• IMMUNE NETWORK
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• v.7 no.2
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• pp.57-65
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• 2007

Background: Cordyceps militaris have been reported to modify the immune and inflammatory responses both in vivo and in vitro. Macrophages play important roles in the innate immunity through the phagocytosis of antigens. This study examined the effects of Cordyceps militaris on the activation of murine macrophage RAW 264.7 cells and primary macrophages. Methods: The components contained in culture broth of Cordyceps militaris were purified by propyl alcohol extraction and HP 20 column chromatography to CMDB, CMDBW, CMDB5P, and CMDB25P. The amounts of nitric oxide (NO) were determined by using ELISA, Griess reagent respectively. The amounts of some cytokines were determined by using ELISA, western blot, and RT-PCR The expression levels of cell surface molecules (ICAM-1, B7-1 and B7-2) were measured by flow cytometric analysis. Results: All the components of Cordyceps militaris produced significant amounts of NO. In particular, CMDB produced much more NO in RAW 264.7 cells and primary macrophages than other fractions of Cordyceps militaris. CMDB increased significantly the production of tumor necrosis factor (TNF)-${\alpha}$, interleukin (IL)-1${\beta}$, and IL-6 dose-dependently in RAW 264.7 cells. Examination of the gene expression level also showed that the enhanced production of cytokines was correlated with the up-regulation of i-NOS expression, cycloxygenase (COX)-2 expression, IL-1${\beta}$ and IL-6 expression, and TNF-${\alpha}$ expression on the expression of mRNAs by semi-quantitative RT-PCR Western blot analysis also confirmed that CMDB enhances the expression level of these cytokines. Conclusion: These results show that CMDB stimulates the production of NO and pro-inflammatory cytokines and can also up-regulate the gene expression levels in macrophages.

• Journal of Korean Society of Forest Science
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• v.96 no.3
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• pp.251-258
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• 2007

Airborne Lidar (light detection and ranging) can be an effective alternative in forest inventory to overcome the limitations of conventional field survey and aerial photo interpretation. In this study, we attempt to develop methodologies to identify individual trees and to estimate tree height from airborne Lidar data. Initially, digital elevation model (DEM) data representing the exact ground surface were generated by removing non-ground returns from the multiple-return laser point clouds, obtained over the coniferous forest site of rugged terrain. Based on the canopy height model (CHM) data representing non-ground layer, individual tree heights are extracted through pseudo-grid method and moving window filtering algorithm. Comparing with field survey data and aerial photo interpretation on sample plots, the number of trees extracted from Lidar data show over 90% accuracy and tree heights were underestimated within 1.1m in average at two plantation stands of pine (Pinus koraiensis) and larch (Larix leptolepis).

• Journal of Korean Society for Geospatial Information Science
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• v.13 no.1 s.31
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• pp.19-25
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• 2005

Nowadays, it is possible to construct the DEMs of urban area effectively and economically by LiDAR system. But the data from LiDAR system has form of DSM which is included various objects as trees and buildings. So the preprocess is necessary to extract the DEMs from LiDAR DSMs for particular purpose as effects analysis of man-made objects for flood prediction. As this study is for extracting DEM from LiDAR DSM of urban area, we detected the edges of various objects using edge detecting algorithm of image process. And, we tried mean value filtering, median value filtering and minimum value filtering or detected edges instead of interpolation method which is used in the previous study and could be modified the source data. it could minimize the modification of source data, and the extracting process of DEMs from DSMs could be simplified and automated.

• Asian-Australasian Journal of Animal Sciences
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• v.15 no.8
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• pp.1165-1169
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• 2002

Palm kernel cake (PKC), a by-product of oil palm seeds after extraction of their oil. The aim of this study was to investigate the effects of different levels of PKC on growth performance and blood lipids in rats. A total of 64 Sprague-Dawley (8 weeks of age) male rats were assigned individually to four treatments with different levels of PKC in the diet: 0, 15, 20 and 25%. No differences (p<0.05) were found in daily feed intake (6-8 g/day), body weight, growth rate and epididymal fat weight for all the dietary groups. Plasma protein and very low density lipoprotein (VLDL) triacylglycerol (TG) were higher (p<0.05) for 20% PKC fed rats than the control rats. Conversely, the plasma cholesterol and TG and VLDL-phospholipid (PL) concentrations of the control rats were higher (p<0.05) than those of PKC fed rats. The VLDL-protein, total cholesterol, free cholesterol (FC) and cholesteryl ester (CE) were not significantly different (p>0.05) among the treatment groups. Rats fed PKC had greater (p<0.05) ratios of total surface to core lipid components [(FC+PL)/(CE+TG)] than control rats. The results reflect dissimilarities of VLDL particle size between PKC treatment and control rats, where the plasma of the PKC treated rats contained more lipid rich VLDL. In conclusion, there was no adverse effect on growth performance when inclusion of PKC up to 25%. However, fibre content may affect the plasma lipid concentrations.

• Journal of the Korea Organic Resources Recycling Association
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• v.4 no.2
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• pp.47-53
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• 1996

This study was carried out to estimate the amount and the characteristics of sewage sludge disposed of in the Nanjido sludge landfill site. Boring test was conducted to estimated the landfill volume and to get sludge samples to be analyzed. Total analysis and leaching test were performed to find the characteristics of sewage sludge. The Nanjido sludge landfill site had the surface area of $50,000m^2$ and the average depth of 15m. The estimated sludge volume was 600,000ton. Sewage sludge in Nanjido sludge landfill site was very stable. Results of total analysis of heavy metals indicated that the sludge could be classified as a non-hazardous waste according to the California state regulation. The sludge also could be used as compost based on compost quality criteria of foreign countries. Results of Korea Extraction Procedure showed that sewage sludge in Nanjido sludge landfill site was not a hazardous waste.

• Journal of Korean Society on Water Environment
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• v.28 no.6
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• pp.843-850
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• 2012

Due to the fast response to algae bloom issue in drinking water treatment plant, very fast determination methodology for algal toxin is required. In this study, column switching technique based online preconcentration method was combined with high resolution full scan mass spectrometer to save sample preparation time and to obtain fast and accurate result. After parameter optimization of online preconcentration, 1mL filtered sample was directly injected to trap column with switching valve system. Next, target toxins are eluted by 98% acetonitrile and analysed with 150 - 1,100 amu scan range at 50,000 resolving power. Method detection limit (MDL) for microcystin-LR, the most toxic isomer, was 0.1 ng/mL and others such as microcystin-YR, microcystin-RR and nodularin were 0.08, 0.03 and 0.04 ng/mL, respectively. This is the best improved sensitivities with 1mL volume in the literature. Furthermore, due to the use of ultra pressure HPLC (UPLC), the whole method run was completed in 4 min. Real sample applications for 173 sample including 55 surface water and 118 treatment plant samples for raw and treated water could be done within 16 hours. In our calculation, this methodology is roughly 80% faster than the previous manual solid-phase extraction with LC-MS/MS method.

• Transactions of the Korean Society for Noise and Vibration Engineering
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• v.24 no.12
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• pp.985-991
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• 2014

The combined method utilizing airborne LiDAR and GIS data is suggested to extract 3-dimensional hybrid city model including roads and buildings. Combining the two types of data is more efficient to estimate the elevations of various types of roads and buildings than using either LiDAR or GIS data only. This method is particularly useful to model the overlapped roads around the so called spaghetti junction. The preliminary model is constructed from the LiDAR data, which can give wrong information around the overlapped parts. And then, the erratic vertex points are detected by imposing maximum vertical grade allowable on the elevated roads. For the purpose of efficiency, the erratic vertex points are corrected through linear interpolation method. To avoid the erratic treatment of the LiDAR data on the facades of buildings 2 meter inner-buffer zone is proposed to efficiently estimate the height of a building. It is validated by the mean value(=5.26 %) of differences between estimated elevations on 2 m inner buffer zone and randomly observed building elevations.

• Journal of Conservation Science
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• v.29 no.4
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• pp.421-435
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• 2013

The purpose of this study is to suggest environmental guidelines for the conservation of ceramics excavated from underwater. Accordingly, the conditional change of the soluble salt on surface of the excavated ceramics was examined by changing the relative humidity. Examining the relative ratio [high humidity(RH70%+RH80%)/low humidity(RH20%+ RH40%)] for 24 weeks using accumulated conductivity(${\mu}s/cm$), the result showed that the amount of ion elution increased more in high humidity than in low humidity. In particular, the ion elution increased significantly within the celadon sample. In addition, comparing the accumulated conductivity and physical characteristics of the samples in high humidity, the results indicated that the amount of the ion elution is proportioned to the increased rate of the sample's absorption capacity and porosity. Ceramics excavated from underwater has risks of the secondary physical and chemical attacks from remaining salts. Therefore, it is suggested these ceramics be stored in a storage which maintains proper temperature and low humidity conditions. Also, the collections need to be pre-classified according to the properties of the materials.

• Journal of Korean Society of Coastal and Ocean Engineers
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• v.26 no.2
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• pp.103-111
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• 2014

The wave power, extracted from a circular array of small power buoys, is investigated under the potential theory. It is assumed that the buoy's radius, the draft, and the separation distance are much smaller than the water depth, the wave length, and the radius of a circular deployment area. The boundary value problem involving the macro-scale boundary condition on the mean surface covered by buoys is solved using the eigenfunction expansion method. The capture width, which is defined as the ratio of the extracted power to the wave power per unit length of the incident wave crest, is assessed for various combinations of packing ratio, radius of a circular array, and PTO damping coefficient. It is found that the circular array deployment is more effective in the viewpoint of efficiency than the single large buoy of the same total displaced volume.

• Economic and Environmental Geology
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• v.29 no.4
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• pp.495-508
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• 1996

This study had three purposes: (1) to investigate dispersion and enrichment level of potentially toxic elements; (2) to identify uranium-bearing minerals in black shales; and (3) to assess the chemical speciation of heavy metals in soils and sediments. Rock, surface soil and stream sediment samples were collected in the Chungjoo area covered with black shales in Korea. These samples were analyzed for multi-elements using INAA and ICP-AES. The maximum abundance of U in black shales is 56 ppm and radioactivity counts up to 240CPM. Molybdenum, V, Ba, Cu, and Pb are enriched in black shales and most of soils show high concentrations of U, Mo, Ba, Cu, Pb and Zn. Concentrations of potentially toxic elements decrease in the order of mountain soil > farmland soil > paddy soil. Enrichment index of soils and sediments are calculated and higher than 1.0 in the black shale area with the highest value of 6.1. In order to identify U-bearing minerals, electron probe micro analysis was applied, and uraninite and brannerite in black shale were found. Uraninite grains are closely associated with monazite or pyrite with the size of $2{\mu}m$ to $10{\mu}m$ in diameter whereas brannerite occurs as $50{\mu}m$ euhedral grains. With the results of sequential extraction scheme, residual fractions of Cu, Pb and Zn in soils are mainly derived from weathering of black shale but Cu, Pb and Zn in sediments are present as non-residual fractions. Lead is predominantly present as oxidizable phase in soils whereas Zn is in exchageable/water-acid soluble phase in sediments.