• Title/Summary/Keyword: subpopulation

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The effect of Asparagi Tuber on Anti-cancer and Immunocytes (천문동(天門冬)이 항암(抗癌) 및 면역세포(免疫細胞)에 미치는 영향(影響))

  • Jeong, Hyun Woo;Cho, Young-Lim
    • Herbal Formula Science
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    • v.5 no.1
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    • pp.169-178
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    • 1997
  • To investigate effect of water extract of Asparagi Tuber(天門冬) on human cancer cell-lines and immunocytes, this research estimated proliferation of A431 cell line, KHOS-NP cell line, mouse thymocytes and mouse splenocytes, Nitric Oxide(NO) from macrophage, apoptosis and subpopulation of the mouse thymocytes. The result were obtained as follows ; 1. Asparagi Tuber inhibited the proliferation of A431 cell line. 2. Asparagi Tuber inhibited the proliferation of KHOS-NP cell line. 3. Asparagi Tuber accelerated the proliferation of mouse thymocytes. 4. Asparagi Tuber inhibited the proliferation of mouse splenocytes. 5. Asparagi Tuber $100{\mu}g/m{\ell}$ inhibited the production of NO from macrophages in vitro, being compared NPS+IFN treated group. 6. Asparagi Tuber inhibited the production of NO from macrophages in vivo, being compared LPS+IFN treated group. 7. Asparagi Tuber accelerated the induction of apoptosis of the mouse thymocytes. 8. In subpopulation Asparagi Tuber increased $T_H$ of the mouse thymocytes, but decreased $T_C/T_S$ of the mouse thymocytes.

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Correlation analyses of CpG island methylation of cluster of differentiation 4 protein with gene expression and T lymphocyte subpopulation traits

  • Zhao, Xueyan;Wang, Yanping;Guo, Jianfeng;Wang, Jiying
    • Asian-Australasian Journal of Animal Sciences
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    • v.31 no.8
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    • pp.1141-1149
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    • 2018
  • Objective: Cluster of differentiation 4 protein (CD4) gene is an important immune related gene which plays a significant role in T cell development and host resistance during viral infection. Methods: In order to unravel the relationship of CpG island methylation level of CD4 gene with its gene expression and T lymphocyte subpopulation traits, we used one typical Chinese indigenous breed (Dapulian, DP) and one commercial breed (Landrace), then predicted the CpG island of CD4 gene, determined the methylation status of CpG sites by bisulfite sequencing polymerase chain reaction (BSP), and carried out the correlation analyses of methylation frequencies of CpG sites with mRNA expression and T lymphocyte subpopulation traits. Results: There was one CpG island predicted in the upstream -2 kb region and exon one of porcine CD4 gene, which located 333 bp upstream from the start site of gene and contained nine CpG sites. The correlation analysis results indicated that the methylation frequency of CpG_2 significantly correlated with CD4 mRNA expression in the DP and Landrace combined population, though it did not reach significance level in DP and Landrace separately. Additionally, 15 potential binding transcription factors (TFs) were predicted within the CpG island, and one of them (Jumonji) contained CpG_2 site, suggesting that it may influence the CD4 gene expression through the potential binding TFs. We also found methylation frequency of CpG_2 negatively correlated with T lymphocyte subpopulation traits CD4+CD8-CD3-, CD4-CD8+CD3- and CD4+/CD8+, and positively correlated with CD4-CD8+CD3+ and CD4+CD8+CD3+ (for all correlation, p<0.01) in DP and Landrace combined population. Thus, the CpG_2 was a critical methylation site for porcine CD4 gene expression and T lymphocyte subpopulation traits. Conclusion: We speculated that increased methylation frequency of CpG_2 may lead to the decreased expression of CD4, which may have some kind of influence on T lymphocyte subpopulation traits and the immunity of DP population.

In vitro Change of IgG Production by Administration of Cortisol Lipopolysaccharide Treated Lymphocyte of Cat (In vitro에서 cortisol투여에 의한 LPS 전처치 고양이 림프구의 IgG생산변화)

  • 나기정
    • Journal of Veterinary Clinics
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    • v.15 no.1
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    • pp.8-13
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    • 1998
  • Change of IgG production of feline mononuclear cell(MNC) was evalual vitro. MNC was treated with lipopolysaccharide(LPS) before cortisol administration. tisol induced change of B cell subpopulation with surface IgG and reduced IgG prods against virus. However, before treatment o$\ulcorner$ MNC with LPS induced increasement of subpopulation with surface IgG and IgG production against virus. These results impel: diminution of IgG production by cortisol is well again by LPS treatment.

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Nodal tumor response according to the count of peripheral blood lymphocyte subpopulations during preoperative chemoradiotherapy in locally advanced rectal cancer

  • Heo, Jaesung;Oh, Young-Taek;Noh, O Kyu;Chun, Mison;Park, Jun-Eun;Cho, Sung-Ran
    • Radiation Oncology Journal
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    • v.34 no.4
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    • pp.305-312
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    • 2016
  • Purpose: The objective of this prospective study was to evaluate the relationship between the circulating lymphocyte subpopulation counts during preoperative chemoradiotherapy (CRT) and tumor response in locally advanced rectal cancer. Materials and Methods: From August 2015 to June 2016, 10 patients treated with preoperative CRT followed by surgery were enrolled. Patients received conventional fractionated radiotherapy (50.4 Gy) with fluorouracil-based chemotherapy. Surgical resection was performed at 4 to 8 weeks after the completion of preoperative CRT. The absolute blood lymphocyte subpopulation was obtained prior to and after 4 weeks of CRT. We analyzed the association between a tumor response and change in the lymphocyte subpopulation during CRT. Results: Among 10 patients, 2 (20%) had evidence of pathologic complete response. In 8 patients with clinically node positive, 4 (50%) had nodal tumor response. All lymphocyte subpopulation counts at 4 weeks after CRT were significantly lower than those observed during pretreatment (p < 0.01). A high decrease in natural killer (NK) cell, count during CRT (baseline cell count - cell count at 4 weeks) was associated with node down staging (p = 0.034). Conclusion: Our results suggest that the change of lymphocyte subset to preoperative CRT may be a predictive factor for tumor response in rectal cancer.

Differences in Cancer Incidence among Predominantly Muslim and Buddhist Subpopulations in Songkhla

  • Sriplung, Hutcha;Bilheem, Surichai;Kuntipundee, Tirada;Geater, Sarayut Lucian
    • Asian Pacific Journal of Cancer Prevention
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    • v.15 no.22
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    • pp.9979-9983
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    • 2014
  • Background: The population of Songkhla, a province in Southern Thailand, can be divided into a predominantly Muslim subpopulation (PMSP, approximately 70% Muslim) and a predominantly Buddhist subpopulation (PBSP, around 14% Muslim). Objectives: This study was conducted to 1) describe the incidence of various cancers in both PMSP and PBSP, and 2) compare the incidence of various cancers between the two subpopulations. Materials and Methods: Cancer cases diagnosed between 1990 and 2010 were drawn from the database of Songkhla Cancer Registry. Population denominators were estimated from the 3 population censuses surveyed by the National Statistical Office of Thailand in 1990, 2000, and 2010. Results: The age-standardized incidence rates (ASR) of the 5 commonest male cancers among both subpopulations were calculated. In females, a lower incidence of cancers of the cervix and breast in PMSP compared to PBSP, with odds ratios of 0.54 (95% CI: 0.45-0.64) and 0.51 (95% CI: 0.43-0.60) respectively, was observed. In males, the incidence of cancers of the lung, liver, colon-rectum, and some other cancers were significantly different between the two populations in the past, but only prostate cancer showed a lower incidence among males in PMSP in recent years. Independent of sex and year of diagnosis, the incidence of lung, liver, NHL, and colorectal cancers was lower in MPSP compared to BPSP, with odds ratios of 0.75 (95% CI: 0.65-0.85), 0.74 (95% CI: 0.62-0.88), 0.74 (95% CI: 0.60-0.91), and 0.67 (95% CI: 0.56-0.78) respectively. Conclusions: The differences in incidence of some cancers and religionrelated culture between the two subpopulations need 2 sets of cancer-control plans and goals to fit the unique population context in deep Southern Thailand. This plan can be used in the 3 southernmost provinces of Thailand where the percentage of Muslims is over 85%.

Subpopulation in periopheral blood leukocyte of Korean native cattle by using monoclonal antibodies specific to bovine leukocyte differentiation antigen (백혈구 표면항원 특이 단크론항체를 이용한 한우의 말초혈액 백혈구 아군에 관한 연구)

  • Moon, Jin-san;Park, Yong-ho;Jung, Suk-chan;Ku, Bok-gyeong;Kang, Byong-kyu
    • Korean Journal of Veterinary Research
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    • v.36 no.2
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    • pp.337-348
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    • 1996
  • The proportion of leukocyte subpopulation in the host is of a great importance in understanding their functions and disease progress. Many methods have been developed to seperate leukocytes and to measure their activities. Characterization of immune cell subpopulations in Korean native cattle was performed using a set of monoclonal antibodies specific which are specific to bovine leukocyte differentiation antigen. Peripheral blood leukocytes from fifty Korean native and ten Holstein cattle were collected and analyzed for the investigation of leukocyte subpopulation by using monoclonal antibodies and flow cytometry. The result indicated that Korean native cattle have significantly higher proportion of leukocyte subpopulations expressing MHC class II molecules and BoCD4 than Holstein cattle.

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Effect of Bambusae Caulis in Liquamen on T-lymphocytes and Peritoneal Macrophages (죽력(竹瀝)이 T-lymphocytes 및 복강(腹腔) Macrophage에 미치는 영향(影響))

  • Jeong, Hyun-Woo;Kang, Sung-Yong;Han, Jong-Hyun;Lee, Won-Suk;Oh, Chan-Ho;Eun, Jae-Soon
    • The Journal of Internal Korean Medicine
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    • v.18 no.2
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    • pp.27-39
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    • 1997
  • The purpose of this research was to investigate effects of Bambusae Caulis in Liquamen(BCL) on T-lymphocytes and peritoneal macrophages in mice. The apoptosis and subpopulation of T-lymphocytes were tested using a flow cytometer. The phagocytic activity of mouse peritoneal macrophage was tested using a luminometer. Nitric oxide production was tested using a Griess reagents. BCL induced T-lymphocytes apoptosis. BCL increased $T_H$ cells population and decreased $T_C$ cells population of T-lymphocyte, but did not affect splenocytes subpopulation. BCL increased nitric oxide production and phagocytic activity of peritoneal macrophage in mice. These results suggest that BCL regulates the immune system in consequence of an increase in helper T cell population and macrophages activation.

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Sensor placement optimization in structural health monitoring using distributed monkey algorithm

  • Yi, Ting-Hua;Li, Hong-Nan;Zhang, Xu-Dong
    • Smart Structures and Systems
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    • v.15 no.1
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    • pp.191-207
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    • 2015
  • Proper placement of sensors plays a key role in construction and implementation of an effective structural health monitoring (SHM) system. This paper proposes a novel methodology called the distributed monkey algorithm (DMA) for the optimum design of SHM system sensor arrays. Different from the existing algorithms, the dual-structure coding method is adopted for the representation of design variables and the single large population is partitioned into subsets and each subpopulation searches the space in different directions separately, leading to quicker convergence and higher searching capability. After the personal areas of all subpopulations have been finished, the initial optimal solutions in every subpopulation are extracted and reordered into a new subpopulation, and the harmony search algorithm (HSA) is incorporated to find the final optimal solution. A computational case of a high-rise building has been implemented to demonstrate the effectiveness of the proposed method. Investigations have clearly suggested that the proposed DMA is simple in concept, few in parameters, easy in implementation, and could generate sensor configurations superior to other conventional algorithms both in terms of generating optimal solutions as well as faster convergence.

Effects of Boheo-tang and Boheo-tang plus Cervi Pantotrichum Cornu : on Immune Response in Postpartum mice (보허탕(補虛湯)과 보허탕가녹용(補虛湯加鹿茸)이 산후 생쥐의 면역반응에 미치는 영향)

  • Lee, Eun-Hee;Kim, Tae-Hee
    • The Journal of Korean Obstetrics and Gynecology
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    • v.22 no.3
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    • pp.25-35
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    • 2009
  • Purpose: The purpose of this study is to investigate the effect of Boheo-tang (B) and Boheo-tang plus cervi pantotrichum cornu (B+CP) on immune response in postpartum C57BL/6N mice. Methods: Normal saline(control), B and B+CP (8${\mu}{\ell}$/g) were administerd p.o. twice a day for 20 days. Subpopulation of T and B lymphocyte were accessed by flow cytometric analysis. Results: Splenic T and B lymphocytes were increased by the treatment of B. Subpopulation of cytotoxic T lymphocytes in the spleen, were significantly increased by both treatment of B and B+CP. Subpopulation of cytotoxic T lymphcytes in the thymus, were significantly increased by both treatment of B and B+CP. IL-4 production was significantly increased by the treatment of B+CP. Conclusion: This study shows that treatment of Boheo-tang and Boheo-tang plus cervi pantotrichum cornu can improve postpartum immune response in C57BL/6N mice.

Analysis of T Lymphocyte Subpopulation Change Defined by Monoclonal Antibodies Immediately after Radiotherapy (단클론항체를 이용한 방사선치료 직후의 T 임파구아형의 변화에 관한 연구)

  • Choi, Gyu-Young;Choi, Wan, Sung
    • Radiation Oncology Journal
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    • v.10 no.2
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    • pp.129-135
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    • 1992
  • We studied the T lymphocyte and its subpopulation percentage change in 40 patients immediately after the radiation therapy. Study population consisted of 12 patients treated at the site of head and neck region,14 patients treated at the site of thoracic region, and 14 patients treated at the site of pelvic region. Twenty two patients received radiotherapy as radical modality, and remaining 18 patients received radiotherapy as postoperative modality. Immediately after radiotherapy, total T lymphocyte (T1) percentage was decreased from $56.4\%$ to $55.2\%$, helper T cell (T4) percentage was decreased from $36.4\%$ to $34.1\%$, but suppressor T lymphocyte (T8) percentage was increased from $23.5\%$ to $25.4\%$. As a result, T4/T8 ratio was decreased from 1.57 to 1.39. This study suggested that immediate change after radiotherapy of the T lymphocyte and its subpopulation percentage was not related to the treatment volume and the degree of helper T cell decrement was not pronounced by the radiation dose increment. Long-term follow-up study En larger scale is needed to determine long term changing pattern in T lymphocyte subpopulation and its relationship to the prognosis of patients.

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