• Mycobiology
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• v.44 no.3
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• pp.171-179
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• 2016

In the screening of marine mangrove derived fungi for lovastatin productivity, endophytic Aspergillus luchuensis MERV10 exhibited the highest lovastatin productivity (9.5 mg/gds) in solid state fermentation (SSF) using rice bran. Aspergillus luchuensis MERV10 was used as the parental strain in which to induce genetic variabilities after application of different mixtures as well as doses of mutagens followed by three successive rounds of genome shuffling. Four potent mutants, UN6, UN28, NE11, and NE23, with lovastatin productivity equal to 2.0-, 2.11-, 1.95-, and 2.11-fold higher than the parental strain, respectively, were applied for three rounds of genome shuffling as the initial mutants. Four hereditarily stable recombinants (F3/3, F3/7, F3/9, and F3/13) were obtained with lovastatin productivity equal to 50.8, 57.0, 49.7, and 51.0 mg/gds, respectively. Recombinant strain F3/7 yielded 57.0 mg/gds of lovastatin, which is 6-fold and 2.85-fold higher, respectively, than the initial parental strain and the highest mutants UN28 and NE23. It was therefore selected for the optimization of lovastatin production through improvement of SSF parameters. Lovastatin productivity was increased 32-fold through strain improvement methods, including mutations and three successive rounds of genome shuffling followed by optimizing SSF factors.

• Transactions of Materials Processing
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• v.24 no.5
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• pp.348-353
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• 2015

The PEPS(Polar-coordinated Effective Plastic Strain) FLD(Forming Limit Diagram), a new type of FLD based on a polar representation of the EPS(Effective Plastic Strain), appears to be an effective solution to the problem of non-linear strain path effects. This method has the advantages of the familiar strain-based diagram for linear loading, but without the strain-hardening limitations of the stress-based diagram, or non-intuitive aspects of alternate Cartesian diagrams based on effective plastic strain. In the current study, the PEPS FLD was applied to the development process of an aluminum automobile-body panel, including the necking or crack prediction, die design, and die modification. As a result, the PEPS FLD provided improved formability of aluminum sheet as compared to deriving the potential formability with non-linearity.

• Proceedings of the Korean Geotechical Society Conference
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• 2008.03a
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• pp.170-180
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• 2008

Successful design, construction and maintenance of NATM tunnel demands prediction, control, stability guidelines, the estimation pre-improvement support system and monitoring of surface settlement, gradient and ground displacement with high accuracy. Moreover, urban NATM tunnel under difficult geotechnical conditions is important the estimation and necessary of pre-improvement support system. Various strategies have been proposed for the quantitative estimation of pre-improvement support system. This paper was investigated and analysed an assessment technique for the quantitative estimation of pre-improvement support system on underground space, as mountain and urban tunnel, in detail. The analysis performed on design and construction stage with field database using the proposed stability estimation index by many researcher including the critical strain and the apparent Young's modulus concept.

• Korean Journal of Agricultural Science
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• v.37 no.3
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• pp.491-499
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• 2010

This study was carried out to investigate the consolidation characteristics of the remolded clay by the oedometer and the constant rate of strain(CRS) consolidation tests. As the rate of strain increases, the settlement rapidly decreased. As the ratio of the sand in the specimen increases, its effect on the rate of strain to the settlement was reduced. As the effective stress increased, the void ratio decreased, while the rate of strain increased, it did not show a clear variation. The reduction of the void ratio was shown to be less than the oedometer test. The coefficient of vertical consolidation with effective stress showed very large variation around preconsolidation stress, but the rate of strain did not provide significant effects. The rate of strain with effective stress gradually decreased at all tests and mixed ratio of sand. The rate of strain at the constant rate of strain tests showed smaller than in the oedometer test. The coefficient of consolidation at the constant rate of strain tests showed much more increase than in the oedometer test. The ratio of the vertical coefficient of consolidation by the odometer and the constant rate of strain tests showed a large difference according to various tests method and mixing ratio. Therefore, it is recommended that careful attention should be paid to designing the soft ground improvement.

• Journal of Korea Technical Association of The Pulp and Paper Industry
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• v.39 no.3
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• pp.24-29
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• 2007

The stress and strain properties of KOCC were modified to improve the performance of KOCC as a packaging raw material. The refining consistency, refining degree, blending conditions and the grammage of handsheet were varied. The stress-strain properties, tensile energy absorption were measured. The refining improved significantly the stress and strain properties of paper, especially at lower refining consistency. The increase of grammage also contributed to the improvement in the stress and strain of paper. It was also found that the refining, blending and grammage contributed to the increase of tensile energy absorption. However, it is strongly recommended to apply the combination of refining consistency, refining degree and mechanical treatment(blending).

• International Journal of Concrete Structures and Materials
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• v.10 no.1
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• pp.75-85
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• 2016

Fire ranks high among the potential risks faced by most buildings and structures. A full understanding of temperature effects on fiber reinforced concrete is still lacking. This investigation focuses on the study of the residual compressive strength, stress strain behavior and surface cracking of structural polypropylene fiber-reinforced concrete subjected to temperatures up to $300^{\circ}C$. A total of 48 cubes was cast with different fiber dosages and tested under compression after exposing to different temperatures. Concrete cubes with varying macro (structural) fiber dosages were exposed to different temperatures and tested to observe the stress-strain behavior. Digital image correlation, an advanced non-contacting method was used for measuring the strain. Trends in the relative residual strengths with respect to different fiber dosages indicate an improvement up to 15 % in the ultimate compressive strengths at all exposure temperatures. The stress-strain curves show an improvement in post peak behavior with increasing fiber dosage at all exposure temperatures considered in this study.

• Journal of Microbiology and Biotechnology
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• v.11 no.2
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• pp.222-228
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• 2001

Vibrio metschnikovii strain RH530 is a non-pathogenic, industrially-important alkaline protease producer which has been isolated from wastewater. In this paper, we report on the transformation of this strain by using the method of electroporation. A field strength of $7.5\;kVcm^{-1}$ and $25\;{\mu}F$, and using a 0.2-cm cuvette, appeared to be the optimal conditions for electroporation of the cells with the recombinant pSBCm plasmid carrying the vapK alkaline protease gene and the ColE1 replicon. Cells were subjected to osmotic shock in order to remove extracelluar DNase, and adding 200 mM of sucrose to electroporation buffer cells showed an increased transformation efficiency. Maximum efficiency of transformation was obtained at an early exponential growth phase. Using all of the conditions mentioned above, we routinely obtained a transformation efficiency of more than $10^4{({\mu}g\;plasmid\;DNA)}^{-1}$. The stability of the plasmid pSBCm in V. metschnikovii RH530 was 25% after 18h of growth (27 generations) in the medium without antibiotic selection. The insertion of the par locus to the pSBCm increased the stability of the plasmid up to 42% without selective pressure. The increase in plasmid stability was accompanied by the increase in the productivity of alkaline protease in the recombinant V. metschnikovii strain RH530. Determining optimal conditions for the transformation of the industrially-important, nonpathogenic Vibrio strain, and the improvement of plasmid stability by introducing the par locus into the high copy number plasmid vector, will allow the development of procedures involved in the genetic manipulation of this strain, particularly for its use in the production of industrial enzymes such as alkaline protease.

• Biomolecules & Therapeutics
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• v.1 no.1
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• pp.26-30
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• 1993

Strain of treptomyces minoensis DMCJ-144 was tried to be improved so that it produces much more the $\alpha$-amylase inhibitor. Streptomyces minoensis DMCJ-144 was treated with 1 mg/mι (pH 9.0) of N-methyl-N'-nitro-N-nitrosoguanidine at $30^{\circ}C$ for 60 min and irradiated with UV light distanced 30 cm for 20 min. After mutagenesis, surviving colonies were cultured on the CM contaning acriflavine ($10{\mu}g/ml$) three times in order to enhance the mutability. And then through multi-level screening, colonies that ${\alpha}$-amylase inhibitor productibility. was Improved were selected by modified-blue value method. After third acriflavine treatment, $\alpha$-amylase inhibitory activities of selected colonies were found to be much better as compared with that of parent strain. One mutant strain showed 5.4 time inhibitory activity than the parent strain.

• Proceedings of the Korean Society for Technology of Plasticity Conference
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• 2006.06a
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• pp.34-39
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• 2006

An automated surface-strain measurement system, named ASIAS, was developed by using the image processing and stereo vision techniques in the previous studies by the corresponding author and his coworkers. This system has been upgraded mainly to improve the accuracy through image enhancement, sub-pixel measurement, surface smoothing, etc., since the first version was released. The present study has still more improved the convenience of users as well as the accuracy of measurement by processing high resolution images 8 mega pixels or more which can be easily obtained from a portable digital steal camera. It is proved that high resolution image processing greatly decreases the measurement error and gives strain data without considerable deterioration of accuracy even when the deformed grids to be measured and the master grids for camera calibration are captured together in the same image, making the whole process of strain measurement much simpler.

• Proceedings of the Korea Technical Association of the Pulp and Paper Industry Conference
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• 2006.06b
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• pp.445-450
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• 2006

The stress and strain properties of KOCC were modified to improve the performance of KOCC as a packaging raw material. The refining consistency, refining degree and the grammage of handsheet were varied. The stress strain properties, tensile energy absorption were measured for prepared handsheets. The refining improved significantly the stress and strain properties of paper, especially at lower refining consistency. The increase of grammage also had contributed to improve the stress and strain of paper. It was also found that both refining and grammage contributed to the improvement of tensile energy absorption. However, it is strongly recommended to introduce the combination of refining consistency, refining degree and mechanical treatment.