• Journal of the Earthquake Engineering Society of Korea
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• v.22 no.2
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• pp.45-54
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• 2018

The seismically isolated nuclear power plants shall be designed for design basis earthquake (DBE) and considered to ensure safety against beyond design basis earthquake (BDBE). In order to limit the excessive displacement of the seismic isolation system of the seismically isolated structure, the moat is installed at a certain distance from the upper mat supporting the superstructure. This certain distance is called clearance to stop (CS) and is calculated from the 90th percentile displacement of seismic isolation system subjected to BDBE. For design purposes, the CS can be obtained simply by multiplying the median displacement of the seismic isolation system against DBE by scale factor with a value of 3. The DBE and BDBE used in this study were generated by using 30 sets of artificial earthquakes corresponding to the nuclear standard design spectrum. In addition, latin hyper cube sampling was applied to generate 30 sets of artificial earthquakes corresponding to maximum - minimum spectra. For the DBE, the median displacement and the 99th percentile displacement of the seismic isolation system were calculated. For the BDBE, the suitability of the scale factor was assessed after calculating the 90th percentile displacement of the seismic isolation system.

• Journal of Life Science
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• v.13 no.4
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• pp.383-389
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• 2003

A cDNA (SeMIPS) encoding myo-inositol 1-phosphate synthase has been isolated from developing sesame (Sesamum indicum L. cv. Dan-Baek) seeds and its structure and function analyzed. The SeMIPS protein was highly homologous with those from plant species (88-94%), while a much lower degree of sequence homology (60%) was found with that of human. The functional domains commonly found in MIPS protein were identified and their amino acid residues were compared with each other. Northern blot indicated that the expression of the SeMIPS gene might be organ-specifically regulated. A complementation assay based on a yeast mutant system confirmed that the SeMIPS gene encodes a myo-inositol 1-phosphate synthase (MIPS) of sesame by showing functional expression of the SeMIPS cDNA in the yeast mutants containing the disrupted INO1 gene.

• Korean Journal of Ecology and Environment
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• v.52 no.3
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• pp.210-220
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• 2019

Population growth and the increase of energy consumption due to civilization caused global warming. Temperature on the Earth rose about $0.7^{\circ}C$ for the last 100 years, the rate is accelerated since 2000. Temperature is a factor, which determines physiological action, growth and development, survival, etc. of the plant together with light intensity and precipitation. Therefore, it is expected that global warming would affect broadly geographic distribution of the plant as well as structure and function ecosystem. In order to understand the effect of global warming on the ecosystem, a study about the effect of temperature rise on germination and growth in the plant is required necessarily. This study was carried out to investigate the effects of experimental warming on the germination and growth of two oak species(Quercus mongolica and Q. serrata) in temperature gradient chamber(TGC). This study was conducted in control, medium warming treatment($+1.7^{\circ}C$; Tm), and high warming treatment ($+3.2^{\circ}C$; Th) conditions. The final germination percentage, mean germination time and germination rate of two oak species increased by the warming treatment, and the increase in Q. serrata was higher than that in Q. mongolica. Root collar diameter, seedling height, leaf dry weight, stem dry weight, root dry weight, and total biomass were the highest in Tm treatment. Butthey were not significantly different in the Th treatment. In the Th treatment, Q. serrata had significantly higher H/D ratio, S/R ratio, and low root mass ratio (RMR) compared with control plot. Q. mongolica had lower RMR and higher S/R ratio in the Tm and Th treatments compared with control plot. Therefore, growth of Q. mongolica are expected to be more vulnerable to warming than that of Q. serrata. The main findings of this study, species-specific responses to experimental warming, could be applied to predict ecosystem changes from global warming. From the result of this study, we could deduce that temperature rise would increase germination of Q. serrata and Q. mongolica and consequently contribute to increase establishment rate in the early growth stage of the plants. But we have to consider diverse variables to understand properly the effects that global warming influences germination in natural condition. Treatment of global warming in the medium level increased the growth and the biomass of both Q. serrata and Q. mongolica. But the result of treatment in the high level showed different aspects. In particular, Q. mongolica, which grows in cooler zones of higher elevation on mountains or northward in latitude, responded more sensitively. Synthesized the results mentioned above, continuous global warming would function in stable establishment of both plants unfavorably. Compared the responses of both sample plants on temperature rise, Q. serrata increased germination rate more than Q. mongolica and Q. mongolica responded more sensitively than Q. serrata in biomass allocation with the increase of temperature. It was estimated that these results would due to a difference of microclimate originated from the spatial distribution of both plants.

• Korean Journal of Medicinal Crop Science
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• v.22 no.5
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• pp.339-348
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• 2014

The transcriptomes of four ginseng accessions such as Cheonryang (Korean ginseng cultivar), Yunpoong (Korean ginseng cultivar), G03080 (breeding line of Korean ginseng), and P. quinquefolius (American ginseng) was characterized. As a result of sequencing, total lengths of the reads in each sample were 156.42 Mb (Cheonryang cultivar), 161.95 Mb (Yunpoong cultivar), 165.07 Mb (G03080 breeding line), and 166.48 Mb (P. quinquefolius). Using a BLAST search against the Phytozome databases with an arbitrary expectation value of 1E-10, over 20,000 unigenes were functionally annotated and classified using DAVID software, and were found in response to external stress in the G03080 breeding line, as well as in the Cheonryang cultivar, which was associated with the ion binding term. Finally, unigenes related to transmembrane transporter activity were observed in Cheonryang and P. quinquefolius, which involves controlling osmotic pressure and turgor pressure within the cell. The expression patterns were analyzed to identify dehydrin family genes that were abundantly detected in the Cheonryang cultivar and the G03080 breeding line. In addition, the Yunpoong cultivar and P. quinquefolius accession had higher expression of heat shock proteins expressed in Ricinus communis. These results will be a valuable resource for understanding the structure and function of the ginseng transcriptomes.

• Journal of IKEEE
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• v.27 no.1
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• pp.116-121
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• 2023

In this paper, we develop a deep learning structure for a complex microbial incubator that applies deep learning prediction result information. The proposed complex microbial incubator consists of pre-processing of complex microbial data, conversion of complex microbial data structure, design of deep learning network, learning of the designed deep learning network, and GUI development applied to the prototype. In the complex microbial data preprocessing, one-hot encoding is performed on the amount of molasses, nutrients, plant extract, salt, etc. required for microbial culture, and the maximum-minimum normalization method for the pH concentration measured as a result of the culture and the number of microbial cells to preprocess the data. In the complex microbial data structure conversion, the preprocessed data is converted into a graph structure by connecting the water temperature and the number of microbial cells, and then expressed as an adjacency matrix and attribute information to be used as input data for a deep learning network. In deep learning network design, complex microbial data is learned by designing a graph convolutional network specialized for graph structures. The designed deep learning network uses a cosine loss function to proceed with learning in the direction of minimizing the error that occurs during learning. GUI development applied to the prototype shows the target pH concentration (3.8 or less) and the number of cells (10⁸ or more) of complex microorganisms in an order suitable for culturing according to the water temperature selected by the user. In order to evaluate the performance of the proposed microbial incubator, the results of experiments conducted by authorized testing institutes showed that the average pH was 3.7 and the number of cells of complex microorganisms was 1.7 × 10⁸. Therefore, the effectiveness of the deep learning structure for the complex microbial incubator applying the deep learning prediction result information proposed in this paper was proven.

• Applied Microscopy
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• v.39 no.2
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• pp.73-80
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• 2009

The plastid inclusion has long been known to exist in leaves of numerous plant species, especially in those of flowering plants. Among the inclusions, crystalline bodies are the most frequently distinguished structures of the foliar plastids, however, microtubules and phytoferritins are also reported occasionally. The crystalline inclusions vary in shape, and are located either in the stroma or within intrathylakoidal spaces, whereas microtubules and phytoferritins are more uniform in shape and are formed in the stroma. In crystalline structures, the composing elements exhibit a lattice pattern and/or paralleled tubules that are either bounded by membranes or exist without membrane enclosing. Other types of inclusions have not been shown to be enclosed by any membranous structures. According to the current survey, the plastid inclusion, with the exception of phytoferritins, has been shown to exhibit a crystalline or tubular pattern, and has been reported in more than 56 species of various families. Their occurrence is not restricted to any photosynthetic pathway, but is found to be randomly distributed among C-3, C-4 and CAM species, without phylogenetic relationships. The progress in plastid inclusion research reveals more information about the function and complexity, but the need for characterizing the 3-D structure of the crystalline inclusions also has been acknowledged in previous studies. A 3-D characterization would utilize tilting and tomography of serial sections with appropriate image processing that would provide valuable information on the sub-structures of the crystalline inclusions. In fact, recent studies performed on 3-D reconstruction of the plastid inclusions revealed important information about their comprising elements. In this article, the crystals and microtubules that have been reported in various types of plastids have been reviewed, with special consideration given to their possible sub-cellular function within the plastids.

• Journal of Plant Biotechnology
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• v.33 no.3
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• pp.153-160
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• 2006

Brassica rape is an important species used as a vegetable, oil, and fodder worldwide. It is related phylogenically to Arabidopsis thaliana, which has already been fully sequenced as a model plant. The 'Multinational Brassica Genome Project (MBGP)'was launched by the international Brassica community with the aim of sequencing the whole genome of B. rapa in 2003 on account of its value and the fact that it has the smallest genome among the diploid Brassica. The genome study was carried out not only to know the structure of genome but also to understand the function and the evolution of the genes comprehensively. There are two mapping populations, over 1,000 molecular markers and a genetic map, 2 BAC libraries, physical map, a 22 cDHA libraries as suitable genomic materials for examining the genome of B. rapa ssp. pekinensis Chinese cabbage. As the first step for whole genome analysis, 220,000 BAC-end sequences of the KBrH and KBrB BAC library are achieved by cooperation of six countries. The results of BAC-end sequence analysis will provide a clue in understanding the structure of the genome of Brassica rapa by analyzing the gene sequence, annotation and abundant repetitive DHA. The second stage involves sequencing of the genetically mapped seed BACs and identifying the overlapping BACs for complete genome sequencing. Currently, the second stage is comprises of process genetic anchoring using communal populations and maps to identify more than 1,000 seed BACs based on a BAC-to-BAC strategy. For the initial sequencing, 629 seed BACs corresponding to the minimum tiling path onto Arabidopsis genome were selected and fully sequenced. These BACs are now anchoring to the genetic map using the development of SSR markers. This information will be useful for identifying near BAC clones with the seed BAC on a genome map. From the BAC sequences, it is revealed that the Brassica rapa genome has extensive triplication of the DNA segment coupled with variable gene losses and rearrangements within the segments. This article introduces the current status and prospective of Korea Brassica Genome Project and the bioinformatics tools possessed in each national team. In the near future, data of the genome will contribute to improving Brassicas for their economic use as well as in understanding the evolutional process.

• Journal of Navigation and Port Research
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• v.39 no.6
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• pp.457-463
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• 2015

A gas turbine engine plays an important role as a prime mover that is used in the marine transportation field as well as the space/aviation and power plant fields. However, it has a complicated structure and there is a time delay element in the combustion process. Therefore, an elaborate mathematical model needs to be developed to control a gas turbine engine. In this study, a modeling technique for a gas generator, a PLA actuator, and a metering valve, which are major components of a gas turbine engine, is explained. In addition, sub-models are obtained at several operating points in a steady state based on the trial running data of a gas turbine engine, and a method for controlling the engine speed is proposed by designing an NPID controller for each sub-model. The proposed NPID controller uses three kinds of gains that are implemented with a nonlinear function. The parameters of the NPID controller are tuned using real-coded genetic algorithms in terms of minimizing the objective function. The validity of the proposed method is examined by applying to a gas turbine engine and by conducting a simulation.

• Journal of Korean Society of Coastal and Ocean Engineers
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• v.26 no.3
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• pp.174-183
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• 2014

Seabed beneath and near coastal structures may undergo large excess pore water pressure composed of oscillatory and residual components in the case of long durations of high wave loading. This excess pore water pressure may reduce effective stress and, consequently, the seabed may liquefy. If liquefaction occurs in the seabed, the structure may sink, overturn, and eventually increase the failure potential. In this study, to evaluate the liquefaction potential on the seabed, numerical analysis was conducted using the expanded 2-dimensional numerical wave tank to account for an irregular wave field. In the condition of an irregular wave field, the dynamic wave pressure and water flow velocity acting on the seabed and the surface boundary of the composite breakwater structure were estimated. Simulation results were used as input data in a finite element computer program for elastoplastic seabed response. Simulations evaluated the time and spatial variations in excess pore water pressure, effective stress, and liquefaction potential in the seabed. Additionally, the deformation of the seabed and the displacement of the structure as a function of time were quantitatively evaluated. From the results of the analysis, the liquefaction potential at the seabed in front and rear of the composite breakwater was identified. Since the liquefied seabed particles have no resistance to force, scour potential could increase on the seabed. In addition, the strength decrease of the seabed due to the liquefaction can increase the structural motion and significantly influence the stability of the composite breakwater. Due to limitations of allowable paper length, the studied results were divided into two portions; (I) focusing on the dynamic response of structure, acceleration, deformation of seabed, and (II) focusing on the time variation in excess pore water pressure, liquefaction, effective stress path in the seabed. This paper corresponds to (II).

• Journal of Korean Society of Coastal and Ocean Engineers
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• v.26 no.3
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• pp.160-173
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• 2014

Seabed beneath and near coastal structures may undergo large excess pore water pressure composed of oscillatory and residual components in the case of long durations of high wave loading. This excess pore water pressure may reduce effective stress and, consequently, the seabed may liquefy. If liquefaction occurs in the seabed, the structure may sink, overturn, and eventually increase the failure potential. In this study, to evaluate the liquefaction potential on the seabed, numerical analysis was conducted using the expanded 2-dimensional numerical wave tank to account for an irregular wave field. In the condition of an irregular wave field, the dynamic wave pressure and water flow velocity acting on the seabed and the surface boundary of the composite breakwater structure were estimated. Simulation results were used as input data in a finite element computer program for elastoplastic seabed response. Simulations evaluated the time and spatial variations in excess pore water pressure, effective stress, and liquefaction potential in the seabed. Additionally, the deformation of the seabed and the displacement of the structure as a function of time were quantitatively evaluated. From the results of the analysis, the liquefaction potential at the seabed in front and rear of the composite breakwater was identified. Since the liquefied seabed particles have no resistance to force, scour potential could increase on the seabed. In addition, the strength decrease of the seabed due to the liquefaction can increase the structural motion and significantly influence the stability of the composite breakwater. Due to limitations of allowable paper length, the studied results were divided into two portions; (I) focusing on the dynamic response of structure, acceleration, deformation of seabed, and (II) focusing on the time variation in excess pore water pressure, liquefaction, effective stress path in the seabed. This paper corresponds to (I).