• Journal of Life Science
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• v.30 no.7
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• pp.614-624
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• 2020

The recent development of next-generation sequencing technology has enabled increased genomic analysis, but very few single nucleotide polymorphism (SNP) markers applicable to sweet persimmon (Diospyros kaki Thunb.) cultivars have been identified. In this study, SNP primers developed from five pollination-constant astringent (PCA) persimmons native to Korea were applied to discriminate between cultivars and verify their usability. The polymerase chain reactions of 19 SNP primers developed by Jung et al. were checked, with 11 primers finally selected. The other eight were very difficult to analyze in the agarose gel electrophoresis and QIAxcel Advanced System used in this experiment and were therefore excluded. The 11 SNP primers were applied through first and second verification to 76 cultivars and collection lines including 20 pollination-variant non-astringent (PVNA), 30 pollination-constant non-astringent (PCNA), 20 PCA, and six pollination-variant astringent (PVA). Of these, 38 were indistinguishable (eight PVNA, 18 PCNA, nine PCA, and three PVA). However, the results of applying the 11 SNP primers to new sweet persimmon cultivars, namely Gamnuri, Dannuri, Hongchoo, Jamisi, and Migamjosaeng, showed that they have the potential to be used as a unique marker for simultaneously determining between them.

• Journal of the Korean Society of Food Science and Nutrition
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• v.40 no.9
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• pp.1347-1352
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• 2011

Steak sauces containing Korean persimmon were prepared and characteristics were evaluated. SOP (steak sauce containing soft persimmon), SOPP (steak sauce containing soft persimmon powder), and SWP (steak sauce containing sweet persimmon extract) with 5, 10, and 15% persimmon weight per sauce weight were prepared by a common formula. Antioxidant activity was determined by DPPH and ABTS radical scavenging activity, and sweetness of the sauces increased with increasing persimmon content. DPPH radical scavenging activities for SWP with persimmon content of 5 and 15% were $75.28{\pm}0.03%$ and $81.04{\pm}0.01%$, respectively. ABTS radical scavenging activities of SWP with persimmon content of 5 and 15% were $55.64{\pm}0.06$ and $59.18{\pm}0.07%$, respectively. With addition of persimmon, yellowness (b) and redness (a) increased. In overall acceptance of sensory evaluation, 5% SWP showed a relatively high score.

• Journal of Environmental Science International
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• v.32 no.5
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• pp.365-374
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• 2023

Eight years (2014-2021) of climate data were collected from an automatic weather observation system installed at the foot of Mt. Geumo in Chilgok, Gyeongbuk. Using these data, we investigated local bio-climatological indices (warmth index, WI; coldness index, CI; and effective accumulated temperature, EAT) of the mountain region adjacent to the Keimyung Dongyeong forest. The study area's WI and CI were 109.3℃ and -11.3℃ per month, respectively, averaged across 8 years. These values are indicative of an evergreen broad-leaved forest in the warm temperate climate zone, suitable for cultivating sweet persimmons and figs. Additionally, EAT in Dongyeong was 2,113.7℃, averaged across 8 years, suitable for growing crops such as corn, soybean, and potato.

• Korean Journal of Soil Science and Fertilizer
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• v.49 no.5
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• pp.577-583
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• 2016

Small-sized persimmons produced by high crop load are better accepted in the export markets. However, maintaining high crop load frequently results in weakness of tree vigor, deterioration of fruit quality, and increase of the risks for alternate bearing. This experiment was conducted to determine the combined effects of fertilization rate and leaf-fruit (L/F) ratio on container-grown 3-year-old 'Fuyu' persimmon trees. Application of 3.6-g N, 2.1-g $P_2O_5$, 2.7-g $K_2O$, 2.7-g CaO, and 0.6-g MgO was for the control fertilization rate (CF) and that of a 3-fold CF was for the high fertilization rate (HF). Commercial fertilizers were surface-applied to a container on July 6, July 17, and August 10 in three equal aliquots. Single tree for each fertilization rate was assigned for 12 L/F ratios (5, 6.3, 7.7, 9, 10.4, 13, 15.5, 18, 21, 24, 27, and 33) mostly by fruit thinning or rarely by defoliation on July 1. HF did not affect the yield, weight and soluble solids of the fruits but decreased skin color. As L/F ratio increased, yield decreased but average weight, skin color, and soluble solids of fruits increased. With HF, N and K concentrations in leaves, fruits, and shoots increased to some extent but soluble sugars in dormant shoots decreased. Many shoots were cold-injured with low L/F ratio especially at the HF. HF did not increase number of flower buds the next spring either on a shoot or on a tree basis but increased shoot length, compared with the CF. Increasing L/F ratio markedly increased number of flower buds and shoot growth the following year at both fertilization rates. Therefore, an appropriate combination of fertilization rate and L/F ratio should be necessary to maintain stable fruit production and tree vigor at high crop load.

• Journal of Life Science
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• v.30 no.3
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• pp.239-249
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• 2020

Persimmon flowers are fruit-bearing female, pollen-bearing male, or hermaphrodite, containing both a pistil and a stamen. Using prominent PCNA persimmons as male parents is very important for breeding programs, as the selection procedure for new cultivars bearing male flowers requires a long time and a large field in a traditional crossbreeding method. To improve breeding efficiency through early selection of male flower-bearing plants at the seedling stage, analysis was performed on 88 major cultivars whose gender expressions are known, using two male flower selection markers recommended by Akagi et al. The OGI locus marker and DISx-AF4S marker results showed that 83 and 72 cultivars, respectively, matched in terms of gender expression and marker analysis. For the OGI locus marker, 890 plants were selected from 2,509 seedlings obtained from crossbreeding with the mother plant "Migamjosang," which was the breeding cultivar. Comparing the gender expression of the flowers and the marker with 1,186 crossbred seedlings, excluding the unfertilized and dead plants, inconsistencies were found in 401 plants (33.8%). For the DISx-AF4S marker, 636 plants were selected from 889 seedlings obtained from 12 cross-combinations. The results of the sex expression and marker analysis were compared to 379 plants, excluding the unfertilized and dead plants, and inconsistencies were found in 247 plants (65.2%). These results indicate that the examined DISx-AF4S and OGI locus markers would not be suitable for utilization in the breeding field.

• Applied Biological Chemistry
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• v.45 no.2
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• pp.66-70
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• 2002

In this study, three kinds of Saccharomyces cerevisiae strains (KCTC 7106, 7238, 7904) were tested with respect to the ability of alcohol fermentation at $20^{\circ}C$ for 3 weeks, and S. cerevisiae KCTC 7106 was the most effective strain in alcohol fermentation with sweet persimmon. To increase the utility of sweet persimmon, the fruits stored for 1 year were used to make an alcohol beverage. It was adjusted 25% of total sugar content and allowed alcohol fermentation with shaking at 120 rpm and $30^{\circ}C$. The alcohol content of 11.6% showed at 15 days and it did not change until 25 days. The initial pH of the fermentation was near 5 then decreased to 4 within 5 days and the initial vitamin C content, 5.04 ${\mu}g/ml$, gradually decreased with fermentation periods. In the other study, the alcohol fermentation from fresh harvested persimmons showed 13% alcohol content at $20^{\circ}C$ for 30 days without agitation, while the alcohol content of the fermentation at $30^{\circ}C$ was 11.8% at 25 days and 13.4% at 35 days.

• Korean Journal of Food Science and Technology
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• v.32 no.6
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• pp.1278-1284
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• 2000

The study was performed to elucidate the effects of ethylene-absorbent on the quality of 'Fuyu' persimmon fruits in the MA package. Five persimmons were packed in a MA package film (low density polyethylene, 0.055 mm film thickness), and stored at $-0.5^{\circ}C$ for 60 days. Two persimmons were repacked in a MA package with or without ethylene absorbent $(1\;M\;KMnO_4+zeolite)$ and stored at $-0.5^{\circ}C$. Ten days later, these packages was moved to $2^{\circ}C$ or $25^{\circ}C$ storage room to examine the effect of the ethylene-absorbent on the quality of the fruits. Ethylene removal by enclosed ethylene absorbent in MA packaging reduced the rate of fruit respiration at $25^{\circ}C$, so that $O_2$ and $CO_2$ concentration in packing were maintained higher and lower, respectively, compared to control. These effects were not observed, however, in $2^{\circ}C$ post-storage. Fruit firmness and sugar composition were also influenced by ethylene absorbent, showing more delayed flesh softening and higher sucrose concentration in ethylene absorbent treated fruits than control. But ethylene-absorbent treatment lowered glucose and fructose concentration. That shows that ethylene could influence on sugar composition by inhibiting sucrose inversion to glucose and fructose. The production of ethanol and acetaldehyde was reduced by ethylene removal, but the effect was not so high as other quality indices.

• Journal of the Korean Society of Food Science and Nutrition
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• v.5 no.1
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• pp.11-17
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• 1976

Put four different kinds of Jangdongsi, Pasi, Gamsi, and Taebongsi etc. into glass bottle(Fig. 1) of 1150ml and after modulated constitution of air girding this, by means of $CO_2$ the keep in cold storage on $0{\sim}5^{\circ}C$. The results are as follows : 1. $CO_2$ non-addition group was not changed it's own color to four months regardless of kind of persimmons 2. $CO_2$ added test group by double quantity of container was not more rapidly discoloration. 3. One - half quantity of test group added double, same, one-half and one of fifth quantity of container was the most effect, $CO_2$ as the same kind in S. T. P. 4. Taebongsi of test group of four different kinds added one-half quantity of $CO_2$ was maintained effectually possible it's natural color till seven months. 5. In taste, non-addition of $CO_2$ existed astringency taste but addition of $CO_2$ showed sweet taste like a Gamsi since two months of storage.

• Korean Journal of Organic Agriculture
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• v.30 no.3
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• pp.375-391
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• 2022

This study investigated the online transaction status of low-carbon certified fresh agricultural products and analyzed the low-carbon certified premium. For two months from March to April 2022, eight products (rice, apple, pear, sweet persimmons, paprika, tomatoes, cherry tomatoes, and lettuce) were surveyed at major online shopping malls. The low-carbon certification premium was analyzed using hedonic price analysis model. As a result of the online market survey, the low-carbon certified agricultural products were not traded in the case of rice, cherry tomatoes, and paprika. And the proportion of low-carbon certified agricultural products in the case of tomatoes and lettuce was low. As a result of the low-carbon premium analysis, of the five products that analyzed the low-carbon certification premium, four products excluding pear did not have a low-carbon certification premium. Thus, it is necessary to expand the sale of the low-carbon certified agricultural products by distributors, and various efforts to secure the premium of certified agricultural products are important.

• Korean Journal of Organic Agriculture
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• v.25 no.2
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• pp.445-460
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• 2017

This study was carried out for three years from 2013 to 2015 to investigate the actual condition of pest control of domestic persimmon organically cultivated farmers, to select environment - friendly pest control materials for major pests, and to conduct field test of established control system. The main cultivated cultivar of the reader farmer of organic cultivation was "Buyou" and sprayed organic agricultural material 9~17 times per year for pest control. The most harmful pests were anthracnose, bugs, persimmon fruit worm, and scales, etc. The control materials used were organic lime sulfur mixture, red clay sulfur, emulsifier, bordeaux solution, and self-made plant extracts using garlic, ginkgo nut and pine tree. The effective materials for controlling anthrax were lime sulfur mixture and red clay sulfur. Garlic oil + citronella emulsion, shrubby sophora seed extract + tea extract was effective to control Riptortus clavatus and Euproctis subflava. When installed 60 ea per 10a of mating disruption trap in a sweet orchard, the fruit setting rate was improved by 30%. The results of field test of control system to control 10 times a year in organically grown persimmons were able to harvest fruit of 70.7%. However, since any kinds of pesticides can not be sprayed during the harvest season in September and October, the damaged by bugs did not decreased, and further research is needed.