 and tac3 mRNAs in the brain while NKB seemed to have no distinct effect. These results indicate some inhibitory roles of NKBRP in reproduction of mature female Nile tilapia, although their exact functions are not clear at the moment.