• BMB Reports
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• v.42 no.1
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• pp.28-34
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• 2009

To understand the molecular mechanism underlying proline accumulation in Brassica napus, cDNAs encoding ${\Delta}^1$-pyrroline-5-carboxylate synthetase (BnP5CS), ornithine $\delta$-aminotransferase (BnOAT) and proline dehydrogenase (BnPDH) were isolated and characterized. Southern blot analysis of BnP5CSs in B. napus and its diploid ancestors suggested a gene loss may have occurred during evolution. The expression of BnP5CS1 and BnP5CS2 was induced, while the expression of BnPDH was inhibited under salt stress, ABA treatment and dehydration, prior to proline accumulation. The upregulation of BnOAT expression was only detected during prolonged severe osmotic stress. Our results indicate that stress-induced proline accumulation in B. napus results from the reciprocal action of activated biosynthesis and inhibited proline degradation. Whether the ornithine pathway is activated depends on the severity of stress. During development, proline content was high in reproductive organs and was accompanied by markedly high expression of BnP5CS and BnPDH, suggesting possible roles of proline during flower development.

• Journal of Photoscience
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• v.8 no.1
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• pp.13-17
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• 2001

Cucumber leaves subjected to light chilling stress exhibit a preferential inactivation of photosystem(PS) I relative to PSII, resulting in the photoinhibition of photosynthesis. In light chilled cucumber leaves, Cu/Zn-Superoxide dismutase(SOD) is regarded as a primary target of the light chilling stress and its inactivation is closely related to the increased production of reactive oxygen species. In the present study, we further explored that inactivation of PSI in cucumber leaves is not a light chilling specific, but general to various oxidative stresses. Oxidative stress in cucumber leaves was induced by treatment of methylviologen(MV), a producer of reactive oxygen species in chloroplasts. MV treatment decreased the maximal photosynthetic O$_2$ evolution, resulting in the photoinhibition of photosynthesis. The photoinhibition of photosynthesis was attributable to the decline in PSI functionality determined in vivo by monitoring absorption changes around 820 nm. In addition, MV treatment inactivated both antioxidant enzymes Cu-Zn-superoxide dismutase and ascorbate peroxidase known sensitive to reactive oxygen species. From these results, we suggest that chloroplast antioxidant enzymes are the primary targets of photooxidative stress, followed by subsequent inactivation of PSI.

• Korean Journal of Materials Research
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• v.28 no.7
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• pp.398-404
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• 2018

Fatigue crack growth retardation of 304 L stainless steel is studied using a neutron diffraction method. Three orthogonal strain components(crack growth, crack opening, and through-thickness direction) are measured in the vicinity of the crack tip along the crack propagation direction. The residual strain profiles (1) at the mid-thickness and (2) at the 1.5 mm away from the mid-thickness of the compact tension(CT) specimen are compared. Residual lattice strains at the 1.5 mm location are slightly higher than at the mid-thickness. The CT specimen is deformed in situ under applied loads, thereby providing evolution of the internal stress fields around the crack tip. A tensile overload results in an increased magnitude of the compressive residual stress field. In the crack growth retardation, it is found that the stresses are dispersed in the crack-wake region, where the highest compressive residual stresses are measured. Our neutron diffraction mapping results reveal that the dominant mechanism is by interrupting the transfer of stress concentration at the crack tip.

• Computers and Concrete
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• v.27 no.2
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• pp.153-162
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• 2021

This paper aims to simulate the isotropic elastic damage theory of Liu Jun (2012) using the self-programmed UMAT subroutine in the interface of ABAQUS. Liu Jun (2012)'s method based on the mechanic theory can not be used interactively with the currently commonly used finite element software ABAQUS. The advantage of this method in the paper is that it can interact with ABAQUS and provide a constitutive program framework that can be modified according to user need. The model retains the two scalar damage variables and the corresponding two energy dissipation mechanisms and damage criteria for considering the tensile and compressive asymmetry of concrete. Taking C45 concrete as an example, the relevant damage evolution parameters of its tensile and compressive constitutive model are given. The study demonstrates that the uniaxial tensile stress calculated by the subroutine is almost the same as the Chinese Concrete Design Specification (GB50010) before the peak stress, but ends soon after the peak stress. The stress-strain curve of uniaxial compression calculated by the subroutine is in good agreement with the peak stress in Chinese Concrete Design Specification (GB50010), but there is a certain deviation in the descending stage. In addition, this paper uses the newly compiled subroutine to simulate the shear bearing capacity of the shear key in a new structural system, namely the open-web sandwich slab. The results show that the damage constitutive subroutine has certain reliability.

• Proceedings of the Korean Society for Technology of Plasticity Conference
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• 2001.05a
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• pp.143-146
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• 2001

The evolution of dynamic recrystallization (DRX) was studied with torsion test for AISI 304 stainless steel in the temperature range of $900-1000^{\circ}C$ and strain rate range of 0.05-5/sec. The evolution of DRX was investigated with microstructural analysis and change of flow stress curve slope. The investigation of serrated grain boundaries using electron back scattered diffraction (EBSD) analysis indicated that the nucleated new DRX grain size was similar to the size of bulging part. Before the steady state, the dynamically recrystallizing grains do not remain a constant size and gradually grow to the size of fully DRX grain at steady state. The calculation of grain size was based on $X_{DRX}$ and the assumption, which the nucleated DRX grains are growing to the steady state, continuously. It was found that the calculated results agreed with the microstructure of the alloy.

• Proceedings of the KIEE Conference
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• 1989.11a
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• pp.159-163
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• 1989

The various degradation phenomena, such as the evolution of gases, tan $\delta$, moisture content and total acid number in power transformer insulation oil, have been measured and analyzed. Mineral oil has been degraded at laboratory by the forced thermal stress of $60^{\circ}C$ and $90^{\circ}C$ respectively. Thermal aging oil has been degraded about 17, 34, 72 days. Also, we extracted insulation oil from working transformers. We measured gases dissolved in samples, tan $\delta$, moisture content, total acid number. Activation energy and resistivity is calculated from them. It is found that gases and tan $\delta$ increases as partial discharge and total acid number increases and that conductivity of the sample increases as activation energy increases.

• Proceedings of the Korea Concrete Institute Conference
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• 2003.05a
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• pp.95-100
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• 2003

The shrinkage of high strength/high performance concrete is very important property for the good working of a structure since it very often generates early age cracking due to thermal and autogenous shrinkage. Autogenous shrinkage occurs as a result of internal moisture depletion due to hydration and temperature-induced effects. The level of autogenous shrinkage occurring due to hydration also depends on temperature history at very early age. It is necessary that effect of temperature on autogenous shrinkage is investigated since the stress generated due to autogenous shrinkage is quantified. In this study, Effect of hydration heat evolution on autogenous shrinkage of high strength concretes with W/C=25-40% was investigated.

• Proceedings of the Korean Society for Technology of Plasticity Conference
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• 2004.10a
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• pp.207-210
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• 2004

The deformation behavior of copper during equal channel angular pressing (ECAP) was calculated using a three-dimensional version of a constitutive model based on the dislocation density evolution. Finite element simulations of the variation of the dislocation density and the dislocation cell size with the number of ECAP passes are reported. The calculated stress, strain and cell size are compared with the experimental data for Cu deformed by ECAP in a modified Route C regime. The results of FEM analysis were found to be in good agreement with the experiments. After a rapid initial decrease down to about 200 nm in the first ECAP pass, the average cell size was found to change little with further passes. Similarly, the strength increased steeply after the first pass, but tended to saturate with further pressings. The FEM simulations also showed strain non-uniformities and the dependence of the resulting strength on the location within the workpiece.

• Proceedings of the KSR Conference
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• 2008.06a
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• pp.964-968
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• 2008

Infrared thermography using high-speed infrared camera has been recognized as a powerful method for various potential applications, such as nondestructive inspection, failure analysis, stress analysis, and medical fields, due to non-contact, high-speed, and high spatial resolution at various temperature ranges. In this investigation, damage evolution due to generation of hot spots on railway brake disc was investigated using the infrared thermography method. A high-speed infrared camera was used to measure the surface temperature of brake disc as well as for in-situ monitoring of hot spot evolution. From the thermographic images, the observed hot spots and thermal damage of railway brake disc during braking operation were qualitatively analyzed. Moreover, in this investigation, the previous experimental and theoretical studies on hot spots phenomenon were reviewed, and the current experimental results were introduced and compared with theoretical prediction.

• Journal of Welding and Joining
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• v.27 no.6
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• pp.49-54
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• 2009

In this study, the numerical analysis model for fatigue life prediction of welded structures are presented. In order to evaluate the structural degradation of welded structures due to fatigue loading, continuum damage mechanics approach is applied. Damage evolution equation of welded structures under arbitrary fatigue loading is constructed as a unified plasticity-damage theory. Moreover, by integration of damage evolution equation regarding to stress amplitude and number of cycles, the simplified fatigue life prediction model is derived. The proposed model is compared with fatigue test results of T-joint welded structures to obtain its validation and usefulness. It is confirmed that the predicted fatigue life of T-joint welded structures are coincided well with the fatigue test results.