• Journal of Microbiology and Biotechnology
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• v.18 no.5
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• pp.908-912
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• 2008

To achieve a higher succinic acid productivity and evaluate the industrial applicability, this study used Mannheimia succiniciproducens LPK7 (knock-out: ldhA, pflB, pta-ackA), which was recently designed to enhance the productivity of succinic acid and reduce by-product secretion. Anaerobic continuous fermentation of Mannheimia succiniciproducens LPK7 was carried out at different glucose feed concentrations and dilution rates. After extensive fermentation experiments, a succinic acid yield and productivity of 0.38 mol/mol and 1.77 g/l/h, respectively, were achieved with a glucose feed concentration of 18.0 g/l and $0.2\;h^{-1}$ dilution rate. A similar amount of succinic acid production was also produced in batch culture experiments. Therefore, these optimal conditions can be industrially applied for the continuous production of succinic acid. To examine the quantitative balance of the metabolism, a flux distribution analysis was also performed using the metabolic network model of glycolysis and the pentose phosphate pathway.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.18 no.3
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• pp.227-234
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• 1985

The present study was carried out for making clear the organic acids composition of some shellfishes and their boiled-dried products. Three species of shellfish such as top shell, hard clam and abalone were chosen as the experimental samples and analysed for their nonvolatile organic acids composition by gas liquid chromatography (GLC). Eight kinds of nonvolatile organic acid were identified in raw and boiled-dried top shell, major ones were succinic, malic and pyroglutamic acid. Out of nine kinds of nonvolatile acid was determined in raw and boiled-dried of hard clam, abundant ones were succinic, lactic and malic acid, And seven kinds of nonvolatile organic acid were detected in boiled-dried abalone while eight kinds in raw sample, abundant ones were succinic, lactic and pyroglutamic acid, respectively. In all samples, the total amount of nonvolatile organic acid was the most abundant in hard clam while the least in abalone, and the content of succinic acid was appeared as the most abundant one. In dried samples, the total amount of the nonvolatile organic acid was determined to be about half-decrease after the boiled-dried treatment of the raw samples, of which abalone showed the highest decreasing을 rate whereas hard clam was the lowest. In general, the decreasing rate of each acid was the higher in fumaric, malic and citric acid than in maleic, oxalic and succinic acid.

• Microbiology and Biotechnology Letters
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• v.26 no.6
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• pp.545-550
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• 1998

Succinic acid, valuable $C_4$-dicarboxylic acid as a renewable alternative feedstock, is currently produced commercially by the petrochemical process, but extensive efforts have been devoted to establish the biological process for mass production of succinic acid. In this study, the bioconversion of fumaric acid to succinic acid was investigated. We isolated an Enterococcus sp. RKY1 KCTC 8890P, facultative bacterium, capable of the bioconversion of fumaric acid to soccinic acid very rapidly and efficiently. At batch fermentation, the amount of succinic acid production increased with increase in initial fumaric acid from 40 to 100 g/L. With fumaric acid of 70 g/L, the average specific and volumetric production rate, molar yield were reached up to 0.64 g/g.h, 4.87 g/g.h, and 96.5%, respectively. Maximum concentration of succinic acid of 88.9 g/L was achieved with molar yield of 89% with fumaric acid of 100 g/L in less than 20 hours.

• Textile Coloration and Finishing
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• v.21 no.3
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• pp.1-9
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• 2009

Polycarboxylics acids are used as crosslinking agents for cotton cellulose to produce durable finished press cotton fabric. It has been observed that the strength of the cotton fabric treated with polycarboxylic acids showed significant reduction as a result of the crosslinking process. The effect of acid-catalyzed depolymerization on the tear strength of cotton fabric is investigated by evaluating the cotton fabric treated by succinic acid, which does not crosslink cotton cellulose and form little ester on the cotton fabric. We find that the tear strength of cotton fabric treated with succinic acid decreases at elevated temperature due to acid-catalyzed depolymerization of cellulose. The magnitude of fabric strength reduction increases as the acid concentration increases. At a constant acid concentration, it increases as the curing temperature and time increases. It decreases as the pH of the acid solution increases. We also find that the dissociation constant of an acid also has a significant effect on the fabric strength reduction. The magnitude of fabric tear strength reduction increases as the acid dissociation constant decreases.

• KSBB Journal
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• v.20 no.3
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• pp.142-148
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• 2005

Succinic acid is an important material in industries producing biodegradable polymers, food and pharmaceutical products, and green solvents. Furthermore, succinate fermentation is a novel process due to the fixation of $CO_2$ into succinate during fermentation. However, the impurities in fermentation broth make the separation process of succinic acid be difficult. Reactive extraction has been proposed to be an effective primary separation step of succinic acid from dilute fermentation broth. This article presents the principles of reactive extraction along with the characteristics of tertiary amino extractants. A brief overview on the current research on reactive extraction of succinic acid is presented. Finally, for the succinic acid separation, reactive extraction as a primary step is suggested in the whole downstream process for succinic acid from fermentation broth.

• Journal of Microbiology and Biotechnology
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• v.18 no.11
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• pp.1792-1796
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• 2008

Succinic acid-producing Anaerobinspirillum succiniciproducens was anaerobically grown on galactose, galactose/glucose, or galactose/lactose in order to study its galactose fermentation. Unlike a previous report, A. succiniciproducens was found to efficiently metabolize galactose as the sole carbon source at a rate of 2.4 g/g-DCW/h and produced succinic acid with as high a yield of 87% as with using glucose. When glucose and galactose were present, A. succiniciproducens metabolized both sugars simultaneously. Furthermore, when lactose and galactose coexisted, lactose did not inhibit the galactose fermentation of A. succiniciproducens. Therefore, co-utilization of galactose and other sugars can improve the productivity and economy of bio-based succinic acid processes.

• Journal of Microbiology and Biotechnology
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• v.6 no.1
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• pp.43-49
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• 1996

Modulation of the catabolic PEP-pathway of Anaerobiospirillum succiniciproducens was tried using some enzymatic inhibitors such as gases and chemicals in order to enhance succinic acid production. 10$\%$ CO increased the succinic acid/acetic acid (S/A) ratio but inhibited growth as well as production of succinic and acetic acid. Hydrogen gas also increased the S/A ratio and inhibited the synthesis of pyruvate: ferredoxin oxidoreductase when used in mixture with $CO_2$, Catabolic repression by acetic, lactic and formic acid was not recognized and other modulators such as glyoxylate, pyruvate derivatives, arsenic salt, phosphate and sulfate were shown not to be effective. Magesium carbonate was shown effective for repressing acetate production. Palmitic acid, myristic acid and phenylalanine did not affect acetate production but carprylic acid completely inhibited growth.

• 한국생물공학회:학술대회논문집
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• 2000.11a
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• pp.338-341
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• 2000

An internal membrane bioreactor system was employed for continuous succinic ac id production from glucose in order to prove its performance and practicality. Succinic acid-producing Anaerobiospirillum succiniciproducens required more $CO_2$ for the proper growth and succinic acid production in cell recycled continuous culture than in batch culture. The maximum productivity obtained in cell recycled continuous culture was about 3.3 g/L-h which was ca. 3.3 times higher than that obtained in batch culture.

• Journal of Microbiology and Biotechnology
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• v.18 no.7
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• pp.1252-1256
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• 2008

Cell recycled culture of succinic acid-producing Anaerobiospirillum succiniciproducens was anaerobically carried out using an internal membrane filter module in order to examine the physiological response of A. succiniciproducens to a high-cell-density environment. The optimal growth of A. succiniciproducens and its enhanced succinic acid productivity were observed under $CO_2$-rich conditions, established by adding $NaHCO_3$ and $Na_2CO_3$, in the cell recycled system. A. succiniciproducens grew up to 6.50 g-DCW/l, the highest cell concentration obtained so far, in cell recycled cultures. The cells did not change their morphology, which is known to be easily changed in unfavorable or stress environments. The maximum productivity of succinic acid was about 3.3 g/l/h, which is 3.3 times higher than those obtained in batch cultures. These results can serve as a guide for designing highly efficient cell recycled systems for succinic acid at a commercial level.

• Korean Chemical Engineering Research
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• v.50 no.6
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• pp.1021-1026
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• 2012

The transient mass transfer in a single droplet system consisting of 1-octanol (continuous phase)/aqueous succinic acid solution (dispersed phase) was investigated in the presence of chemical reaction, which is acid/anion exchange reaction of succinic acid and tri-n-octylamine (TOA). This succinic acid extraction by TOA can be considered to occur at the interface between organic and aqueous phase, that is, heterogeneous reaction system. The basic properties of the system such as viscosity, density, distribution coefficient, terminal velocity of droplet, and diffusion coefficient were measured experimentally or calculated theoretically, and used for theoretical calculation of characteristic parameters of mass transfer later. The effects of succinic acid concentration on the terminal velocity was negligible in the existence of TOA, although the terminal velocity increases with succinic acid concentration in the absence of TOA. On the contrary, the terminal velocity decreases with TOA concentration. While droplets falls through organic phase, the trajectory of droplets is observed to oscillate around its vertical path. A mass trnasfer cell was prepared to monitor the mass transfer behavior in a single droplet and used to measure the mean concentration of succinic acid inside droplet. The results are expressed with dimensionless parameters. Under 50 g/L succinic acid condition, the system with 0.1 mol/kg TOA showed that the molar flux decreases in proportion to the decrease of concentration gradient, while in the case of 0.5 mol/kg TOA Sh increases rapidly with time indicating the molar flux of succinic acid decreases relatively slowly compared to the decrease in concentration gradient.