• Journal of Microbiology and Biotechnology
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• 제23권3호
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• pp.357-363
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• 2013

The identification of bacteriophage proteins on the surface of Lactobacillus rhamnosus Pen was performed by LC-MS/MS analysis. Among the identified proteins, we found a phage-derived major tail protein, two major head proteins, a portal protein, and a host specificity protein. Electron microscopy of a cell surface extract revealed the presence of phage particles in the analyzed samples. The partial sequence of genes encoding the major tail protein for all tested L. rhamnosus strains was determined with specific primers designed in this study. Next, RT-PCR analysis allowed detection of the expression of the major tail protein gene in L. rhamnosus strain Pen at all stages of bacterial growth. The transcription of genes encoding the major tail protein was also proved for other L. rhamnosus strains used in this study. The present work demonstrates the spontanous release of prophage-encoded particles by a commercial probiotic L. rhamnosus strain, which did not significantly affect the bacterial growth of the analyzed strain.

• 대한의용생체공학회:학술대회논문집
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• 대한의용생체공학회 1997년도 춘계학술대회
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• pp.161-162
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• 1997

It is very useful to perform the surgery simulation before implanting TAH(Total Artificial Heart} in a patient. The space of chest and the shape of vessels are different from patient to patient. So, It is desirable to customize a TAH design to the anatomy structure of a patient. Several studies are performed to visualize and explain the 3D structure of heart. These studies are performed using 2-dimensional ref or mated images and simple measurement. Anatomy structure of a human heart is not so simple. It is 4dimensional structure ; 3-dimensional plus time, heart beating. 3-dimensional reconstruction schemes of medical images developed for about 10 years are usually categorized into two types of rendering technique ; surface rendering and volume rendering. Volume rendering is preferable in medical image processing field because this technique can be applied without considering the complexity of geometry and change of field of interest. The usable space in the chest of patient can be measured by 3D volume matching of patient trunk and TAH model. This space changes with time. In this research we have developed the 4-dimensional volume match program of patient and TAH model. 3-dimensional rendered set of volumes along time were used to simulate TAH fitting trial. The quantitative measurement from this simulation could be applied to customize TAH design.

• 한국어류학회지
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• 제30권1호
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• pp.65-68
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• 2018

섬진자가사리 Liobagrus somjinensis 후각기관과 서식처 환경 및 생태적 습성의 연관성을 규명하기 위해 실체현미경과 디지털카메라를 이용하여 해부학적 특징을 묘사하였다. 후각기관은 주둥이 위에 좌우 한 쌍으로 존재하였으며, 전비공, 후비공, 비강으로 크게 구성되어 있었다. 전비공은 튜브형의 형태로 코수염과 윗입술 끝 사이에 위치하였다. 후비공은 표면과 평평한 유출공으로써 코수염의 기부 바로 아래에 위치해 있었다. 비강은 가로와 방사형으로 배열된 혀 모양의 22~24개의 판으로 구성된 로제트 구조를 보유하고 있었다. 결과적으로 섬진자가사리의 후각기관의 기능 해부학적 구조는 여울에서의 은신, 섭식, 야행성의 생활패턴에 연관된 높은 후각의존도를 반영하는 것으로 생각된다.

• 한국어류학회지
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• 제28권1호
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• pp.28-34
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• 2016

한국산 날개망둑 Favonigobius gymnauchen 후각기관은 2015년 7월과 8월 사이 전라북도 부안군 변산면 격포리의 조간대에서 채집된 개체들을 대상으로 실체현미경, 광학현미경 그리고 주사전자현미경을 이용하여 해부학 및 조직학적 특징들을 조사하였다. 후각기관은 두부 주둥이 위 좌우로 한 쌍이 존재하며, 전비공과 후비공, 한개의 비강, 두 개의 비낭, 후신경, 그리고 후구로 구성되었다. 비강 내 감각상피는 연결성 유형의 분포를 보였으며, 섬모성 감각세포만을 가지고 있었고, 감각세포, 지지세포, 기저세포들로 구성되었다. 비감각상피는 층상상피세포들로 구성되었으며, 표면에 많은 점액공을 가지고 있었다. 따라서 이러한 후각기관의 특징들은 연안지역 조수 웅덩이와 수심이 얕은 조간대의 생태적 서식처와 밀접한 관계가 있는 것으로 여겨진다.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제26권2호
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• pp.132-136
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• 2000

The cell surface glycoprotein CD44 is a kind of adhesion molecule, which binds hyaluronic acid, type I collagen and fibronectin. Although there have been numerous reports on the expression and the function of CD44 in lymphocytes and macrophages, very little is known about its distribution and definite role in epithelial tissue, especially in oral epithelial one. The present study was performed to investigate the distribution and expression of the CD44 in human gingiva and squamous cell carcinoma(SCC) arising in human gingiva. And the authors compared CD44 expression with histopathologic grade of SCC. The results were as follows: 1. The CD44 was strongly expressed in granular, spinous and basal layers of normal marginal and attached gingiva, in spinous and basal layers of normal sulcular gingiva, and in all epithelial layers of normal junctional gingiva. 2. In SCC of gingiva, the CD44 was expressed in all but one case. In most of the cases the CD44 was expressed at cell membrane and the degree of expression was relatively strong. 3. In low-grade SCC of gingiva, the CD44 was strongly expressed, especially at the basal and spinous layers of abundantly keratinized cancer nests. In high-grade SCC of gingiva, the CD44 expression tended to be weak but was strong at cells showing individual keratinization. This study suggest that the CD44 expression of normal and cancerous gingival epithelium is associated with the degree of proliferation and differentiation of epithelial cells.

• 대한한의학회지
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• 제26권1호
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• pp.11-17
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• 2005

This study was carried out to identify the components of the human heart meridian muscle, the regional muscle group being divided into outer, middle, and inner layers. The inner parts of the body surface were opened widely to demonstrate muscles, nerves, blood vessels and to expose the inner structure of the heart meridian muscle in the order of layers. We obtained the following results; $\cdot$ The heart meridian muscle is composed of muscles, nerves and blood vessels. $\cdot$ In human anatomy, the difference between terms is present (that is, between nerves or blood vessels which control the meridian muscle and those which pass near by). $\cdot$ The inner composition of the heart meridian muscle in the human arm is as follows: 1) Muscle H-l: latissimus dorsi muscle tendon, teres major muscle, coracobrachialis muscle H-2: biceps brachialis muscle, triceps brachialis muscle, brachialis muscle H-3: pronator teres muscle and brachialis muscle H-4: palmar carpal ligament and flexor ulnaris tendon H-5: palmar carpal ligament & flexor retinaculum, tissue between flexor carpi ulnaris tendon and flexor digitorum superficialis tendon, flexor digitorum profundus tendon H-6: palmar carpal ligament & flexor retinaculum, flexor carpi ulnaris tendon H-7: palmar carpal ligament & flexor retinaculum, tissue between flexor carpi ulnaris tendon and flexor digitorum superficial is tendon, flexor digitorum profundus tendon H-8: palmar aponeurosis, 4th lumbrical muscle, dorsal & palmar interrosseous muscle H-9: dorsal fascia, radiad of extensor digiti minimi tendon & extensor digitorum tendon 2) Blood vessel H-1: axillary artery, posterior circumflex humeral artery H-2: basilic vein, brachial artery H-3: basilic vein, inferior ulnar collateral artery, brachial artery H-4: ulnar artery H-5: ulnar artery H-6: ulnar artery H-7: ulnar artery H-8: palmar digital artery H-9: dorsal digital vein, the dorsal branch of palmar digital artery 3) Nerve H-1: medial antebrachial cutaneous nerve, median n., ulnar n., radial n., musculocutaneous n., axillary nerve H-2: median nerve, ulnar n., medial antebrachial cutaneous n., the branch of muscular cutaneous nerve H-3: median nerve, medial antebrachial cutaneous nerve H-4: medial antebrachial cutaneous nerve, ulnar nerve H-5: ulnar nerve H-6: ulnar nerve H-7: ulnar nerve H-8: superficial branch of ulnar nerve H-9: dorsal digital branch of ulnar nerve.

• 대한약침학회지
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• 제12권2호
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• pp.21-29
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• 2009

Objective : This study was carried to identify the anatomical component of FTMM(Foot Taeyang Meridian Muscle) in human lower limb, and further to help the accurate application to real acupuncture. Methods : FTM at the surface of the lower limb was labelled with latex. And cadaver was stripped off to demonstrate muscles, nerves and the others and to display the internal structures of FTMM, being divided into outer, middle, and inner layer. Results : FTMM in human lower limb is composed of muscles, nerves, ligaments etc. The internal composition of the FTMM in human lower limb are as follows : 1) Muscle : Gluteus maximus. biceps femoris, semitendinosus, gastrocnemius, triceps calf, fibularis brevis tendon, superior peroneal retinacula, calcaneofibular ligament, inferior extensor retinaculum, abductor digiti minimi, sheath of flexor tendon at outer layer, biceps femoris, semimembranosus, plantaris, soleus, posterior tibialis, fibularis brevis, extensor digitorum brevis, flexor digiti minimi at middle layer, and for the last time semimembranosus, adductor magnus, plantaris, popliteus, posterior tibialis, flexor hallucis longus, dorsal calcaneocuboidal ligament at inner layer. 2) Nerve : Inferior cluneal nerve, posterior femoral cutaneous n., sural cutaneous n., proper plantar branch of lateral plantar n. at outer layer, sciatic nerve, common peroneal n., medial sural cutaneous n., tibial n. at middle layer, and for the last time tibial nerve, flexor hallucis longus branch of tibial n. at inner layer. Conclusions : This study proves comparative differences from already established studies from the viewpoint of constituent elements of FTMM in the lower limb, and also in the aspect of substantial assay method. We can guess that there are conceptional differences between terms (that is, nerves which control muscles of FTMM and those which pass near by FTMM) in human anatomy.

• Journal of Nutrition and Health
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• 제56권6호
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• pp.655-666
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• 2023

Purpose: Gestational nutrition has an impact on the growth and development of the fetus. Choline (C) and docosahexaenoic acid (DHA) are important and essential nutrients for humans that play a role in the structural integrity of the membranes as well as signalling. C is used in the synthesis of phosphatidylcholine, and cell membranes are highly enriched with DHA. The dietary intake of C or DHA during pregnancy directly influences fetal development. Currently, there is no evidence to prove the effectiveness of the combined dietary supplementation of both C and DHA during gestation on developmental outcomes in the offspring. Methods: The current study was designed to assess the physical, sensory, and motor development of rat pups born to mothers supplemented with C and/or DHA during the entire gestational period. Pregnant rat dams were divided into the following five groups: Normal control (NC), Saline control (SC), Choline (C), DHA, and Choline+DHA (C+DHA). The NC dams did not receive any supplementation during the entire gestation period. The experimental groups were supplemented with Saline, C, and/or DHA, respectively, during the entire gestation (E0 to delivery). Results: Rat pups (n = 6/group) exposed to combined C and DHA showed significant improvement in birth weight, fur development, eye-opening as well as weight gain on the 7th, 14th, and 21st postnatal day and pinnae detachment (assessed from birth to postnatal day 21) when compared with age-matched NC, SC or C or DHA pups. Further, significant reflex responses were observed in visual placing and bar holding of pups exposed to both C and DHA, whereas the differences in surface righting, negative geotaxis, and grasping reflexes were not significant between the groups. Conclusion: Gestational supplementation of both C and DHA rather than either of them alone is better in enhancing developmental outcomes in rat pups.

• Anatomy and Cell Biology
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• 제56권2호
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• pp.259-267
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• 2023

Stature is an essential component of biological profile analysis since it determines an individual's physical identity. Long bone dimensions are generally used to estimate the stature of skeletal remains; however, non-long bones such as the sternum, cranium, and sacrum may be necessary for some forensic situations. This study aimed to generate a regression equation for stature estimation of the skeletal remains in the Thai population. Ten measurements of the sacrum were measured from 200 dry sacra. The results revealed that the maximum anterior breadth (MAB) provided the most accurate stature prediction model among males (correlation coefficient [r]=0.53), standard error of estimation (SEE=5.94 cm), and females (r=0.48, SEE=6.34 cm). For the multiple regression model, the best multiple regression models were stature equals 41.2+0.374 (right auricular surface height [RASH])+1.072 (anterior-posterior outer diameter of S₁ vertebra corpus [APOD])+0.256 (dorsal height [DH])+0.417 (transverse inner diameter of S₁ vertebra corpus [TranID])+0.2 (MAB) with a SEE of 6.42 cm for combined sex. For males, stature equals 63.639+0.478 (MAB)+0.299 (DH)+0.508 (APOD) with a SEE of 5.35, and stature equals 75.181+0.362 (MAB)+0.441 (RASH)+0.132 (maximum anterior height [MAH]) with a SEE of 5.88 cm for females. This study suggests that regression equations derived from the sacrum can be used to estimate the stature of the Thai population, especially when a long bone is unavailable.

• 한국어류학회지
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• 제36권1호
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• pp.40-47
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• 2024

본 연구에서는 드렁허리 Monopterus albus 후각기관의 형태 및 조직학적 특성을 실체, 광학 및 주사전자현미경하 관찰을 통하여 조사하였다. 후각기관의 외부 형태는 피부와 평행한 폐쇄형 전비공과 후비공을 나타냈다. 내부 구조는 파이프형 비강과 뒤쪽의 위아래의 두 개 비낭으로 구성되었다. 비강의 내벽은 위중층상피층의 감각상피와 중층편평상피층의 비감각상피, 미확인기관으로 이루어졌다. 감각상피는 후감각뉴런, 지지세포, 기저세포, 림프구로 구성되며, 비감각상피는 중층상피세포와 산성의 점액다당류를 보유하는 점액세포를 보유했다. 특히, 감각상피층 내 모세혈관과 풍부한 진피에서의 혈관화는 논, 농수로, 연못, 늪과 같은 진흙 하상의 정체된 수환경에 적응 된 후각기관 내 부가적인 호흡기능의 증거이며 이는 이전에 원구류 이상의 어류 분류군에서 확인되지 않은 새로운 결과로 확인된다.