• Korean Journal of Veterinary Research
• /
• v.37 no.3
• /
• pp.479-487
• /
• 1997

The present study was carried out to localize the central nuclei innervating the rat colon using pseudorabies virus-Bartha strain which has been known as a very useful neurotracer. The results were as follows. The central nuclei innervating the proximal colon were premotor area, subfornical organ, preoptic area in telencephalon, and paraventricular nucleus, bed nucleus of stria terminalis, retrochiasmatic area in the diencephalon, and periaqueductal gray, Edinger-Westphal nucleus, tegmental nucleus in the mesencephalon, and parabrachial nucleus, locus ceruleus, A5 area, $K{\ddot{o}}lliker$-Fuse nucleus, magnocellular reticular nucleus in the metencephalon, and nucleus tractus solitarius, A1 noradrenergic cell group, dorsal motor nucleus of vagus nerve, nucleus ambiguus, area postrema in the myelencephalon. In the spinal cord, the thoracic division had some nuclei innervating the proximal colon. The nuclei innervating the distal colon were paraventricular nucleus of the diencephalon, Edinger-Westphal nucleus of midbrain, and parabrachial nucleus, locus ceruleus, A5 area, $K{\ddot{o}}lliker$-Fuse nucleus, magnocellular reticular nucleus of the metencephalon, and nucleus tractus solitarius, dorsal motor nucleus of vagus nerve, nucleus ambiguus, area postrema in the myelencephalon. In the spinal cord, thoracic, lumbar and sacral division innervated the distal colon.

• Geotechnical Engineering
• /
• v.13 no.4
• /
• pp.149-162
• /
• 1997

A series of torsion shear tests were performed to study the strength characteristics of sand under various stress paths during rotation of principal stress. These results can be classified into two groups of 25cm and 40cm according to the height of specimen, and toy que was applied only in the clockwise direction. In this study, strength characteristics of sand for the principal stress ratio in torsion sheartests were investigated and their results were compared with Lade's failure criterion. And the effect for specimen was considered. From the results of tests, friction angle of sand was affected by the deviatoric principal stress ratio $b:(\sigma_2 -\sigma_s)/(\sigma_2, -\sigma_3)$Failure strength of sand was determined not by the stress paths but by the current stress state. From comparison of specimens on 25cm and 40cm height, effect of end restraint could not be found. In the test where b is over 0.5 due to extension force, necking phenomenon by the strain localization was found.

• Journal of Microbiology and Biotechnology
• /
• v.20 no.12
• /
• pp.1630-1636
• /
• 2010

During the fermentation process of Saccharomyces cerevisiae, yeast cells must rapidly respond to a wide variety of external stresses in order to survive the constantly changing environment, including ethanol stress. The accumulation of ethanol can severely inhibit cell growth activity and productivity. Thus, the response to changing ethanol concentrations is one of the most important stress reactions in S. cerevisiae and worthy of thorough investigation. Therefore, this study examined the relationship between ethanol tolerance in S. cerevisiae and a unique protein called alcohol sensitive RING/PHD finger 1 protein (Asr1p). A real-time PCR showed that upon exposure to 8% ethanol, the expression of Asr1 was continuously enhanced, reaching a peak 2 h after stimulation. This result was confirmed by monitoring the fluorescence levels using a strain with a green fluorescent protein tagged to the C-terminal of Asr1p. The fluorescent microscopy also revealed a change in the subcellular localization before and after stimulation. Furthermore, the disruption of the Asr1 gene resulted in hypersensitivity on the medium containing ethanol, when compared with the wild-type strain. Thus, when taken together, the present results suggest that Asr1 is involved in the response to ethanol stress in the yeast S. cerevisiae.

• Journal of Microbiology and Biotechnology
• /
• v.6 no.5
• /
• pp.321-325
• /
• 1996

Mutants having altered levels and/or types of EPS in exopolysaccharide biosynthesis were isolated after NTG mutagenesis of Zoogloea ramigera 115SLR. Mutant candidates were classfied with five groups based on the observed characteristics for EPS biosynthesis pattern. The recombinant plasmids pLEX3BS and pLEX3BM were constructed from pEX3B which was previously isolated from genomic DNA of Z. ramigera 115SLR. Plasmid pLEX3BM with a 7.8 kb insert DNA contains an additional 1.8kb DNA fragment which is not present in pLEX3BS containing 13 kb insert DNA. Plasmid pLEX3BM was able to complement the mutation responsible for the changes in morphology of Z. ramigera 115SLR. However, the complementation of EPS negative mutant strains was not successful with pLEX3BM. Plasmid pLEX3BS on the other hand complemented the mutation responsible for the loss of EPS biosynthesis, resulting in the restoration of Z. ramigera 115SLR phenotype. But this plasmid was not able to complement the morphological mutant strain, Z. ramigera 115SLR.

• Journal of the Korean Society of Manufacturing Process Engineers
• /
• v.19 no.9
• /
• pp.78-84
• /
• 2020

This paper introduces the structural and vibration analysis performed in the localization development process of hydraulic pumps used in wheeled armored vehicles. The maximum strain, maximum stress, maximum displacement, and minimum safety factor were calculated using structural analysis. Furthermore, it was found that the dangerous resonance frequency was avoided through vibration analysis. In addition, the reliability of the analysis results was verified by passing various tests, such as the actual vibration test and the actual durability test. The developed hydraulic pump is expected to contribute significantly to the maintenance of military vehicles in the future.

• Structural Engineering and Mechanics
• /
• v.65 no.3
• /
• pp.223-231
• /
• 2018

A numerical investigation of the impact of steel ductility on the strength and ductility of two-way corner and edge-supported concrete slabs containing low ductility welded wire fabric is presented. A finite element model was developed for the investigation and the results of a series of concurrent laboratory experiments were used to validate the numerical solution. A parametric investigation was conducted using the numerical model to investigate the various factors that influence the structural behavior at the strength limit state. Different values of steel uniform elongation and ultimate to yield strength ratios were considered. The results are presented and evaluated, with emphasis on the strength, ductility, and failure mode of the slabs. It was found that the ductility of the flexural reinforcement has a significant impact on the ultimate load behavior of two-way corner-supported slabs, particularly when the reinforcement was in the form of cold drawn welded wire fabric. However, the impact of the low ductility WWF has showed to be less prominent in structural slabs with higher levels of structural indeterminacy. The load-deflection curves of corner-supported slabs containing low ductility WWF are brittle, and the slabs have little ability to undergo plastic deformation at peak load.

• Structural Engineering and Mechanics
• /
• v.55 no.2
• /
• pp.315-334
• /
• 2015

This study's primary aim is to check the existence of a representative volume element for granular materials and determine the link between the properties (responses) of macro structures and the size of the discrete particle assembly used to represent a constitutive relation in a two-scale model. In our two-scale method the boundary value problem on the macro level was solved using finite element method, based on the Cosserat continuum; the macro stresses and modulus were obtained using a solution of discrete particle assemblies at certain element integration points. Meanwhile, discrete particle assemblies were solved using discrete element method under boundary conditions provided by the macro deformation. Our investigations focused largely on the size effects of the discrete particle assembly and the radius of the particle on macro properties, such as deformation stiffness, bearing capacity and the residual strength of the granular structure. According to the numerical results, we suggest fitting formulas linking the values of different macro properties (responses) and size of discrete particle assemblies. In addition, this study also concerns the configuration and displacement fluctuation of discrete particle assemblies on the micro level, accompanied with the evolution of bearing capacity and deformation on the macro level.

• Nuclear Engineering and Technology
• /
• v.33 no.1
• /
• pp.93-101
• /
• 2001

The spacer grid is one of the main structural components in the fuel assembly, which supports the fuel rods, guides cooling water, and protects the system from an external impact load, such as earthquakes. Therefore, the mechanical and structural properties of the spacer grids must be extensively examined while designing them. In this paper, a numerical method for predicting the buckling strength of spacer grids is presented. Numerical analyses on the buckling behavior of the spacer grids are performed for a various array of sizes of the grids considering that the spacer grid is an assembled structure with thin-walled plates and imposing proper boundary conditions by nonlinear dynamic finite element method using ABAQUS/Explicit. Buckling tests on several numbers of specimens of the spacer grid were also carried out in order to compare the results between the test and the simulation result. The drop test is accomplished by dropping a carriage on the specimen at a pre-determined position. From this test, the specimens are buckled only at the uppermost and the lowermost layer among the multi-cells, which is similar to the local buckling at the weakest point of the grid structure. The simulated results also similarly predicted the local buckling phenomena and were found to give good correspondence with the experimental values for the thin-walled grid structures.

• Journal of the Korean Society of Propulsion Engineers
• /
• v.4 no.1
• /
• pp.1-12
• /
• 2000

This study summarizes the numerical analyses of a Ti alloy deformation under a back extrusion process. Amongst metallic parts in a small propulsion motor case, a Ti-6Al-4V alloy is used extensively. However, the Ti alloy shows a great deal of shear band formation which often leads to a fracture due to a narrow working temperature window. Moreover, the shear band tends to develop over an area where a contact occurs between the hot work piece and the die wall, due to localized cooling. Thus, heating the dies is often required to overcome the deformation localization. Therefore, it becomes necessary to investigate the internal temperature and strain rate distribution during forging process of a Ti alloy. Furthermore, a shear band analysis is peformed using a finite difference scheme and a comparison is made between steel and Ti alloy.

• Journal of Microbiology and Biotechnology
• /
• v.27 no.9
• /
• pp.1664-1669
• /
• 2017

Gamma-aminobutyric acid is a precursor of nylon-4, which is a promising heat-resistant biopolymer. GABA can be produced from the decarboxylation of glutamate by glutamate decarboxylase. In this study, a synthetic scaffold complex strategy was employed involving the Neurospora crassa glutamate decarboxylase (GadB) and Escherichia coli GABA antiporter (GadC) to improve GABA production. To construct the complex, the SH3 domain was attached to the N. crassa GadB, and the SH3 ligand was attached to the N-terminus, middle, and C-terminus of E. coli GadC. In the C-terminus model, 5.8 g/l of GABA concentration was obtained from 10 g/l glutamate. When a competing pathway engineered strain was used, the final GABA concentration was further increased to 5.94 g/l, which corresponds to 97.5% of GABA yield. With the introduction of the scaffold complex, the GABA productivity increased by 2.9 folds during the initial culture period.