• 한국지능시스템학회논문지
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• 제24권3호
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• pp.259-264
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• 2014

본 연구는 자기 홀 센서의 특성으로 인해 실내 환경에서만 이용이 되었던 자기/자기-자이로유도 타입의 무인운반차를 실외 환경에서도 적용이 가능하도록 실외 주행용 자기 위치측정 장치를 설계 및 제작하는 것이다. 현재 이용되고 있는 자기 위치측정 장치는 측정 높이가 30mm로 바닥 환경이 고르고 평평한 실내 환경에 적합한 구조이다. 하지만 바닥 환경이 울퉁불퉁하거나 불균형적인 실외환경에 이용되는 무인운반차에는 부적합하다. 그 이유는 무인운반차 서스펜션이 부착되게 되고, 이 때 자기 위치측정 장치의 부착높이가 30mm 이하로 무인운반차에 가해진 충격으로 인해 장치와 바닥과의 충돌이 발생하게 되면 장치가 파손되기 때문이다. 따라서 실외 자기유도 무인운반차에 적용하기 위해서는 100mm 이상의 측정 높이를 가지는 자기 위치측정 장치가 필요하다. 현재 자기위치측정 장치의 성능 향상 및 개발에 관련된 다양한 연구들이 진행되었지만, 다양한 자기 홀 센서를 분석하여 본 논문에서는 자기위치측정 장치를 설계 및 제작하였고, 자기 홀 센서의 특성 정보를 이용한 특성 함수를 이용해 자성체의 위치를 검출하였다. 실험을 위하여 알루미늄을 이용한 실험 장비를 제작하였으며, 제안된 자기 위치측정 장치를 이용하여 실험한 결과 위치측정 정밀도 오차는 10mm 이하이고, 측정 높이는 평균 150mm 로 실외 자기유도 무인운반차에 적합한 것을 확인하였다.

• Maxillofacial Plastic and Reconstructive Surgery
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• 제18권3호
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• pp.411-427
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• 1996

The purpose of this study was to observe the healing process and the distribution of fibronectin in injured condylar cartilage and bone by using LM and SEM. In order to perform this study, 40 male rat, weighing about 250g were selected. Under general anesthesia with Pentobarbital sodium, condylar cartilage and neck bone were resected. Then, the wound was irrigated with saline and closed with 5-0 chromic catgut and 4-0 silk by layer-to-layer suturing. The experimental rats were sacrificed by perfusion with 3% paraformaldehyde at 1st and 4th week after operation. The condylar process and surrounding tissues were cut, demineralized, dehydrated and embedded in paraffin. The histological observation of the specimens in LM level was performed after H-E stain and Azan stain. For localization of fibronectin, immunostaining was achieved by the avidin-biotin complex method. To study the change on condylar surface, the specimens were dehydrated, dried, gold coated and were observed with a scanning electron microscope(Hitachi S-2300). The results were as follows ; 1. The cartilage group and the bone group were repaired with epiphyseal cartilage layer on the cut surface as the normal control group. 2. The cut surface was repaired more quickly in the cartilage group than in the bone group. 3. Chondrocytes, diferentiated during healing, were stained strongly to anti-fibronectin, and fibronectin was supposed to participatein chondrocyte differentiation and cartilagenous matrix formation. 4. Fibronectin was distributed more in the new bone than in the old bone, and the osteoblasts surrounding it were also stained strongly. Fibronectin was supposed to participate in new bone matrix formation. 5. Fibronectin is supposed to be associated with the differentiation, migration and adhesion of chondrocyte and osteoblast and to participate in endochondral bone formation.

• Smart Structures and Systems
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• 제14권5호
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• pp.811-830
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• 2014

A numerical study has been carried out to simulate an innovative monitoring procedure to detect and localize damage in reinforced concrete beams retrofitted with carbon fiber reinforced polymer (CFRP) unidirectional laminates. The main novelty of the present simulation is its ability to conduct the electromechanical admittance monitoring technique by considerably compressing the amount of data required for damage detection and localization. A FEM simulation of electromechanical admittance-based sensing technique was employed by applying lead zirconate titanate (PZT) transducers to acquire impedance spectrum signatures. Response surface methodology (RSM) is finally adopted as a tool for solving inverse problems to estimate the location and size of damaged areas from the relationship between damage and electromechanical admittance changes computed at PZT transducer surfaces. This statistical metamodel technique allows polynomial models to be produced without requiring complicated modeling or numerous data sets after the generation of damage, leading to considerably lower cost of creating diagnostic database. Finally, a numerical example is carried out regarding a steel-reinforced concrete (RC) beam model monotonically loaded up to its failure which is also retrofitted by a CFRP laminate to verify the validity of the present metamodeling monitoring technique. The load-carrying capacity of concrete is predicted in the present paper by utilizing an Ottosen-type failure surface in order to better take into account the passive confinement behavior of retrofitted concrete material under the application of FRP laminate.

• 한국자기학회지
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• 제14권3호
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• pp.94-98
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• 2004

MgCCo$_3$에서 Mg와 Co로 이루어진 (001)표면(MgCo-Term) 또는 C와 Co로 이루어진 (001표면(CCo-term)의 전자구조와 자성을 FLAPW 에너지띠 방법을 이용하여 이론적으로 계산하였다. MgCo-Term의 경우 표면에서 Co원자의 자기모멘트는 1.00$\mu$$_{B}$로서 가운데층에 비하여 많이 증가하였고 표면 바로 밑층에서는 가운데층과 비슷한 값을 보였다. CCo-Term의 경우 Co원자의 자기모멘트는 표면에서 뿐만 아니라 표면 바로 밑층에서도 증가하여 각각 0.75와 0.80$\mu$$_{B}$의 값을 나타내고 있다. C와 Mg원자는 두 경우 모두 Co원자와 반대방향으로 스핀분극되었다. 계산된 상태밀도로부터 이러한 표면에서의 자기모멘트의 증가는 Co-3d 띠의 국소화에 의함을 알 수 있었고 여기서 Co-3d와 C-2p 사이의 띠 혼성이 중요한 역할을 하였다.

• Investigative Magnetic Resonance Imaging
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• 제14권2호
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• pp.126-133
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• 2010

목적 : 뇌피질이형성증에서 3차원 뇌표면연출영상의 유용성을 평가하고자 하였다. 대상 및 방법 : 19명의 국소적 뇌피질이형성증을 대상으로 MP-RAGE영상을 이용하여 3차원 뇌 표면연출영상을 얻었다. 비정상적인 뇌이랑과 뇌고랑의 해부학적 위치, 침범부위의 뇌이랑과 뇌고랑의 형태와 모양을 평가하였다. 결과 : 비정상적인 뇌이랑과 뇌고랑의 모양은 19명 중 18명의 환자에서 관찰되었다. 병변 부위의 뇌이랑과 뇌고랑의 형태와 배열, 침범모양은 뇌표면연출영상에서 명확하게 평가되었다. 무뇌회증에서는 엽이 구분되지 않았고, 뇌고랑이 없고 두껍고 매끄러운 뇌이랑이 하나의 엽으로 되어 있었다. 분열뇌증에서는 분열틈을 중심으로 여러 뇌이랑이 바퀴모양을 형성하고, 넓은 뇌이랑을 가지고 있었다. 일측성 거대뇌증에서는 병변측 대뇌반구가 커져 있었고, 두껍고 넓은 뇌이랑을 가지고 있었다. 선천성 양측 실비안주위 증후군에서는 섬피질이 노출되어 있었고, 병변 부위의 뇌이랑은 두꺼워져 있었다. 국소적 뇌피질이형성증에서는 균일하지 않은 톱니 모양이나 두껍고 커진 뇌이랑이 보였다. 이중 피질 증후군에서는 뇌이랑과 뇌고랑의 이상소견은 보이지 않았다. 결론 : 뇌피질이형성증 환자에서 3차원 뇌표면연출영상은 비정상적인 뇌피질의 뇌이랑과 뇌고랑 의 모양을 평가할 수 있고 정확한 병변의 위치를 평가하는 데 유용하다. 뇌표면연출영상은 수술 전 계획을 세우는 데 유용한 정보를 제공한다.

• Parasites, Hosts and Diseases
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• 제54권4호
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• pp.385-391
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• 2016

The discovery and understanding of antigenic proteins are essential for development of a vaccine against malaria. In Plasmodium falciparum, Pf92 have been characterized as a merozoite surface protein, and this protein is expressed at the late schizont stage, but no study of Pv92, the orthologue of Pf92 in P. vivax, has been reported. Thus, the protein structure of Pv92 was analyzed, and the gene sequence was aligned with that of other Plasmodium spp. using bioinformatics tools. The recombinant Pv92 protein was expressed and purified using bacterial expression system and used for immunization of mice to gain the polyclonal antibody and for evaluation of antigenicity by protein array. Also, the antibody against Pv92 was used for subcellular analysis by immunofluorescence assay. The Pv92 protein has a signal peptide and a sexual stage s48/45 domain, and the cysteine residues at the N-terminal of Pv92 were completely conserved. The N-terminal of Pv92 was successfully expressed as soluble form using a bacterial expression system. The antibody raised against Pv92 recognized the parasites and completely merged with PvMSP1-19, indicating that Pv92 was localized on the merozoite surface. Evaluation of the human humoral immune response to Pv92 indicated moderate antigenicity, with 65% sensitivity and 95% specificity by protein array. Taken together, the merozoite surface localization and antigenicity of Pv92 implicate that it might be involved in attachment and invasion of a merozoite to a new host cell or immune evasion during invasion process.

• Bulletin of the Korean Chemical Society
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• 제33권2호
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• pp.651-656
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• 2012

Cationic immunoliposomes were prepared by conjugation of Fab' fragments of the recombinant humanized monoclonal antibody (HuCC49) against tumor-associated glycoprotein (TAG)-72 to sterically unilamella liposomes. The cationic immunoliposomes are composed of cationic lipid (O,O'-dimyristyl-N-lysyl aspartate, DMKD), cholesterol, and 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-[maleimide(polyethyleneglycol)$_{2000}$] (DPPE-PEG-maleimide) with a molar ratio of 0.5:0.47:0.03. Plasmid DNA was effectively condensed by addition of transferrin (Tf) during the formation of anti-TAG-72 PEG-immunolipoplexes (PILPs). These anti-TAG-72 PILPs were able to adhere to the surface of TAG-72-overexepressing LS174T human colon cancer cells more effectively than conventional liposomes, thereby facilitating gene delivery in vitro. Furthermore, intravenous administration of the anti-TAG-72 PILPs into the tumor-carrying mice exhibited efficient localization of the reporter gene in the tumor tissues.

• 대한의용생체공학회:의공학회지
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• 제30권1호
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• pp.10-17
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• 2009

Accurate electroencephalography (EEG) forward calculation is of importance for the accurate estimation of neuronal electrical sources. Conventional studies concerning the EEG forward problems have investigated various factors influencing the forward solution accuracy, e.g. tissue conductivity values in head compartments, anisotropic conductivity distribution of a head model, tessellation patterns of boundary element models, the number of elements used for boundary/finite element method (BEM/FEM), and so on. In the present paper, we investigated the influence of modeling errors in the boundary element volume conductor models upon the accuracy of the EEG forward solutions. From our simulation results, we could confirm that accurate construction of boundary element models is one of the key factors in obtaining accurate EEG forward solutions from BEM. Among three boundaries (scalp, outer skull, and inner skull boundary), the solution errors originated from the modeling error in the scalp boundary were most significant. We found that the nonuniform error distribution on the scalp surface is closely related to the electrode configuration and the error distributions on the outer and inner skull boundaries have statistically meaningful similarity to the curvature distributions of the boundary surfaces. Our simulation results also demonstrated that the accumulation of small modeling errors could lead to considerable errors in the EEG source localization. It is expected that our finding can be a useful reference in generating boundary element head models.

• Applied Microscopy
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• 제28권3호
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• pp.399-414
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• 1998

Glandular trichomes present on the leaf surface of Drosera capensis were examined using transmission electron microscopy. A large number of stalked glands exist on the adaxial surfaces of the leaf blade. The secretory head is composed of two layers of secretory cells, one layer of middle cells, and the inner tracheids. The secretory cells contain rough endoplasmic reticulum, mitochondria, plastids, Golgi apparatus, and vacuoles. The secretory cells show prominent cell wall ingrowth, and thick cuticle restricted on the subcuticular wall. Frequently, the cuticle has some pores, canal-like structures, showing electron -dense granules being penetrated through them. Ultrastructural localization using diaminobenzidine showed the electron-dense deposits in the vacuole. No peroxidase activity was seen in the cell wall and cytolasm. The activity of peroxidase (POX) isozymes in Drosera which isoelectric point (pI) is 3.6 and some anionic POX isozymes which pIs are laid between 3.6 and 4.6 were especially increased according to the development and the formation of glandular trichomes. Also, the activity of some POX isozymes which isoelectric points are laid between 4.6 and 5.1 were increased in the regions of leaves which has trichomes.

• Journal of Korean Neurosurgical Society
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• 제37권3호
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• pp.197-200
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• 2005

Objective: Ultrasound can be used in the treatment of large intracerebral hematoma. The authors present our experiences with Ultrasound-guided catheter placement for lysis and drainage of ganglionic hematoma, with emphasis on technical aspects. Methods: The authors applied real-time ultrasonography for the aspiration of intracerebral hematoma in 6cases. Ultrasound-guided aspiration via a burrhole was performed under local anesthesia. We selected a temporal entry point instead of the frequently used precoronal approach in ganglionic hematoma. A burrhole was made 4 to 6cm posterior from posterior border of frontal process of the zygomatic bone at the level of 4 to 5cm above the external auditory meatus. Results: In all patients, the catheter was placed accurately into the hematoma target. All patients were irrigated with urokinase once to three times a day. The catheter could be removed within two or three days. The mean hematoma volume was reduced from initially 32mL to 5mL in an average of two days. There were no intraoperative complications related to the use of real-time ultrasonography and no postoperative infections were noted. Conclusion: Ultrasound allows an easy and precise localization of the hematoma and the distance from the surface to the target can be calculated. Ultrasound-guided catheter placement for fibrinolysis and hematoma drainage is a simple and safe procedure.