• Title/Summary/Keyword: synthetic analysis

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Characterization of Acyl-CoA Oxidases from the Lipolytic Yeast Candida aaseri SH14

  • Ibrahim, Zool Hilmi;Bae, Jung-Hoon;Sung, Bong Hyun;Kim, Mi-Jin;Rashid, Ahmad Hazri Ab;Sohn, Jung-Hoon
    • Journal of Microbiology and Biotechnology
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    • v.32 no.7
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    • pp.949-954
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    • 2022
  • The lipolytic yeast Candida aaseri SH14 contains three Acyl-CoA oxidases (ACOXs) which are encoded by the CaAOX2, CaAOX4, and CaAOX5 genes and catalyze the first reaction in the β-oxidation of fatty acids. Here, the respective functions of the three CaAOX isozymes were studied by growth analysis of mutant strains constructed by a combination of three CaAOX mutations in minimal medium containing fatty acid as the sole carbon source. Substrate specificity of the CaAOX isozymes was analyzed using recombinant C. aaseri SH14 strains overexpressing the respective genes. CaAOX2 isozyme showed substrate specificity toward short- and medium-chain fatty acids (C6-C12), while CaAOX5 isozyme preferred long-chain fatty acid longer than C12. CaAOX4 isozyme revealed a preference for a broad substrate spectrum from C6-C16. Although the substrate specificity of CaAOX2 and CaAOX5 covers medium- and long-chain fatty acids, these two isozymes were insufficient for complete β-oxidation of long-chain fatty acids, and therefore CaAOX4 was indispensable.

The identification of amethyst origins by using FT-IR, UV-VIS spectrum analysis (ET-IR, UV-VIS 분광분석을 통한 자수정의 산지감별)

  • Yoon Si-Nae;Yon Seog-Joo
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.16 no.1
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    • pp.25-31
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    • 2006
  • Many amethysts of different origins such as South America, Aftrica, and Asia were compared. According to ICP-AES analysis, the colorizing elements were Fe, Cr, Cu, and Co. Amethysts from South America showed higher concentrations of the elements. UV- VIS analysis showed that both natural and synthetic amethysts were characterized by the higher absorption at 350 nm and 550 nm wavelength. The natural amethysts had lower permeability than that of the synthetic amethysts. According to FT-IR analysis, the synthetic amethysts were distinguished from the natural amethysts in that they had a typical absorption band of $3543cm^{-1}$. The amethyst from South America showed strong absorption bands of $3584cm^{-1}\;and\;3435cm^{-1}$, and African amethyst had absorption bands of $3299cm^{-1}\;and\;3196cm^{-1}$. $3381cm^{-1}$ absorption band was shown strongly for Asian origins.

A STUDY ON THE PARAMETER ESTIMATION OF SNYDER-TYPE SYNTHETIC UNIT-HYDROGRAPH DEVELOPMENT IN KUM RIVER BASIN

  • Jeong, Sang-man;Park, Seok-Chae;Lee, Joo-Heon
    • Water Engineering Research
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    • v.2 no.4
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    • pp.219-229
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    • 2001
  • Synthetic unit hydrograph equations for rainfall run-off characteristics analysis and estimation of design flood have long and quite frequently been presented, the Snyder and SCS synthetic unit hydrograph. The major inputs to the Snyder and SCS synthetic unit hydrograph are lag time and peak coefficient. In this study, the methods for estimating lag time and peak coefficient for small watersheds proposed by Zhao and McEnroe(1999) were applied to the Kum river basin in Korea. We investigated lag times of relatively small watersheds in the Kum river basin in Korea. For this investigation the recent rainfall and stream flow data for 10 relatively small watersheds with drainage areas ranging from 134 to 902 square kilometers were gathered and used. 250 flood flow events were identified along the way, and the lag time for the flood events was determined by using the rainfall and stream flow data. Lag time is closely related with the basin characteristics of a given drainage area such as channel length, channel slope, and drainage area. A regression analysis was conducted to relate lag time to the watershed characteristics. The resulting regression model is as shown below: ※ see full text (equations) In the model, Tlag is the lag time in hours, Lc is the length of the main river in kilometers and Se is the equivalent channel slope of the main channel. The coefficient of determinations (r$^2$)expressed in the regression equation is 0.846. The peak coefficient is not correlated significantly with any of the watershed characteristics. We recommend a peak coefficient of 0.60 as input to the Snyder unit-hydrograph model for the ungauged Kum river watersheds

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A Study on the Subjective Evaluation and Physical Properties of Natural/Artificial Rabbit Hairs (천연 인조 토끼털의 주관적 평가 및 물리적 성질에 관한 연구)

  • Lee, Seon Ah;Kim, Jongjun
    • Journal of Fashion Business
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    • v.21 no.4
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    • pp.144-158
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    • 2017
  • Fur garment has long been the conventional symbol for luxury, or conspicuous consumption. However, as fashion items began to diversify as part of overall fashion trend, fur items are now more about individual taste and style than just lavishness. Synthetic fur is especially emerging as a new promising fashion material, with a touch almost like natural fur at an affordable price. Along with the emergence of 'Vegan Fashion' trend, synthetic fur is establishing itself as a popular fashion textile. This study is an attempt to investigate subjective evaluation and physical properties of natural and synthetic furs, whose results will further serve as basic data in developing synthetic fur materials. Sensory and emotional evaluations are carried out on natural and artificial furs. For analysis, factors such as weight, thickness, air permeability, gloss and compressibility were surveyed to observe how they influence the physical properties. According to the subjective evaluation, natural and artificial fur samples do not differ in conspicuous ways in appearance. Experiments on physical properties, specifically warm/cool touch experiment, show that natural fur has a slightly higher warm sensation than artificial fur. Luster analysis by using a microscope revealed that there are subtle qualitative differences between natural and artificial fur. During the subjective evaluation, subjects found it hard to state distinct quantitative differences in luster. A survey as a means of assessing qualitative differences in gloss seems to be necessary to complement the evaluation. Results from this study will potentially serve as resources for diversification of fashion product designs using synthetic fur.

The Development of Synthetic Unit Hydrograph Suitable to the Hydrologic Characteristics in Korea (국내 수문특성에 적합한 합성단위도의 개발)

  • Jeong, Seong-Won;Mun, Jang-Won
    • Journal of Korea Water Resources Association
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    • v.34 no.6
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    • pp.627-640
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    • 2001
  • Generally, the synthetic unit hydrograph method is presented to estimate the design flood in the ungaged watershed. However, due to the lack of rainfall-runoff data, the models developed in other countries such as U.S.A. and Japan have been widely used in Korea. Therefore, it may be essential to develope the rainfall-runoff model suitable for the hydrological char-acteristics in Korea. In this study, the representative unit hydrographs are derived from rainfall-runoff data at 19 basins in Selma-Cheon and 3-IHP experimental watersheds using ridge-regression method and Nash model. And a new synthetic unit hydrograph for Korea is suggested by integrating the described results and previous studies on unit hydrograph. The newly developed method is represented as two regression forms with three independent variables of watershed area, channel length, and channel slope by multiple regression analysis is carried out for each watershed, the coefficients of determination are not improved in all cases compared out for each watershed, the coefficients of determination are not improved n all cased the synthetic unit hydrograph for each watershed. Therefore, when the new method is applied to some watersheds, the result analyzed for all data has to be used.

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A New Method for Measuring Fiber Length and Fiber Coarseness Using Image Analysis Technique (화상분석법을 응용한 섬유장 및 섬유 조도 측정법 개발)

  • 배진한;김철환;박종열
    • Journal of Korea Technical Association of The Pulp and Paper Industry
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    • v.34 no.2
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    • pp.13-21
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    • 2002
  • A new method for measuring fiber length and fiber coarseness was developed using image analysis technique. Measured fibers were transferred to a glass slide on a filter paper placed on a wire of the laboratory paper machine. After staining the fibers on the slide, mean fiber lengths and coarseness were measured by a commercial image analysis software, named KS400. The resultant data obtained from the image analysis displayed a close correlation with those from FS-200 and also showed excellent reproducibility as well as those from FS-200. The length of synthetic fibers over 10 mm long could be readily measured by this new analysis technique. Finally, a substantial improvement in precision for measuring fiber length and coarseness was made with less operator's effort for a given time.

Plant Protein Improvement by Synthetic Gene (합성유전자를 이용한 식물단백질의 향상)

  • 김태금;양문식
    • KSBB Journal
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    • v.7 no.3
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    • pp.155-160
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    • 1992
  • To improve the nutritional quality of plant proteins, a synthetic gene, called HEAAE (high essential amino acid encoding)-DNA, was introduced and expressed in tobacco plants. The synthetic gene, which is 292 basepair-long, codes for a protein composed of about 80% essential amino acids. To improve its expression level in plants, Cauliflower Mosaic Virus (CaMV) 355 and CaMV duplicate 35S promoters which are known as strong promoters were used with Nopaline Synthase promoter as a control. Transformed and regenerated tobacco plants were subject to analysis for introduction and expression of this gene. Integration of the gene into the plant genome and its expression into mRNAs and its proteins have been demonstrated using Southern, northern blot analysis and amino acid analysis. The differences of expression levels among CaMV duplicate 35S, CaMV 35S and Nopaline Synthase promoters are significant in term of mRNAs, but not in terms of proteins.

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WAVENUMBER CORRELATION ANALYSIS OF RADAR INTERFEROGRAM

  • Won, Joong-Sun;Kim, Jeong-Woo
    • Proceedings of the KSRS Conference
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    • 1999.11a
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    • pp.425-428
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    • 1999
  • The radar interferogram represents phase differences between the two synthetic aperture radar observations acquired in slightly different angle. The success of the radar interferometric application largely depends on the quality of the interferogram generated from two or more synthetic aperture radar data sets. We propose here to apply the wavenumber correlation analysis to the in-phase and quadrature phase of the radar interferogram. The wavenumber correlation analysis is to resolve the highly correlated components from the low correlation components by estimating correlation coefficients for each wavenumber component. Through this approach, one can easily distinguish the signal components from the noise components in the wavenumber domain. Therefore, the wavenumber correlation analysis of the radar interferogram can be utilized to design post filter and to estimate the quality of interferogram. We have tested the wavenumber correlation analysis using a Radarsat SAR data pair to demonstrated the effectiveness of

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Cost Analysis of High-Calorie Synthetic Natural Gas (고열량 합성천연가스 제조원가 분석)

  • Kim, Hyungsik;Park, Jinmo;Han, Jaryoung;Lee, Youngchul
    • Journal of the Korean Institute of Gas
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    • v.22 no.2
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    • pp.67-71
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    • 2018
  • The calorific value of general synthetic natural gas(SNG) mainly composed of methane is $9,300kcal/m^3$ or less. In order to use such synthetic natural gas as city gas in Korea, it is necessary to heat up to $10,200kcal/m^3$. Generally, propane gas is commonly used to control the calorific value of SNG. However, the price of propane gas has fluctuated widely and has been higher than the cost of producing SNG. So if the high-calorie SNG can be produced directly instead of the calorie control by propane mixing, the production price of SNG can be significantly reduced. In this paper, based on the US NETL analysis, we compare the cost of methane based SNG production and the cost of high-calorie SNG direct production.

Identification and Removal of Pigments in Blood-colored Grooves of Long Sword, Admiral Yi Sun-sin (Treasure No. 326) (보물 제326호 이순신 장검 혈조 내 안료의 규명 및 제거)

  • Kwon, Hyuk Nam;Youn, Hye Seong;Ryu, Dong Wan;Lee, Jeong Won;Lee, Jang Jon;Han, Min Soo
    • Journal of Conservation Science
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    • v.31 no.4
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    • pp.499-506
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    • 2015
  • The artifacts of Admiral Yi Sun-sin (Treasure No.326) consist of six items with two long swords, a jade decoration, a belt and two peach-shaped cups. In commemoration of the opening of Chungmugong Yi sun-sin Memorial Museum (April 28, 2011), there were conservation treatments for those artifacts at the beginning of 2011. At that time, from the result of non-destructive surface analysis on pigments in blood-colored grooves of the two long swords, it was presumed to be synthetic resin paints. This study shows the accurate identification on pigments which was presumed as synthetic resin paints and its removal. To identify pigments in blood-colored grooves, the optical microscope, scanning electron microscope, Micro-XRF, XRD and FT-IR were used for the analysis. The results turned out that the thickness of $10{\sim}90{\mu}m$ in a single layer was measured. And major components containing Pb and Cr and lead chromium molybdenum oxide were identified and to identify components of adhesives from the analysis by FT-IR, it turned out to be alkyd resin, which can be presumed that they were recently colored with synthetic paints. The synthetic paints easily react with most of the organic solvents. And it is so easy to handle without the effect on metal that it was removed with acetone. While removing synthetic paints, all of paints on it were collected for identifying whether there is traditional pigment but nothing was identified.