• Title/Summary/Keyword: surface exposure

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UVB-INDUCED CHANGES OF BARRIERFUNCTION AND MORPHOLOGY OF THE HAIRLESS MOUSE SKIN (자외선-B에의한 Hairless mouse의 보호기능과 표면구조의 변화 영향)

  • Kim, Jin-Jun;Park, Mun-Eok;Gang, Se-Hun
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.18 no.1
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    • pp.81-98
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    • 1992
  • Hairless mice (Skh:HR-1) exposed to single doses (0.5, 1.0 and 3. OMED) of UV-B radiation were displayed remarkable changes of barrier function and surface morphology. Trans- epidermal water loss (TEWL) as an index of barrier function was measured by evaporimeter, and wrinkle density (WD) as an index of morphological alteration was measured by image analyzer. Significant changes of TEWL were not observed in the control and 0. SMED group, but 1.OMED and 3. OMED groups noted significant difference. TEWL of 3. OMED group was rapidly increased to the 3rd day and decreased until the 14th day when it reached nearly to normal level, Time-courses of TBWL for 1. OMEB and 3. OMED groups displayed similar pattern, but different only in the magnitude. WD were significantly decreased during the 3rd-5th day in all of the irradiated groups and then increased during the last period to the 14th day, but did not recover the normal level at the 14th day. Time-courses of WD for all groups exhibited similarity, and were entirely dependent on the exposed doses. We also observed histological changes which included hyperplasia, sunburn cell (SBC) formation, accumulation of polymorphonuclear leukocyte (PMNs), and loss of collagen of UVB- exposed hairless mouse skin. Changes of TEWL and WD are helpful in understanding of epidermal and dermal damages by single exposure of UVB.

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Assessment of Flood Vulnerability to Climate Change Using Fuzzy Model and GIS in Seoul (퍼지모형과 GIS를 활용한 기후변화 홍수취약성 평가 - 서울시 사례를 중심으로 -)

  • Kang, Jung-Eun;Lee, Moung-Jin
    • Journal of the Korean Association of Geographic Information Studies
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    • v.15 no.3
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    • pp.119-136
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    • 2012
  • The goal of this study is to apply the IPCC(Intergovernmental Panel on Climate Change) concept of vulnerability to climate change and verify the use of a combination of vulnerability index and fuzzy logic to flood vulnerability analysis and mapping in Seoul using GIS. In order to achieve this goal, this study identified indicators influencing floods based on literature review. We include indicators of exposure to climate(daily max rainfall, days of 80mm over), sensitivity(slope, geological, average DEM, impermeability layer, topography and drainage), and adaptive capacity(retarding basin and green-infra). Also, this research used fuzzy model for aggregating indicators, and utilized frequency ratio to decide fuzzy membership values. Results show that the number of days of precipitation above 80mm, the distance from river and impervious surface have comparatively strong influence on flood damage. Furthermore, when precipitation is over 269mm, areas with scare flood mitigation capacities, industrial land use, elevation of 16~20m, within 50m distance from rivers are quite vulnerable to floods. Yeongdeungpo-gu, Yongsan-gu, Mapo-gu include comparatively large vulnerable areas. This study improved previous flood vulnerability assessment methodology by adopting fuzzy model. Also, vulnerability map provides meaningful information for decision makers regarding priority areas for implementing flood mitigation policies.

The effect of irradiated cancellous human bone on exophytic bone formation in residual ridge of the beagle dog (방사선 조사 망상골이 외방형 골형성에 미치는 영향)

  • Chung, Mi-Hyun;Herr, Yeek;Kwon, Young-Hyuk;Park, Joon-Bong;Chung, Jong-Hyuk
    • Journal of Periodontal and Implant Science
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    • v.37 no.4
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    • pp.791-803
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    • 2007
  • The purpose of this study was to evaluate exophytically vertical bone formation in residual ridge of the beagle dog by the concept of guided bone regeneration with a titanium reinforced e-PTFE membrane combined with irradiated cancellous human bone. Twelve male beagle dogs(mean age 1.5 years and mean weight 12kg) were used for this study. The alveolar ridges after extraction of all mandibular premolars were surgically and horizontally removed. At 8 weeks after extractions, full-thickness flap was reflected and cortical bone was removed with round bur and copious irrigation. Rectangular parallelepiped(10mm in length, 5mm in width, and 4mm in height) bended with titanium-reinforced e-PTFE(TR e-PTFE) membrane was placed on the decorticated alveolar ridge, fixed with metal pins and covered with full-thickness flap and assigned as a control group. Test groups ere treated with TR e-PTFE membrane filled with irradiated cancellous human bone. Of twelve beagle dogs, four control dogs and four test dogs without membrane exposure to oral cavity were sacrificed at 8 and 16 weeks respectively. The surgical sites were dissected out, fixed in 4% buffered formaldehyde, dyed using a Villanueva staining technique, and processed for embedding in plastic resin. The cutting and grinding methods were routinely processed for histologic and histomophometric analyis of exophytic bone formation as well as statistical analysis. The results of this study were as follows: 1. Exophytic bone formation in the both of experimental groups was increased respectively after surgery from 23.40% at 8 weeks to 46.26% at 16 weeks in the control groups, from 40.23% at 8 weeks to 47.11% at 16 weeks in the test groups(p<0.05). 2. At 8 weeks after surgery, exophytic bone formation was made 40.23% in the test groups and 33.40% in the control groups. Exophytic bone formation was significantly made in the test group more than in the control group. At 16 weeks after surgery, exophytic bone formation was made 44.11% in the test groups and 46.26% in the control groups. Exophytic bone formation was made in the test groups more than in the control groups, but there was no statistically significant differences. 3. The membrane was fixed with metal pins to closely contact it to the bone surface. So, collapse and deviation of the membrane could be prevented and in growth of connective tissue also could be blocked from the periphery of the membrane. On the basis of these findings, wee suggest that intraoral experimental model for exophytic bone formation may be effective to evaluate the effect of bone graft material. And it indicates that combined use of membrane and ICB graft material is more effective than use of membrane only for exophytic bone formation.

An Experimental Study on the Influence of Masonry InFilled Walls on the Seismic Performance of Reinforced Concrete Frames with Non-seismic Details (정적실험을 통한 조적채움벽체가 비내진상세 RC 골조의 내진성능에 미치는 영향 평가)

  • Kim, Kyoung-Min;Choen, Ju-Hyun;Baek, Eun-Rim;Oh, Sang-Hoon;Hwang, Cheol-Seong
    • Journal of the Korea institute for structural maintenance and inspection
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    • v.21 no.3
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    • pp.114-120
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    • 2017
  • In this paper, the effect of the masonry infill walls on the seismic performance of the reinforced concrete(RC) frames with non-seismic details was evaluated through the static test of an masonry infilled RC frame sub-assemblage with non-seismic details of real size, and comparison with the test results of the RC frame sub-assemblage with non-seismic details. As the test results, lots of cracks occurred on the surface of the entire frame due to the compression of the masonry infilled wall, and the beam-column joint finally collapsed with the expansion of the shear crack and buckling(exposure) of the reinforcement. On the other hand, the stiffness of the shear force-story drift relationship decreased due to the wall sliding crack and column flexural cracks, and the strength finally decreased by around 60% of the maximum strength. The damage that concentrated on the upper and lower parts of columns was dispersed in the entire frame such as columns, a beam, and beam-column joints due to the wall, and the specimen was finally collapsed by expansion of the shear crack of the joint, not the shear crack of the column. Also, the stiffness of RC frame increased by 12.42 times and the yield strength by 3.63 times, while the story drift at maximum strength decreased by 0.18 times.

A Microscope Study of Fluoride Effects on the Rat Incisor Enamel Formation (흰쥐 절치의 법랑질 형성에 미치는 불소의 효과에 관한 현미경적 연구)

  • Lim, Do-Seon;Chang, Byung-Soo;Jeong, Je-O;Jeong, Soon-Jeong;Jeong, Moon-Jin
    • Applied Microscopy
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    • v.34 no.2
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    • pp.145-157
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    • 2004
  • The aim of the present study was to examine in detail, both at light and electron microscopical levels, the morphological variations in ameloblast of the fetal rat incisor enamel organ. Rats were started on distilled water at the beginning of pregnancy. The pups were sacrificed 11 days after delivery and animals were perfused intravascularly with glutaraldehyde and the incisors were removed. To examine on the ultrastructure of the ameloblast, the study employed primary light microscopy but electron microscopy was used to clarify some of the light microscopic finding. Longitudinal sections through the incisors of the rat show a continuous layer of ameloblasts on the labial surface of the tooth. This layer contains the entire sequence of developmental stages in enamel production. The ameloblast layer was divided into three main zones: 1) Presecretory zone, region of ameloblasts facing pulp. 2) Secretory zone, region of inner and outer enamel secretion. 3) Maturation zone, region of reduced ameloblasts. In particularly, the present study has shown that two distinctively different types of ameloblasts appear in the enamel organ during enamel maturation in the rat incisor. These two types have been designated ruffle-ended ameloblasts (rAB) and smooth-ended ameloblasts (sAB). The fluoride produces marked alteration in the fine structure of ameloblast from teeth of young rats, such as large confluent distensions of the endoplasmic reticulum and swelling of isolated mitochondria. This experimental data suggested that exposure prolonged of animal to high level of fluoride appears to induce a few dramatic changes in the normal appositional growth and initial mineralization of enamel created during amelogenesis.

Analysis of toluene diisocyanate of adhesives in food contact materials by GC/MS (GC/MS에 의한 식품 포장재에 사용되는 접착제의 Toluene diisocyanate 분석)

  • Oh, Chang-Hwan;Kim, Ji-Young;Jo, Cheon-Ho;Park, Heera;Kwun, Ki-Sung;Kim, Meehye;Myung, Seung-Woon
    • Analytical Science and Technology
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    • v.18 no.6
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    • pp.511-519
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    • 2005
  • A method for determination of toluene diisocyanates (TDI) in toluene diisocynate (TDI)-based polyurethane (PUR) packing material was investigated, and also the migration of TDI to food was studied. TDI was extracted using food simulants such as n-haptane and 4% aqueous acetic acid. The determinations were performed using gas chromatography/mass spectrometry (GC/MS). One of major components for polyurethane, toluene diisocyanates, were detected in ten samples among twenty six food contact materials with the concentration range of $0.51{\sim}60.88{\mu}g/ml$. However the highest extracted amount was just 0.7% of $60.88{\mu}g/ml$ if the contact surface of food packing for extracting liquid was limited to the outer layer without exposure of the cutting edge of food packing multi-layers. The result of this study and the analysis method for TDI diisocyanate will be very useful for further study about food contact material, and the monitoring result could be used for evaluating the safety of food contact material before it is to be used for food, preservation.

Transgastric Endoscopic Cholecystectomy in a Dog : Natural Orifice Transluminal Endoscopic Surgery (개에서 내시경을 이용한 경위장관 담낭절제술 1예 : 자연개구부 내시경수술)

  • Jeong, Seong-Mok;Kim, Young-Ill;Lee, Jae-Yeon;Jee, Hyun-Chul;Park, Ji-Young;Park, Jong-Heon;Kim, Ji-Yeon;Lee, Sang-Il;Kim, Myung-Cheol;Shin, Sang-Tae;Lee, Young-Won
    • Journal of Veterinary Clinics
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    • v.24 no.3
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    • pp.315-319
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    • 2007
  • Transgastric endoscopic cholecystectomy was successfully accomplished in a 1-year-old, 15 kg, female, mongrel dog. Single-working channel flexible gastric endoscope was used with the aid of one abdominal laparoscopic port. Gastrotomy was performed using endoscopic needle knife at the ventral antral region. Through the gastric incision endoscope was advanced and retroflexed for the visualization of gallbladder. For the better exposure of surgical field, gentle traction was applied at the fundus of the gallbladder using laparoscopic grasping forceps. Cystic duct and artery was ligated using endoclips. After transecting the duct and artery, gallbladder was dissected using endoscopic coagulating grasping forceps and needle knife. Resected gallbladder was retrieved through the mouth and gastric incision site was sutured using endoclips. There was no evidence of bile leakage or stomach leakage on postoperative day (POD) 3. On POD 16, gastric endoscopy and laparoscopy was performed. Gastric endoscopy revealed complete adhesion of incision site. The content of the peritoneum appeared healthy, with no sign of infection, bile staining, or organ injury. The omentum was adhered over resected gallbladder fossa and the serosal surface of gastrotomy site. This is the first report of NOTES cholecystectomy in the dog and provides new concept of cholecystectomy of the dog.

Effect of Turbidity Changes on Tissues of Zacco koreanus (탁도 변화가 참갈겨니 (Zacco koreanus) 조직에 미치는 영향)

  • Shin, Myung-Ja;Kim, Jeong-Sook;Hwang, Yun-Hee;Lee, Jong-Eun;Seo, Eul-Won
    • Korean Journal of Ecology and Environment
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    • v.41 no.1
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    • pp.73-80
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    • 2008
  • Present study aims to investigate the effect of muddy water on the gill and the kidney tissues of Zacco koreanus under high turbidity rearing condition. The gill of Z. koreanus showed abnormal shapes in its secondary lamellae and a rough surface with impure debris in the high level of turbidity and the longer raising period condition. In addition, the gills showed the edema, the exfoliation of epithelial cell, and the fusion of the secondary lamellae. In case of kidney tissue, the atrophied glomerulus was observed, and the empty space in Bowman's capsule was wider. The SOD activities in both gill and kidney tissues were increased in proportion to the high level of turbidity. On the while, CAT and GPX activities were shown constant level in the gill, but were increased in the kidney in the high turbid muddy water. These results indicate that the harmful radicals which generate by high level of turbidity could be removed partly by antioxidant enzymes in the kidney. The concentrations of micro heavy metal ions accumulated in the gill increased drastically at the 1,000 NTU. Based on the above results, it is considered that the exposure to the high level of turbidity for long period may affect on the structures of tissues, and change the enzymatic balance in Z. koreanus, causing the fatal disease.

Shear bond strength between universal adhesives with various pH and dual-cured resin cements (다양한 pH를 가지는 Universal adhesives와 이원 중합 레진 세멘트 간의 전단결합강도)

  • Kwon, Sung-Joo;Park, Jeong-Kil;Son, Sung-Ae
    • Korean Journal of Dental Materials
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    • v.45 no.4
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    • pp.301-310
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    • 2018
  • The purpose of this study is to evaluate the compatibility of universal adhesives with two dual-cured resin cements. Eighty human molars were divided into eight groups. Tooth was embedded in self-curing acrylic resin and sectioned horizontally to exposure dentine surface. After polishing with 600-grit SiC paper, adhesives were applied. All-Bond Universal (Bisco), G-premio bond (GC), Scotch-bond universal (3M ESPE), Scotch-bond Multipurpose (3M ESPE) were used in this study. Calibra (Dentsply) as a conventional dual-curing resin cement and RelyX Ultimate (3M ESPE) as an amine free resin cement were used. The adhesives and the cements were applied according to the manufacturer's instructions. Final specimens were cylinder (diameter 2mm, height 3mm) shape. After storing in distilled water at $37^{\circ}C$ for 7 days the shear bond strength (SBS) test was performed. There was no significant difference in shear bond strength between the adhesives when RelyX Ultimate was used (p>0.05). However, when Calibra used with Scotch-bond Multipurpose and All-Bond Universal were used, statistically higher SBS was observed, as compared to the groups which Calibra cements with G-premio bond and Scotch-bond universal adhesive (p<0.05) were used. Within the limitations of this study, RelyX Ultimate resin cement was compatible with universal adhesives of various pH. All-Bond Universal adhesive was compatible with a resin cement containing the tertiary amine.

Effects of Dry Roasting on the Vitamin E Content and Microstructure of Peanut (Arachis hypogaea)

  • Eitenmiller, Ronald R;Choi, Sung-Gil;Chun, Jiyeon
    • Journal of agriculture & life science
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    • v.45 no.4
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    • pp.121-133
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    • 2011
  • Effects of roasting on vitamin E content, color, microstructure and moisture of peanuts, and vitamin E content in peanut oils prepared from the roasted peanuts were investigated. Runner-type peanuts were roasted at 140, 150, and $160^{\circ}C$ for 10-20 min. As roasting temperature and time increased, the CIELAB $L^*$ value of peanuts decreased while $a^*$ and $b^*$ values increased, resulting in formation of the golden brown color of roasted peanuts. Moisture ratio (M/Mo) and color $b^*$ value of peanuts roasted at 140 to $160^{\circ}C$ showed a correlation of $b^*=21.61\;(M/Mo)^2-40.62\;(M/Mo)+34.12$ ($R^2=0.9123$). Overall changes in the tocopherol contents of peanuts and peanut oils were significantly affected by roasting temperature and time (p<0.05). Roasting at $140^{\circ}C$ caused a slight increase in the levels of tocopherols of peanuts over roasting time up to 20 min (p<0.05). There was no significant change in the tocopherol levels of peanuts during roasting at $150^{\circ}C$ for 20 min (p>0.05). At $160^{\circ}C$, the levels of tocopherols significantly decreased during the initial 10 min of roasting (p<0.05) while there was no extended loss after 10 min, resulting in about 5, 12, 20, and 10% losses of ${\alpha}$-, ${\beta}$-, ${\gamma}$- and ${\delta}$-T, respectively. After 20 min, total tocopherols decreased by 18%. However, tocopherol contents of pressed peanut oils significantly decreased at all roasting temperatures (p<0.05). After roasting peanuts at $160^{\circ}C$ for 20 min, about 84% of initial ${\alpha}$-T in peanut oils was retained. ${\alpha}$-T was the most stable to roasting while ${\gamma}$-T was the least. Swollen epidermal cells on the inner surface and broken cell walls of parenchyma tissue of peanut cotyledon were observed in peanuts after roasting at $160^{\circ}C$ for 15 min. Severe changes in microstructure of peanut by roasting would contribute to vitamin E stability because of exposure of oil droplets in peanuts to oxygen.