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A study on the correlations between salivary levels of lysozyme, lactoferrin and secretory Immunoglobulin A to Streptococcus mutans and caries susceptibility (치아우식감수성과 타액내 Lysozyme, Lactoferrin 및 Streptococcus mutans에 대한 secretory IgA 수준과의 상관관계에 관한 연구)

  • Yoo, Hyeon-Mee;Kwon, Hyuk-Choon
    • Restorative Dentistry and Endodontics
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    • v.19 no.2
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    • pp.372-383
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    • 1994
  • Saliva plays an important role in modulating the oral microbial ecology. And it is suggested to influence the initiation and progression of the dental caries. To evaluate the correlations between the salivary antimicrobial agents and the caries susceptibility, the 51 subjects were divided into 3 groups according to caries experience ; caries resistant group, medium caries susceptible group, and high caries susceptible group. Stimulated whole saliva was collected, and the salivary levels were measured for lysozyme, lactoferrin, and secretory-IgA to Streptococcus mutans. The lysozyme level was estimated using Micrococcus diffusion plate, lactoferrin level was determined with a non-competitive avidin-biotin enzyme immunoassay, and the titer of secretory IgA to Streptococcus mutans was assayed with ELISA. The results were as follows: 1. Lysozyme levels of each group showed no significant difference statistically (p>0.05). 2. The caries resistant group and the medium caries susceptible group had significantly higher levels of lactoferrin than the high caries susceptible group (p<0.05). But no clear difference was observed between the caries resistant group and the medium caries susceptible group(p>0.05). 3. The caries resistant group and the medium caries susceptible group showed relatively higher levels of the secretory IgA to Streptococcus mutans than the pigh caries susceptible group, but no significant difference was observed statistically (p>0.05).

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Characteristics of Resistant Lines to High-Temperature Injury in Ginseng (Panax ginseng C. A. Meyer)

  • Lee, Joon-Soo;Lee, Jang-Ho;Ahn, In-Ok
    • Journal of Ginseng Research
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    • v.34 no.4
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    • pp.274-281
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    • 2010
  • This experiment was conducted to examine ginseng lines resistant and susceptible to high-temperature injury and to investigate characteristics of the selected lines: leaf burning phenomenon, chlorophyll content, quantum yield, and maximum light interception rate. The leaf burning phenomenon incidence rates of the resistant lines Yunpoong, high-temperature injury resistance (HTIR)1, HTIR2, and HTIR3 were low: 5.8%, 3.6%, 4.0%, and 1.9%, respectively. Resistance of the susceptible lines Chunpoong, high-temperature injury susceptible (HTIS)1, and HTIS2 was high: 58.5%, 23.2%, and 21.7%, respectively. The chlorophyll content (SPAD value) of the resistant lines Yunpoong, HTIR1, HTIR2, and HTIR3, which were exposed to high temperatures and intense light, remained as high at 24.8, 27.9, 24.9, and 30.6, respectively, but that of the susceptible lines Chunpoong, HTIS1, and HTIS2 was low at 21.0, 21.1, and 20.1, respectively. During the summer season, the quantum yield of the resistant lines (Yunpoong, HTIR1, HTIR2, and HTIR3) changed little, but that of the susceptible lines (Chunpoong, HTIS1, and HTIS2) changed dramatically. The maximum light interception rate (Fm/Fv value) for the resistant lines (Yunpoong, HTIR1, HTIR2, and HTIR3) was as high as 0.848, 0.794, 0.805, and 0.813, respectively, while that of the susceptible lines (Chunpoong, HTIS1, and HTIS2) was 0.678, 0.642, and 0.717, respectively. Based on these results, the high-temperature injury-resistant lines seemed to be less susceptible to high light, even at high temperatures. Future studies on red ginseng quality and its active ingredients in resistant ginseng lines and field experimentation will be conducted to verify the potential of the resistant lines.

Differential Tolerance of Plant Species to Protoporphyrinogen Oxidase-Inhibiting Oxyfluorfen (Protoporphyrinogen oxidase 저해형 Oxyfluorfen에 대한 식물종간 내성차이)

  • Kuk, Yong-In;Guh, Ja-Ock
    • Korean Journal of Environmental Agriculture
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    • v.15 no.3
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    • pp.316-324
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    • 1996
  • The four tolerant and one susceptible plant to oxyfluorfen were selected from 26 species and investigated for the inhibition of protox activity, the PPIX accumulation, and the activity of antioxidative enzymes by oxyfluorfen treatment. When treated, the oxyfluorfen-tolerant plant species showed a less decrease in fresh weight and height than the susceptible one. The susceptible barnyardgrass showed more inhibition of protox activity due to the treatment of oxyfluorfen than the tolerant species. Especially at the concentration of $10^{-6}M$, protox activity of the susceptible barnyardgrass was inhibited completely, but tolerant species maintained $25{\sim}45%$ of the activity. Under the light and dark condition, the susceptible barnyardgrass showed more PPIX accumulation than the tolerant.

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Estimation of Heterosis and Combining Ability in Hybrids between Resistant and Susceptible Bivoltine Breeds of Silkworm Bombyx mori to Densonucleosis Virus1 (BmDNV1)

  • Rao, P. Sudhakara;Nataraju B.;Balavenkatasubbaiah M.;Dandin S.B.
    • International Journal of Industrial Entomology and Biomaterials
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    • v.13 no.2
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    • pp.73-77
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    • 2006
  • Combining ability and hybrid vigour analysis was carried out in hybrids between newly developed non-susceptible lines to BmDNV1 and popular bivoltine breeds for certain quantitative traits viz. Pupation rate, Cocoon yield, Cocoon weight, Cocoon shell weight and Cocoon shell ratio, Survival rate against BmIFV and BmNPV. General combining ability (GCA) effects revealed that among the lines CSR2DR was found good general combiner exhibiting significant GCA effects for six characters, out of seven traits evaluated. Among testers CSR28DR was found as good combiner exhibiting significant GCA effects for six traits. Out of 36 hybrids made between $resistant{\times}resistant,\;resistant{\times}susceptible\;and\;susceptible{\times}susceptible$ breeds, one hybrid $CSR21DR{\times}CSR28DR$ exhibited significant SCA effects for six traits. The selected hybrid $CSR21DR{\times}CSR28DR$ also exhibited significant positive heterosis and heterobeltiosis expressions for maximum traits and could be exploited as commercial silkworm hybrid resistant to important viral diseases.

A Study on the Prediction of Parturient Syndrome in Holstein Cows (젖소에서의 산욕기질병 발생예견에 관한 연구)

  • Youn Hwa-Young;Choi Hee-In
    • Journal of Veterinary Clinics
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    • v.2 no.1
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    • pp.133-141
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    • 1985
  • In order to establish a method predicting susceptible cows to the parturient syndrome, various serum chemical parameters (calcium, phosphorus, Ca/P, magnesium, cholesterol, total protein, albumin, globulin, A/G, total lipid, non-esterified fatty acid(NEFA) and aspartate aminotransferase(AST)) were measured during late pregnancy and their relationships with periparturient diseases were investigated during puerpural period. The results obtained were as follows : 1. The factors affecting the prediction of susceptible cows to parturient syndrome were calcium, magnesium, total protein, globulin, A/G ratio and total lipid at 30 day antepartum and the diagnosability was 70.7%. 2. In the experimental cows producing more than 21kg of milk per day, the factors affecting the prediction of susceptible cows to parturient syndrome were calcium, NEFA and A/G ratio at 30 day antepartum and the diagnosability was 66.7%. 3. In the experimental cows calved more than 3 times, the factors affecting the perdiction of susceptible cows to parturient syndrome were calcium, total protein, albumin and NEFA at 30 day antepartum and the diagnosability was 83.3%.

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In Vitro Inhibitory Activities of Essential Oils from Rosmarinus officinalis L. Against Antibiotic-Susceptible and Resistant Strains of Some Pathogenic Bacteria (Rosmarinus officinalis 정유의 수종 항생제 감수성 및 내성 균주에 대한 억제효과)

  • Shin, Seung-Won
    • Korean Journal of Pharmacognosy
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    • v.36 no.3 s.142
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    • pp.252-256
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    • 2005
  • The in visto inhibitory activities of essential oils of the Rosmarinus officinalis as well as its main constituents were evaluated against antibiotic-susceptible and -resistant strains of Staphylococcus aureus, streptococcus pneumoniae, Salmonella enteritidis and S. typhimurium. The essential oil fraction of R. officinalis and its main components, 1,8-cineole and camphor, exhibited significant inhibitory activities against most of the tested strains in this study, with MICs(minimum inhibitory concentrations) racing from 0.5mg/ml to 16mg/ml. The total oil fraction showed higher activity than its main components, 1,8-cineole and camphor against S. aureus strains. No remarkable differences were evident between MICs of the susceptible and resistant strains of S. aureus. Among the tested strains, S. pneumoniae CCARM 3523, the resistant strain to norfloxacin, oxacillin and erythromycin exhibited significantly lower sensitivity to the tested oils than antibiotic-susceptible strain. The oils revealed mostly higher inhibitory activity against S. typhimurium than against S. enteritidis.

Relationship between some chemical components in the rice plants and varietal reaction to blast disease (도열병 품종저항성과 도체내 성분과의 관계)

  • Baek Soo Bong
    • Korean journal of applied entomology
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    • v.9 no.1
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    • pp.15-20
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    • 1970
  • An investigation was carried out to know the relationship between resistance of rice varieties to blast disease and the chemical components, especially total nitrogen and total sugars, in the rice plants. The results sic summarized as follow: 1) The nitrogen contents in the resistant variety were less than those of susceptible one, and sugar contents were reversed. Accordingly, the C/N ratio in the resistant variety was higher than that obtained by susceptible one. 2) The free amino acids contents, especially, Glutamine, Valine, Leusine and Iso-leusine, in the resistant varieties were more than those of the susceptible varieties. 3) The starch synthetic activity of rice leaves in the resistant varieties was higher than that of susceptible one in fructose and glucose solutions, but it was reversed in sucrose solution. 4) When more nitrogen was dressed, the total nitrogen content of rice leaves was increased than the ordinary dressing. The rate of increase in nitrogen content in resistant variety was lower than those of the susceptible. The total sugar content of rice plants dressed more nitrogen was decreased at early tillering stage, but increased at maximum tillering stage. It seemed that the rate of increase of total sugar in the resistant variety was higher than those of the susceptible.

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Comparison of DNase activities from excretory/secretory productsof Haemonchus contortus fenbendazole-resistantand -susceptible isolates (Fenbendazole에 저항성과 감수성을 지닌 염전위충의 분비배설물에서의 DNase 활성 비료)

  • Kwak, Dongmi
    • Korean Journal of Veterinary Research
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    • v.44 no.3
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    • pp.455-462
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    • 2004
  • Change in ${\beta}$-tubulin nucleic acid and protein sequences was the only known difference between Haemonchus contortus fenbendazole (FBZ)-resistant and -susceptible isolates. This change was sufficient to determine the pathologic effect induced by FBZ treatment. This research was initiated to investigate further differences from these two isolates. Since ${\beta}$-tubulin is involved in formation of microtubule, which has functions in secretory vesicle transport, DNase activities from excretory/secretory products (ESP) of the two isolates were compared, based on pH, sensitivity to DNase inhibitors, molecular masses and production of 3'-OH. The most significant difference detected was that a 38.5 kDa DNase activity was identified from ESP of H. contortus FBZ-susceptible isolates but not from those of H. contortus FBZ-resistant isolates. However, it was shown that the 38.5 kDa DNase is expressed with similar level of activity in intestine and whole worm of H. contortus FBZ-resistant and -susceptible isolates. This result demonstrated that the secretory transport pathway of the 38.5 kDa DNase was inhibited by unknown mechanisms, which may be related with ${\beta}$-tubulin sequence change in FBZ-resistant isolates. Other DNases of 34, 36 and 37 kDa were detected from ESP of both H. contortus FBZ-resistant and -susceptible isolates. Overall DNase activities found from ESP of these two isolates were not inhibited by 10 mM EDTA at pH 5.0, but largely inhibited by pH 7.0. In addition, DNase activities in two isolates produced DNA fragments with mixtures of 3'- hydroxyls (OH) and 3'-phosphates (P) at each pH although the 3'-end labeling ratios at pH 5.0 and 7.0 were shown different. Identification of inhibition of the 38.5 kDa DNase secretion in FBZ-resistant isolates suggests existence of further differences, in addition to ${\beta}$-tubulin sequence change, in two isolates. This shows complex effect of FBZ on H. contortus biological mechanisms.

Assessing the Landslide Susceptibility of Cultural Heritages of Buyeo-gun, Chungcheongnam-do (충남 부여군 문화재의 산사태 민감성 평가)

  • Kim, Jun-Woo;Kim, Ho Gul
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.25 no.5
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    • pp.1-13
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    • 2022
  • The damages caused by landslides are increasing worldwide due to climate change. In Korea, damages from landslides occur frequently, making it necessary to develop the effective response strategies. In particular, there is a lack of countermeasures against landslides in cultural heritage areas. The purpose of this study was to spatially analyze the relationship between Buyeo-gun's cultural heritage and landslide susceptible areas in Buyeo-gun, Chungcheongnam-do, which has a long history. Nine spatial distribution models were used to evaluate the landslide susceptibility, and the ensemble method was applied to reduce the uncertainty of individual model. There were 17 cultural heritages belonging to the landslide susceptible area. As a result of calculating the area ratio of the landslide susceptible area for cultural heritages, the cultural heritages with 100% of the area included in the landslide susceptible area were "Standing statue of Maae in Hongsan Sangcheon-ri" and "Statue of King Seonjo." More than 35% of "Jeungsanseong", "Garimseong", and "Standing stone statue of Maitreya Bodhisattva in Daejosa Temple" belonged to landslide susceptible areas. In order to effectively prevent landslide damage, the application of landslide prevention measures should be prioritized according to the proportion belonging to the landslide susceptible area. Since it is very difficult to restore cultural properties once destroyed, preventive measures are required before landslide damage occurs. The approach and results of this study provide basic data and guidelines for disaster response plans to prevent landslides in Buyeo-gun.

Different Physiological Activity of Selected Rice Cultivars to Diphenylether Herbicide, Oxyfluorfen - IV. Different Activity of Antioxidative Enzymes (Oxyfluorfen에 대한 내성(耐性) 감수성(感受性) 벼품종(品種)의 생리활성(生理活性) 기구(機構) - IV. 항산화효소(抗酸化酵素) 활성(活性))

  • Kuk, Y.I.;Guh, J.O.
    • Korean Journal of Weed Science
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    • v.16 no.3
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    • pp.210-220
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    • 1996
  • Ten-day-old seedlings of the oxyfluorfen-tolerant and -susceptible rice cultivars with barnyardgrass, a typical susceptible weed were soaked in oxyfluorfen at $10^{-6}M$ for 2 hrs kept for 24 hrs in the dark, and exposed to light for 0, 2, 4, or 6 hrs to investigate changes in the activity and isozyme of antioxidative enzymes. The activities of antioxidative enzymes of APOX, CAL, POX, NR, GR, MDAR and SOD in the tolerant and susceptible rice cultivate themselves didn't show any difference but the activity in the susceptible barnyardgrass was very low in comparison with rice cultivars. The activity of lipoxygenase tended to be some slightly higher in the susceptible rice cultivars and barnyardgrass than in the tolerant rice cultivars. The activities of MDAR, POX, GR and SOD, antioxidative enzymes, were higher in the tolerant rice cultivars than in the susceptible rice cultivars and barnyardgrass after the treatment of oxyfluorfen. After the treatment of oxyfluorfen, in the change of POX isozyme, the activity of C band in the tolerant rice cultivars increased with increased concentration but it didn't in the susceptible rice cultivar. The activity of B band decreased slightly at $10^{-4}M$ in the susceptible barnyardgrass. Isozyme of GR, SOD and AO by the treatment of oxyfluorfen, the activity of each band between the tolerant and susceptible rice cultivars showed no difference but GR isozyme C band was disappeared in the susceptible barnyardgrass at $10^{-4}M$. In the change of esterase isozyme resulting from the treatment of oxyfluorfen, the activities of B, C and D bands decreased more in the susceptible rice cultivars than in tolerant rice cultivars, and A band was disappeared in the susceptible barnyardgrass at $10^{-4}M$.

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