• The Journal of Fisheries Business Administration
• /
• v.26 no.1
• /
• pp.41-54
• /
• 1995

Recently our government intended to change the present fisheries management system into the allocated catch quota system, which is to increase the income of fishermen and to recover the depleted resources up to a desirable level. This paper is to find out the difficulties of introducing the allocated catch quota system into our fisheries mangement system and to reduce the possible errors for the enforcement of this system. The allocated catch quota system can be devided by two kinds, the one is to allocate among industries or fisheries with total allowable catch(TAC) and the other is to allocate among fishermen or individual vessels with individual fish quota(ITQ). The latter is a much advanced control system compared with the former and is what this study treats. This paper reviewed the case of Newzealand and Canada where the ITQ system is introduced earlier and classified the problems by two parts for successful introduction into Korea as follows : (1) allocation method problems, (2) enforcement problems. For the first part the problems to be considered are 1) a scientific oath qouta calculation system is necessary, 2) the quota must be opened, 3) by-catch problems. 4) interactions with adjoining countries. For the second part the problems to be considered are 1) monitoring system, 2) quota transferability, 3) quota flexibility, 4) the enforcement of the system must be connected with the reduction of fishing power, 5) a mass communication and decision making system between government and fishermen is essential.

• The Journal of Information Systems
• /
• v.19 no.1
• /
• pp.79-96
• /
• 2010

The organizations in the competitive business environment are under pressure of handling internal management processes as well as external supply chain issues. In addition, the paradigm of supply chain management (SCM) has been shifted from static to dynamic problems. For the efficient SCM, organizations should be a RTE (Real Time Enterprises) that views entire supply chains as a single entity. In this paper, the agent system that makes RTE and dynamic supply chains possible is developed. Then, the developed agent system is verified in the TAC SCM(Trading Agent Competition Supply Chain Management) experimental environment and a dashboard is also developed for monitoring management activities of supply chains. The contributions of this paper are as followings. First, unlike the previous agent systems, the suggested agent system in this paper provides autonomy and scalability using SOA. Second, this suggested model for dynamic environment can be applied for the development of software supporting a SCM.

• Journal of Environmental Science International
• /
• v.18 no.11
• /
• pp.1207-1214
• /
• 2009

Functional properties of Solanium nigrum-ethanol extract were investigated, nitrite scavenging ability(NSA) and antioxidant activities based on thiobarbituric acid(TBA) value were measured. The contents of potassium in fruit and root, calcium in leaf were the highest mineral components in each parts of Solanium nigrum. Total aromatics contents(TAC) was order of fruit>root>leaf, total flavonoid contents(TFC) in leaf was 6 and 10 times, total phenolic compounds contents(TPC) in leaf was 15 and 30 times of those in fruit and root, respectively, and TFC was high with TPC increase. NSA of leaf-ethanol extract was 61.9% on pH 1.2, 53.5% on pH 3.0, 54.8% on pH 4.0 and 14.3% on pH 6.0, where as NSA of root and fruit-ethanol were considerably low to 3.5~7.9% over pH 1.2~6.0. TBA value of soybean oil with the dosage of leaf-extract showed few differences from control within 3 days, but it fairly decreased with considerable antioxidant effect to 47.6% of the control in 8 days.

• Proceedings of the IEEK Conference
• /
• 2002.06b
• /
• pp.129-132
• /
• 2002

AE64000 is the 64-bit high-performance microprocessor that ADC Co. Ltd. is developing for an embedded environment. It has a 5-stage pipeline and uses Havard architecture with a separated instruction and data caches. It also provides SIMD-like DSP and FP operation by enabling the 8/16/32/64-bit MAC operation on 64-bit registers. AE64000 processor implements the EISC ISA and uses the instruction folding mechanism (Instruction Folding Unit) that effectively deals with LERI instruction in EISC ISA. But this unit makes branch prediction behavior difficult. In this paper, we designs a branch predictor optimized for AE64000 Pipeline and develops a AES4000 simulator that has cycle-level precision to validate the performance of the designed branch predictor. We makes TAC(Target address cache) and BPT(branch prediction table) seperated for effective branch prediction and uses the BPT(removed indexed) that has no address tags.

• Archives of Pharmacal Research
• /
• v.27 no.3
• /
• pp.319-323
• /
• 2004

Cytochrome P450 (CYP) 3A4 is of great interest because of its important roles in the oxidation of numerous drugs and xenobiotics. HDJ-1, a molecular chaperone in human, is known to assist the correct folding of unfolded proteins. To achieve a high yield of recombinant human CYP3A4 in Escherichia coli, the CYP3A4 encoding gene was co-expressed with the chaperone HDJ-1, under the control of an inducible tac promoter in a bicistronic format. The levels of expression of the CYP3A4 in the bicistronic construct reached up to 715 nmol $(liter culture)^{-1}$ within 16 h at $37^{\circ}C$, which was about a 3.3-fold increase compared to that of the CYP3A4 alone without the HDJ-1. By co-expression with HDJ-1, the catalytic activity of CYP3A4 was also increased by -15-fold. The amount of activity increase was similar to that of the CYP production at the whole cell level. The present over-expression system may be useful for the rapid production of large amounts of active CYP3A4 in E. coli.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.49 no.5
• /
• pp.648-656
• /
• 2016

The age and growth of jack mackerel Trachurus japonicus caught by large purse seine fishery were studied during August 2004 and May 2005. Otoliths of 472 fish were observed under reflected light, and translucent zones made on the post-rostrum axis were regarded as annual marks. Monthly changes in the marginal index indicated that the translucent zone was formed once a year, mainly in March-April, which was coincident with the peak spawning time of jack mackerel. The estimated ages were 0-6 years, corresponding to a fork length (FL) range of 13 to 38 cm. Most obtained individuals were 2-year-old fish, followed by 1-year-old fish. The mean fork length at age from otolith reading (sex combined) was applied to the von Bertalanffy equation and growth parameters were estimated: L_∞ = 38.29 cm FL, k = 0.31 years⁻¹, t₀ = −0.81 years.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
• /
• 2008.10a
• /
• pp.479-482
• /
• 2008

사고력 신장 교육은 정보영재를 위한 교육과정 및 내용 중에서 매우 중요한 목표이다. 이 논문에서는 정보영재 교육에서 중요성이 증대되는 사고력 신장 교육에 적합한 교수-학습 프로그램을 제시하여 적용하고 분석하는 방안을 마련한다. 사고력 신장 교육을 구성하는 영역별 교육 내용에 포함되는 여러 가지의 문제들에 공통적으로 적용할 수 있는 모형으로서 AND/OR 트리에 기반을 둔 교수-학습 모형을 제시한다. 이 논문에서 제시된 AND/OR 트리형 교수-학습 모형을 정보영재의 현장 교육에 도입하기 위하여 tic-tac-toe 게임 문제를 예로 들어 적용 방안을 제시하며, 그 적용 결과를 분석하여 교육 프로그램 개발의 타당성을 검증한다. AND/OR 트리형 교수-학습 모형에서는 AND 트리와 OR 트리가 결합된 형태의 트리 구성과 그에 따른 트리 탐색을 주요 학습 내용으로 하는 고도의 사고력을 필요로 하는 학습 단계를 필요로 한다.

• The Journal of the Korean Society for Microbiology
• /
• v.35 no.2
• /
• pp.109-115
• /
• 2000

FtsH is a membrane-bound, ATP-dependent metalloprotease that is involved in a variety of cellular functions including the regulation of responses to heat and stress shock. Previously, we had cloned and sequenced pneumococcal ftsH gene whose deduced amino acid sequence was very similar to those of several gram-positive bacteria and Escherichia coli, except for the N-terminal domain that was responsible for membrane anchoring. In order to better understand the role of Streptococcus pneumoniae FtsH, we expressed pneumococcal ftsH gene in Escherichia coli. When it was expressed from a strong promoter, $P_{tac}$, a considerable amount of the recombinant FtsH was produced, although the prolonged induction resulted in not only accumulation of breakdown products but also ceasing of the further growth of E. coli host. This indicated that the expression of the exogenous ftsH gene was tightly regulated since the excessive FtsH appeared detrimental to bacterial cells. In Western blotting, the pneumococcal FtsH protein, whether native or recombinant, was reactive to anti-E. coli FtsH serum. The observation that FtsH proteins were well conserved throughout the bacterial kingdom and its expression level was fine-tuned suggests an important role for this protein in the stress adaptation which may be related to infecting process by pneumococci.

• Molecules and Cells
• /
• v.25 no.3
• /
• pp.446-451
• /
• 2008

We assessed heterologous protein expression in 64 strains obtained from the Escherichia coli Reference (ECOR) collection, a collection representing diverse natural E. coli populations. A plasmid generating a glutathione S-transferase and plant carbonic anhydrase fusion protein (GST-CA) under the control of the tac promoter was introduced into the ECOR strains, and the quantity of the fusion protein was determined by SDS-PAGE. The foreign protein was generated at various levels, from very high (40 strains, high producers) to very low (six strains, low producers). Immunoblotting showed that the high producers expressed approximately 250-500 times more GST-CA protein than the low producers. The results of semi-quantitative RT-PCR showed that the low producers generated mRNA levels comparable to those of the high producers, thereby suggesting that, at least in this case, inefficient translation is a major cause of the low production. We introduced a different plasmid, which expressed a maltose binding protein and plant guanylate kinase fusion protein (MBP-GK) into the six low producers. Interestingly, five of these expressed MBP-GK at very high levels. Thus, we conclude that the production of a particular protein from an expression vector can vary considerably, depending on the host strain. Strains in the ECOR collection could function as useful alternative hosts when a desired level of protein expression is not obtained from commonly used strains, such as E. coli K12 or B derivatives.

• Korean Journal of Microbiology
• /
• v.31 no.1
• /
• pp.27-36
• /
• 1993

Rhizobium fredii USDA191 은 대기 중의 질소를 환원하여 식물체의 생육에 필요한 질소원을 공급해주는 세균으로 다량의 체외 다당류를 합성한다. 전위요소 Tn5의 삽입에 의한 돌연변이 유도로 다당류결핍 변이주 R. fredii YKL293 가 분리되었으며 이 변이주로부터 Tn5 에 인접한 DNA 단편이 pUC19 에 클로닝되었고(plyk5293),이 DNA 단편을 탐침으로 하여 .lambda.NM1149 에 구성되 USDA191 genomic library 로부터 야생형체외다당류 합성관련 유전자(exo) 를 함유한 클론 .lambda. NM1149 22E 를 plaque 혼성화에 의하여 분리하였다. 클론 NM1149.22E 에 들어있는 exo 유전자를 pBR322 에 옮겨서 pJW33을 만들고, 재조합체 pJW33 을 Escherichia coli POII734 에 도입시켜 lacZ 구조유전자를 함유한 MudI 1734 가 exo 유전자의 프러모토와 융합되어 lacZ 구조유전자의 전사가 이루어지도록 하였다. 위와 같이 만들어진 재조합체 플라스미드 pUM21을 함유한 E. coli JM83 은 .betha.-galactosidase 를 합성하였으며, 야생형 tacZ 유전자를 갖고 있는 E. coli LE392 에 비해서 14-25배 정도 낮은 역가를 보였다.