• Journal of Food Hygiene and Safety
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• v.34 no.1
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• pp.73-86
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• 2019

Seafaring is an important occupation that requires stringent hand hygiene practices as a basic method for preventing food-borne illness and infectious diseases when the diseases occur on board. The purpose of this study is to provide fundamental data for the prevention of food-borne illness and infectious disease on the ship by investigating the level of hand hygiene practices and influencing factors. A total of 222 seafarers were surveyed at a seafarers' educational institution between July and August 2017. Their hand hygiene practice were examined by a modified method using the guidelines which are recommended by the World Health Organization, the Centers for Disease Control and Prevention, and Hand Hygiene Australia. The mean of hand hygiene practice was 47.97 out of 75 points. By category, the most frequent hand hygiene practice was measured as 4.04 on a 5-point scale as 'after working'. Factors affecting hand hygiene practices were ship tonnage relating to in job characteristics, exercise in health-related characteristics, and soap in relation to the characteristics of the hand hygiene environment on board. To improve hand hygiene among seafarers, it is necessary to raise awareness of hand hygiene among seafarers who work on small ships in particular, and to improve the systems of hand hygiene on ships with continuous education, hygiene practice evaluation and feedback.

• Journal of the Korea Society for Simulation
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• v.19 no.4
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• pp.291-299
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• 2010

As recent advances in science, technology and performance requirements of the weapons system are getting highly diversified and complex, the performance requirements also get stringent and strict. Moreover, the weapons system should be intimately connected with other systems such as watchdog system, command and control system, C4I system, etc. However, a tremendous amount of time, cost and risk being spent to acquire new weapons system, and not being diminished compared to the rapid pace of its development speed. Defense Modeling and Simulation(M&S) comes into the spotlight as an alternative to overcoming these difficulties as well as constraints. In this paper, we propose the development process of virtual test framework based on the synthetic environment as a tool to analyze the effectiveness of the weapons system of underwater engagement model. To prove the proposed concept, we develop the test-bed of virtual test using Delta3D simulation engine, which is open source S/W. We also design the High Level Architecture and Real-time Infrastructure(HLA/RTI) based Federation for the interoperation with heterogeneous simulators. The significance of the study entails (1)the rapid and easy development of simulation tools that are customized for the Korean Theater of War; (2)the federation of environmental entities and the moving equations of the combat entities to manifest a realistic simulation.

• Journal of Korean Society of Environmental Engineers
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• v.31 no.8
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• pp.673-678
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• 2009

High-rate phosphorous removal by PAC (poly aluminum chloride) coagulation of A2O effluent was investigate to meet the stringent requirement of wastewater discharge from municipal wastewater treatment plant. A series of jar tests were conducted to find optimum coagulation condition and to enhance removal efficiency. The optimum volumetric concentration of PAC was 30 ppm (2.81mol Al/mol P by mol ratio). Only 17.2% of soluble P was removed for 30 minutes' settling without PAC addition, while this increased to 30.3% by dosing 10ppm PAC. It even increased conspicuously from 49.3% to 88.4% by increasing PAC dose from 20 ppm to 30 ppm. 92.4% of total P was removed by 30 ppm PAC, and the effluent concentration (0.3 mg/L) was acceptable for discharge. The optimum value of coagulation time, settling time, and pH were 4minutes, 20 minutes, and 7.0, respectively. It was not necessary to control pH of raw sample whose pH was 7.0. Soluble P removal was remarkably enhanced at pH 7.0. This implied that sweep floc formation by $Al(OH)_3$ was the main mechanism of coagulation for soluble P removal. Influent and effluent of secondary clarifier were tested for coagulation, and the effluent was better for high-rate P removal. It resulted in 0.18 mg/L of P and 95.4% of P removal by coagulation. It was favorable to recycle the treated water to coagulation tank and the optimum recycle ratio was 0.3.

• Proceedings of the Korean Environmental Sciences Society Conference
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• 1998.10a
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• pp.2-4
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• 1998

Proliferation of Nocardia amarae cells in activated sludge has often been associated with the generation of nuisance foams. Despite intense research activities in recent years to examine the causes and control of Nocardia foaming in activated sludge, the foaming continued to persist throughout the activated sludge treatment plants in United States. In addition to causing various operational problems to treatment processes, the presence of Nocardia may have secondary effects on the fate of heavy metals that are not well known. For example, for treatment plants facing more stringent metal removal requirements, potential metal removal by Nocardia cells in foaming activated sludge would be a welcome secondary effect. In contrast, with new viosolid disposal regulations in place (Code o( Federal Regulation No. 503), higher concentration of metals in biosolids from foaming activated sludge could create management problems. The goal of this research was to investigate the metal sorption property of Nocardia amarae cells grown in batch reactors and in chemostat reactors. Specific surface area and metal sorption characteristics of N. amarae cells harvested at various growth stages were compared. Three metals examined in this study were copper, cadmium and nickel. Nocardia amarae strain (SRWTP isolate) used in this study was obtained from the University of California at Berkeley. The pure culture was grown in 4L batch reactor containing mineral salt medium with sodium acetate as the sole carbon source. In order to quantify the sorption of heavy metal ions to N amarae cell surfaces, cells from the batch reactor were harvested, washed, and suspended in 30mL centrifuge tubes. Metal sorption studies were conducted at pH 7.0 and ionlc strength of 10-2M. The sorption Isotherm showed that the cells harvested from the stationary and endogenous growth phase exhibited significantly higher metal sorption capacity than the cells from the exponential phase. The sequence of preferential uptake of metals by N. amarae cells was Cu>Cd>Ni. The specific surFace area of Nocardia cells was determined by a dye adsorption method. N.amarae cells growing at ewponential phase had significantly less specific surface area than that of stationary phase, indicating that the lower metal sorption capacity of Nocardia cells growing at exponential phase may be due to the lower specific surface area. The growth conditions of Nocardia cells in continuous culture affect their cell surface properties, thereby governing the adsorption capacity of heavy metal. The comparison of dye sorption isotherms for Nocardia cells growing at various growth rates revealed that the cell surface area increased with increasing sludge age, indicating that the cell surface area is highly dependent on the steady-state growth rate. The highest specific surface area of 199m21g was obtained from N.amarae cell harvested at 0.33 day-1 of growth rate. This result suggests that growth condition not only alters the structure of Nocardia cell wall but also affects the surface area, thus yielding more binding sites of metal removal. After reaching the steady-state condition at dilution rate, metal adsorption isotherms were used to determine the equilibrium distributions of metals between aqueous and Nocardia cell surfaces. The metal sorption capacity of Nocardia biomass harvested from 0.33 day-1 of growth rate was significantly higher than that of cells harvested from 0.5- and 1-day-1 operation, indicatng that N.amarae cells with a lower growth rate have higher sorpion capacity. This result was in close agreement with the trend observed from the batch study. To evaluate the effect of Nocardia cells on the metal binding capacity of activated sludge, specific surface area and metal sorption capacity of the mixture of Nocardia pure cultures and activated sludge biomass were determined by a series of batch experiments. The higher levels of Nocardia cells in the Nocardia-activated sludge samples resulted in the higher specific surface area, explaining the higher metal sorption sites by the mixed luquor samples containing greater amounts on Nocardia cells. The effect of Nocardia cells on the metal sorption capacity of activated sludge was evaluated by spiking an activated sludge sample with various amounts of pre culture Nocardia cells. The results of the Langmuir isotherm model fitted to the metal sorption by various mixtures of Nocardia and activated sludge indicated that the mixture containing higher Nocardia levels had higher metal adsorption capacity than the mixture containing lower Nocardia levels. At Nocardia levels above 100mg/g VSS, the metal sorption capacity of activate sludge increased proportionally with the amount of Noeardia cells present in the mixed liquor, indicating that the presence of Nocardia may increase the viosorption capacity of activated sludge.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.22 no.1
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• pp.431-438
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• 2021

To prepare more stringent regulations for USCG Phase II ballast water management, this study investigated the staining efficiency of SYBR Green I(SGI) and SYBR Gold(SG) on the virus-like particle (VLP). A dye with high staining efficiency was applied to the treated water that was passed through the ballast water treatment system (BWTS). VLP staining was observed most clearly under the 100-fold and 200-fold dilution of the stock solution when the volume of filtered samples was 0.5 mL to 2 mL. The staining efficiency of SGI and SG did not show a significant difference. On the other hand, the green fluorescence of viruses in the sample stained with SGI was more pronounced than in the samples stained with SG (expressed yellow fluorescence), making it easier to observe. The abundance of VLP in the test water and control water treatments that did not pass through the two types of BWTS (electrolysis type, UV + electrolysis type) was approximately 109 - 1010 VLP 100 mL-1. In contrast, no stained VLP was observed in the treated water treatments. Moreover, SGI was confirmed to be effectively stained under various salinity conditions, including seawater, brackish water, and freshwater. Further verification tests and development of staining methods under various BWTS are required, but the SGI staining method is believed to be a good alternative to the VLP live/dead determination of the USCG Phase II type approval test.