• Structural Engineering and Mechanics
• /
• 제4권6호
• /
• pp.601-612
• /
• 1996

To simulate the large-scale failure evolution with current computational facilities, a simple approach, that catches the essential feature of failure mechanisms, must be available so that the routine use of failure analysis is feasible. Based on the previous research results, a simple analysis procedure is described in this paper for failure simulation. In this procedure, the evolution of localization is represented by a moving surface of discontinuity, and the transition between continuous and discontinuous failure modes are described via the moving jump forms of conservation laws. As a result, local plasticity and damage models, that are formulated based on thermodynamic restrictions, are still valid without invoking higher order terms, and simple integration schemes can be designed for the rate forms of constitutive models. To resolve localized large deformations and subsequent cracking, an efficient structural solution scheme is given for Static and dynamic problems.

• 센서학회지
• /
• 제12권6호
• /
• pp.249-257
• /
• 2003

환자의 체내에 삽입하여 여러 가지 생체정보를 무선으로 전송하는 형태의 텔레메트리 기술은 환자들의 불편함을 해소하고 기존의 진단 한계를 극복하는데 큰 기여를 할 것으로 기대되고 있다. 체내에서 생체신호를 외부의 전송하는 시스템의 경우, 정확한 질병지점을 판단하기 위해서는 체내의 텔레메트리 모듈의 위치를 체외에서 정착하게 파악할 수 있는 기술이 필수적이다. 본 논문에서는 8개의 안테나를 고정된 위치에 두고, 비선형 연립방정식의 해석을 통하여 체내에 삽입된 송신모듈의 위치를 결정하는 방법을 제안하고 해석하였다.

• 한국생물공학회:학술대회논문집
• /
• 한국생물공학회 2003년도 생물공학의 동향(XIII)
• /
• pp.468-472
• /
• 2003

Recently, genetic engineering techniques have been used to display various heterologous peptides and proteins (enzyme, antibody, antigen, receptor and fluorescence protein, etc.) on the yeast cell surface. Living cells displaying various enzymes on their surface could be used repeatedly as 'whole cell biocatalysts' like immobilized enzymes. We constructed a yeast based whole cell biocatalyst displaying T. reesei cellobiohydrolase I (CBH I ) on the cell surface and endowed the yeast-cells with the ability to degrade cellulose. By using a cell surface engineering system based on ${\alpha}-agglutinin,$ CBH I was displayed on the cell surface as a fusion protein containing the N-terminal leader peptide encoding a Gly-Ser linker and the $Xpress^{TM}$ epitope. Localization of the fusion protein on the cell surface was confirmed by confocal microscopy. In this study, we report on the genetic immobilization of T. reesei CBH I on the S. cerevisiae and hydrolytic activity of cell surface displayed CBH I.

• Journal of Microbiology and Biotechnology
• /
• 제12권4호
• /
• pp.628-634
• /
• 2002

A microfilament-based motility in Toxoplasma gondii (T. gondii) Is involved in host cell invasion, yet the exact mechanism has not yet been determined. Accordingly, the current study examined the localization of actin and tubulin in T gondii using immunofluorescent (IF) and immunogold staining for electron microscopy. Indirect immunofluorescence (IF) staining using anti-actin and anti-tubulin monoclonal antibodies (mAbs) revealed localization of fluorescence on the entire surface of the tachyzoites. The actin in T. gondii was observed by immunogold staining, and the gold particles were seen on the surface, especially at the anterior end and in the cytoplasm of the parasite. However, there were no gold particles in the nucleus, rhoptries, and dense granules. The tubulin in T gondii was located on the surface and in the cytoplasm of the tachyzoites in the extracellular parasite, compared with anterior part of tachyzoites in the intracellular parasite. The antigens of T gondii recognized by anti-actin mAb were 107 kDa, 50 kDa, 48 kDa, and 40 kDa proteins, while those recognized by anti-tubulin mAb were 56 kDa, 52 kDa, and 34 kDa proteins. Tachyzoites of T gondii pretreated with the actin inhibitor, cytochalasin D (20 $\mu\textrm{g}$/ml), and tubulin inhibitor, colchicine (2$\times$10$\^$-6/ M), for 30 min at 37$\^{C}$ were used to infect the isolated mouse macrophages (tachyzo ites:macrophage=2:1). Pretreatment with the inhibitors resulted in lower multiplication of tachyzoites within the macrophages than in the untreated group 18 h post infection (p<0.05). Therefore, the present results suggest that actin and tubulin appear to be involved in the invasion of and multiplication in host cells.

• 한국해양학회지
• /
• 제23권1호
• /
• pp.13-23
• /
• 1988

울기부근의 표층냉수 분포를 설명할수 있는 간단한 모델이 제시되었다. 본 모델에서는 좁은 연안역에서 냉수가 공급되고 이것이 부근 해류에 의하여 운반되며 외양으로 확산됨을 보여주고 있다. 또한 수직적인 열교환(대기 및 저층수와의 상호작용을 통한)에 의한 냉수의 소멸도 중요하게 작용하고 있다. 이러한 물리적 요인들을 감안한 간단한 수치 모델의 결과는 실측치와 잘 일치하고 있다. 냉수의 중심부는 해류가 연안으로 부터 분리되는 점에서 나타남이 밝혀졌다.

• 대한기계학회:학술대회논문집
• /
• 대한기계학회 2002년도 학술대회지
• /
• pp.779-782
• /
• 2002

We examine the problem in which porous/viscoelastic compliant thin plates are subject to pressure fluctuations under transitional or turbulent boundary layer. Measurements are presented of the frequency spectra of the near-field pressure and radiated sound by compliant surface. A porous plate consisting of 5mm thick, open-cell foam with fabric covering and a viscoelastic painted plate of 1mm thick over an acoustic board of 4m thick were placed over a rigid surface in an anechoic wind tunnel. Streamwise velocity and wall pressure measurements were shown to highly attenuate the convective wall pressure energy when the convective wavenumber ($k_{ch}$) was 3.0 or more. The sound source localization on the compliant walls is applied to the measurement of radiated sound by using an acoustic mirror system.

• 대한기계학회논문집B
• /
• 제27권3호
• /
• pp.294-301
• /
• 2003

We examine a problem in which porous/viscoelastic compliant thin plates are subject to pressure fluctuations under transitional or turbulent boundary layer. Measurements are presented of the frequency spectra of the near-field pressure and radiated sound by compliant surface. A porous plate consisting of 5mm thick. open-cell foam with fabric covering and a viscoelastic-painted plate of 1mm thick over an acoustic board of 4mm thick were placed over a rigid surface in an anechoic wind tunnel. Streamwise velocity and wall pressure measurements were shown to highly attenuate the convective wall pressure energy when the convective wavenumber (k$_{c}$h) was 3.0 or more. The sound source localization on the compliant walls is applied to the measurement of radiated sound by using an acoustic mirror system.

• 제어로봇시스템학회논문지
• /
• 제15권2호
• /
• pp.218-224
• /
• 2009

This paper proposes an effective SLAM based on redundant extended Kalman filter for robot navigation in an irregular surface to enhance the accuracy of robot's pose. To establish an accurate model of a caterpillar type robot is very difficult due to the mechanical complexity of the system which results in highly nonlinear behavior. In addition, for robot navigation on an irregular surface, its control suffers from the uncertain pose of the robot heading closely related to the condition of the floor. We show how this problem can be overcome by the proposed approach based on redundant extended Kalman filter through some computer simulation results.

• 한국전기화학회:학술대회논문집
• /
• 한국전기화학회 2004년도 수소연료전지공동심포지움 2004논문집
• /
• pp.287-292
• /
• 2004

In order to understand the relationship between molecular structure of Metal-Organic Framework(MOF) and capacity of hydrogen absorption, quantum mechanical calculations and grand canonical Monte Carlo simulations have been carried out on a series of MOF-5 having various organic linkers. The calculation results about specific surface area and electron density for various frameworks indicated that the capacity of the hydrogen storage is largely dependent on effective surface area rather than the free volume. Based on the iso-electrostatic potential surface from density functional calculation and the amount of adsorbed hydrogens from grand canonical Monte Carlo calculation, it was also found that the electron localization ground organic linker plays an important role in hydrogen capacity of MOFs.

• BMB Reports
• /
• 제49권10호
• /
• pp.542-547
• /
• 2016

Acid-sensing ion channels (ASICs) are proton-gated cation channels widely expressed in the nervous system. Proton sensing by ASICs has been known to mediate pain, mechanosensation, taste transduction, learning and memory, and fear. In this study, we investigated the differential subcellular localization of ASIC2a and ASIC3 in heterologous expression systems. While ASIC2a targeted the cell surface itself, ASIC3 was mostly accumulated in the ER with partial expression in the plasma membrane. However, when ASIC3 was co-expressed with ASIC2a, its surface expression was markedly increased. By using bimolecular fluorescence complementation (BiFC) assay, we confirmed the heteromeric association between ASIC2a and ASIC3 subunits. In addition, we observed that the ASIC2a-dependent surface trafficking of ASIC3 remarkably enhanced the sustained component of the currents. Our study demonstrates that ASIC2a can increase the membrane conductance sensitivity to protons by facilitating the surface expression of ASIC3 through herteromeric assembly.