• 한국초등과학교육학회지:초등과학교육
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• 제19권1호
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• pp.145-156
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• 2000

Elementary school teachers' understandings about buoyancy were investigated through the questionnaire method. The questionnaire was composed of 4 questions on hydraulic pressure and 8 questions on buoyancy. The questions on buoyancy asked about the correlation of buoyancy with following basic concepts, density of liquid, volume of submerged object and so forth. 295 teachers on the 22 elementary schools in Busan, Yangsan and Gimhae were selected through random sampling method. The results of this study were summarized as follows: On the correlation of the magnitude and direction of hydraulic pressure with the depth of water, a large portion of the respondents had a scientific conception. But on the correlation of hydraulic pressure with density, the relatively small portion of them appeared to have a scientific conception. The respondents, on the whole, had a scientific conception about the correlation of buoyancy with density of liquid. But they seemed to have naive conceptions about the correlation of buoyancy with the volume of a submerged object and with the depth of water, the amount of water in container and the reduced amount of water by the object from container. We found that the respondents were context dependent and tended to search for solutions for the questions of buoyancy using the concept of pressure in the water. From above results, we suggested that in the would-be revised elementary science text book, the contents of pressure in the water should be introduced after the concept of weight in the water was gained.

• Journal of Microbiology and Biotechnology
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• 제14권1호
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• pp.15-21
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• 2004

The immunomodulating activities and chemical characteristics of a water-soluble exopolymer from submerged mycelial culture of Phellinus pini were studied. Anticomplementary activity of this polymer was found to be $73.2\%$, and its activation system occurred through both classical and alternative pathways, where the classical pathway was detected to be the major one by crossed immunoelectrophoresis. Nitric oxide (NO) release ability and acid phosphatase activity of macrophage were increased by 1.6-fold ($100{\mu}g/ml$) and 3.4-fold ($500{\mu}g/ml$), respectively, and splenocyte proliferation in mixed lymphocyte reaction (MLR) was also increased by 2.6-fold ($200{\mu}g/ml$), compared to the control. The molecular weight of this polymer, determined by HPLC, was under 5 kDa. Total sugar and protein contents were 89.7 and 10.3%, respectively. Both sugar and amino acid compositions of the exopolymer were also analyzed.

• 한국군사과학기술학회지
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• 제19권2호
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• pp.227-235
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• 2016

Heavy-weight high speed underwater vehicle(HSUV) is launched from the submerged mother ship. For the safety point of view, it is important to confirm whether the HSUV would touch the launching mother ship. In this paper, the hydrodynamic force and moment were modeled by the polynomials of motion variables and the simple lift and drag acting on a plate and cylinder which consist of the HSUV's several parts. The mother ship was assumed as the Rankine half body to consider the flow field near the moving ship. Such hydrodynamic force and moment were included in the 6 DOF equations of motion of the HSUV and the dynamic simulations for the various conditions of the HSUV until the propeller activation were performed. Developed simulation program is expected to reduce the number of expensive sea trial test to develop safety logic of the HSUV at the initial firing stage.

• International Journal of Naval Architecture and Ocean Engineering
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• 제6권1호
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• pp.60-73
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• 2014

The seaward reverse wave, occurring on the submerged dual porous horizontal plate, can contribute to the reduction of the transmitted wave as it reflects the propagating wave. However, the collision between the propagating and seaward reverse waves increases the water level and acts as a weight on the horizontal plate. This study investigated the characteristics of the wave pressure created by the seaward reverse wave through the analysis of experimental data. The analysis confirmed the following results: 1) the time series of the wave pressure showed reverse phase phenomena due to the collision, and the wave pressures acted simultaneously on both upper and lower surfaces of the horizontal plate; 2) the horizontal plate became repeatedly compressed and tensile before and after the occurrence of the seaward reverse wave; and 3) the seaward reverse wave created the total wave pressure to the maximum towards the direction of gravity, primarily on the upper plate. It was also confirmed that the wave distributions showed a similar trend to the wave steepness. Such outcome of the analysis will provide basic information to the structural analysis of the horizontal plate as a wave dissipater of the steel-type breakwater (STB).

• 한국버섯학회지
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• 제21권3호
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• pp.110-117
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• 2023

Cordycepin (3'-deoxyadenosine) is a nucleoside analog known for its diverse range of biological activities. This study investigated the effect of different types of sawdust on the production of the bioactive compound cordycepin. The results of the study showed that different types of wood sawdust affected the biosynthesis of cordycepin and a significant increase was observed when the conventional SDB medium was replaced with 1% NaOH treated pine sawdust. To optimize cordycepin production from Paecilomyces tenuipes in a medium containing 1% NaOH-pretreated pine sawdust, we employed Response Surface Methodology (RSM) in its Box-Behnken design (BBD) canonical form. The optimal conditions were determined as follows: a particle size of 109.5111-mesh (140 ㎛) for 1% NaOH-pretreated pine sawdust, an input weight of 21.1679 g/L, and an incubation time of 73.8423 hours. According to our model, this combination is expected to yield a maximum cordycepin content of 896.1428 ㎍/mL. Experimental validation of this prediction was performed using the suggested optimal conditions, resulting in an average cordycepin content of 922.6771 ㎍/mL across three replicates, thus confirming the model's accuracy.

• KSBB Journal
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• 제21권2호
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• pp.103-109
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• 2006

H. erinaceum의 기능성 소재화 연구의 일환으로, 지금까지 체계적으로 연구된 바 없는 H. erinaceum의 액체배양을 시도하고. 이 버섯의 서로 다른 생물반응기하의 액체배양 특성을 규명하고 대량생산을 검토하였다. 5 L 용량의 jar fermenter와 air-lift fermenter에서의 액체배양의 비교실험을 통해 air-lift fermenter의 사용이 종균 배양에 적합한 것으로 나타났으며, 형태 분석을 통해 적정 크기($2{\sim}4mm$)의 pellet 형태의 유지가 대량 생산의 중요인자인 것을 규명하였다. Air-lift fermenter 하의 배양을 종균으로 하고, 적정 크기의 pellet을 유지하는 500 L까지의 jar fermenter를 사용한 회분 배양의 scale-up 실험결과, 30 및 500 L에서의 교반 및 통기속도는 각각 200 rpm, 1 vvm 및 150 rpm, 1 vvm이었다. 이 조건하에서 배양의 전 기간 중 적정 pellet을 유지하였으며, 최대 균사체량은 약 $13{\sim}14g/L$이었다.

• 한국농공학회지
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• 제36권4호
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• pp.33-38
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• 1994

Floating-type automatic turnout was developed for the purpose of reducing labor cost and labor-working hours related to turnout management. The point of automation is to use a flexible-float within the turnout. The weight of float is changed by emptying and filling with water at the beginning and ending of irrigation. The turnout is controlled to open and close small bole on the float bottom using electromagnets. With the weight control of float. the gate of turnout is opened by the empty float to begin irrigatiom and is closed by the filled float to stop irrigation. The turnout was designed to be operated by the main computer and to minimize electric power consumption by sending an electric current at the beginning and ending of irrigation. The functional experiment was succesfully carried out and the rating curves for both free overflow condition and submerged flow condition were derived.

• 한국건축시공학회:학술대회논문집
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• 한국건축시공학회 2011년도 추계 학술논문 발표대회
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• pp.199-200
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• 2011

Absorption of lightweight aggregate affects the properties of fresh and hardened concrete, so care must be taken. In this study, according to KS F 2533 absorption is measured to aggregate size, submerged time, holding time and practically ways to maintain a constant absorption was to seek. The findings for quality control of the lightweight aggregate concrete mixture is saturation of the surface-dry aggregate humidity 100% after 24 hours immersion in the environment has been stored for more than two days to absorb the state was able to define. Dry density at the surface of the lightweight aggregate and lightweight concrete mix design and placement is possible for it to apply.

• 한국식품저장유통학회:학술대회논문집
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• 한국식품저장유통학회 2007년도 학술발표회
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• pp.27-31
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• 2007

This study relates to low and medium molecular weight isoflavone-${\beta}$-D-glucan produced by submerged liquid culture of Agaricus blazei, a method of producing the isoflavone-B-D-glucan using autolysis enzyme of Agaricus blazei mycelia, and use of the isoflavone-B-D-glucan for anti-cancer and immunoenhancing effect. In acordance with one aspect of the present study, it deals with a method of producing isoflavone-${\beta}$-D-glucan, which comprises the followings; 1) culturing and separating mushroom mycelia, 2) producing low-medium molecular weight isoflavone-${\beta}$-D-glucan from high molecular weight one. The cytotoxicity on human cnacer cell line (Caco-2, MCF-7), the expression of Cyclin D, Bcl-2, Bax protein, p21 protein, p53 protein in MCF-7 cells assessed by SDS-PAGE and immunoblotting, and other immuno related factors such as TNF-${\alpha}$ and IL-1B activities were examined. Structural identification of isoflavone-${\beta}$-D-glucan which showed cytotoxicity against cancer cell and immunoenhancing effects was carried by separation with DEAE-cellulose column chromatography, TLC, HPLC, IR, NMR. Clinical test for the cancer patients (n=119) for 6 month was carried out, and immunoenhancing factors (NK. cell number, ratio of T4/T8) were checked. We concluded the identified isoflavone-${\beta}$-D-glucan has immuno enhancing effects and could be useful for cancer chemoprevention.

• 식품저장과 가공산업
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• 제6권2호
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• pp.11-13
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• 2007

This study relates to low and medium molecular weight isoflavone-${\beta}$-D-glucan produced by submerged liquid culture of Agaricus blazei, a method of producing the isoflavone-B-D-glucan using autolysis enzyme of Agaricus blazei mycelia, and use of the isoflavone-B-D-glucan for anti-cancer and immunoenhancing effect. In acordance with one aspect of the present study, it deals with a method of producing isoflavone-${\beta}$-D-glucan, which comprises the followings; 1) culturing and separating mushroom mycelia, 2) producing low-medium molecular weight isoflavone-${\beta}$-D-glucan from high molecular weight one. The cytotoxicity on human cnacer cell line (Caco-2, MCF-7), the expression of Cyclin D, Bcl-2, Bax protein, p21 protein, p53 protein in MCF-7 cells assessed by SDS-PAGE and immunoblotting, and other immuno related factor such as TNF-a and IL-1B activities were examined. Structural identification of isoflavone-${\beta}$-D-glucan which shoed cytotoxicity against cancer cell and immunoenhancing effects was carried by separation with DEAE-cellulose column chromatography, TLC, HPLC, IR, NMR, Clinical test for the cancer patients (n=119) for 6 month was carried out, and immunoenhancing factors(NK cell number, ratio of T4/T8) were checked. We concluded the identified isoflavone-${\beta}$-D-glucan has immuno enhancing effects and could be useful for cancer chemoprevention.