• Proceedings of the KIEE Conference
• /
• 2004.11d
• /
• pp.93-96
• /
• 2004

In this paper, we analyzed the current limiting characteristics according to increase of source voltage in the flux-lock type high-Tc superconducting fault current limiter (SFCL). The flux-lock type SFCL consisted of two coils, which were wound in parallel each other through an iron core, and high-Tc superconducting (HTSC) element connected with coil 2 in series. The flux-lock type SFCL has the characteristics better in comparison with the resistive type SFCL because the fault current in the flux-lock type SFCL can be divided into two coils by the inductance ratio of coil 1 and coil 2. The fault current limiting operation of the flux-lock type SFCL can be different due to winding direction of the two coils. The winding method where the decrease of linkage flux between two coils in the accident happens is called the subtractive polarity winding and the winding method in case of the increase of linkage flux is called the additive polarity winding. The fault current limiting experiments according to the source voltage were performed for these two winding methods. Through the comparison and the analysis of the experimental data, we confirmed that the quench time was shorter, irrespective of the winding direction as the source voltage increased and that the fault current and the HTSC's resistance increased as the amplitude of the source voltage increased. The additive polarity winding made the fast quench time and the lower resistance of HTSC element in comparison with the subtractive polarity winding. The fault current of the subtractive polarity winding was larger than that of the additive polarity winding. In conclusion, we found that the additive polarity winding reduced the burden of SFCL because the quench time was shorter and the fault current was smaller than those of the subtractive polarity winding.

• Radiation Oncology Journal
• /
• v.23 no.1
• /
• pp.43-50
• /
• 2005

Purpose : A number of genes and their products are Induced early or late following exposure of cells to ionizing radiation. These radiation-Induced genes have various effects on irradiated cells and tissues. Suppression subtractive hybridization (SSH) based on PCR was used to Identify the differentially expressed genes by radiation in cervix carcinoma cells. Materials and Methods : Total RNA and poly $(A)^+$ mRNA were Isolated from Irradiated and non-irradiated HeLa cells. Forward- and reverse-subtracted cDNA libraries were constructed using SSH. Eighty-eight clones of each were used to randomly select differentially expressed genes using reverse Northern blotting (dot blot analysis). Northern blotting was used to verify the screened genes. Results : Of the 17t clones, 10 genes in the forward-subtracted library and 9 genes In the reverse-subtracted library were identified as differentially expressed radiation-induced genes by PCR-select differential screening. Three clones from the forward-subtracted library were confirmed by Northern blotting, and showed increased expression in a dose-dependent manner, including a telomerase catalytic subunit and sodium channel-like protein gene, and an ESTs (expressed sequence tags) gene. Conclusion : We Identified differentially expressed radiation-induced genes with low-abundance genes with SSH, but further characterization of theses genes are necessary to clarify the biological functions of them.

• Journal of Technologic Dentistry
• /
• v.45 no.2
• /
• pp.31-38
• /
• 2023

Purpose: This in vitro study aimed to compare the trueness of 3-unit fixed dental provisional prostheses (FDPs) fabricated by three different additive manufacturing and subtractive manufacturing procedures. Methods: A reference model with a maxillary left second premolar and the second molar prepped and the first molar missing was scanned for the fabrication of 3-unit FDPs. An anatomically shaped 3-unit FDP was designed on computer-aided design software. 10 FDPs were fabricated by subtractive (MI group) and additive manufacturing (stereolithography: SL group, digital light processing: DL group, liquid crystal displays: LC group) methods, respectively (N=40). All FDPs were scanned and exported to the standard triangulated language file. A three-dimensional analysis program measured the discrepancy of the internal, margin, and pontic base area. As for the comparison among manufacturing procedures, the Kruskal-Wallis test and the Mann-Whitney test with Bonferroni correction were evaluated statistically. Results: Regarding the internal area, the root mean square (RMS) value of the 3-unit FDPs was the lowest in the MI group (31.79±6.39 ㎛) and the highest in the SL group (69.34±29.88 ㎛; p=0.001). In the marginal area, those of the 3-unit FDPs were the lowest in the LC group (25.39±4.36 ㎛) and the highest in the SL group (48.94±18.98 ㎛; p=0.001). In the pontic base area, those of the 3-unit FDPs were the lowest in the LC group (8.72±2.74 ㎛) and the highest in the DL group (20.75±2.03 ㎛; p=0.001). Conclusion: A statistically significant difference was observed in the RMS mean values of all the groups. However, in comparison to the subtractive manufacturing method, all measurement areas of 3-unit FDPs fabricated by three different additive manufacturing methods are within a clinically acceptable range.

• Proceedings of the Korea Environmental Mutagen Society Conference
• /
• 2003.10a
• /
• pp.130-130
• /
• 2003

• 대한치주과학회:학술대회논문집
• /
• 2007.11a
• /
• pp.52-52
• /
• 2007

• 한국초전도학회:학술대회논문집
• /
• v.14
• /
• pp.58-58
• /
• 2004

• IMMUNE NETWORK
• /
• v.4 no.4
• /
• pp.237-243
• /
• 2004

Background: The normal functions of the cell cycle inhibitor p16INK4a are frequently inactivated in many human cancers. Over 80% of hepatocellular carcinoma (HCC) cases lack a functional p16/Rb pathway. p16/Rb pathway, as well as p53 pathway, is considered as one of key components of tumor suppression. Methods: To study the roles of p16INK4a in HCC, a stable cell line expressing exogenous p16 was generated from SNU-449 hepatocellular carcinoma cells lacking endogenous p16, and suppression subtractive hybridization (SSH) was performed in parallel with the control cells. Results: 1) SSH identifies fibronectin (FN1), crystallin ${\alpha}B$ (CRYAB), Rac1, WASP, RhoGEF, and CCT3 as differentially-expressed genes. 2) Among the selected genes, the up-regulation of FN1 and CRYAB was confirmed by Northern blot, RT-PCR and by proteomic methods. Conclusion: These genes are likely to be associated with the induction of stress fiber and stabilization of cytoskeleton. Further studies are required to clarify the possible role of p16 in the signal transduction pathway.

• International Journal of Industrial Entomology and Biomaterials
• /
• v.33 no.2
• /
• pp.102-107
• /
• 2016

Stress may be defined as any modification of environmental parameters that leads to a response by biological organisms. Stresses that affect biolpgical structures may be nonthermal, such as ultraviolet radiation, pH, or salinity, or thermal. Temperture is one of the major stresses that all living organism face. The major effects of cold(low emperature) are decrease of membrane fluidity and the stabilization of secondary structures of RNA and DNA in the cells, which may effect the efficiency of translation, transcription, and DNA replication. In this study, we focus on discovering the genes that are expressed by the cold(low temperature) stress in the silkworm. In cold(low temperature) stress test, we found 100% survive from cold stress at $0^{\circ}C$ up to 12h and $-5^{\circ}C$ up to 2h, and then, survive rate was rapidly decrease in silkworm. Thereafter two whole genes have selected by SSH(Suppression subtractive hybridization). (GenBank accession : GQ149511, GQ338156)

• Smart Structures and Systems
• /
• v.16 no.6
• /
• pp.1107-1132
• /
• 2015

A damage detection algorithm based on neuro fuzzy hybrid system is presented in this study for location and severity predictions of cracks in beam-like structures. A combination of eigenfrequencies and rotation deviation curves are utilized as input to the soft computing technique. Both single and multiple damage cases are considered. Theoretical expressions leading to modal properties of damaged beam elements are provided. The beam formulation is based on Euler-Bernoulli theory. The cracked section of beam is simulated employing discrete spring model whose compliance is computed from stress intensity factors of fracture mechanics. A hybrid neuro fuzzy technique is utilized to solve the inverse problem of crack identification. Two different neuro fuzzy systems including grid partitioning (GP) and subtractive clustering (SC) are investigated for the highlighted problem. Several error metrics are utilized for evaluating the accuracy of the hybrid algorithms. The study is the first in terms of 1) using the two models of neuro fuzzy systems in crack detection and 2) considering multiple damages in beam elements employing the fused neuro fuzzy procedures. At the end of the study, the developed hybrid models are tested by utilizing the noise-contaminated data. Considering the robustness of the models, they can be employed as damage identification algorithms in health monitoring of beam-like structures.

• Journal of Korean society of Dental Hygiene
• /
• v.19 no.6
• /
• pp.1089-1097
• /
• 2019

Objectives: The purpose of this study is to assess the accuracy of provisional restorative resins fabricated using dental three-dimensional (3D) printers. Methods: Provisional restorative resins were fabricated using the first molar of the right mandibular. Three groups comprising a total of 24 samples of such resins were fabricated. The prepared abutment was scanned initially and then designed using a computer-aided design (CAD) software. The conventional subtractive manufacturing system was employed to fabricate the first group of resins, while the second and third groups were fabricated using a digital light processing (DLP) 3D printer and a stereolithography (SLA) 3D printer, respectively. The internal surfaces of the resins were scanned and 3D measurements of the resins were taken to confirm their accuracy. Results: The root-mean-square deviation (RMS±SD) of the accuracy of the resins fabricated using the conventional subtractive manufacturing system, DLP 3D printer, and SLA 3D printer were 68.83±2.22 ㎛, 74.63±6.23 ㎛, and 61.74±4.09 ㎛, respectively. A one-way analysis of variance (ANOVA) test showed significant differences between the three groups (p < 0.05). Conclusions: Provisional restorative resins fabricated using DLP and SLA 3D printers demonstrated clinically-acceptable results.