• BMB Reports
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• v.28 no.6
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• pp.527-532
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• 1995

Human taurine transporter has 12 transmembrane domains and its molecular weight is 69.6 kDa. The long cytoplasmic carboxy and amino termini might function as regulatory attachment sites for other proteins. Six potential protein kinase C phosphorylation sites have been reported in human taurine transporter. In this report, we studied the effects of phorbol 12-myristate 13-acetate (PMA) and glucocorticoid hormone on taurine transportation in the RAW 264.7, mouse macrophage cell line. When the cells were incubated with $[^{3}H]taurine$ in the presence or absence of $Na^+$ ion for 40 min at $37^{\circ}C$, the [$[^{3}H]taurine$ uptake rate was 780-times higher in the $Na^{+}-containing$ buffer than in the $Na^{+}-deficient$ buffer, indicating that this cell line expresses taurine transporter protein on the cell surface. THP1, a human promonocyte cell line, also showed a similar property. The $[^{3}H]taurine$ uptake rate was not influenced by the inflammatory inducing cytokines such as interleukin-1, gamma-interferon or interleukin-1+gamma-interferon, but was decreased by the PMA in the RAW 264.7 cell line. This suggests that activation of protein kinase C inhibits taurine transporter activity directly or indirectly. The inhibition of $[^{3}H]taurine$ uptake by PMA was time-dependent. Maximal inhibition occurred in one hr stimulation with PMA Increasing the treatment time beyond one h reduced the $[^{3}H]taurine$ uptake inhibition due to the depletion or inactivation of protein kinase C. The cell line also showed concentration-dependent $[^{3}H]taurine$ uptake under PMA stimulation. The phorbol-ester caused 23% inhibition at the concentration of 1 ${\mu}m$ PMA. The inhibition was significant even at a concentration as low as 10 nM PMA The reduced $[^{3}H]taurine$ uptake could be recovered by treatment with glucocorticosteroid hormone. Dexamethasone led to recover of the reduced taurine uptake induced by phorbol-ester, recovering maximally after one hr. This may suggest that macrophage cells require higher taurine concentration in a stressed state, for the secretion of glucocorticoid hormone is increased by hypothalamo-pituitary-adrenocortical (HPA) axis activation in the blood stream.

• Journal of Korean Society for Geospatial Information Science
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• v.24 no.1
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• pp.51-59
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• 2016

Global consumption of fossil fuels continues to increase. As developing countries use fossil fuel as much as the existing fossil fuel using countries, the total amount of fossil fuel consumed has risen. The finite fossil energy depletion insecurity have become serious. In addition, fossil energy is caused by environmental pollution, economic and social problems remain in assignments that need to be addressed. Although solar power is clean and has many benefits, there are several problems in the process of installing a solar power plant. To solve these problems, floating photovoltaic plants has emerged as an alternative. This floating photovoltaic plants location analysis has not been made yet. In this study, the conditions of the floating photovoltaic plants location is analyzed with the Analytic Hierarchy Process using the terrain and climate factors. The score is assigned to the attribute information of each factor by the classification table. After multiplied by the weight the result is analyzed by visualization of the score. As the result, the score of the northen part of Gyeongsangbuk-do province is higher than the southern part of Gyeongsangbuk-do province. Especially Andongho lake in Andong City and the reservoir in Yeongyang-Gun are extracted as the optimal location. The score of the river boundary is low not the center of the river stream. It is expected that this study would be a more accurate floating solar power plant location analysis.