• 한국균학회소식:학술대회논문집
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• 2014.05a
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• pp.39-39
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• 2014

In a homothallic filamentous fungus Aspergillus nidulans, sexual and asexual developments are largely affected by the genetic and environmental factors. To regulate the complex subsets of genes involved in the developmental processes accurately, tight regulations of transcription factors are required. The forkhead type transcription factors are the class of regulators that function in a broad spectrum of cellular and developmental processes in many species from yeast to human. Here, we identified the fkhA and fkhB genes that encode a conserved forkhead transcription factors. The fkhA deletion resulted in the complete loss of fruiting body formation under all conditions favoring sexual development, suggesting that the fkhA gene is required for sexual development in A. nidulans. Overexpression of fkhA resulted in enhanced formation of fruiting bodies under induction condition not only in the normal condition but also in the condition of presence of 0.6 M KCl, which strongly inhibits sexual development. To know the function of the fkhB gene, we also generated fkhB knock-out strain in A. nidulans. Deletion of fkhB resulted in abnormal conidiophore formation under standard conditions and delayed sexual development process, suggesting that the fkhB gene plays an important role in conidiophore morphogenesis Taken together, these results suggest that the fkhA gene is necessary and sufficient for regulating sexual development and the fkhB gene is a transcription factor related in asexual developmental process in A. nidulans.

• Korean Journal of Environmental Agriculture
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• v.25 no.3
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• pp.228-235
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• 2006

Pseudomonas aeruginosa $GRC_2$, siderophore-producing strain, inhibited growth of Macrophomina phaseolina in vitro and reduced charcoal rot in seeds of Brassica juncea in field when coated with adaptive strains. P. aeruginosa $GRC_2$ and Azotobacter chroococcum $AC_1$ produced indole-3-acetic acid and solubilized insoluble phosphate. A. chroococcum $AC_1$ fixed nitrogen asymbiotically. Urea and diammonium phosphateadaptive variant strains of P. aeruginosa and A. chroococcum strongly inhibited M. phaseolina in comparison to parental strains. Bacterization of seeds induced seed germination, seedling growth, and enhanced yield of B. juncea by 10.87% as compared to full doses of urea and diammonium phosphate. Both adaptive strains of chemical fertilizers aggressively colonized roots, showing effectiveness to growth and developments of B. juncea.

• Journal of The Korean Society of Agricultural Engineers
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• v.59 no.5
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• pp.101-108
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• 2017

Recently, the occurrence of landslides has been increasing over the years due to the extreme weather event. Developments of landslides monitoring technology that reduce damage caused by landslide are urgently needed. Therefore, in this study, a strain ratio sensor was developed to predict the ground behavior during the slope failure, and the change in surface ground displacement was observed as slope failed on the field model experiment. As a result, in the slope failure, the ground displacement process increases the risk of collapse as the inverse displacement approaches zero. It is closely related to the prediction of precursor. In all cases, increase in displacement and reverse speed of inverse displacement with time was observed during the slope failure, and it is very important event for monitoring collapse phenomenon of risky slopes. In the future, it can be used as disaster prevention technology to contribute in reduction of landslide damage and activation of measurement industry.

• Proceedings of the Microbiological Society of Korea Conference
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• 2000.05a
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• pp.58-65
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• 2000

Diarrheal diseases are a major cause of both illness and death in developing countries and are caused by rotavirus, Shigella spp., Salmonella spp., enterotoxigenic Escherichia coli (ETEC), and Vibrio spp. In this study, for the development of vaccine against diarrheal diseases caused by Shigella sonei, Salmonella typhimurium, E. coli O157, and Vibrio cholerae, cloning and nucleotide sequence analysis of genes and characteristics of their gene products in E. coli were performed. For construction of attenuated strain of S. sonnei KNIH104 and Salmonella typhimurium KNIH100, the aroA genes were cloned, respectively. The recombinant plasmid $_pJP{\Delta}A45$ containing aroA deleted region and suicide vector $(_pJP5603)$ was constructed. The aroA gene deleted mutants were constructed using this recombinant plasmid. For cloning gene encoding antigenic region of E. coli O157 KNIH317, the O-antigen synthesis gene cluster and sit gene was cloned. The E. coli XL1-Blue cells harboring this recombinant plasmid showed cytotoxicity in Vero cells. The ctx gene was cloned for tile purpose of antigenic region against V. cholerae KNIH002. Sequence analysis confirmed that the virulence gene cassette was consisted of ace, zot, ctxA and ctxB genes.

• Steel and Composite Structures
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• v.32 no.4
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• pp.443-454
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• 2019

With the aim to put forward the analytical model for calculating the shear capacity of precast steel reinforced concrete (PSRC) beams, a static test on two full-scale PSRC specimens was conducted under four-point loading, and the failure modes and strain developments of the specimens were critically investigated. Based on the test results, a modified truss-arch model was proposed to analyze the shear mechanisms of PSRC and cast-in-place SRC beams. In the proposed model, the overall shear capacity of PSRC and cast-in-place SRC beams can be obtained by combining the shear capacity of encased steel shape with web concrete determined by modified Nakamura and Narita model and the shear capacity of reinforced concrete part determined by compatible truss-arch model which can consider both the contributions of concrete and stirrups to shear capacity in the truss action as well as the contribution of arch action through compatibility of deformation. Finally, the proposed model is compared with other models from JGJ 138 and AISC 360 using the available SRC beam test data consisting of 75 shear-critical PSRC and SRC beams. The results indicate that the proposed model can improve the accuracy of shear capacity predictions for shear-critical PSRC and cast-in-place SRC beams, and relatively conservative results can be obtained by the models from JGJ 138 and AISC 360.

• Structural Engineering and Mechanics
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• v.84 no.1
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• pp.63-76
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• 2022

Major engineering requirements and technological developments in the steel construction industry are discussed to support a new innovative system, namely corrugated web beams, for future structural projections. These new-generation steel beams, fabricated as welded plate girders with corrugated webs, are designed to combine large spans with very low weight. In the present study, the flexural capacity of optimally designed trapezoidal and sinusoidal corrugated web beams was aimed at. For this purpose, the new metaheuristic methods, specifically hunting search and firefly algorithms, were used for the minimum weight design of both beams according to the rules of Eurocode EN 1193 15 and DASt-Ri 015. In addition, the strengthening effects of the corrugation geometry at the web posts on the load capacity of fabricated steel beams were tested in a reaction frame. The experimental tests displayed that the lateral capacity of trapezoidal web beams is more durable under flexural loads compared to sinusoidal web beams. These thin-walled beams were also simulated using a 3-D finite element model with plane strain to validate test results and describe the effectiveness of the ABAQUS software.

• Proceedings of the Korean Society for Applied Microbiology Conference
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• 2004.06a
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• pp.60-61
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• 2004

Metabolic engineering is now a well established discipline, used extensively to determine and execute rational strategies of strain development to improve the performance of micro-organisms employed in industrial fermentations. The basic principle of this approach is that performance of the microbial catalyst should be adequately characterised metabolically so as to clearlyidentify the metabolic network constraints, thereby identifying the most probable targets for genetic engineering and the extent to which improvements can be realistically achieved. In order to harness correctly this potential, it is clear that the physiological analysis of each strain studied needs to be undertaken under conditions as close as possible to the physico-chemical environment in which the strain evolves within the full-scale process. Furthermore, this analysis needs to be undertaken throughoutthe entire fermentation so as to take into account the changing environment in an essentially dynamic situation in which metabolic stress is accentuated by the microbial activity itself, leading to increasingly important stress response at a metabolic level. All too often these industrial fermentation constraints are overlooked, leading to identification of targets whose validity within the industrial context is at best limited. Thus the conceptual error is linked to experimental design rather than inadequate methodology. New tools are becoming available which open up new possibilities in metabolic engineering and the characterisation of complex metabolic networks. Traditionally metabolic analysis was targeted towards pre-identified genes and their corresponding enzymatic activities within pre-selected metabolic pathways. Those pathways not included at the onset were intrinsically removed from the network giving a fundamentally localised vision of pathway functionality. New tools from genome research extend this reductive approach so as to include the global characteristics of a given biological model which can now be seen as an integrated functional unit rather than a specific sub-group of biochemical reactions, thereby facilitating the resolution of complexnetworks whose exact composition cannot be estimated at the onset. This global overview of whole cell physiology enables new targets to be identified which would classically not have been suspected previously. Of course, as with all powerful analytical tools, post-genomic technology must be used carefully so as to avoid expensive errors. This is not always the case and the data obtained need to be examined carefully to avoid embarking on the study of artefacts due to poor understanding of cell biology. These basic developments and the underlying concepts will be illustrated with examples from the author's laboratory concerning the industrial production of commodity chemicals using a number of industrially important bacteria. The different levels of possibleinvestigation and the extent to which the data can be extrapolated will be highlighted together with the extent to which realistic yield targets can be attained. Genetic engineering strategies and the performance of the resulting strains will be examined within the context of the prevailing experimental conditions encountered in the industrial fermentor. Examples used will include the production of amino acids, vitamins and polysaccharides. In each case metabolic constraints can be identified and the extent to which performance can be enhanced predicted

• The Plant Pathology Journal
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• v.33 no.2
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• pp.193-205
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• 2017

Histone methylation plays important roles in regulating chromatin dynamics and transcription in eukaryotes. Implication of histone modifications in fungal pathogenesis is, however, beginning to emerge. Here, we report identification and functional analysis of a putative JmjC-domain-containing histone demethylase in Magnaporthe oryzae. Through bioinformatics analysis, we identified seven genes, which encode putative histone demethylases containing JmjC domain. Deletion of one gene, MoJMJ1, belonging to JARID group, resulted in defects in vegetative growth, asexual reproduction, appressorium formation as well as invasive growth in the fungus. Western blot analysis showed that global H3K4me3 level increased in the deletion mutant, compared to wild-type strain, indicating histone demethylase activity of MoJMJ1. Introduction of MoJMJ1 gene into ${\Delta}Mojmj1$ restored defects in pre-penetration developments including appressorium formation, indicating the importance of histone demethylation through MoJMJ1 during infection-specific morphogenesis. However, defects in penetration and invasive growth were not complemented. We discuss such incomplete complementation in detail here. Our work on MoJMJ1 provides insights into H3K4me3-mediated regulation of infection-specific development in the plant pathogenic fungus.

• Computers and Concrete
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• v.7 no.4
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• pp.347-364
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• 2010

Since its invention in the $19^{th}$ century, Reinforced Concrete (RC) has been widely used in the construction of a lot of different structures, as buildings, bridges, nuclear central plants, or even ships. The details of the mechanical response for this kind of structures depends directly upon the material behavior of each component: concrete and steel, as well as their interaction through the bond-slip, which makes a rigorous engineering analysis of RC structures quite complicated. Consequently, the practical calculation of RC structures is done by adopting a lot of simplifications and hypotheses validated in the elastic range. Nevertheless, as soon as any RC structural element is working in the inelastic range, it is possible to obtain the numerical prediction of its realistic behavior only through the use of non linear analysis. The aim of this work is to develop a new kind of Finite Element: the "Enhanced Solid Element (ESE)" which takes into account the complex composition of reinforced concrete, being able to handle each dissipative material behavior and their different deformations, and on the other hand, conserving a simplified shape for engineering applications. Based on the recent XFEM developments, we introduce the concept of nodal enrichment to represent kinematics of steel rebars as well as bonding. This enrichment allows to reproduce the strain incompatibility between concrete and steel that occurs because of the bond degradation and slip. This formulation was tested with a couple of simple examples and compared to the results obtained from other standard formulations.

• Journal of Korean Tunnelling and Underground Space Association
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• v.18 no.6
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• pp.535-544
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• 2016

Special tunnel design and construction methods have been suggested due to developments of subway and tunnel. Collapse accidents of tunnel bring enormous damage. So, observation and analysis for the safety of tunnelling and behaviour of surrounding ground are important. But, it is not economical to implement the field test in every time. Therefore, this study has measured ground behaviour due to excavation of 2-arch tunnel station according to offset between grouped pile and tunnel by laboratory model test. For the model test, trapdoor device was adopted. Tunnelling is simulated by volume loss of 2-arch tunnel. Ground displacements are observed by close range photogrammetric method and image processing. In addition, these data are compared with numerical analysis.