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Determination of Major Phenolic Compounds of Grape Juice and Wine of Different Geographic Origins (국내외 포도즙 및 포도주의 주요 페놀계 화합물 함량 비교분석)

  • Chang, Seog-Won;Song, Jeong-Hee;Shin, Nam-Sub;Lee, Ki-Yeol;Rho, Yong-Taek
    • Food Science and Preservation
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    • v.16 no.5
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    • pp.747-753
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    • 2009
  • Trans-resveratrol, quercetin, and epicatechin are natural polyphenolic substances with a wide range of biological activities, including anti-oxidant and anti-cancer properties. The compositions and levels of these chemicals were determined in grape (Vitis vinifera) juice and wine of different geographic origins. Significant differences in Hunter color values, pH, total acidity, and ethanol and free sugar contents, were found depending on both the nature and country of processing. Overall, trans-resveratrol and quercetin levels in wines were higher than in juices, indicating that wine is a rich source of these chemicals. Significant differences in the content and composition of epicatechin were found to depend on processing procedures, country of origin, and cultivar. This information may be useful to grape breeders and manufacturers of wine or juice.

A Study on Three-Dimensional Image Modeling and Visualization of Three-Dimensional Medical Image (삼차원 영상 모델링 및 삼차원 의료영상의 가시화에 관한 연구)

  • Lee, Kun;Gwun, Oubong
    • Journal of the Korea Computer Graphics Society
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    • v.3 no.2
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    • pp.27-34
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    • 1997
  • 3-D image modeling is in high demand for automated visual inspection and non-destructive testing. It also can be useful in biomedical research, medical therapy, surgery planning, and simulation of critical surgery (i.e. cranio-facial). Image processing and image analysis are used to enhance and classify medical volumetric data. Analyzing medical volumetric data is very difficult In this paper, we propose a new image modeling method based on tetrahedrization to improve the visualization of three-dimensional medical volumetric data. In this method, the trivariate piecewise linear interpolation is applied through the constructed tetrahedral domain. Also, visualization methods including iso-surface, color contouring, and slicing are discussed. This method can be useful to the correct and speedy analysis of medical volumetric data, because it doesn't have the ambiguity problem of Marching Cubes algorithm and achieves the data reduction. We expect to compensate the degradation of an accuracy by using an adaptive sub-division of tetrahedrization based on least squares fitting.

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Effectiveness of Pavement Marking for Speed Reduction by Using Driving Simulator (시뮬레이터를 이용한 감속유도 노면표시의 효과 연구)

  • Noh, Kwan-Sub;Lee, Jong-Hak;Kim, Jong-Min;Jang, Hye-Ran
    • International Journal of Highway Engineering
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    • v.12 no.3
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    • pp.9-16
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    • 2010
  • The purpose of this study is to establish how drivers react on different pavement markings in a driving simulator for speed reduction. For this, forty one people between the ages of twenty and seventy took part in this experiment. A study analyzed which patterns are effective to reduce a speeding. As a results, 'Peripheral Transverse Lines II' were the most effective treatment for reducing driving speeds in the driving simulator. This study carries an important meaning when presenting the effectiveness of speed reduction for perceptual countermeasure based on the human factors. Further research will be required to determine the long term effectiveness of these countermeasures due to concerns over drivers becoming too accustomed to implemented markings. Also, the color contrasts (for example, dark asphalt with white markings) can be considered.

Esterase isozyme patterns in developing plant regeneration from calli of citrus junos Sieb.

  • Lee, Hyun-Hwa;Lee, Sook-Young;Park, Min-Hee;Jang, Hyun-kyu;Kim, Hong-Sub
    • Plant Resources
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    • v.2 no.1
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    • pp.1-9
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    • 1999
  • The callus from the hypocotyl region of immature embryo of Citrus junos Sieb. was efficiently induced in the $\frac{1}{2}$ MS medium containing 45uM BA after 8 weeks culture. The callus was developed into the two callus type, embryogenic callus and nonembryogenic callus, which can be distinguished by visual examination depending on color and appearence. In vitro regeneration of callus established efficiently in the hormone-free MS medium from the embryogenic callus. In order to investigate the physiological changes depending on the developmental stage of embryo, the embryo was formed in the MS medium. The embryogenic and nonembryogenic callus, and the various stages of the somatic embryo were examimed the changes of esterase activity, and their isozyme patterns as well. The protein content and esterase activities was gradually increased on the developmental stages of embryo. Total protein pattern were different by the SDS-PAGE and were appeared strong band of 23 KD in the torpedo stage. The pattern of the esterase isozyme was exhibited a difference between embryogenic callus and nonembryogenic callus. It was appered pI 6.0, 8.0, 8.2 in the embryogenic callus. Also the new band of pI 4.75 was appeared in the cotyledon. These results suggest that the changes of esterase activities and isozyme patterns are importent factor in the differentiation and development of citrus.

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A Study on the Rise and Success of Korean-style Collection RPG Genre (한국형 수집 RPG 장르 형성 연구)

  • Cho, Eun-Ha
    • The Journal of the Korea Contents Association
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    • v.18 no.11
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    • pp.327-337
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    • 2018
  • The Korean-style Collection RPG is a unique sub-genre of RPG developed in a new environment of the smartphone era. Based on CCG in Japan, the Korean collection RPG was able to have its own color by adding action style based on the experience of MMORPG development. Since the genre, many games have been released and have had amazing success. This success is not only limited to domestic but also extends overseas. In this study, I try to find out the reasons for the emergence and success of the Korean collection RPG, and analyze "Seven Knights", which has been a great success in Korea, and "Summoners War", which has achieved great success in the world. Through this, I will contribute to the competitiveness of Korean smartphone game industry.

A LAMP-SNP Assay Detecting C580Y Mutation in Pfkelch13 Gene from Clinically Dried Blood Spot Samples

  • Khammanee, Thunchanok;Sawangjaroen, Nongyao;Buncherd, Hansuk;Tun, Aung Win;Thanapongpichat, Supinya
    • Parasites, Hosts and Diseases
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    • v.59 no.1
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    • pp.15-22
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    • 2021
  • Artemisinin resistance (ART) has been confirmed in Greater Mekong Sub-region countries. Currently, C580Y mutation on Pfkelch13 gene is known as the molecular marker for the detection of ART. Rapid and accurate detection of ART in field study is essential to guide malaria containment and elimination interventions. A simple method for collection of malaria-infected blood is to spot the blood on filter paper and is fast and easy for transportation and storage in the field study. This study aims to evaluate LAMP-SNP assay for C580Y mutation detection by introducing an extra mismatched nucleotide at the 3' end of the FIP primer. The LAMP-SNP assay was performed in a water bath held at a temperature of 56℃ for 45 min. LAMP-SNP products were interpreted by both gel-electrophoresis and HNB-visualized changes in color. The method was then tested with 120 P. falciparum DNA from dried blood spot samples. In comparing the LAMP-SNP assay results with those from DNA sequencing of the clinical samples, the 2 results fully agreed to detect C580Y. The sensitivity and specificity of the LAMP-SNP assay showed 100%. There were no cross-reactions with other Plasmodium species and other Pfkelch13 mutations. The LAMP-SNP assay performed in this study was rapid, reliable, and useful in detecting artemisinin resistance in the field study.

Comparison of Meat Quality Characteristics and Proteolysis Trends associated with Muscle Fiber Type Distribution between Duck Pectoralis Major and Iliotibialis Muscles

  • Cheng, Huilin;Song, Sumin;Park, Tae Sub;Kim, Gap-Don
    • Food Science of Animal Resources
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    • v.42 no.2
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    • pp.266-279
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    • 2022
  • This study was conducted to evaluate the proteolysis trends and change in meat quality during 10 days of cold storage in duck M. pectoralis major (PM) and M. iliotibialis (IL). Duck IL had a higher pH and greater degree of lightness but lower cooking loss than PM (p<0.05). During the 10-day cold storage, the pH value of PM declined significantly (p<0.05), while the meat quality traits of IL were not affected by cold storage (p>0.05). In PM, the redness increased from day 1 to day 5, while cooking loss was lower on day 10 compared to day 5 (p<0.05). There were no significant differences in the activities of cathepsin B and proteasome 20S during cold storage (p>0.05). The activity of calpains declined gradually during 10 days of storage (p<0.05), and the activity of calpains in PM was higher than that in IL (p<0.05). A total of 5,155 peptides were detected and derived from 34 proteins of duck PM muscle, whereas 4,222 peptides derived from 32 proteins were detected from duck IL muscle. Duck PM muscle was composed only of fast type of muscle fiber, whereas IL muscle was composed of both slow and fast types. The proteins responsible for glycolysis or myofibrillar proteins were closely related to changes in meat color or water-holding capacity during cold storage. These results suggest that changes in meat quality characteristics during cold storage are closely related to protein degradation, which is also related to the distribution of muscle fiber types.

Characteristics of the Fruits and Flesh Softening Delay Induced by GA3 and Thidiazuron (TDZ) Treatment in 'Heukboseok' Grape ('흑보석' 포도의 GA3와 Thidiazuron(TDZ) 처리에 따른 과육 연화 지연 및 과실특성)

  • Jung, MyungHee;Lee, ByulHaNa;Park, YoSup;Oh, Jin Pyo;Kim, HeeSeob;Park, Hee-Seung
    • Horticultural Science & Technology
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    • v.33 no.2
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    • pp.186-195
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    • 2015
  • This research was conducted to investigate effect the plant growth regulators (PGRs) on the characteristics of fruit and flesh softening, using GA3 and thidiazuron (TDZ) treatments in 'Heukboseok' grapes. The total yield obtained under PGR treatment was 88.3% lower than the target production when a single treatment with $GA_3$ of low concentrations was used, but the expected yield was recovered by combined treatment with TDZ and $GA_3$. When harvested on the basis of color, the harvest rate up to 100 days after full bloom (DAFB) was low with the $GA_3$ single treatment, but was increased by the addition of TDZ, with the second TDZ mixed treatment being particularly effective. The soluble solids content in the PGR-treated samples demonstrated no significant changes after 90 DAFB, while the acidity content decreased rapidly starting from 90 DAFB. Measured on the basis of sugar and acidity content, maturity was reached much earlier in treated fruit than in the non-treated fruit. Firmness was maintained at a higher level until the final harvest time after PGR treatment compared to untreated grapes according to epidermis thickness and flesh density increase to activity cell division and expansion by $GA_3$ and TDZ. In particular, the fruit quality was improved based on the delay of softening in primary and secondary treatments of $25mg{\cdot}L^{-1}\;GA_3+2.5mg{\cdot}L^{-1}$ TDZ. The production of seedless fruit was difficult, even with the inclusion of TDZ, reaching the highest seedless rate of only 65.5%. Fruit cracking was rare, occurring at a rate of about 0.0~0.9% in all treatments. Accordingly, 'Heukboseok' grapes should be harvested within 100 days after full bloom (DBFB) before a rapid decrease of firmness, conferred by primary and secondary treatments with $25mg{\cdot}L^{-1}\;GA_3+2.5mg{\cdot}L^{-1}$ TDZ.

Effect of Sulfur Dioxide (SO2) Pad on the Quality of Dried Persimmons and Dried Persimmons Slices during Storage at Room Temperature (이산화황(SO2) 패드가 상온저장 중 곶감 및 감말랭이의 품질에 미치는 영향)

  • Oh, Sung-Il;Lee, Sugwang;Park, Hyowon;Kim, Chul-Woo;Lee, Uk
    • Journal of Korean Society of Forest Science
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    • v.107 no.1
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    • pp.71-80
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    • 2018
  • The effect of $SO_2$ treated pads (Sodium metabisulphate 0, 0.5, 1.0, 2.0 g/kg) on the quality of dried persimmons and dried persimmons slices were investigated. The $SO_2$ treated pads did not affect to weight, moisture loss rate, and soluble solid contents of dried persimmons and dried persimmons slices. The color change (${\Delta}E$) of dried persimmons and dried persimmons slices after storage for 8 weeks were the highest (value=6.0, 6.2) in control, whereas that was the lowest (value=4.8, 4.7) under $SO_2$ pad 2.0 g/kg condition, respectively. When we measured the browning degree of dried persimmons, they showed O.D. 0.65, 0.57, 0.29, and 0.18 in serial dilution treated pads with $SO_2$ pad 0, 0.5, 1.0, and 2.0 g/kg. The browning degree data from aforementioned dried persimmons after 8 weeks was similar to that from dried persimmons slices. The decay rate of dried persimmons and dried persimmons slices after storage for 8 weeks were the highest (value=33.3%, 36.7%) in control, whereas that was the lowest (value=3.3%, 6.7%) under $SO_2$ pad 2.0 g/kg condition, respectively. The concentration of residual $SO_2$ in dried persimmons and dried persimmons slices were detected within a safe range of 15.3~30.0 ppm. Therefore, the shelf-life of dried persimmons and dried persimmons slices were lengthened in $SO_2$ treated pads (especially in $SO_2$ pad 2.0 g/kg) for inhibiting of browning and decay.

Studies on the Psysio-Chemical Changes in Apple Fruits During the Storage Under Sub-atmospheric Pressure (감압(減壓)에 의(依)한 사과 저장중(貯藏中)의 생리화학적(生理化學的) 변화(變化)에 관(關)한연구(硏究))

  • Sohn, Tae-Hwa
    • Applied Biological Chemistry
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    • v.19 no.4
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    • pp.202-218
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    • 1976
  • In this experiment American Summer Pairman apple was selected as test fruit in order to study the effect of sub-atmospheric pressure storage in the apple. keeping a certain pressure condition, a new sub-atmospheric pressure system was designed and constructed in which fruits were stored at $25^{\circ}C$ under two different atmospheric pressure conditions such as Normal Atmospheric Pressure (NAP) and Sub-Atmospheric Pressure (SAP). Moreover, they were divided into plots of 5% and 0% of $CO_2$, on the basis of gas composition. Under these conditions, the amount of respiration and ethylene evolution, and the changes of intercellular gas composition and organic components were investigated throughout the storage. The results obtained are as follows: (1) The intercellular gas was exhausted so rapidly by the SAP treatment that the gas equilibrium in the tissues reached within 5 minutes. (2) The amount of respiration was found to be higher in plots of NAP than SAP, and under the conditions, controlling $CO_2$ content, plots of $CO_2$ 5% were lower in the amount of respiration than $CO_2$ 0%. The climateric rise was revealed more slowly in plots of the SAP than NAP. These results indicate that the SAP treatment was an efficient method for controlling the respiration of fruits. Furthermore, these results were also realized with the results of the respiratory quotient (R.Q) and intercellular gas composition. (3) Evolution of ethylene, the ripening hormone in plant, was shown the similar tendency to the climacteric pattern of respiration; at the stage of climacteric maximum, the maximun amount of ethylene was found earlier in plots of NAP than SAP, and post climacteric stage was prolonged in the plots of the SAP compared to those of the NAP. The ethylene concentration in tissue appeared lower in plots of the SAP than NAP, which might suggest that the SAP treatment was caused to restriction of ethylene evolution. (4) Effects of the SAP treatment mentioned hitherto were proved also with the test of the external appearance such as changes of color and freshness, firmness, rotting and weight loss. (5) In the investigation of organic components, vitamin C and organic acids varied less in plots of SAP than NAP. Specially, it was remarkable that the loss of malic acid was least decreased by the SAP treatment. These effects of the SAP treatment were distinctive in the changes of the ratios of malic and citric acid to total acid, and the ratios of free sugar to free acid.

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