• Journal of Microbiology and Biotechnology
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• 제32권5호
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• pp.602-611
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• 2022

The persistence of pathogenic Escherichia coli under acidic conditions poses a serious risk to food safety, especially in acidic foods such as kimchi. To identify the bacterial factors required for acid resistance, transcriptomic analysis was conducted on an acid-resistant enterotoxigenic E. coli strain and the genes with significant changes in their expression under acidic pH were selected as putative resistance factors against acid stress. These genes included those associated with a glutamate-dependent acid resistance (GDAR) system and copper resistance. E. coli strains lacking GadA, GadB, or YbaST, the components of the GDAR system, exhibited significantly attenuated growth and survival under acidic stress conditions. Accordantly, the inhibition of the GDAR system by 3-mercaptopropionic acid and aminooxyacetic acid abolished bacterial adaptation and survival under acidic conditions, indicating the indispensable role of a GDAR system in acid resistance. Intriguingly, the lack of cueR encoding a transcriptional regulator for copper resistance genes markedly impaired bacterial resistance to acid stress as well as copper. Conversely, the absence of YbaST severely compromised bacterial resistance against copper, suggesting an interplay between acid and copper resistance. These results suggest that a GDAR system can be a promising target for developing control measures to prevent E. coli resistance to acid and copper treatments.

• 한국지반공학회:학술대회논문집
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• 한국지반공학회 2003년도 봄 학술발표회 논문집
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• pp.569-576
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• 2003

Based on the equivalent uniform stress concept Presented by Seed and Idriss, sinusoidal cyclic loads which simplified earthquake loads have been applied in evaluating the liquefaction resistance strength experimentally. However, the liquefaction resistance strength of soil based on the equivalent uniform stress concept can not exactly reflect the dynamic characteristics of the irregular earthquake motion. The liquefaction assessment method which was invented by using the equivalent uniform stress concept is suitable for the severe earthquake region such as Japan or USA, so the proper method to Korea is needed. In this study, estimation of the resistance to liquefaction was conducted by applying real earthquake loading to the cyclic triaxial test. From the test results, the characteristics of the fine sand under moderate earthquake were analyzed and compared with the results under strong earthquakes. Typically real earthquake loads used in this study are divided into two types - impact type and vibration type. Furthermore, results of the liquefaction resistance strength based on the equivalent uniform stress concept and tile concept using real earthquake loading were compared.

• 한국환경과학회지
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• 제21권3호
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• pp.267-276
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• 2012

Spaceflights results in the reduction of immune status of human beings and increase in the virulence of microorganisms, especially gram negative bacteria. The growth of Klebsiella pneumoniae is enhanced by catecholamines and during spaceflight, elevation in the levels of cortisols occurs. So it is necessary to know the changes in physiology, virulence, antibiotic resistance and gene expression of K. pneumoniae under microgravity conditions. The present study was undertaken to study effect of simulated microgravity on growth, morphology, antibiotic resistance and cross stress resistance of K. pneumoniae to various stresses. The susceptibility of simulated microgravity grown K. pneumoniae to ampicillin, penicillin, streptomycin, kanamycin, hygromycin and rifampicin were evaluated. The growth of bacteria was found to be fast compared with normal gravity grown bacteria and no significant changes in the antibiotic resistance were found. The bacteria cultured under microgravity conferred cross stress resistance to acid, temperature and osmotic stress higher than the normal gravity cultured bacteria but the vice versa was found in case of oxidative stress.

• Journal of the Korean Wood Science and Technology
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• 제31권6호
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• pp.45-54
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• 2003

The density and strength of wood is affected by degradations and defects, such as voids and knots. Old wooden structures such as traditional cultural properties have been deteriorated by these types of defects. They were evaluated by a visual observation that is difficult to evaluate the inner deterioration in structures. In this study, three nondestructive testing techniques were investigated to detect the wooden structural members. Ultrasonic stress wave tests, drilling resistance tests and visual inspections were used to examine the structural wood members. Patterns of Resistograph using by drilling resistance tests could indicate the features of internal wood such as voids, knots, decay, fungi, and so on. The technique just like as ultrasonic stress wave tests, however, difficult to detect exactly area where small amounts of internal deterioration in logs are. In spite of results of ultrasonic stress wave test, the internal deterioration of wooden structural members could be evaluated by the relationship between ultrasonic stress wave tests and drilling resistance tests.

• 한국전기전자재료학회논문지
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• 제22권1호
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• pp.67-73
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• 2009

The stress-strain behavior and its effects on the crack initiation and growth of ITO film on PET substrate with a sheet resistance of 45 ohms/sq were investigated. Electrical resistance increased gradually at the strain of 0.7% in the elastic to plastic transition region of the stress strain curves. Numerous cracks were observed after 1% strain and the increase of the resistance can be linked to the cracking of ITO thin films. The onset strain for the increase of resistance increased with increasing strain rate, suggesting the crack initiation is dependent on the strain rate. Upon loading, the initial cracks perpendicular to the tensile axis were observed and propagated the whole sample width with increasing strain. The spacing between horizontal cracks is thought to be determined by the fracture strength and the interfacial strength between ITO and PET. The crack density increased with increasing strain. The spacing between horizontal cracks (perpendicular to the stress axis) increased with decreasing strain rate, The increase of crack density with decreasing strain rate can be attributed to the higher fraction of the plastic strain to the total strain at a given total strain. As the strain increased over 5% strain, cracks parallel to the stress axis were developed and increased in number with strain, accompanied by drastic increases of resistance.

• 대한예방한의학회지
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• 제17권2호
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• pp.79-88
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• 2013

Objectives : This study investigated the relationship between smoking and health factors including stress resistance, stress index, fatigue and vaso-aging degree. Methods : The subjects were 19,953 persons who had received health examination by Korean Medicine and submitted questionnaires about smoking history, for 8 months from March to November in 2011. The stress index and resistance, fatigue were measured by HRV(Heart Rate Variability). The vaso-aging degree were measured by APG(Accelerated Photoplethysmograph). Results : Regarding the relationship between smoking and stress index and resistance, fatigue and vaso-aging degree were significant differences(p<0.05). Conclusions : This study suggests that smoking affects health factors including stress, fatigue and avaso-aging degree.

• 한국정밀공학회지
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• 제13권5호
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• pp.44-52
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• 1996

A procedure predicting warpage and post-deformation due to heat resistance test is presented. The procedure is applied to the injection molding processes of automotive plastic components, which are the door trim and the instrument pannel. The warpage of products is obtained from the residual stress after filling, packing and cooling process, and the post deformation due to the heat resistance test is calculated in the structural analysis of the product at the ejection temperature with the initial condition of residual stress, the boundary conditions and heat resistance conditions. The analyses give some useful guide lines in the design of automotive plastic parts which should satisfy heat resistance regulation.

• 한국지반공학회논문집
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• 제26권7호
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• pp.7-16
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• 2010

응력이력은 사질토 지반의 잔류 수평응력을 증가시켜 현장시험의 관입저항을 증가시킨다. 본 연구에서는 챔버에 조성된 정규 및 과압밀 시료에 대한 콘관입시험과 딜라토미터 시험을 수행하고 응력이력이 시험결과와 측정값의 상관관계에 미치는 영향을 분석하였다. 시험결과, 평균 유효응력으로 정규화된 콘선단저항은 상대밀도 및 상태정수와 높은 상관성을 보였다. 딜라토미터의 수평응력지수($K_D$)는 콘선단저항($q_c$) 뿐만 아니라, 딜라토미터 계수($E_D$) 보다도 응력이력 영향을 효과적으로 반영하였다. 수평응력지수에 반영된 응력이력의 영향을 정지토압계수인 $K_0$로 보정한 $K_D/K_0$는 상대밀도 뿐만 아니라, 상태정수, 평균 유효응력으로 정규화된 콘선단저항과 응력이력에 관계없는 높은 상관성을 보였다. 또한 평균 유효응력으로 정규화된 딜라토미터 계수($E_D/{\sigma}_m'$)와 상태정수의 관계도 응력이력에 관계없이 유일한 식으로 표현되었다.

• 한국지반공학회:학술대회논문집
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• 한국지반공학회 2008년도 춘계 학술발표회 초청강연 및 논문집
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• pp.628-635
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• 2008

A series of cone penetration tests in large calibration chamber were performed to investigate the effect of cementation level, relative density and vertical confining stress on cone resistance. From the experimental results, it was observed that the cone resistance is increased with increasing gypsum content, relative density, and confining stress. The increasing ratio on cone resistance of cemented sand compared with that of uncemented sand, that is IR($q_c$), was increased with increasing gypsum content and relative density, whereas it was decreased as the vertical confining stress increases. It was also observed that the cementation of granular soil influences the behavior of ground at low level of confining stress and its effect is diminished with depth.

• 한국표면공학회지
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• 제48권4호
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• pp.142-148
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• 2015

The effect of equal channel angular pressing (ECAP) on the pitting corrosion resistance of hard anodized Al5052 alloy was investigated. The degree of internal stress generated in anodic oxide films during hard anodization was also evaluated with a strain gauge method. The pitting corrosion resistance of hard anodized Al5052 alloy was greatly decreased by ECAP. Cracks occurred in the anodic oxide film during hard anodization and these cracks were larger and deeper in the alloy with ECAP than without. The pitting corrosion was accelerated by cracks. The internal stress present in the anodic oxide films was compressive and the stress was higher in the alloys with ECAP than without, resulting in an increased likelihood of cracks. The pitting corrosion resistance of hard anodized Al5052 alloy was improved by annealing at the range of 473-573K after ECAP processed at room temperature for four passes. The compressive internal stress gradually decreased with increasing annealing temperature. It is assumed that the improvement in the pitting corrosion resistance of hard anodized Al5052 alloy by annealing may be attributed to a decrease in the likelihood of cracks due to the decreased internal stresses in anodic oxide films.