• Archives of Plastic Surgery
• /
• v.33 no.1
• /
• pp.46-52
• /
• 2006

High-density micromass culture was needed to take three dimensions culture with ASCs(adipose derived stromal cells) and chondrogenesis. However, the synthetic polymer has hydrophobic character and low affinity to cells and other biomolecules. Therefore, the surface modification without changes of physical and chemical properties is necessary for more suitable condition to cells and biomolecules. This study was performed to investigate the effect of surface modification of poly (lactic-co-glycolic acid)(PLGA) scaffold by plasma treatment (P(+)) on the adhesion, proliferation and chondrogenesis of ASCs, and not plasma treatment (P(-)). ASCs were isolated from human subcutaneous adipose tissue obtained by lipectomy and liposuction. At 1 hour 30 minutes and 3days after cell seeding onto the P(-) group and the P(+) group, total DNA amount of attached and proliferated ASCs markedly increased in the P(+) group (p < 0.05). The changes of the actin under confocal microscope were done for evaluation of cellular affinity, at 1 hour 30 minutes, the shape of the cells was spherical form in all group. At 3rd day, the shape of the cells was fiber network form and finely arranged in P(+) group rather than in P(-) group. RT-PCR analysis of cartilage-specific type II collagen and link protein were expressed in 1, 2 weeks of induction. Amount of Glycoaminoglycan (GAG) markedly increased in P(+) group(p < 0.05). In a week, extracellular matrix was not observed in the Alcian blue and Safranin O staining. However in 2 weeks, it was observed that sulfated proteoglycan increased in P(+) group rather than in P(-) group. In conclusion, we recognized that plasma treatment of PLGA scaffold could increase the hydrophilic property of cells, and provide suitable environment for high-density micromass culture to chondrogenesis

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.22 no.4
• /
• pp.206-211
• /
• 2021

The purpose of this study is to understand the relationship between emotional labor, job burnout, and life satisfaction for art psychology counselors. The subjects were 225 art psychology counselors in ◯◯ City. As for the methods used, frequency analysis, reliability analysis, and correlation analysis were applied using SPSS 22.0, and multiple regression analysis was applied to causal relationships between variables. As a result of examining the effect on life satisfaction of emotional labor by art psychology counselors, surface behavior had a significant negative effect, while inner behavior also had a negative effect. Emotional exhaustion and a self-achievement decrease, which are sub-factors of job burnout for art psychology counselors, had a negative (-) effect on life satisfaction. This means that the lower the surface behavior of emotional labor by an art psychology counselor, the higher the inner behavior, and the lower the emotional exhaustion of job burnout and the lower the factor of self-achievement, the higher the life satisfaction. This study is considered to be basic data for the preparation of measures to reduce psychological burnout in art psychology counselors and can improve resilience in adaptation to the teaching profession.

• The Journal of the Convergence on Culture Technology
• /
• v.1 no.2
• /
• pp.91-95
• /
• 2015

The purpose of this study was to evaluate the difference voice production, according to the pelvic inclination while in the sitting position. Measure the sound produced(pitch) in three positions with the Praat program. position: anterior tilt position, posterior tilt position, neutral position(seat surface tilted 15 degrees). We found that the mean values of pitch were statistically significant different according to three types of sitting positions (p<0.05). The following result was observed: anterior tilt position > posterior tilt position > neutral position. There was significant difference in the neutral position. This finding suggests that the seat surface inclinations have an effect on speech production. Especially, neutral position may be an effective posture that may help increases the speech production.

• Journal of the Korean Society of Food Culture
• /
• v.27 no.6
• /
• pp.710-718
• /
• 2012

The purpose of this study was to investigate the antioxidative effects of Opuntia humifusa and determine the optimal mixing ratio of Opuntia humifusa powder and eggs for the preparation of pasta. In regard to its antioxidant effects, Opuntia humifusa powder had a total phenolic content and DPPH free radical scavenging activity of 6.64 mg/g and 90.12%, respectively. We used response surface methodology to obtain ten experimental points (including two replicates for Opuntia humifusa powder and egg) and Opuntia humifusa pasta formulation was optimized using rheology. Yellowness (p<0.001) displayed a linear model pattern, whereas lightness (p<0.01) and redness (p<0.01) were described by a quadratic model pattern. Mechanical properties showed significant values in hardness (p<0.5), chewiness (p<0.5), gummiess (p<0.5), and cohesiveness (p<0.5). The sensory evaluation parameters showed significant differences in color (p<0.5), appearance (p<0.5), flavor (p<0.5), texture (p<0.5) and overall quality (p<0.5). The optimum formulations processed by numerical and graphical optimization were found to be 7.30 g of Opuntia humifusa powder and 31.55 g of egg.

• Journal of the Korean Society of Food Culture
• /
• v.28 no.2
• /
• pp.204-211
• /
• 2013

The purpose of this study was to determine the optimal mixing conditions for two different amounts of turmeric (Curcuma longa L.) powder and olive oil for the processing of chicken sausage. The experiment was designed according to the central composite design of response surface methodology, with ten experimental points, including two replicates for turmeric powder and olive oil. The physicochemical and mechanical analysis of each sample, including water holding capacity, moisture content, lightness, redness, yellowness, hardness, chewiness, gumminess, and cohesiveness, showed significant differences. The results from sensory evaluations also showed very significant differences in color, flavor, tenderness, chewiness, and overall quality. The optimal formulation, calculated by numerical and graphical methods, was 1.89 g of turmeric powder and 9.77 g of olive oil. Under these conditions, the model predicted pH-6.01, salinity-0.20, WHC-94.88, $L^*$ value-61.13, $b^*$ value-37.45, hardness-$36.66{\times}10^2$ (N), springiness-8.70 (mm), chewiness-$26.88{\times}10^3$ ($N{\times}mm$).

• Journal of the Korean Society of Food Culture
• /
• v.32 no.1
• /
• pp.58-65
• /
• 2017

This study was conducted to optimize the conditions of a sweet rice paste as a replacement of red bean paste recipe using the central composite design of response surface methodology (RSM). Ten experimental recipes with two reference points (rice flour and sugar), were selected, and the physical and sensory characteristics of a sweet rice paste were measured. In the Rapid Visco Analyzer test, breakdown value was the highest in rice flour (105.73 RVU). Sweetness significantly increased with addition of sugar contents (p<0.0002) and viscosity increased with addition of rice flour contents (p<0.0185). Moisture contents decreased with increasing contents of rice flour and sugar, whereas yellowness and redness tend to increase. In the sensory evaluation test, quadratic models for color (p<0.01865), sweetness (p<0.0399), thickness (p<0.0073), and overall acceptability (p<0.0249) were approved as independent for the rice flour and sugar contents. In conclusion, the optimal mixing ratio of sweet rice paste was determined to be 45.38 g of rice flour and 77.95 g of sugar.

• Journal of Microbiology and Biotechnology
• /
• v.15 no.5
• /
• pp.930-937
• /
• 2005

The Plackett-Burman design and the response surface method (RSM) with a central composite design (CCD) were employed to develop a culture medium for ansamitocin P-3 production using Actinosynnema pretiosum ATCC 31565. Among the 11 nutrients tested using the Plackett-Burman design, two carbon sources, sucrose and dextrin, and two nitrogen sources, polypeptone and yeast extract, were selected. Optimization of the concentrations of the selected nutrients was then performed using RSM with CCD. After two rounds of RSM, the optimum concentrations ($\%w/v$) of sucrose, dextrin, polypeptone, and yeast extract were identified as 4.5, 4.5, 0.16, and 0.89, respectively. The maximum ansamitocin P-3 titer was 45.2 mg/l with the optimized medium, which was about 6 times higher than that (7.315 mg/l) obtained with an $R_{2}YE$ medium before optimization.

• International Journal of Advanced Culture Technology
• /
• v.8 no.3
• /
• pp.284-290
• /
• 2020

In this paper, we propose a method to generate and optimize the height map that is suitable to render a soap bubble. The height map represents the flow speed of soap bubbles. To this end, we have analyzed the flow of the soap bubble surface through experiment, derived the moving speed value for each section. Some image filters have been used for optimization that reflects the parameters of the derived height map. In addition, in order to verify the results of the study, actual data measuring the surface flow speed of soap bubbles, the speed of the initial height map, and the optimized height map speed have been compared and tested. Through this study, we reach the issue that it is possible to express the variable flow speed of soap bubbles with the optimized height map, and it will help to express various fluids.

• KSBB Journal
• /
• v.14 no.6
• /
• pp.737-741
• /
• 1999

Phenanthrene degrading bacteria were isolated from the petroleum contaminated soil near an oil tank. Four of 15 strains decreased surface tension of culture broth of phenanthrene-containing minimal media. H6, one of the isolated bacteria decreased surface tension of culture broth below 33 dyne/cm during growth on glucose. H6 was identified as Bacillus subtilis and biosurfactant produced by H6 was lipopeptide. The biosurfactant was produced at 0.13 g/L in the mineral medium containing 2% glucose. Critical micelle concentration(CMC) of the biosurfactant was 52 mg/L. Foaming power was similar to Tween 80 and dispersing power was superior to Tween 80m SDS and Brij30. High thermal stability and emulsion index were also observed.

• Journal of the Korean Society of Food Culture
• /
• v.29 no.1
• /
• pp.101-110
• /
• 2014

This study was performed to determine the optimal composition of a muffin added with jerusalem artichoke powder and oligosaccharide. The experiment was designed based on CCD (central composite design), and evaluation was carried out by means of RSM (response surface methodology), which included 10 experimental points with three replicates each for the two independent variables jerusalem artichoke powder and oligosaccharide. The experimental muffin was prepared according to a traditional recipe, except that the flour was partially replaced by jerusalem artichoke powder (5, 15, or 25%) and the sugar was partially replaced by oligosaccharide (25, 50, or 75%). Using F-test, height, moisture, a-value, b-value, springiness, cohesiveness, texture, and overall acceptability were expressed as a linear model, whereas volume, pH, L-value, appearance, flavor and taste were expressed as a quadratic model. Increased amounts of jerusalem artichoke powder led to reduction of sensory scores for appearance, flavor, taste, texture, and overall quality. The optimum formulation determined by the numerical and graphical methods were similar: jerusalem artichoke powder 10.99%, oligosaccharide 71.40%.