• Journal of Animal Reproduction and Biotechnology
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• v.39 no.1
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• pp.19-30
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• 2024

Background: Although an understanding of the proliferation and differentiation of fish female germline stem cells (GSCs) is very important, an appropriate threedimensional (3D) research model to study them is not well established. As a part of the development of stable 3D culture system for fish female GSCs, we conducted this study to establish a 3D aggregate culture system of ovarian cells in marine medaka, Oryzias dancena. Methods: Ovarian cells were separated by Percoll density gradient centrifugation and two different cell populations were cultured in suspension to form ovarian cell aggregates to find suitable cell populations for its formation. Ovarian cell aggregates formed from different cell populations were evaluated by histology and gene expression analyses. To evaluate the media supplements, ovarian cell aggregate culture was performed under different media conditions, and the morphology, viability, size, gene expression, histology, and E2 secretion of ovarian cell aggregates were analyzed. Results: Ovarian cell aggregates were able to be formed well under specific culture conditions that used ultra-low attachment 96 well plate, complete mESM2, and the cell populations from top to 50% layers after separation of ovarian cells. Moreover, they were able to maintain minimal ovarian function such as germ cell maintenance and E2 synthesis for a short period. Conclusions: We established basic conditions for the culture of O. dancena ovarian cell aggregates. Additional efforts will be required to further optimize the culture conditions so that the ovarian cell aggregates can retain the improved ovarian functions for a longer period of time.

• Journal of Legislation Research
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• no.44
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• pp.343-386
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• 2013

"Financial ADR" system in South Korea can be represented by so-called "Financial Dispute Resolution System", in which Financial Supervisory Service (FSS) and Financial Dispute Resolution Committee are the principal actors in operation of the system, and this is discussed as an "Administrative Financial ADR System". The system has over 10-year history since it was introduced in around 1999. Nonetheless, it was not until when financial consumer protection began to be highlighted after the 2008 financial crisis that Financial ADR system actually started to draw attention in Korea. This was because interest has been rising in "Alternative Dispute Resolution (ADR)" as an institutional measure to protect financial consumers damaged via financial transactions. However, the current discussion on the domestic Financial ADR system shows an aspect that it is confined to who is to be a principal actor for the operation of Financial ADR institution with main regards to reorganization of supervisory system. This article aims to embody these facts in an institutional model by recognizing them as a problem and analyzing the features of the Financial ADR system, thereby clarifying problems of the system and presenting the direction of improvement. The Korean Financial ADR system can be judged as "administrative model integrated model consensual model quasi-judicial model non-prepositive Internal Dispute Resolution (IDR) model". However, at the same time, it is confronted with a task to overcome the two problems; the system is not equipped with institutional basis for securing its validity in spite of the adopted quasi-judicial effect model; and a burden of operating an integrated ADR system is considerable. From this perspective, the article suggests improvement plans for security of validity in the current system and for expansion of industry-control ADR system, in particular, a system of prepositive IDR model. Amongst them, it suggests further plans for securing the validity of the system as follows; promotion to expand the number of internal persons and to differentiate mediation procedures and effect; a plan to keep a financial institution from filing a lawsuit before an agreement recommendation or a mediation proposal is advised; and a plan to grant suspension of extinctive prescription as well as that of procedures of the lawsuit.

• Clean Technology
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• v.21 no.3
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• pp.164-170
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• 2015

In this study, polysulfone-Escherichia coli biomass composite fiber (PSBF) was prepared by spinning the suspension of PS and E. coli biomass and amine-methylated PSBF (AM-PSBF) was fabricated through the methylation of amine groups in PSBF. As comparing the adsorption characteristics of basic dye, Basic Blue 3 (BB3) by the PSBF and AM-PSBF, the effect of the methylation of amine groups on BB3 adsorption was confirmed. pH edge experiments showed that the BB3 uptake of PSBF and AM-PSBF increased as pH was increased and the BB3 uptake of AM-PSBF was higher than that of PSBF at the same pH. Both of PSBF and AM-PSBF was reached at equilibrium within 5 h and kinetic experimental data were well fitted by the pseudo-first-order kinetic model. By the Langmuir model, the maximum adsorption capacities of PSBF and AM-PSBF at pH 8 were evaluated to be 28.9 and 20.7 mg/g, respectively. The maximum adsorption capacity of AM-PSBF was enhanced 1.4 times comparing that of PSBF. These results indicate that the methylation of amine groups in PSBF leads to the improvement of BB3 adsorption capacity. In addition, the results of desorption experiments revealed that AM-PSBF was repeatedly reusable.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.36 no.2
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• pp.73-88
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• 2000

A series of analysis and experiment on the fishing gear and method of the existing midwater pair trawl net with 12,800mm of maximum mesh size is carried out to determine the most effective shape factor. As the first step of this study, the fishing gear and method for the existing net are analyzed to solve problems, such as being low of the net height by increasing the towing speed, twist of net pendant and twine, suspension of float between knots of mesh. etc. Standing on these results, the existing net are modified by very effective shape based on the theory and technology designing the fishing gear. Also, the model experiment is carried out to determine the most effective shape factor of the modified net in the circulation water channel. The obtained results can be summarized as follows : 1. On the length of net pendants(No.2 and No.3) connected with side line, opening efficiency of net with length 8.0cm of net pendant is the greatest and in the order of 11.5cm, 15.0cm. 2. On the number of the net pendant, opening efficiency of net with 4 lines of net pendant is the greatest and in the order of 3 lines, 2 lines. Center part of side panel is pushed out backward, and the pushed length is increasing by increasing flow speed. 3. On the sinking force, opening efficiency of the net is increasing by increasing sinking force. And opening efficiency shows almost the same when sinking force is greater about 1.5 times than that of the existing net in case of the net with 3 lines of net pendant, and about 1.6 ~ 1.7 times than that of the existing net in case of the net with 2 lines.

• Journal of Applied Biological Chemistry
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• v.54 no.4
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• pp.296-302
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• 2011

Insect-resistant transgenic rice was developed by inserting the mCry1Ac1 a modified gene from the soil bacterium Bacillus thuringiensis (Bt). For biosafety assessment, we studied the effects on survival of cantor Daphnia magna, a commonly used as a model organism in ecotoxicological studies. D. magna fed on Bt rice and its near non-genetically modified (GM) counterparts (Nakdong) grown in the same environment (100% ground rice suspension). The Bt rice was comfirmed to have the insertion of T-DNA and protein expression by the polymerase chain reaction and ELISA analysis. Feeding study showed similar cumulative immobility and abnormal response of D. magna between Bt rice and non-GM counterparts. 48 h-$EC_{50}$ values of Bt rice and non-GM rice showed 4,429 and 2,889 mg/L respectively. The rice no observed effect concentration (NOEC) values for D. magna was suggested 1,000 mg/L. We conclude that the tested Bt-rice and Nakdong similar cumulative immobility for D. magna the widely used model organism. We found out that there is strong possibility that the growth of Bt rice didn't affect to non-target insects.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.3 no.3
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• pp.1-7
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• 1983

In spite of extensive knowledge of the surface chemistry and the transport mechanism in filtration systems, there is still insufficient understanding of the physical characteristics of suspensions and the system components. Because of this, no filtration mechanisms are mathematically generalized to the full extent. The purpose of this paper is to propose experimental equations for the filtration process. using the tracer study in filter layer. Some of results are as follows. (1) The Volume of the specific deposit (${\sigma}$) in filtration was directly measurable using the tracer study without interrupting the filtration. (2) It was also confirmed that the head loss in filtration was greatly in fluenced by the micro-air babbles. (3) The correction coefficient(f) was introduced into the Kozeny-Carman equation in order to apply it for the clogging filter media. The coefficient(f) was experimentally obtained. The total head loss of the filter media is given by next equation. $${\frac{h}{h_0}}={\frac{1}{L}}{\int}^{z=L}_{z=0}f({\sigma})g({\varepsilon}_0,{\sigma})dz$$ $$f=aexp(-b{\sigma})$$ The above equation was applicable without regard to the variation of the suspension concentration, the filter medium diameter, the filter depth, the filtration velocity, and the amount of aluminum in all continuous filtration experiments. (4) The total head loss was graphically generalized assuming mathematical filtration models I II (see fig. 7,8) (5) The total head loss was obtained from the filtration model in the field filtration conditions. (see fig. 9,10)

• Korean journal of applied entomology
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• v.56 no.4
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• pp.403-412
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• 2017

To investigate the control effect of insect pests by natural enemies, sticky traps were used in commercial tomato greenhouses in Chun-cheon and the experiment fields of Gangwon-do Agricultural Research and Extension Services, Republic of Korea using semi-forcing culture (February to June). We selected low toxicity pesticides, environment-friendly agricultural materials (EFAM), and natural enemies of the study species, combinations of which have been previously used in farms to control insect pests. In this study, Trialeurodes vaporariorum and thrips, which are major agricultural insect pests, were studied in experimental greenhouses. The adult T. vaporariorum population was observed in mid-April and the population of thrips showed occurrences in early April. Regarding seasonal fluctuation, T. vaporariorum peaked in mid-May and thrips peaked after June. one insecticide, spiromesifen suspension concentrate (SC); one fungicide, cyazofamid SC; and two EFAMs containing neem tree extract were shown to be slightly toxic to Encarsia formosa and Orius laevigatus. The results of this study could be used to develop management models using natural enemies of control the insect pests; T. vaporariorum and thrips in semi-forcing culture of tomato.

• Journal of Ginseng Research
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• v.46 no.5
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• pp.666-674
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• 2022

Background: Ginsenosides and their metabolites have antidepressant-like effects, but the underlying mechanisms remain unclear. We previously identified 14-3-3 ζ as one of the target proteins of 20 (S)-protopanaxadiol (PPD), a fully deglycosylated ginsenoside metabolite. Methods: Corticosterone (CORT) was administered repeatedly to induce the depression model, and PPD was given concurrently. The tail suspension test (TST) and the forced swimming test (FST) were used for behavioral evaluation. All mice were sacrificed. Golgi-cox staining, GSK 3β activity assay, and Western blot analysis were performed. In vitro, the kinetic binding analysis with the Biolayer Interferometry (BLI) was used to determine the molecular interactions. Results: TST and FST both revealed that PPD reversed CORT-induced behavioral deficits. PPD also ameliorated the CORT-induced expression alterations of hippocampal Ser9 phosphorylated glycogen synthase kinase 3β (p-Ser9 GSK 3β), Ser133 phosphorylated cAMP response element-binding protein (p-Ser133 CREB), and brain-derived neurotrophic factor (BDNF). Moreover, PPD attenuated the CORT-induced increase in GSK 3β activity and decrease in dendritic spine density in the hippocampus. In vitro, 14-3-3 ζ protein specifically bound to p-Ser9 GSK 3β polypeptide. PPD promoted the binding and subsequently decreased GSK 3β activity. Conclusion: These findings demonstrated the antidepressant-like effects of PPD on the CORT-induced mouse depression model and indicated a possible target-based mechanism. The combination of PPD with the 14-3-3 ζ protein may promote the binding of 14-3-3 ζ to p-GSK 3β (Ser9) and enhance the inhibition of Ser9 phosphorylation on GSK 3β kinase activity, thereby activating the plasticity-related CREBeBDNF signaling pathway.

• Journal of Korean Society of Transportation
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• v.21 no.1
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• pp.127-136
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• 2003

Road safety is defined under the minimum design standard and design examination process is consisted of the standard according to current road design. However, road safety in practical way is correlative to not only all element of roads but also road shape, such as, between straight line and curved line and between curved lines. Also. it is related to alignments such as horizontal alignment and vertical alignment, and cross section. That is, the practical road design should be examined in both sides of 3 dimension and consecutiveness (consistency) as the actual road is a 3 - dimensional successive object. The paper presents a concept for acceleration to evaluate consistency of road considering actual road shape on 3-dimension. Acceleration of vehicle is influential to road consistency based on running state of vehicles and state of drivers. The magnitude of acceleration. especially, is a quite influential element to drivers. Based on above, the acceleration on each point on 3-D road can be calculated and then displacement can be done. Computation of acceleration means total calculation on each axis. Speed profile refers to “Development of a safety evaluation model for highway horizontal alignment based on running speed(Jeong, Jun-Hwa, 2001)” and then acceleration can be calculated by using the speed pronto. According to literature review, definition of acceleration on 3-D and g-g-g diagram are established. For example, as a result of the evaluation, if the acceleration is out of range, the road is out of consistency. The paper shows calculation for change of acceleration on imaginary road under minimum design standard and the change tried to be applied to consistency. However accurate acceleration is not shown because the speed forecasting model is limited and the paper did not consider state of vehicles (suspension, tires and model of vehicles). If speed pronto is defined exactly, acceleration is calculated on all road shapes, such as. compound curve and clothoid curve. and then it is appled to consistency evaluation. Unfortunately, speed forecasting model on 3 -D road and on compound curves have rarely presented. Speed forecasting model and speed profile model need to be established and standard of consistency evaluation need to developed and verified by experimental vehicles.

• Journal of the Korean Institute of Landscape Architecture
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• v.38 no.3
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• pp.75-82
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• 2010

This study formed an estimation of the visual characteristics of urban bridges in Yanghwaro in the Gyeongui Railroad Area using a landscape simulation. Existing theses have formerly only suggested directions for design based on visual preference, but there is as yet no research on the practical process of landscape design. As a result, it is difficult to directly apply this to bridge design. This study found a potential bridge site and presented a direction for bridge design in order to improve the image of the surrounding urban landscape by surveying the visual effects and landscape preferences of different bridge types. An urban landscape was produced using a landscape simulation model and was made the background for the survey. Five bridge types--Girder, Arch, Truss, Cable and Suspension--were selected and presented. The shapes of the bridges were selected based on the floor plan. The results of this study are as follows. In a preference analysis, every bridge except Girder was evaluated as a positive influence. When rating the image, 'artificial' was rated significantly higher than other traits when assessing the background image. When the Girder Bridge was introduced, 'stable' and 'orderly' were both rated highly while 'stable', 'beautiful', 'orderly' and 'interesting' were high with the introduction of the Arch Bridge. 'Beautiful', 'stable', and 'orderly' were given a high value in the introduction of the Truss Bridge and every image except 'natural', 'harmony' and 'orderly' were highly rated in the introduction of the Cable Bridge. Further, every image but 'natural' was highly rated with the introduction of the Suspension Bridge. Based on the analysis of the landscape, there is a difference in preference before and after modeling a bridge type, while the bridge itself is an influence when it is the main object of the simulated scene. This study researched only the shape of the bridge as a part of the landscape but other elements such as stability, economics, and construction are also factors in the design of a bridge. Stability, economics, construction and other factors must be considered when selecting a bridge type in the future.