• Asian-Australasian Journal of Animal Sciences
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• 제15권5호
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• pp.671-674
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• 2002

This study investigated the use of multinutrient blocks (MNB) composed of 35% date syrup, 35% date syrup byproducts, 10% date fronds, 7% urea, 7% cement and 6% common salt for growing small ruminants, as partial substitute for the roughage component of the diet (Rhodes grass hay) and its effect on feedlot performance and economics of feeding. Eight growing local Omani goats and 8 sheep (each about one year old) were used in this study. Mean body weights for goats and sheep (kg), respectively were $21.1{\pm}4.5$ and $25.5{\pm}4.1$. The goats and sheep were subdivided into two sub-groups of more or less equal body weights for each species. Each sub-group in both species was either fed on 0.5 kg concentrate+ad libitum Rhodes grass hay or the same diet+restricted hay (about 0.2 kg/head/day) and ad libitum amount of MNB. Sheep significantly (p<0.05) consumed greater amounts of MNB ($36{\pm}17$g/head/day) than goats ($6{\pm}2.5$ g/head/day). Feeding of the MNB was effective in sparing about 40% of the roughage Rhodes grass hay for goats (from 240 to 140 g) and about 42% for sheep (from 252 to 146 g) or approximately 100 g/head/day for both goats and sheep. This reduction (or sparing) in the consumption of Rhodes grass hay was coupled by an improvement in daily liveweight gain (g/head/day) in both goats (from 29 to 46 by 58.6%) and sheep (from 26 to 39 by 50%) and also by an improvement in the feed conversion efficiency (g feed/g gain) of both goats (from 25 to 13.8 by 45%) and sheep (from 28.7 to 17.2 by 40%). Cost of daily consumed feeds as well as cost/kg gain (or cost of meat) were both reduced due to feeding of MNB. They were both respectively reduced by 7.5% (from 53 Baisa/day to 49) and 38% (from 1,828 Baisa/kg to 1,140). It was economically viable to feed MNBs containing date by-products and urea to small ruminants in the Sultanate of Oman.

• Preventive Nutrition and Food Science
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• 제9권1호
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• pp.7-13
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• 2004

Lactic acid fermentation of prickly pear extract (PPE) was performed by Lactobacillus rhamnosus LS, Lactobacillus bulgaricus, and Lactobacillus brevis. The PPE was pasteurized to eliminate indigenous microorganisms as well as to dissolve the partially insoluble pulp. The PPE fermented without yeast extract by L. rhamnosus LS exhibited 0.57％ acidity and 3.5${\times}$10$^{8}$ CFU/mL bacteria count. With the addition of 0.2% edible yeast extract the PPE fermented by L. rhamnosus LS exhibited 1.15％ acidity,2.7${\times}$10$^{9}$ CFU/mL bacteria count and 95.0% retention of red color. When 5％ fructose syrup was added, the PPE fermented by L. rhamnosus LS had 1.09% acidity, 6.5${\times}$10$^{8}$ CFU/mL, and 97.7% retention of red color. With 1∼3% (w/v) concentrations of starter, the PPE fermented by L. bulgaricus and L. brevis showed 0.97% and 0.65% acidities, respectively. The viable cell counts from L. rhamnosus LS fermentation were higher compared with those of other LAB. During cold storage at 4$^{\circ}C$, the viable cell count was well maintained for 3 weeks, but then rapidly decreased. The red pigment was highly stable during cold storage for 4 weeks. The pasteurized PPE fortified with 5% fructose syrup, 0.2% yeast extract, and 0.05% CaCO$_3$ was successfully fermented by inoculating with 3% LAB and incubating at 3$0^{\circ}C$ for 2 days. Both viable cell counts and the red color of the fermented PPE were well maintained during cold storage for 3 weeks.

• 대한유전성대사질환학회지
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• 제14권1호
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• pp.1-9
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• 2014

Many inborn errors of metabolism can be completely cured with early detection and early treatment. This is why neonatal screening on inborn errors of metabolism is implemented worldwide. In 1991, the Ministry of Health & Social affairs adopted a nationwide service program for neonatal screening of phenylketonuria, galactosemia, maple syrup urine disease, homocystinuria, histidinemia and congenital hypothyroidism for newborns delivered from low class pregnant women registered in health centers. Government decreased the test items from six to two, PKU and congenital hypothyroidism to increase test numbers with same budget from 1995. 78 laboratories wanted to participate for neonatal screening test in 1999. Government decided to screen six items of PKU, congenital hypothyroidism, maple syrup urine disease, homocystinuria, galactosemia and congenital adrenal hyperplasia from 2006. In 2014, thirteen laboratories are participating. Inter laboratory quality control was started 6 times a year from 1994. In case a patient with an inherited metabolic disease is diagnosed by screening of government program, special milk is provided at government's expense. According to the government project, from 1997 to 2013, 7,080,569 newborns were screened. 144 PKU, 2.451 congenital hypothyroidism were detected. So incidence of PKU is 1/49,170 and congenital hypothyroidism is 1/2,888. The cost benefit of performing screening procedures coupled with treatment has been estimated to be as high as 1.77 times in PKU, 11.11 times in congenital hypothyroidism than cost without screening. By January 2007, many European countries had expanded of their newborn screening programs by inclusion of Tandem mass spectrometry. We are trying to increase the budget to test all newborns for Tandem mass spectrometry from 2016. We are considering four to five central laboratories which cover all newborns and are equipped with tandem mass spectrometer & enzyme immunoassay for TSH, 17OHP & enzyme colorimetric assay for galactose. And I hope to expand test including Wilson disease screening test and lysosomal storage diseases.

• Applied Biological Chemistry
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• 제42권2호
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• pp.152-155
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• 1999

Caramel 반응의 반응속도의 향상을 위하여 pH, 아미노산, 가수분해 단백질 그리고 인산염의 효과를 검토하였다. 반응기질을 물엿으로 하여 $110^{\circ}C$의 온도에서 반응시키면서 Suv(different color functions-metric saturation)와 HMF(5-hydroxymethylfurfural) 함량을 측정하였으며 갈색도는 420 nm에서 흡광도를 측정하였다. 반응 pH를 4에서 10으로 증가시켰을 때 반응속도는 31.9% 향상되었으며 HMF 함량과 420 nm에서의 흡광도도 같이 증가하는 경향을 나타내었다. 여러 아미노산을 첨가한 결과 arginine, glycine과 같은 중성 또는 염기성 아미노산이 caramel반응 속도 향상에 매우 효과적이었으며 이는 Maillard반응이 관여되었기 때문으로 사료된다. 반응 향상 효과가 있는 arginine과 glycine 그리고 가수분해 단백질(HVP; hydrolyzed vegetable protein, HAP; hydrolyzed animal protein)을 $K_2HPO_4$와 여러 비율로 혼합첨가한 결과 $K_2HPO_4$에 의하여 arginine과 HAP는 반응향상 효과가 매우 높아진 반면 glycine과 HVP의 효과는 감소되었다.

• 한국식생활문화학회지
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• 제5권4호
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• pp.431-436
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• 1990

새로운 기능성 식품소재로 그 용도가 확대되고 있는 당유도체 감미료 Fructo-oligo 당, Maltitol, Sorbitol 및 High maltose syrup의 수분 활성도 저하능력, 유산균 발효능력, 난우충성 및 상대감미도에 대하여 조사하였다. 수분활성도 저하능력은 Sorbitol이 다른 당보다 우수하였으며 Fructo-oligo 당, Maltitol은 설탕과 유사하였다. 여러 가지 유산균에 대한 발효능력은 glucose(Hydrol), Fructo-oligo 당, HMS 모두 우수하였으나 Maltitol은 Lactobacillus plantarum을 제외한 다른 유산균들의 생육을 억제하였다. Streptococcus mutans를 이용한 우충성 시험에서 Maltitol은 다른당에 비하여 뚜렷한 난우충성을 나타내었다. 당유도체들의 상대감미도는 당도수준이 높을수록 증가하는 경향을 나타내었으며 1% 설탕용액의 당도수준에서 mole 농도기준 상대감미도는 Neosugar 0.60, Sorbitol 0.21, Maltitol 0.50, HMS 0.27로 평가되었다.

• 한국지역사회생활과학회지
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• 제22권4호
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• pp.573-578
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• 2011

This research was conducted to enhance the value of low quality sweet persimmon by processing paste by combining low quality sweet persimmon and red-bean. In order to make sweet persimmon paste containing red-bean, the following procedures were executed : 1) the sweet persimmon was cleaned by water 2) the fruit stalk was removed 3) it was peeled, sliced, pitted and crushed 4) steamed and peeled red-bean paste was mixed 5) sweetening materials(starch syrup, sugar and dextrin) and salt were mixed 6) boiled and stored in refrigerator. Sweet persimmon was peeled, stem and seed was removed to make the paste. When mixing sweet persimmon paste and red-bean paste in the ratio of 6 : 4, sensory point of taste and overall desirability were higher than mixing in 5 : 5 and 4 : 6 but texture was lower. The sweet persimmon paste containing red-bean was softened and the sugar content was increased from 41.4$^{\circ}$Brix to 53.1$^{\circ}$Brix when 10% dextrin was added. The overall sensory evaluation was higher in sweet persimmon paste containing red-bean than commercial red-bean paste when 10% dextrin was added. The overall desirability also shown a significant difference between them. Even though findings were statistically insignificant, sweet persimmon powder with red-bean paste increased in texture and overall desirability compared with commercial red-beans paste. Sweet persimmon powder paste containing red-bean significantly decreased in flavor, color, taste and overall desirability compared with sweet persimmon paste containing red-bean. As a result of this study, the best combination for the quantity of sub-materials to make sweet persimmon paste was 600g of sweet persimmon, 400g of red-bean, starch syrup 120g, sugar 60g, salt 4g and dextrin 100g.

• 한국식품저장유통학회지
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• 제13권5호
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• pp.581-588
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• 2006

팥앙금과 설탕 및 물엿을 혼합한 모델용액에서 물엿농도 및 가열조건에 따른 팥앙금 호화액의 당 및 아미노산 함량의 동적변화를 모니터링 하였다. 중심합성계획에 의해 유리당 함량을 측정한 결과, 갈변반응이 심하게 발생한 실험구에서 glucose와 fructose의 함량이 높고, maltose의 함량이 낮게 나타났다. 또한 팥앙금 호화액의 amino acid 함량을 분석한 결과, total amino acid 함량은 가열온도에 가장 많은 영향을 받았고, 갈변반응이 많이 일어난 시료에서 그 함량이 크게 증가하였다. 각 시료의 갈색도 측정 결과 glucose 및 아미노산의 변화와 마찬가지로 $95^{\circ}C$의 온도까지는 그 함량이 감소하다가 그 이상의 범위에서는 가열온도가 높아짐에 따라 증가하였다. 팥앙금 호화액의 유리당 및 아미노산 함량과 갈색도와의 상관관계를 조사해 본 결과, 양의 상관관계를 나타내어 갈색도의 증가로 인한 유리당 및 아미노산의 감소보다는 열분해에 의한 유리당 및 아미노산의 증가 속도가 더 빠른 것으로 여겨진다.

• 대한지역사회영양학회지
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• 제11권6호
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• pp.771-778
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• 2006

Previous studies have suggested that delta-aminolevulinic acid dehydratase (ALAD) phenotype differently affect mineral metabolism. The objective of this study was to determine the effectiveness of 6-month iron supplementation as syrup of NaFeEDTA in improvement of iron status according to ALAD genotype. One hundred thirty adult women living in rural areas of Asan were provided NaFeEDTA syrup once a week for 6 months at the dose of 64mg Fe/week. Three hundred control subjects were observed during the study period. Fasting blood was obtained for analyzing hemoglobin (Hb) and zinc protophorphyrin (ZPP) and serum was analyzed for ferritin, iron and total iron capacity (TIBC) levels before and after iron supplementation. Ninety percent of ALAD 1-1 (ALAD1) and 10% of ALAD 1-2 (ALAD2) genotype were observed in the control group. However in the intervention group, 98% showed ALAD1 while only 2% was ALAD2, which is significantly lower proportions of ALAD2 compared to the control group (p<0.01). The iron status of Intervention group significantly improved except for ferritin and TIBC regardless or ALAD genotype, while the control group did not show any changes in iron status except for ZPP. ZPP concentration of the control group significantly increased in both ALAD1 and 2 while the intervention group showed significantly decreased ZPP after supplementation in ALAD1. Iron supplementation in the form of NaFeEDTA seems to be effective in reduction of ZPP levels although ALAD2 did not show significant changes due to the small number. However, it is difficult to make a conclusion from these results, and more specified further investigation is needed with more participants.

• 약학회지
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• 제58권3호
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• pp.190-199
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• 2014

The Korean Pharmaceutical Codex (KPC) analytical method of ambroxol hydrochloride and clenbuterol hydrochloride formulation is complicated and needed to carry out multiple processes during the test. To improve the low efficiency of analytical procedure that makes pharmaceutical laboratory consume much time and high cost to conduct the test of this formulation, this study was performed for simplifying the pretreatment process and optimizing conditions of the HPLC assay. The analytical procedure using HPLC was developed to establish analytical specification for ambroxol hydrochloride and clenbuterol hydrochloride formulations. The newly developed analytical method has good linearity ($R^2$ >0.999), specificity, precision (RSD<1.0%) and the recovery ranges of 98.50~101.84% for ambroxol, 98.29~101.35% for clenbuterol syrup and 98.66~101.71% for clenbuterol tablets. The LOQs were 0.204 ${\mu}g/ml$ for ambroxol, 0.021 ${\mu}g/ml$ for clenbuterol syrup and 0.073 ${\mu}g/ml$ for clenbuterol tablets. The new method was performed with commercially available samples to confirm analytical conditions and validated to be suitable for saving time and cost to control the quality of routine manufactured products. This analytical method will be used for revising the monograph of ambroxol hydrochloride and clenbuterol hydrochloride formulation in next supplement of KPC.

• Natural Product Sciences
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• 제27권2호
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• pp.128-133
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• 2021

The Coumarin (1,2-benzopyrone) is the main secondary metabolite of Mikania laevigata Sch. Beep ex Baker and Mikania glomerata Spreng., which are popularly known as guaco. These plants have been used mainly in traditional medicine in the treatment of respiratory diseases because their bronchodilator effect. However, there are around 200 species of Mikania, which are quite similar in appearance. From these, only M. leavigata and M. glomerata have high concentrations of coumarins. In this line, the falsification of products Mikania based has been frequent. In this sense, this work demonstrated the application of the easy, fast, e not destructive method based in Nuclear Magnetic Resonance in quantitative mode (qNMR) for the determination of coumarin in both commercial and homemade guaco products. Thus, in the first step the compounds were extract from guaco leaves and syrups using chloroform (CHCl₃), with or without ultrasound. About the method, was linear with a R² = 0.9947 for 1,2-benzopyrone, with detection and quantification limits with were 0.11 and 0.36 mg mL^-1 respectively. In the same line, the method was safe with RSD <0.3% and with recovery ranging from 93-101%. To confirm the applicability of the method, in the last step was applied to 10 real samples (6 from leaves and 4 from syrups). The content of the coumarin in the leaf extract ranged from 0.62 to 1.30 mg mL^-1. For syrups I, II and IV, the content of coumarin was in accordance with the manufacturers. However, for de Syrup III, the concentration was 155% higher. In summary, the qNMR is a rapid method with minimal sample preparation that can be used to quantify coumarin in home-made plant extracts as well as in commercial samples as syrup for instance. This method is applicable for quality control of different plants-based products.