• 전산구조공학
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• 제21권1호
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• pp.21-30
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• 2008

• Journal of Microbiology and Biotechnology
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• 제15권6호
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• pp.1250-1257
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• 2005

Two groups of exopolysaccharides (designated as Fr-I EPS and Fr-II EPS) were isolated from the culture filtrate of new fungal strain Trichoderma erinaceum DG-312 by Sepharose CL-6B chromatography. The structures of the exopolysaccharides were investigated using gas chromatography (GC), Fourier transform-infrared (FT-IR) spectroscopy, GCMS analysis, and NMR. GC analysis indicated that Fr-I EPS was composed of mainly mannose ($78.9\%$) and galactose ($21.1\%$), whereas Fr-II EPS contained mannose ($68.4\%$), galactose ($26.2\%$), and glucose ($5.4\%$). In the anomeric region ($950-700cm_{-1}$) of the FT-IR spectrum, both EPSs exhibited obvious characteristic absorption of $810\;cm_{-1}$, indicating the existence of mannose. The spectra of $\alpha-and\;\beta$-configurations were assigned at 880 and $914\;cm_{-1}$, respectively. The results of GC-MS analyses confirmed that both EPSs were complex heteropolysaccharides with a ($1{\rightarrow}3$)-linked mannan backbone. The C-1 region that appeared in the $^{13}C-NMR$ spectra of these EPSs indicated a typical anomeric carbon signal. The Fr-I EPS showed two anomeric carbon signals at 102.6 and 99.6 ppm, whereas the Fr-II EPS displayed four anomeric carbon signals at 102.5, 99.6, 98.5, and 94.3 ppm. The molecular characteristics of the EPSs were further investigated using a size exclusion chromatography/multi-angle laser light scattering (SEC/MALLS) system. The SEC/MALLS system revealed that the average molar masses of the EPSs were $6.592{\times}10^{4}$ (Fr-I EPS) and $1.920{\times}10^{4}$ (Fr-II EPS) g/mol, and the molecular conformation of both EPSs in aqueous solution was random coils.

• 한국전산구조공학회논문집
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• 제22권6호
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• pp.541-548
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• 2009

본 논문은 샤르피 충격실험과 유한요소법를 이용하여 노치위치에 따른 파괴거동과 흡수에너지의 영향을 평가하였다. 본 연구자는 쉴드메탈아크 용접방법으로 두께가 25mm인 압력용기용강(SA-516 Gr. 70)을 용접하였고, 이 용접된 평판으로 샤르피 시편을 제작하였다. 샤르피 충격실험에서는 용접열영향부(HAZ)에서 노치위치가 다른 시편이 사용되었다. 그리고 본 연구자는 유한요소법을 이용하여 샤르피 충격실험에서의 균열진전을 모사하였다. 용접열영향부(HAZ)의 기계적 물성을 유한요소해석에 적용하기 위해 HAZ를 2개 영역, 3개 영역 그리고 4개 영역으로 나누었다. 본 연구결과에서는 샤르피 충격실험의 흡수에너지가 노치위치에 의존적이라는 것을 보여주었다. 또한 샤르피 용접시편에서 신뢰성 있는 유한요소해석 결과를 얻기 위해서는 용접열영향부를 적어도 3개 이상의 영역으로 나누어야 한다는 결과를 얻었다.