• Transactions of the Korean Society of Mechanical Engineers A
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• v.36 no.11
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• pp.1447-1453
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• 2012

For an electromagnetic suspension (EMS)-type urban Maglev train using U-shaped electromagnets, both the vertical and the lateral air gaps for levitation are maintained only by the electromagnet. The train can run over curved rails without active lateral air gap control because the U-shaped electromagnet simultaneously produces both a levitation force and a guidance force, which is dependent on the levitation force. Owing to the passive control of the lateral air gap, the lateral vibration could exceed the limits of the lateral air gap and acceleration. In this study, dynamic analysis of a Maglev train is carried out, and the effectiveness of a lateral damper for vibration reduction is investigated. To more accurately predict the lateral vibration, a Maglev vehicle multibody model including air-sparing, guideway irregularities, electromagnets, and their controls is developed.

• Transactions of the Korean Society of Automotive Engineers
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• v.16 no.1
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• pp.144-151
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• 2008

The bi-modal vehicle is composed of two car-bodies and three axles. Each axle of the vehicle has an independent suspension and all wheels are steerable. Since the bi-modal vehicle has longer wheelbase than most urban buses, the All-Wheel-Steering(AWS) system is adapted for to ensure safe driving and proper turning radius on a curved road. This paper proposes an AWS control algorithm for stable driving of bi-modal vehicle. Steering angles and directions of each axle of bi-modal vehicle changed according to the driving environment and steering modes. In the case that front and rear axles should be steered in opposite directions is a negative mode, and the other case that the axles should be steered in the same direction is a positive mode. For example, in the positive mode, front and real axles are steered in the same direction, while in the negative mode, they are steered in the opposite direction. A multibody model of the vehicle is used to verify the performance of the steering algorithm and simulation results of 2WS are compared with those of AWS under the same condition.

• Wind and Structures
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• v.30 no.6
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• pp.547-558
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• 2020

With the development of economy and construction technology, more and more bridges are built in complex mountainous areas. Accurate assessment of wind parameters is important in bridge construction at complex terrain. In order to investigate the wind characteristics in the high-altitude difference area, a complex mountain terrain model with the scale of 1:2000 was built. By using the method of wind tunnel tests, the study of wind characteristics including mean wind characteristics and turbulence characteristics was carried out. The results show: The wind direction is affected significant by the topography, the dominant wind direction is usually parallel to the river. Due to the sheltering effect of the mountain near the bridge, the wind speed and wind attack angle along the bridge are both uneven which is different from that at flat terrain. In addition, different from flat terrain, the wind attack angle is mostly negative. The wind profiles obey exponential law and logarithmic law. And the fitting coefficient is consistent with the code which means that it is feasible to use the method of wind tunnel test to simulate complex terrain. As for turbulence characteristics, the turbulence intensity is also related to the topography. Increases sheltering effect of mountain increases the degree of breaking up the large-scale vortices, thereby increasing the turbulence intensity. Also, the value of turbulence intensity ratio is different from the recommended values in the code. The conclusions of this study can provide basis for further wind resistance design of the bridge.

• Journal of Pharmaceutical Investigation
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• v.31 no.3
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• pp.185-190
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• 2001

Human papillomavirus (HPV) infection is known to cause cervical cancers. Human papillomavirus-like particles (VLP) have been studied as preventive vaccines of cervical cancers. To develop VLP as a therapeutic gene carrier, we studied the method to encapsulate cytokine genes in virus-like particles. HPV type 16 capsid L1 genes were amplified by polymerase chain reaction and cloned into T vector. L1 gene was then inserted into baculovirus transfer vector. The clone of baculovirus encoding L1 gene was isolated and used to express L1 protein in Sf 21 insect cells. VLP were purified by CsCl density gradient and ultracentrifugation. VLP were disassembled to capsomer units by treatment of a reducing agent. Given that interleukin-2 (IL-2) genes have been used in anticancer gene therapy and as a molecular adjuvant, IL-2 cytokine plasmids were chosen as a model gene. IL-2 plasmids were incubated with the disassembled capsomer suspension. To reassemble the particles, the mixture of capsomers and cytokine plasmids was dialyzed. The disassembly and reassembly of VLP were confirmed by transmission electron microscopy. The entrapment of cytokine plasmids in reassembled VLP was tested by the stability of plasmids against DNase I. After treatment of reassembled virus-like particles with DNase I, discrete IL-2 DNA band was observed. Our results indicate that IL-2 cytokine plasmid (3.5 kb size) can be encapsulated in the virus-like particles, suggesting the potential of VLP as a gene delivery system. Moreover, VLP containing the adjuvant cytokine plasmids might function as more effective subunit vaccines.

• Journal of the Korean Chemical Society
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• v.54 no.3
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• pp.300-309
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• 2010

The ability of sodium-exchanged clay particles as an adsorbent for the removal of commercial dyes, Methylene blue (MB) and Malachite green oxalate (MG) from aqueous solutions has been investigated under various experimental conditions. The effect of the experimental parameters, such as pH solution, agitation time, adsorbate concentration and adsorbent dose were examined. Maximum adsorption of dyes, i.e. >90% has been achieved in aqueous solutions using 0.03 g of clay at a pH of 7 and 298 K for both dyes. The adsorption process was a fast and the equilibrium was obtained within the first 5 min. For the adsorption of both MB and MG dyes, the pseudo-second-order reaction kinetics provides the best correlation of the experimental data. The adsorption equilibrium results follow Langmuir and Dubini-Radushkevich (D-R) isotherms with high regression coefficients $R^2$ > 0.98. The mean free energies $E_a$ of adsorption from D-R model were 3.779 and 2.564 kj/mol for MB and MG respectively, which corresponds to a physisorption process.

• Journal of the Korean Society of Tobacco Science
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• v.28 no.2
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• pp.83-93
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• 2006

The aim of this study is to develop a simple and rapid field method for the determination of chloride content in tobacco green or cured leaves. To set up a practical process, several factors such as the proper sampling position of each leaf, the size of leaf lamina pieces for blending, the substitution of deionized water fur the extracting solution, 0.1N $HNO_3$, the submerging durations before blending, and the standing times before measuring were studied using flue-cured and burley tobacco with a stable and reproducible Chloride Meter, KRK CL-2Z. From the results, chloride contents of tobacco leaves could be analyzed with the Chloride Meter(CL-2Z) using the suspension of 14 mm diameter leaf disks after soaking in distilled water for 30 min., blending with a mini-blender for 30 seconds. The chloride contents measured in this way showed highly significant correlations with ones by the conventional potentiometric method using an ion meter-chloride electrode and $AgNO_3$ titration. In our method, the procedures such as drying, powdering and weighing samples, and using chemical reagents for extraction (0.1N $HNO_3$) and titration(0.028N $AgNO_3$) could be eliminated. These results suggest that the newly constructed method using the Chloride Meter is more efficient to determine the chloride content of tobacco leave in comparison with the one by the Ion $Meter:AgNO_3$ titration.

• 한국생물공학회:학술대회논문집
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• 2000.11a
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• pp.241-244
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• 2000

Continuous $H_2$ production from CO and water was studied in a trickle bed reactor(TBR) using Rhodopspedomonas palustris P4. To achieve high cell density, R. palustris P4 were cultivated by a fed-batch culture mode under chemoheterotrophic and aerobic condition, and final cell concentration was 13 g/L. TBR could provide sufficient residence time for CO to contact with cell suspension circulating TBR. The maximum CO uptake rate was found to be 16 mmol/L/hr at gas retention time of 50 min and CO partial pressure of 0.4 atm. In our correlation of the experimental data with mathematical model of TBR, the TBR operation with P4 was found to be lie in an intermediate state between mass transfer limitation and kinetic limitation. Due to the high cell density as well as hydrogen production activity in this study, TBR operation showed a superior performance to other previous reports on microbial hydrogen production.

• Asian Pacific Journal of Cancer Prevention
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• v.16 no.3
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• pp.915-921
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• 2015

Context: In the last half century, discovering, developing and introducing of clinical agents from marine sources have seen great successes, with examples including the anti-cancer compound trabectedin. However, with increasing need for new anticancer drugs, further exploration for novel compounds from marine organism sources is strongly justified. Objective: The major aim of this study was to evaluate the antitumor and antioxidant potential of Sargassum tenerrimum J.Agardh (Sargassaceae) on Ehrlich ascites carcinoma (EAC) in Swiss albino mice. Materials and Methods: An ethanol extract of S. tenerrimum (EEST) from whole algae was used to evaluate cytotoxicity followed by in vivo assessment of toxicity, using biochemical parameters including hepatic and non-hepatic enzymes. Antioxidant properties were examined in animals bearing EAC treated with daily oral administration of 100-300 mg/kg extract suspension. Results: Antitumor effects of EEST in EAC bearing mice was observed with LD50 1815 mg/kg. Parameters like body weight, tumor volume, packed cell volume, tumor cell count, mean survival time and increase in life span in animals in the EAC bearing animals treated with EEST 300 mg/kg was comparable with control group. Significant differences were also seen with changes in total protein content, hepatic enzymes contents, MDA level, and free radical scavenging enzymes in untreated vs. EEST treated group animals. Conclusions: Evaluation of antioxidant enzymes and hepatic enzymes in the EAC animal model treated with EEST exhibited similar effects as the positive control drug 5-flurouracil. S. tenerrimum extracts contain effective antioxidants with significant antitumor activity.

• Journal of Korea Water Resources Association
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• v.51 no.11
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• pp.959-969
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• 2018

The aging water pipes induce various problems, such as water supply suspension due to breakage, insufficient water pressure, deterioration of water quality, damage by sink holes, and economic losses due to water leaks. However, it is impractical and almost impossible to repair and/or replace all deteriorated water pipes simultaneously. Hence, it is required to quantitatively evaluate the deterioration rate of individual pipes indirect way to determine the rehabilitation order of priority. In this study, ANN(Artificial Neural Network)-Clustering method is suggested as a new approach to assess and assort the water pipes. The proposed method has been applied to a water supply network of YG-county in Jeollanam-do. To assess the applicability of the model, the evaluation results were compared with the results of the Numerical Weighting Method (NWM), which is being currently utilized in practice. The assessment results are depicted in a water pipe map to intuitively grasp the degree of deterioration of the entire pipelines. The application results revealed that the proposed ANN-Clustering models can successfully assess the water pipe deterioration along with the conventional approach of NWM.

• Journal of Korean Society of Coastal and Ocean Engineers
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• v.7 no.3
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• pp.277-288
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• 1995

Prediction of turbidity due to fine-grained bed material load under wave action is critical to any assessment of anthropogenic impart on the coastal or lacustrine environment Waves tend to loosen mud deposits and generate steep suspension concentration gradients, such that the sediment load near the bottom is typically orders of magnitude higher than that near the surface. In a physically realistic but simplified manner, a simple mass conservation principle has been used to simulate the evolution of fine sediment concentration profiles and corresponding erodible bed depths under progressive, nonbreaking wave action over mud deposits. Prior field observations support the simulated trends. which reveal the genesis of a near-bed. high concentration fluidized mud layer coupled with very low surficial sediment concentrations. It is concluded that estimation of the depth of bottom erosion requires an understanding of mud dynamics and competent in situ sediment concentration profiling. Measurement of sediment concentration at the surface alone, without regard to the near-bed zone, can lead to gross underestimation of the erodible bed depth.