• 생약학회지
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• 제44권3호
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• pp.269-274
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• 2013

In this study, we investigated the anticancer effect of rice bran extract in HL-60 human promyelocytic leukemia cells. The extract of rice bran inhibited the proliferation of HL-60 cells. When treated with the rice bran extract, we could observe the apoptotic characteristics such as apoptotic bodies and the increase of sub-G1 hypodiploid cell population, increase of Bax level, decrease of Bcl-2 expression, cleavage of procaspase-3, cleavage of procaspase-9 and cleavage of poly(ADP-ribose) polymerase(PARP) in HL-60 cells. Furthermore, the apoptosis induction of HL-60 cells treated with the rice bran extract was also accompanied by the inactivation of mitogen-activated protein kinases (MAPK) such as ERK1/2 MAPK and p38 MAPK. In addition, the rice bran extract induced the down-regulation of c-myc. These data suggested that the rice bran extract could induce the apoptosis via the inactivation of ERK1/2 MAPK and p38 MAPK, and the down-regulation of c-myc in HL-60 acute pomyelocytic leukemia cells. The results support that the rice bran extract might have potential for the treatment of acute promyelocytic leukemia.

• The Journal of Korean Physical Therapy
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• 제15권1호
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• pp.155-170
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• 2003

Lumbar segmental instability is considered to represent a significant sub-group within the chronic low back pain population. This condition has a unique clinical presentation that displays its symptoms and movement dysfunction within the neutral zone of the motion segment. The loosening of the motion segment secondary to injury and associated dysfunction of the local muscle system renders it biomechanically vulnerable in the neutral zone. There in evidence of muscle dysfunction related to the control of the movement system. There is a clear link between reduced proprioceptive input, altered slow motor unit recruitment and the development of chronic pain states. Dysfunction in the global and local muscle systems in presented to support the development of a system of classification of muscle function and development of dysfunction related to musculoskeletal pain. The global muscles control range of movement and alignment, and evidence of dysfunction is presented in terms of imbalance in recruitment and length between the global stability muscles and the global mobility muscles. The local stability muscles demonstrate evidence of failure of aeequate segmental control in terms of allowing excessive uncontrolled translation or specific loss of cross-sectional area at the site of pathology Motor recruitment deficits present as altered timing and patterns of recruitment. The evidence of local and global dysfunction allows the development of an integrated model of movement dysfunction. The clinical diagnosis of this chronic low back pain condition is based on the report of pain and the observation of movement dysfunction within the neutral zone and the associated finding of excessive intervertebral motion at the symptomatic level. Four different clinical patterns are described based on the directional nature of the injury and the manifestation of the patient's symptoms and motor dysfunction. A specific stabilizing exercise intervention based on a motor learning model in proposed and evidence for the efficacy of the approach provided.

• Radiation Oncology Journal
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• 제34권2호
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• pp.128-134
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• 2016

Purpose: To evaluate the clinical outcomes of patients who underwent radiation therapy with or without targeted molecular therapy for the treatment of spinal metastasis from renal cell carcinoma (RCC). Materials and Methods: A total of 28 spinal metastatic lesions from RCC patients treated with radiotherapy between June 2009 and June 2015 were retrospectively reviewed. Thirteen lesions were treated concurrently with targeted molecular therapy (concurrent group) and 15 lesions were not (nonconcurrent group). Local control was defined as lack of radiographically evident local progression and neurological deterioration. Results: At a median follow-up of 11 months (range, 2 to 58 months), the 1-year local progression-free rate (LPFR) was 67.0%. The patients with concurrent targeted molecular therapy showed significantly higher LPFR than those without (p = 0.019). After multivariate analysis, use of concurrent targeted molecular therapy showed a tendency towards improved LPFR (hazard ratio, 0.13; 95% confidence interval, 0.01 to 1.16). There was no difference in the incidence of systemic progression between concurrent and nonconcurrent groups. No grade ${\geq}2$ toxicities were observed during or after radiotherapy. Conclusion: Our study suggests the possibility that concurrent use of targeted molecular therapy during radiotherapy may improve LPFR. Further study with a large population is required to confirm these results.

• Biomolecules & Therapeutics
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• 제20권3호
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• pp.273-279
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• 2012

Hesperidin, a flavanone present in citrus fruits, has been studied as potential therapeutic agents that have anti-tumor activity and apoptotic effects in several cancers, but there is no report about the apoptotic effect of hesperidin in human malignant pleural mesothelioma through the specificity protein 1 (Sp1) protein. We investigated whether hesperidin inhibited cell growth and regulated Sp1 target proteins by suppressing the levels of Sp1 protein in MSTO-211H cells. The $IC_{50}$ value of hesperidin was determined to be 152.3 ${\mu}M$ in MSTO-211H cells for 48 h. Our results suggested that hesperidin (0-160 ${\mu}M$) decreased cell viability, and induced apoptotic cell death. Hesperidin increased Sub-$G_1$ population in MSTO-211H cells. Hesperidin significantly suppressed mRNA/protein level of Sp1 and modulated the expression level of the Sp1 regulatory protein such as p27, p21, cyclin D1, Mcl-1, and survivin in mesothelioma cells. Also, hesperidin induced apoptotic signaling including: cleavages of Bid, caspase-3, and PARP, upregulation of Bax, and down-regulation of Bcl-$_{xl}$ in mesothelioma cells. These results show that hesperidin suppressed mesothelioma cell growth through inhibition of Sp1. In this study, we demonstrated that Sp1 acts as a novel molecular target of hesperidin in human malignant pleural mesothelioma.

• Journal of Preventive Medicine and Public Health
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• 제42권6호
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• pp.392-402
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• 2009

Objectives : The aims of this paper were to develop the composite deprivation index (CDI) for the sub-district (Eup-Myen-Dong) levels based on the theory of social exclusion and to explore the relationship between the CDI and the standardized mortality ratio (SMR). Methods : The paper calculated the age adjusted SMR and we included five dimensions of social exclusion for CDI; unemployment, poverty, housing, labor and social network. The proxy variables of the five dimensions were the proportion of unemployed males, the percent of recipients receiving National Basic Livelihood Security Act benefits, the proportion of households under the minimum housing standard, the proportion of people with a low social class and the proportion of single-parent household. All the variables were standardized using geometric transformation and then we summed up them for a single index. The paper utilized the 2004-2006 National Death Registry data, the 2003-2006 national residents' registration data, the 2005 Population Census data and the 2005-2006 means-tested benefit recipients' data. Results : The figures were 115.6, 105.8 and 105.1 for the CDI of metropolitan areas (big cities), middle size cities and rural areas, respectively. The distributional variation of the CDI was the highest in metropolitan areas (8.9 - 353.7) and the lowest was in the rural areas (26.8 - 209.7). The extent and relative differences of deprivation increased with urbanization. Compared to the Townsend and Carstairs index, the CDI better represented the characteristics of rural deprivation. The correlation with the SMR was statistically significant and the direction of the CDI effects on the SMR was in accordance with that of the previous studies. Conclusions : The study findings indicated mortality inequalities due to the difference in the CDI. Despite the attempt to improve deprivation measures, further research is warranted for the consensus development of a deprivation index.

• 한국측량학회지
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• 제23권2호
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• pp.129-136
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• 2005

최근 과학기술의 발달과 급속한 도시화, 산업화 등으로 토지활용에 대한관심이 증가됨에 따라 토지의 이용이 지표면뿐만 아니라 지상의 공간과 지하의 공간으로 확대되고 있다. 이와 같은 토지이용의 입체화에 따라 공간상의 권리의 대상인 각종 건축물 및 시설물 등을 현재의 2차원 지적등록 방법으로는 그 한계성을 나타내고 있다. 따라서 지표면은 물론 공간상에 생성될 수 있는 다양한 형태의 구조물 및 시설물 등의 물리적 객체와 소유권 및 기타 권리관계를 등록할 수 있는 방안이 필요하게 되었다. 본 연구에서는 3차원 지적등록의 필요성과 3차원 지적등록의 대상 등의 분석을 통하여 공간상의 3차원 지적 분할과 이의 가시화를 통하여 실현가능한 3차원 지적 등록을 위한 모형을 제시하고자 하였다.

• 한국수자원학회:학술대회논문집
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• 한국수자원학회 2019년도 학술발표회
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• pp.407-407
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• 2019

According to Asian Development Bank report Pakistan is among water scarce countries. Climate scenario on the basis IPCC fifth assessment report (AR5) revealed that annual mean temperature of Pakistan from year 2010-2019 was $17C^o$ which will rise up to $21C^o$ at the end of this century, similarly almost 10% decrease of annual rainfall is expected at the end of the century. It is a changing task in underdeveloped countries like Pakistan to meet the water demands of rapidly increasing population in a changing climate. While many studies have tackled scarcity and stream flow forecasting of the Upper Indus Basin (UIB) Pakistan, very few of them are related to socio-economic and climate change impact on sustainable water management of UIB. This study investigates the pattern of current and future surface water availability for various demand sites (e.g. domestic, agriculture and industrial) under different socio-economic and climate change scenarios in Upper Indus Basin (UIB) Pakistan for a period of 2010 to 2050. A state-of-the-art planning tool Water Evaluation and Planning (WEAP) is used to analyze the dynamics of current and future water demand. The stream flow data of five sub catchment (Astore, Gilgit, Hunza, Shigar and Shoyke) and entire UIB were calibrated and validated for the year of 2006 to 2011 using WEAP. The Nash Sutcliffe coefficient and coefficient of determination is achieved ranging from 0.63 to 0.92. The results indicate that unmet water demand is likely to increase severe threshold and the external driving forces e.g. socio-economic and climate change will create a gap between supply and demand of water.

• 국제지역연구
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• 제18권3호
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• pp.23-46
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• 2014

효율적인 외국인직접투자유치를 위해서는 투자의 증감요인을 적기에 파악하는 것이 중요하다. 본 논문은 선진국그룹을 모집단으로 한 동태적 변이-할당분석(dynamic shift-share analysis)을 실시하여 2003-2012년 기간 중 한국으로 유입된 외국인직접투자의 증감을 세계성장효과, 산업구성효과, 경쟁력효과 등의 3개 요인으로 분해한다. 또한 분석결과를 세계를 모집단으로 한 분석결과 및 일반적인 변이-할당분석 결과와 비교한다. 2003-2012년 기간 중 대한(對韓) 외국인직접투자의 증가세는 선진국그룹의 추세보다 낮은 수준이었으며, 그 결과 산업구성효과와 경쟁력효과는 전체적으로 부(-)의 효과를 기록한 것으로 나타났다. 산업별로는 섬유 직물 의류, 화공, 전기 전자, 운송용 기계, 음식 숙박, 비즈니스서비스 산업이 양(+)의 경쟁력효과를 나타냈다. 분석기간을 2003-2007년 및 2008-2012년의 2개 기간으로 구분하면 2003-2007년 기간에는 대부분의 업종이 부의 경쟁력효과를 나타냈으나 2008-2012년 기간에는 대부분의 제조업 세부업종들이 양의 경쟁력효과를 나타냈다. 이는 한국으로 유입되는 외국인직접투자가 금융위기의 영향을 비교적 적게 받은 것을 반영한 것으로 해석된다.

• Annals of Surgical Treatment and Research
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• 제95권5호
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• pp.240-248
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• 2018

Purpose: This study aimed to validate the synergistic effect of ABT-737 on docetaxel using MDA-MB-231, a triple negative breast cancer (TNBC) cell line overexpressing B-cell lymphoma-2 (Bcl-2). Methods: Western blot analysis was performed to assess expression levels of Bcl-2 family proteins and caspase-related molecules. Cell viability was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Cell cycle distribution was determined by flow cytometry analysis. Benzyloxycarbonyl-Val-Ala-Asp(OMe)-fluoromethylketone (z-VAD-fmk) was used for pretreatment to assess the role of caspases. Results: Cell viability of MDA-MB-231 after combination treatment with ABT-737 and docetaxel was significantly lower than that after docetaxel or ABT-737 monotherapy based on MTT assay (both P < 0.001), with a combination index of 0.41. The proportion of sub-G1 population after combination treatment was significantly higher than that after docetaxel or ABT-737 monotherapy (P = 0.001, P = 0.003, respectively). Pretreatment with z-VAD-fmk completely restored cell viability of MDA-MB-231 from apoptotic cell death induced by combination therapy (P = 0.001). Although pro-caspase-8 or Bid did not show significant change in expression level, pro-casepase-9 showed significantly decreased expression after combination treatment. Cleaved caspase-3 showed increased expression while poly (ADP-ribose) polymerase cleavage was induced after combination treatment. However, hypoxia-inducible factor 1-alpha and aldehyde dehydrogenase 1 totally lost their expression after combination treatment. Conclusion: Combination of ABT-737 with docetaxel elicits synergistic therapeutic effect on MDA-MB-231, a TNBC cell line overexpressing Bcl-2, mainly by activating the intrinsic pathway of apoptosis. Therefore, adjunct of ABT-737 to docetaxel might be a new therapeutic option to overcome docetaxel resistance of TNBCs overexpressing Bcl-2.

• Parasites, Hosts and Diseases
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• 제56권6호
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• pp.625-632
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• 2018

Malaria remains one of the leading health burdens in the developing world, especially in several sub-Saharan Africa countries; and Uganda has some of the highest recorded measures of malaria transmission intensity in the world. It is evident that the prevalence of malaria infection, the incidence of disease, and mortality from severe malaria remain very high in Uganda. Although the recent stable political and economic situation in the last few decades in Uganda supported for a fairly good appreciation of malaria control, the declines in infection, morbidity, and mortality are not sufficient to interrupt transmission and this country is among the top 4 countries with cases of malaria, especially among children under 5 years of age. In fact, Uganda, which is endemic in over 95% of the country, is a representative of challenges facing malaria control in Africa. In this study, we evaluated an active case detection program in 6 randomly selected villages, Uganda. This program covered a potential target population of 5,017 individuals. Our team screened 12,257 samples of malaria by active case detection, every 4 months, from February 2015 to January 2017 in the 6 villages (a total of 6 times). This study assessed the perceptions and practices on malaria control in Kiyuni Parish of Kyankwanzi district, Uganda. Our study presents that the incidence of malaria is sustained high despite efforts to scale-up and improve the use of LLINs and access to ACDs, based on the average incidence confirmed by RDTs.