• Journal of the Korean Wood Science and Technology
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• v.30 no.4
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• pp.8-16
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• 2002

This study was performed to produce the high reactive lignin zero substrates from autohydrolyzed wood resources. In chemical compositions of used raw-materials, there were significant differences between two species, Japanese larch (Larix leptolepis) and oak (Quercus mongolica) woods. Japanese larch contained 25 to 3.5 times higher amounts of extractives than oak wood, which is mainly derived from high content of arabinogalactan in Japanese larch wood. Oak wood has 5% lower lignin content and 3% higher holocellulose and pentosans than larch wood. Concerned to changes in wood components during autohydrolysis pretreatment at 22 kg/cm² steaming pressure for 5~60 min, glucose content was constant during pretreatment, while hemicellulose and lignin were abruptly changed. Hemicellulose fraction was decreased significantly and lignin contents increased because of its condensation reaction with hemicellulose degradation products. The pH of hydrolyzates during pretreatment was decreased, reached upto pH 3 and since then leveled off. In the case of oak wood, same tendency was observed as in Japanese larch. Autohydrolysis followed by sodium chlorite and sulfite or bisulfite pretreatment was very effective in delignification of the substrates. In particular, two-stage delignification of autohydrolyzed woods with alkali and O₂-alkali resulted in very low lignin content substrates, such as 0~0.2% lignin substrate.

• Journal of the Korean Chemical Society
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• v.29 no.2
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• pp.172-177
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• 1985

The semiconducting property of Sb-doped tin oxide thin film electrode was investigated and the electrocatalytic effect of this electrode for $SO_2$ (or sulfite, bisulfite ions) oxidation reaction was studied under various conditions. The anodic oxidation of $SO_2$ at tin oxide thin film electrode commenced at lower potential with increasing pH, and good electrocatalytic effect was shown of $SO_3^=$ oxidation in basic solution. In the acidic solutions the electrocatalytic effect of platinum-or palladium-incorporated tin oxide electrode was found to be due to the sites of Pt or Pd exposed on the electrode surface. The electrocatalytic effect of tin oxide electrode was distinctive from that of Pt-or Pd-containing electrodes.

• The Korea Journal of Herbology
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• v.22 no.4
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• pp.45-50
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• 2007

Objectives : The purpose of this study is to determine dose of Gypsum and Natrii sulfas in Shanhanlun and Geumgyeyoryak Methods : We measured the egg size Gypsum, and Natrii sulfas 1 Seong(升), and Results : 1. The weight of GYPSUM, egg is about $48{\pm}4g$, it will end up with 104g, when applying egg gravity of 1.055 and Gypsumls weight of 2.3. 2. One Ryang(兩) is equal to 6.5g in Sanghanlun and Gumgyeyoryak, and therefore the weight of ones gypsum is equal to 1 Keun(斤). 3. Compare to a dose of Gypsum is equal to one egg in Mokbanggitang. 4. Maximum dosage of Gypsum is equal to half an egg, 8Ryang(兩) of Sanghanlun, which is 52g. 5. Daily dosage of One Keun(斤) of Natrii sulfas is 8 Ryang(兩), of sanghanlun, which is 52g, Maximum dosage of Sanghanlun is 4 Ryang(兩), which is 26g. Conclusion : As stated above we may acknowledge that a dosage of Gypsum and Narii sulfas in either Sanghanlun and Keumgyeyoryak is applicable range in practical clinic.

• Genomics & Informatics
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• v.10 no.1
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• pp.9-15
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• 2012

Horizontal gene transfer (HGT) is the movement of genetic material between kingdoms and is considered to play a positive role in adaptation. $Cryptosporidium$ $parvum$ is a parasitic protozoan that causes an infectious disease. Its genome sequencing reported 14 bacteria-like proteins in the nuclear genome. Among them, cgd2_1810, which has been annotated as CysQ, a sulfite synthesis pathway protein, is listed as one of the candidates of genes horizontally transferred from bacterial origin. In this report, we examined this issue using phylogenetic analysis. Our BLAST search showed that $C.$ $parvum$ CysQ protein had the highest similarity with that of proteobacteria. Analysis with NCBI's Conserved Domain Tree showed phylogenetic incongruence, in that $C.$ $parvum$ CysQ protein was located within a branch of proteobacteria in the cd01638 domain, a bacterial member of the inositol monophosphatase family. According to Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway, the sulfate assimilation pathway, where CysQ plays an important role, is well conserved in most eukaryotes as well as prokaryotes. However, the Apicomplexa, including $C.$ $parvum$, largely lack orthologous genes of the pathway, suggesting its loss in those protozoan lineages. Therefore, we conclude that $C.$ $parvum$ regained cysQ from proteobacteria by HGT, although its functional role is elusive.

• Journal of Microbiology and Biotechnology
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• v.28 no.2
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• pp.314-322
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• 2018

Bacillus subtilis 8 is highly efficient at degrading feather keratin. We observed integrated feather degradation over the course of 48 h in basic culture medium while studying the entire process with scanning electron microscopy. Large amounts of ammonia, sulfite, and $\text\tiny{L}$-cysteic acid were detected in the fermented liquid. In addition, four enzymes (gamma-glutamyltranspeptidase, peptidase T, serine protease, and cystathionine gamma-synthase) were identified that play an important role in this degradation pathway, all of which were verified with molecular cloning and prokaryotic expression. To the best of our knowledge, this report is the first to demonstrate that cystathionine gamma-synthase secreted by B. subtilis 8 is involved in the decomposition of feather keratin. This study provides new data characterizing the molecular mechanism of feather degradation by bacteria, as well as potential guidance for future industrial utilization of waste keratin.

• KSBB Journal
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• v.21 no.6 s.101
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• pp.489-492
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• 2006

For molecular breeding purpose, genetic transformation of Brassica napus cultivars has been extensively performed using Agrobacterium method. B. napus cv. napus, one of major oil crops, can be transformed via Agrobacterium-based method. We demonstrated that Agrobacterium-mediated transformation via vacuum infiltration slightly worked for the seedlings of B. napus cv. napus according to fluorometric GUS enzyme analysis. In contrast, transformation efficiency was highly enhanced when the seedlings, prior to agroinfiltration, were treated with sodium hydrosulfite solution as a chemical wounding agent. GUS gene expression in transformed seedlings that was confirmed by RT-PCR suggests their usefulness for the development of transient expression system.

• Fashion & Textile Research Journal
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• v.14 no.3
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• pp.472-477
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• 2012

The conditions of wet spinning were considered in order to prepare the fine denier of acrylic fiber. Polyacrylonitrile copolymer was synthesized by the copolymerization of acrylonitrile (AN) and methyl acrylate (MA) initiated by an aqueous sulfite-chlorate redox system. Acrylic fiber was manufactured through wet-spinning in a dimethyl formamide (DMF) system. The conditions of wet-spinning were investigated by i-value, spinning speed, diameter of spinneret, draw ratio, water content of spinning dope and morphology of protofiber. The physical properties of fibers were investigated by Instron. In this experiment, the minimum i-value decreased with the decreasing spinneret diameter, an increased spinning speed, and an increased coagulation bath (CBC) concentration. The maximum draw ratio increased with an increased CBC. The optimum CBC and water content of the spinning dope were 60%-65% and 3.5%, respectively. The tenacity at the breaking point increased with a decreased fineness of fiber. The elongation at breaking point was almost the same value as a function of the fineness of fiber.

• Bulletin of the Korean Chemical Society
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• v.35 no.7
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• pp.2109-2113
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• 2014

Solvent extraction of Fe(III) from chloride solution by using a mixture of D2EHPA (Di-(2-ethylhexyl)-phosphoric acid) and TBP (Tri-butyl phosphate) and the reductive stripping of Fe(III) from the loaded organic were investigated. Quantitative extraction of Fe(III) from the solution (Fe concentration = 90 g/L) was accomplished in two cross-current extraction stages by using the mixture of D2EHPA and TBP. In order to facilitate the stripping efficiency, a reductive stripping method was employed by using $H_2SO_3$ or $Na_2SO_3$ as a reducing agent. The addition of $H_2SO_4$ into reducing agents led to improvement in the stripping efficiency while high concentration acid would suppress it. Both of the mixtures of $H_2SO_4+H_2SO_3$ and $H_2SO_4+Na_2SO_3$ showed good efficiency for the stripping of Fe(III), while the latter was recommended as the stripping solution based on the economics and experimental condition.

• Korean Journal of Plant Resources
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• v.10 no.3
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• pp.221-226
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• 1997

Improvement of seed protein quality might be an essential issus in soybean and would give more profit directly to both farmers and users. This study was carried out to investigate the effects of reduced-S form(s) on seed storage protein components in soybean during seed filling stages. The reduced-S forms during seed fill were sodium thiosulfate, sodium sulfite, sodium sulfide, thioaceteat, $\beta$-mercaptoethanol, thiourea, thiamine-HCI, L-cysteine, L-cystine, and L-methionine. Seed storage protein concentration did not appear to be affected by any reduced-S forms. However, glycinin and $\beta$-conglycinin concentration seemed to be changed greatly by L-methionine. This resulted in the increase in the 11S/7S ratio(3.58). Among the $\beta$-conglycinin, $\beta$-subunit was not accumulated at all. $\alpha$-subunit concentration appeared to be decreased and $\alpha'$-subunit concentration was not altered in comparison with sulfate control. Also, $\beta$-conglycine concentration, especially $\beta$-subunit concentration, tended to be decreased with L-cystine treatment, resulting in an increase in the 11S/7S ratio(1.83). The glycinin concentration tended to be increased at the expense of the decrease in the $\beta$-conglycinin concentration. Therefore, it is suggested that enhancing soybean protein quality would be achieved by improving metabolic pathways of S assimilation in soybean plants during seed filling period under sulfate-sufficient condition.

• Journal of The Korean Society of Inherited Metabolic disease
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• v.14 no.1
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• pp.10-18
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• 2014

Recent advances in the diagnosis and treatment of inborn errors of metabolism have improved substantially the prognosis of many of these diseases, if diagnosed early enough before irreversible damage occurs. This makes it essential that the practicing pediatrician, especially neonatologists be familliar with the clinical presentations and systematic approaches of these disorders. Characteristic clinical presentations, methods of systematic approach and typing of various disorders is discussed in this review. The signs of neurological dysfunctions of many IEMs manifesting in the neonatal period is very nonspecific, such as poor feeding, poor sucking, apnea or tachypnea, vomiting, hypertonia, hypotonia, seizure, letharginess, consciousness change and coma. Many other non-metabolic severe disorders of neonatal period such as neonatal sepsis and intracerebral hemorrhage share these nonspecific symptoms. Hyperammonemia, metabolic acidosis, ketosis and hyperlatic acidemia are observed in many of these conditions but there are exceptions in which conditions all basal laboratory tests are normal, such as NKH, sulfite oxidase deficiency and peroxisomal disorders. According to the results of basal laboratory tests, IEMs in the neonatal period can be categorized in to 6 types. Grouping of IEMs into 6 types will make confirmatory tests and early emergency treatment more efficient.