• Biomedical Science Letters
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• v.17 no.2
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• pp.167-171
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• 2011

[ ${\alpha}$ ]synuclein (${\alpha}$-syn) is implicated in the pathogenesis of Parkinson's disease (PD) and other related diseases. We have previously reported that ${\alpha}$-syn binds to the cell membranes in a transient and reversible manner. However, little is known about the physiologic function and/or consequence of this association. Here, we examined whether chemically induced perturbations to the cellular membranes enhance the binding of ${\alpha}$-syn, based on hypothesis that ${\alpha}$-syn may play a role in maintenance of membrane integrity or repair. We induced membrane perturbations or alterations in ${\alpha}$-syn-overexpressing human neuroblastoma cells (SH-SY5Y) by treating the cells with hydrogen peroxide ($H_2O_2$) or oleic acid. In addition, membranes fractionated from these cells were perturbed by treating them with proteinase K or chloroform. Dynamic interaction of ${\alpha}$-syn to the membranes was analyzed by the chemical cross-linking assay that we developed in the previous study. We found that membrane interaction of ${\alpha}$-syn was increased upon treatment with membrane-perturbing reagents in a dose and time dependent manner. These results suggest that perturbations in the cellular membranes cause increased binding of ${\alpha}$-syn, and this may have significant implication in the physiological function of ${\alpha}$-syn in cells.

• Biomedical Science Letters
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• v.18 no.4
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• pp.329-337
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• 2012

${\alpha}$-synuclein (${\alpha}$-syn) is known to be implicated in the pathogenesis of Parkinson's disease and transiently bind to biological vesicles. In this study, we examined the effect of molecular crowding on the interaction of ${\alpha}$-syn with biological vesicles by using inert polymers since the environment of proteins in cells are crowded with other macromolecules. The addition of different polymers including polyethylene glycol, dextran, and ficoll enhanced the binding of ${\alpha}$-syn to vesicles in a concentration-dependent manner and the association of ${\alpha}$-syn with vesicle was proportionally augmented by increased expression of ${\alpha}$-syn. However, molecular crowding had a neglectable effect on the vesicle binding of ${\alpha}$-syn mutants (A30P, TG6), which has been reported to show impaired vesicle binding capacity. These results suggest that transient interaction of ${\alpha}$-syn with vesicles occurs more commonly in cells than expected implying interaction with vesicles may be one of the physiological processes in which ${\alpha}$-syn is involved.

• Bulletin of the Korean Chemical Society
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• v.16 no.1
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• pp.12-16
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• 1995

The diastereoselectivity of addition reaction of crotylmetal reagents to cobalt-complexed acetylenic aldehydes and metal-free aldehydes was examined. The anti-diastereomer was the predominant product when the crotyl metallics were Cr, Sn, and Zr. In THF, the uncomplexed aldehydes normally gave higher anti-diastereoselectivity. However, the cobalt-complex of silicon-substituted propynals with three bulky substituents produced increased proportions of syn-diastereomer. In DMF, the selectivity shifted towards syn-isomer except in the case of dimethylphenylsilyl substituent. When tributylstannane was used in the presence of BF3 etherate, moderate syn- selectivity was observed with uncomplexed aldehydes, but only decomposed products from complexed aldehydes.

• Journal of the Korean Society of Safety
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• v.17 no.2
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• pp.32-38
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• 2002

In this paper, a new approach for the SynRM(Synchronous Reluctance Motor) control which ensures producing MTPA(Maximum Torque per Ampere) over the entire field weakening region is presented. In addition, this paper presents a speed sensorless control scheme of SynRM using flux observer. Also, by adjusting the base speed for the field weakening operation according to the flux level, the current and voltage limit, the smooth and precise transition into the field weakening operation can be achieved. The validity of the proposed scheme is verified through simulation.

• The Transactions of the Korean Institute of Electrical Engineers P
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• v.54 no.4
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• pp.185-191
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• 2005

In this paper, a new approach for the Synchronous Reluctance Motor control which ensures producing Maximum Torque per Ampere(MTPA) over the entire field weakening region is presented. In addition, This paper presents a speed sensorless control scheme of SynRM using artificial neural network. Also, by adjusting the base speed for the field weakening operation according to the flux level, the current and voltage limit, the smooth and precise transition into the field weakening operation can be achieved. The proposed scheme is verified validity through simulation.

• Journal of Photoscience
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• v.12 no.3
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• pp.185-186
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• 2005

The electronic structure of photoskinsensitizing psoralens has been investigated by the ab initio calculations. The photocycloaddition reaction of 8-methoxypsoralen with thymine is studied as a model for the photosensitizing reaction of psoralen with DNA bases. The photocycloadduct was inferred to be a C4-cycloaddition product with the stereochemistry of Syn, H-H, Syn, H-T formed through [2+2] addition reaction between the 3,4-double bonds of 8-methoxypsoralen and 5,6-double bond of thymine base.

• Proceedings of the Korean Institute of IIIuminating and Electrical Installation Engineers Conference
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• 2007.05a
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• pp.311-315
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• 2007

In this paper, a new approach for the Synchronous Reluctance Motor control which ensures producing Maximum Torque per Ampere(MTPA) over the entire field weakening region is presented. In addition, This paper presents a speed sensorless control scheme of SynRM using artificial neural network. Also, by adjusting the base speed for the field weakening operation according to the flux level, the current and voltage limit, the smooth and precise transition into the field weakening operation can be achieved The proposed scheme is verified validity through simulation.

• Journal of the Korean Ceramic Society
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• v.38 no.2
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• pp.238-238
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• 2001

15% Syn M, sintered mullite(SM) and fused mullite(FM) was added to produce high alumina refractory materials. Mullite content of SM, FM and Syn M was 93.17%, 86.79%, 97.57% repectively. Mechanical properties of specimens (added 15wt% mullite) ware investigated to be flexural strength 338.60MPa, compressive stcngth 9,427kgf/㎠. But the specimen which imported mullite was added showed 5~10%l lower stregth than syn M added.

• Bulletin of the Korean Chemical Society
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• v.23 no.9
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• pp.1325-1327
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• 2002

• Proceedings of the KIEE Conference
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• 2005.07b
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• pp.1456-1458
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• 2005

In this paper, a new approach for the synchronous reluctance motor control which ensures producing maximum torque per ampere(MIPA) over the entire field weakening region is presented. In addition, This paper presents a speed sensorless control scheme of SynRM using artificial neural network. Also, by adjusting the base speed for the field weakening operation according to the flux level, the current and voltage limit, the smooth and precise transition into the field weakening operation can be achieved. The proposed scheme is verified validity through simulation.