• Korean Journal of Microbiology
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• v.27 no.4
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• pp.317-322
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• 1989

A gene can be selectively overexpressed in E. coli by utilizing the phage T7 RNA polymerase's stringent recognition and active transcription of the T7 promoter. The T7 expression system was constructed such that the T7 RNA polymerase gene is under the control of lacUV5 promoter in one plasmid, and that the target gene, the promoterless chloramphenicol acetyltransferase (CAT) gene with E. coli ribosome binding site is under the control of T7 promoter in the other plasmid. Only the E. coli cells containing both plasmids show high resistance to chloramphenicol. When the copy number of the runaway plasmid containing the polymerase gene was varied by a temperature shift, amounts of the CAT protein synthesized upon induction was correspondingly changed as shown in SDS gel electrophoresis.

• JSTS:Journal of Semiconductor Technology and Science
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• v.15 no.2
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• pp.280-285
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• 2015

A fully integrated high-efficiency linear CMOS power amplifier (PA) is developed for 802.11n WLAN applications using the 65-nm standard CMOS technology. The transformer topology is investigated to obtain a high-efficiency and high-linearity performance. By adopting a 2:2 output transformer, an optimum impedance is provided to the PA core. Besides, a LC harmonic control block is added to reduce the AM-to-AM/AM-to-PM distortions. The CMOS PA produces a saturated power of 26.1 dBm with a peak power-added efficiency (PAE) of 38.2%. The PA is tested using an 802.11n signal, and it satisfies the stringent error vector magnitude (EVM) and mask requirements. It achieves -28-dB EVM at an output power of 18.6 dBm with a PAE of 14.7%.

• Proceedings of the Acoustical Society of Korea Conference
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• 1998.06c
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• pp.351.1-355
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• 1998

Network-based real-time multimedia applications require guaranteed performances of communication service. Also according to increased bandwidth and processing performance in the future high speed networks, it is possible to make multimedia real-time applications such as distributed real-time applications including video conference, medical imaging, and video education. These applications have characteristics of stringent performance requirements in terms of delay, delay jitter and maximum loss rate. This paper proposes a new delay control for Broadband networks with FCFS queueing. The delay control provides an alternative solution to a current problem in real-time communication; traffic distortion, which makes it difficult to meet such real-time requirements. In simulations, it shows that the proposed delay control can guarantee local and end-to-end delay.

• Environmental Engineering Research
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• v.21 no.2
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• pp.109-120
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• 2016

Conventional treatment techniques cannot meet the stringent modern water quality regulations emanating from the need to provide high quality drinking water. Therefore, a number of studies have suggested low pressure membrane filtration as a worthwhile alternative. However, a major constraint to the extensive use of this technology in low and middle income countries is the high operating and maintenance costs caused by the inherent predisposition to membrane fouling. Notwithstanding, pretreatment of feed water using techniques such as coagulation, adsorption, oxidation and bio-filtration is believed to control fouling. In this review paper, the existing scientific knowledge on membrane fouling and pretreatment techniques for controlling fouling in low pressure membranes is analyzed with the aim of providing new and valuable insights into such techniques, as well as unveiling crucial issues noteworthy for further studies. Among the techniques reviewed, coagulation was observed to be the most cost-effective and will remain the most dominant in the coming years. Although oxidants and magnetic ion exchange resins can also control fouling, the propensity of oxidants to form health treating precursors and the high economic implications of magnetic ion exchange resins will hinder their adoption in developing countries.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.33 no.7B
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• pp.510-517
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• 2008

In wireless sensor networks, a number of nodes deployed in dense manner should be self-configured to establish a topology that provides communication and sensing coverage under stringent energy constraints. To establish an efficient topology, we propose the TRaffic-Aware Topology control (TRAT) algorithm that reduces energy dissipation by considering total amount of data flows in the network. Our algorithm controls the number of active nodes with traffic information and adjusts nodal transmission power by estimating amount of data flows. According to the result, the proposed algorithm shows about 30% better performance than the other methods in terms of energy efficiency.

• The Transactions of the Korean Institute of Power Electronics
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• v.19 no.1
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• pp.41-50
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• 2014

The rising penetration of renewable energy resulted in the development of grid-connected large-scale power plants. Therefore, grid stabilization, which depends on the system-type or grid of each country, plays an important role and has been strengthened by different grid codes. With this background, VDE-AR-N 4105 for photovoltaic (PV) systems connected to the low-voltage grid and the German Association of Energy and Water Industries (BDEW) introduced the medium-voltage grid code for connecting power plants to the grid and they are the most stringent certifications. In this paper, an optimal control strategy scheme for three-phase grid-connected PV system is enhanced with VDE-AR-N 4105 and BDEW grid code, where both active/reactive powers are controlled. Simulation and experimental results of 100kW PV inverter are shown to verify the effectiveness of the proposed implemental control strategy.

• Transactions of the Korean Society of Automotive Engineers
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• v.10 no.4
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• pp.15-22
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• 2002

As the emission regulations on the automobiles have been increasingly stringent, precise control of air/fuel ration is one of the most important issues on the gasoline engines. Although many researches have been carried out to identify the fuel transport phenomena in the port fuel injection gasolines, mixture preparation in the cylinder has not been fully understood due to the complexity of fuel film behavior, In this paper, the mixture preparation during cold engine start is studied by using a Fast Response Flame ionization Detector.(FRFID) In order to estimate the transportation of injected fuel from the intake port into cylinder, the wall wetting fuel model was used. The two coefficient($\alpha$,$\beta$) of the wall-wetting fuel model was determined from the measured fuel mass that was inducted into the cylinder at the first cycle after injection cut-in. $\alpha$( ratio of directly inducted fuel mass into cylinder from injected fuel mass) and $\beta$ (ratio of indirectly inducted fuel mass into cylinder from wall wetted fuel film on the wall) was increased with increasing cooling water temperature. To reduce a air/fuel ratio fluctuation during cold engine start, the appropriate fuel injection rate was obtained from the wall wetting fuel model. Result of air/fuel ratio control, air/fuel excursion was reduced.

• International Journal of Contents
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• v.2 no.2
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• pp.10-16
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• 2006

In this paper, we propose a predictive resource metric region (RMR) based radio resource metric estimation (RRME) mechanism, which utilizes a resource metric mapping function (RMMF), both of which permit efficient inter-working between the physical layer and higher layers for envisaging multimedia service applications over a CDMA communication system platform. The RMR can provide the acceptable resource region where QoS and acceptable link quality can be guaranteed with an achievable resource margin to be utilized in terms of capacity margin, the degree of confidence (DCL) of user, second-order statistics of Eb/Io. With predicted capacity margin and variance, DCL can deliver decision parameters with which an adaptive QoS based admission control can perform well taking capacity and resource availability into account in a dynamic and predictive manner. Combined with advanced techniques such as adaptive modulation or rate control and power control, the proposed mechanism can adjust the conventional stringent link quality information efficiently, and deliver accurate information of the resource availability. Thus, these can guarantee the maximization of resource utilization of multimedia service applications.

• Journal of Ocean Engineering and Technology
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• v.13 no.3B
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• pp.38-46
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• 1999

Increasing stringent emissions legislation and requirement of more effective energy used for diesel engine demand the fine control of the fuel injection system. Recently, the electromagnetic fuel injection control system for diesel engine is tried to realize the optimum diesel combustion by the feel back sensing as optical signal of combustion flame. The photo detectors were made for the feed back signal of electromagnetic fuel injection control for small DI diesel engine. Their abilities to detect defining combustion events were examined. By evaluating test results, it was shown that the wider acceptable optical range design of optical probe window face, and selection of installation position and installation method of detector were important point for improving sensing ability. The detector was shown to detect start and end of diffused combustion and maximum point of flame intensity impossible for pressure sensor, and also shown that the maximum point of flame intensity was 75% of accumulated heat release point within the experimental conditions.

• Journal of ILASS-Korea
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• v.16 no.3
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• pp.134-139
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• 2011

Recent research has focused on engine combustion technology as well as application of after-treatment in order to comply with emission regulation. However, it is much more efficient way to control emissions from engine itself and furthermore research on engine control will provide the direction of after-treatment technology in future. Furthermore, emission standard regulation for passenger diesel vehicles has been stringent compared to others and nano-particles will be included in EURO6 regulation in Europe and similar emission standard will be introduced in Korea. A 3.0 liter high speed diesel engine equipped with by CRDI system of 160MPa injection pressure, and an intake/exhaust system of V type 6 cylinder turbo-intercooler was applied. The injection duration and injection quantity, pilot injection types which are related to CRDI and air/fuel ratio control applied by EVGT were changed simultaneously. Standard experiment procedure constituted dilution apparatus and CPC system to collect nano-particles and these test results were compared with regulated materials of CO, HC, NOx and investigated their relations and characteristics of nano-particles.