• Journal of the Korean Society of Industry Convergence
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• v.19 no.3
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• pp.133-143
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• 2016

The objective in here is to find the density, stiffness, and strength of truss-wall rectangular (TWR) model which is combined with lattice truss (MLT) inside space. The TWR unit-cell model is defined as a unit cell originated from a solid-wall rectangular (SWR) model and it has an empty space inside. Thus, the empty space inside of the TWR is filled with lattice truss model defined as TWR-MLT. The ideal solutions derived of TWR-MLT are based on TWR with MLT model and it has developed by Gibson-Ashby's theory. To validate the ideal solutions of the TWR-MLT, ABAQUS software is applied to predict the density, strength, and stiffness, and then each of them are compared with the Gibson-Ashby's ideal solution as a log-log scale. Applied material property is stainless steel 304 because of cost effectiveness and easy to get around. For the analysis, SWR and TWR-MLT models are 1mm, 2mm, and 3mm truss diameter separately within a fixed 20mm opening width. In conclusion, the relative Young's modulus and relative yield strength of the TWR-MLT unit model is reasonably matched to the ideal expectations of the Gibson-Ashby's theory. In nearby future, TWR-MLT model can be verified by advanced technologies such as 3D printing skills.t.

• Proceedings of the KIEE Conference
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• 2006.07b
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• pp.1004-1005
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• 2006

This paper investigates a speed sensorless control of induction motor. The control strategy is based on MRAS (Model Reference Adaptive System) using load-torque observer as a reference model for flux estimation. The speed response of conventional MRAS controller characteristics is affected by variations of load torque disturbance. In the proposed system, the speed control characteristics using a load-torque observer control isn't affected by a load torque disturbance. Simulation results are presented to prove the effectiveness of the adaptive sliding mode controller for the drive variable load of induction motor.

• Proceedings of the KIEE Conference
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• 2005.10a
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• pp.136-138
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• 2005

The conventional self-tuning methods have the speed control problem of nonlinear BLDC motor which can't adapt against any kinds of noise or operation circumstances. In this paper, supposed to solve these problem to PI parameters controller algorithm using ANN. In the proposed algorithm, the parameters of the controller were adjusted to reduce by on-line system the error of the speed of BLDC motor. In this process, EBPA NN was constituted to an output error value of a BLDC motor and conspired an input and output. The performance of the self-tuning controller is compared with that of the PI controller tuned by conventional method(Z&N). The effectiveness of the proposed control method IS verified thought the Matlab Simulink.

• Advances in concrete construction
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• v.4 no.1
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• pp.49-69
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• 2016

This work investigates the mechanical properties of conventional concrete (CC) and self compacting concrete (SCC) using fine rubber and silpozz were accompanied by a comparative study between conventional rubberized concrete (CRC) and self compacting rubberized concrete (SCRC). Fine rubber (FR) from scrap tires has replaced the fine aggregate (FA) and Silpozz has been used as a replacement of cement at the proportions of 5, 10 and 15%. Silpozz as a partial replacement of cement in addition of superplasticiser (SP) increases the strength of concrete. Fresh concrete properties such as slump test, compaction factor test for CRC, whereas for SCRC slump flow, $T_{500}$, V-funnel, L-box, U-box, J-ring tests were conducted along with the hardened properties tests like compressive, split tensile and flexural strength test at 7, 28 and 90 days of curing. The durability and microstructural behavior for both CRC and SCRC were investigated. FR used in the present study is 4.75 mm passing with fineness modulus 4.74.M30 grade concrete is used with a mix proportion of 1:1.44:2.91 and w/c ratio as 0.43. The results indicate that as FR quantity increases, workability of both CRC and SCRC decreases. The results also show that the replacement of natural fine aggregate (NFA) with FR particles decreases the compressive strength with the increase of flexural strength observed upto 5% replacement of FR. Also replacement of cement with silpozz resulted enhancement of strength in SCRC.

• Journal of the Korean Society of Food Science and Nutrition
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• v.43 no.9
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• pp.1342-1348
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• 2014

Seeds of Korean pine cone (Pinus koraiensis) have long been consumed as an edible food in countries located in North-East Asia, On the other hand, Korean pine cone, containing various polyphenols, is discarded as a useless garbage after removing seeds. This study investigated the lipolytic effects of pine cone extract in differentiated 3T3-L1 adipocytes. Intracellular lipid accumulation was measured by Oil red O staining, free glycerol release by colorimetric reaction, and expression of genes related to lipid metabolism by real-time PCR. Compared to control, pine cone extract reduced intracellular lipid accumulation by 8.8% and increased free glycerol release by 8.2% a concentration of $5{\mu}g/mL$ in differentiated 3T3-L1 adipocytes. mRNA levels of fatty acid synthesis were not significantly different between control and pine cone extract, but mRNA levels of lipoprotein lipase (LPL) and hormone-sensitive lipase (HSL) significantly increased by 38.7% and 94.1% at a concentration of $5{\mu}g/mL$, respectively. Thus, pine cone extract is suggested to have lipolytic effects through induction of LPL and HSL gene expression.

• Journal of Life Science
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• v.30 no.6
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• pp.532-541
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• 2020

In this study, we describe the inhibition of adipocyte differentiation by the lactic acid bacteria (LAB) fermentation product of Chrysanthemum indicum L. (CI) extract to control obesity. Preparation of LAB-fermented products was performed to overcome the cytotoxicity of CI extract. During fermentation and 3T3-L1 cell line experiment, cytotoxicity was not induced in the CI fermentation products over 1 day in culture. Fermented materials from highly proliferative cultures were selected for treatment of 3T3-L1 cells and for comparison with unfermented control groups. Cell survival and undifferentiated cell populations were decreased differentiation population in all experimental groups compared with controls, as measured using fluorescence-activated cell sorting analysis. Akt pathway activity increased upon treatment with these fermented extracts in 3T3-L1 cells. Gli2 depleted at the protein level in association with adipocyte differentiation. LAB KCTC 3115- and 3109-fermented extract treatment caused controlled Gli2 protein accumulation. Moreover, KCTC 3115 and 3109 were found to reduce C/EBPα and FAS was depleted, whereas pACC was increased at the protein level upon treatment with the fermentation products of each of the four LAB used in this study. With Lactococcus lactis subsp. lactis KCTC 3115 fermentation, the regulation of adipose differentiation and hedgehog signaling were also suppressed, thereby inhibiting the differentiation of progenitor cells. The basis for the activation of hedgehog signaling may provide insights into the treatment of obesity and the inhibition of adipocyte differentiation.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
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• v.27 no.11
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• pp.696-701
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• 2014

In this study, in order to develop coupled vibration mode piezoelectric devices for Acoustic Emission (abbreviated as AE) sensor application with outstanding displacement and piezoelectric properties have been simulated by ATILA FEM program. And, From the results of ATILA simulation, the AE sensor specimen, obtained superior electromechanical coupling factor and displacement, when the size of specimen is $3.45mm{\Phi}{\times}3.45mm$ with ratio of diameter/thickness(${\Phi}/T$)= 1.0. Therefore, AE sensor was fabricated by (Na,K,Li)(Nb,Ta) $O_3$(abbreviated as NKL-NT) system piezoelectric ceramics using coupled vibration mode. The piezoelectric properties of NKL-NT ceramics was exhibited that piezoelectric constant($d_{33}$), piezoelectric voltage constant($g_{33}$) and electro mechanical coupling factor($k_p$) have the excellent values of 261[pC/N], 40.10[$10^{-3}Vm/N$], and 0.44, respectively. The manufactured piezoelectric device with ratio of ${\Phi}/T$= 1.0 indicated the optimum values of resonant frequency(fr)= 556.5[kHz], antiresonant frequency(fa)=631.1[kHz], and effective electromechanical coupling factor(keff)= 0.473. The maximum sensitivity of the coupled vibration mode AE sensor was 55[dB] at the resonant frequency of 75[kHz]. The results show that the coupled vibration mode piezoelectric device is a promising candidate for the application AE sensor piezoelectric device.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.6
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• pp.2447-2451
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• 2014

Human papillomavirus (HPV) is the primary etiologic agent of cervical cancer. Consideration of safety and non human leukocyte antigen restriction, protein vaccine has become the most likely form of HPV therapeutic vaccine, although none have so far been reported as effective. Since tumor cells consistently express the two proteins E6 and E7, most therapeutic vaccines target one or both of them. In this study, we fabricated DC vaccines by transducing replication-defective recombinant adenoviruses expressing E6/E7 fusion gene of HPV-16, to investigate the lethal effects of specific cytotoxic T lymphocytes (CTL) against CaSki cells in vitro. Mouse immature dendritic cells (DC) were generated from bone marrow, and transfected with pAd-E6/E7 to prepare a DC vaccine and to induce specific CTL. The surface expression of CD40, CD68, MHC II and CD11c was assessed by flow cytometry (FCM), and the lethal effects of CTL against CaSki cells were determined by DAPI, FCM and CCK-8 methods. Immature mouse DC was successfully transfected by pAd-E6/E7 in vitro, and the transfecting efficiency was 40%-50%. A DC vaccine was successfully prepared and was used to induce specific CTL. Experimental results showed that the percentage of apoptosis and killing rate of CaSki cells were significantly increased by coculturing with the specific CTL (p <0.05). These results illustrated that a DC vaccine modified by HPV-16 E6/E7 gene can induce apoptosis of CaSki cells by inducing CTL, which may be used as a new strategy for biological treatment of cervical cancer.

• Journal of Life Science
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• v.22 no.5
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• pp.697-701
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• 2012

Activated protein C (APC) has an anticoagulant effect and a non-hemostatic effect such as regulation of cell metastasis and modulation of inflammation. In this study, we investigated whether APC could modulate apoptosis in cancer cells. Tumor necrosis factor (TNF)-${\alpha}$, cyclohexamide, and FAS markedly induced apoptosis in human renal carcinoma Caki cells. When Caki cells were pretreated with APC, the percentage of death receptor-induced apoptosis did not change. Furthermore, we checked the effect of APC on tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-induced apoptosis in human glioma T98G and human breast carcinoma MDA231 cells. APC also had no effect on TRAIL-induced apoptosis in both cell lines. However, pretreatment with APC inhibited combination treatment (kahweol plus TRAIL and kahweol plus melatonin)-induced apoptosis and PARP cleavage in Caki cells. Taken together, our results suggest that APC can modulate anti-cancer therapeutic efficiency.

• Asian Pacific Journal of Cancer Prevention
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• v.16 no.9
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• pp.4103-4107
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• 2015

Background: Hepatocellular carcinoma (HCC) is one of the most prevalent malignancies worldwide. Transarterial chemoembolisation (TACE) is the standardized therapy for intermediate stage HCC. However, the prognosis for HCC patients treated by TACE greatly varies. Thus, there is a critical need for finding biomarkers to predict the prognosis of HCC patients. The amino acid transporter-2 (ASCT2) is involved in tumorigenesis and progression of many malignancies. This study aimed to evaluate the predictive role of two single nuclear polymorphisms (SNPs, rs3826793 and rs2070246) in the ASCT2 gene in HCC patients treated by TACE. Materials and Methods: Two functional SNPs (rs3826793 and rs2070246) in the ASCT2 gene were selected and genotyped using the Sequenom iPLEX genotyping system in a cohort of 448 unresectable Chinese HCC patients treated by TACE. Univariate and multivariate Cox proportional hazards models and Kaplan-Meier curves were used for the prognosis analyses. Results: There was no significant association between two SNPs (rs3826793 and rs2070246) in the ASCT2 gene and overall survival of TACE treated HCC patients. However, we demonstrated that patients with early stage HCC carrying T genotype in rs2070246 showed better OS than those carrying CC genotype (P=0.023). Conclusions: We demonstrated that patients with early stage HCC carrying T genotype in rs2070246 showed better OS than those carrying CC genotype.