• Korean Journal of Plant Resources
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• v.29 no.1
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• pp.39-45
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• 2016

Pink Rot on melon and White Stain Symptom on grape are caused by Trichothecium roseum, one of the most important diseases of grape and melon. These diseases have been occurred in national-wide in Korea and causes irreversible damage on the grape and the melon at harvest season. This research presents the evaluation of the capacity of Bacillus subtillis HK2 to protect both melon and grape against T. reseum and establishes its role as a biocontrol agent. In this study, we isolated a Bacillus strain HK2 from rhizosphere soil, identified it as Bacillus subtillis by 16S rRNA analysis and demonstrated its antifungal activity against T. roseum. Under I-plate assay it was observed that the effect of hyphal growth inhibition was not due to production of volatile compounds. The optimum culture condition of HK2 was found at 30℃ and initial pH of 7.0. Application of HK2 culture suspension reduced 90.2% of white stain symptom on grape as compared to control, resulting in greater protection to grape against T. roseum infestation. Butanol extract of HK2 culture purified using flash column chromatography. The antifungal material was a polar substance as it showed antifungal activity in polar elute. Therefore, our results indicated a clear potential of B. subtilis HK2 to be used for biocontrol of Pink rot in melon and white stain symptom on grape caused by T. roseum.

• Korean Journal of Food Science and Technology
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• v.37 no.6
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• pp.1005-1011
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• 2005

Widespread distributions of repetitive DNA elements in bacteria genomes are useful for analysis of genomes and should be exploited to differentiate food-borne pathogenic bacteria among and within species. Enterobacterial repetitive intergenic consensus (ERIC) sequence has been used for ERIC-PCR genomic fingerprinting to identify and differentiate bacterial strains from various environmental sources. ERIC-PCH genomic fingerprinting was applied to detect and differentiate four major Gram-negative food-borne bacterial pathogens, Esherichia coli, Salmonella, Shigella, and Vibrio. Target DNA fragments of pathogens were amplified by ERIC-PCR reactions. Dendrograms of subsequent PCR fingerprinting patterns for each strain were constructed, through which relative similarity coefficients or genetic distances between different strains were obtained numerically. Numerical comparisons revealed ERIC-PCR genotyping is effective for differentiation of strains among and within species of food-borne bacterial pathogens, showing ERIC-PCR fingerprinting methods can be utilized to differentiate isolates from outbreak and to determine their clonal relationships among outbreaks.

• Microbiology and Biotechnology Letters
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• v.38 no.4
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• pp.379-384
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• 2010

We isolated microorganisms presenting high enzymatic activities for amylase, cellulase, protease, lipase or fibrinolysis from Korean traditional soybean sauce and paste. Then, the physiological properties and 16S rRNA sequences of isolated microorganisms were analyzed. All of the isolated 13 strains possessing high extra cellular enzyme activities have higher amylase and cellulase activities than Bacillus subtilis KACC 10114. All the selected strains have protease activities except for D2-14. Except D8-8 and K4-1, other strains have lipase activity. D2-7, D8-8 and K4-1 strains have higher fibrinolytic activities than others, while D8-2 strain has no activity. Most of the selected strains showed antibacterial activity even in gram positive and gram negative bacteria and yeast. Gene sequence analysis of 16S rRNA from isolated strains revealed that all the selected strains were member of Bacillus species.

• Microbiology and Biotechnology Letters
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• v.42 no.4
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• pp.393-401
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• 2014

A bacterium producing antimicrobial substance was isolated from cheonggukjang. The bacterium was identified as a strain of Bacillus subtilis by 16S rDNA sequencing and designated as Bacillus subtilis HH28. The antimicrobial substance produced from Bacillus subtilis HH28 was purified by 0-80% ammonium sulfate precipitation, DEAE-sepharose FF column chromatography, and Sephacryl S-200 HR gel chromatography. The molecular weight of the purified antimicrobial substance was estimated to be approximately 3,500 Da using Tricine sodium dodecyl sulfate-polyacrylamide gel electrophoresis and direct detection analysis. Antimicrobial substance from B. subtilis HH28 not only inhibited B. cereus, but also Listeria monocytogenes and Vibrio parahaemolyticus. The purified antimicrobial substance was stable at $40-80^{\circ}C$, and between pH 2 and 8. Antimicrobial activity of the purified substance was completely destroyed by treatment of protease, proteinase K, and pronase E, indicating that it is proteinaceous.

• Journal of the Korea institute for structural maintenance and inspection
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• v.19 no.4
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• pp.116-124
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• 2015

The objective of this study is to evaluate the effects of the replacement levels of grinded fly-ash on the repaired mortar based on a polymer. The main parameters are the curing temperature and replacement levels of grinded fly-ash. The curing temperature and the replacement levels of grinded fly-ash are varied at $40^{\circ}C$, $20^{\circ}C$ and $5^{\circ}C$, and between 0% and 35% of the total binder by weight, respectively. The flow in fresh mortar and compressive strengths according to ages, the relationship of stress-strain, elastic modulus and modulus rupture in hardened mortar, as well as scanning the electron microscopy and the X-ray diffraction of mortar, were measured, respectively. The test results showed that the flow, elastic modulus and modulus rupture are great in mortar specimens with 20~30% of the replacement levels of grinded fly-ash. In addition, compressive strengths according to ages were affected by the replacement levels of grinded fly-ash and the curing temperature indicated that the strength development ratio of mortar with 20% of the replacement levels of grinded fly-ash was greater than others. In the prediction of the compressive strength specified by the ACI 209 code, the strength development at an early and late age can be generalized by the functions of the replacement levels of grinded fly-ash and the curing temperature. In the analysis of scanning the electron microscopy and the X-ray diffraction, the number and intensity of peaks increased and the form of CSH gels on the surface of the particle of grinded fly-ash was observed.

• Journal of the Korean Society of Food Science and Nutrition
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• v.40 no.3
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• pp.466-469
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• 2011

The purpose of this study was to obtain the fatty acid composition and the antibacterial activity against pathogen of acne in various parts including seed, flesh and fruit from ripened black raspberry. In fatty acid analysis of lyophilized black raspberry, total fat of dried seed (11.047%) was much higher than in other parts. Moreover, linoleic acid (5.577%) and linolenic acid (2.317%) identified as the major fatty acids in the seed. By contrast, highest fatty acid of dried flesh was palmitic acid (0.323%) but it was remarkably low level compared with fatty acid composition of dried seed. Total polyphenol content in seed extract and flesh extract were 123.64 mg/g and 55.43 mg/g, respectively. DPPH radical scavenging ability of seed extract was higher than flesh and fruit extract. In antibacterial activity experiment against strains related acne such as Propionibacterium acne and Staphylococcus epidermidis, antibacterial activity of flesh extract was stronger than seed extract. However, seed extract showed antibacterial activity of 76.96~89.08% against each strain compared to activity of flesh extract. Based on these results, we concluded that seed of black raspberry can be useful in related field.

• Journal of the Korean Geotechnical Society
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• v.21 no.8
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• pp.107-116
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• 2005

Generally, when the stability of the geosynthetic reinforced soil slopes is analyzed, the required tensile forces of each reinforcement layers are calculated from total reinforcement forces which are necessary to retain the equilibrium state of slip mass in which the slip surfaces are assumed to be a linear or bilinear. It is assumed that the reinforcement forces are increased or constant with depth. However, according to the instrumented field data and laboratory model test results, the maximum tensile strain of reinforcement in a reinforced soil slope is developed in a certain elevation, not a bottom of the slope. In the concept of reinforced soil, postulated failure surfaces are the traces of the position in which the reinforcement tensile forces are maximum in the layer, and the reinforcement tensile forces are related to the stress state on the postulated failure surface. Therefore, in this study, based on the distribution of normal stress on the slip surface, a new method for the evaluation of required tensile forces is suggested and a number of the instrumented field data are analyzed by newly suggested method. As a result, it is shown that the newly suggested method produces relatively accurate reinforcement tension forces.

• Journal of the Korean Geotechnical Society
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• v.32 no.11
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• pp.43-50
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• 2016

A reinforcement is required to ensure the structural safety in case of railway embankment excavation under railway load. A large diameter soil nailing with concrete wall is applied as the reinforcement method instead of the conventional soil nailing system. In this study, a series of 3 dimensional numerical analyses are performed to investigate the optimum reinforcement considering 15 different conditions based on the length, lateral spacing, diameter, and inclination of the reinforcement. The interface between soil nail and perimetric grout is considered by means of cohesion, stiffness and perimeter of the grout. 0.3 m of reinforcement diameter is assessed as the most appropriate based on the economical viewpoint though ground displacement decreases with the increase of diameter, however the difference of displacement is negligible between 0.4 m and 0.3 m of diameter. Surface settlement, lateral displacement of wall, and stress of reinforcement are calculated and economic viewpoint to reinforce embankment considered. Consequently, the optimum reinforcement conditions considering those factors are evaluated as 3 m in length, 0.3 m in diameter, 1.5 m in lateral spacing, and 10 degree of inclination angle in the case of 3 m of excavation depth. Additionally, inclined potential failure surface occurs with approximately 60 degrees from the end of nails and the surface settlement and wall lateral displacement are restrained successfully by the large diameter soil nailing, based on the result of shear strain rate.

• Computational Structural Engineering
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• v.10 no.4
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• pp.165-175
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• 1997

In this paper, the stress, buckling and vibration analyses have been performed for several case with the spot weld stiffened rear side frame, the unstiffened rear side frame and the dual thickness laser weld rear side frame. For stress and vibration analyses, the clamped boundary condition with spring supports are used. But for the buckling analyses, the both ends simply supported boundary conditions are used. For the nummerical analyses, ANSYS 5.0 code is adopted. Maximum stress of the spot weld stiffened rear side frame occurs in the main frame and is 80.9 MPa. Maximum strain is 501 .mu.. The maximum stress of the dual thickness laser weld rear side frame of 1.8mm thickness structure is equal with the stress of spot weld stiffened frame. The weight of dual thickness laser weld frame can be reduced about 17.2%. For the stiffened spot weld rear side frame with both ends simply supported boundary conditon, the bucking load is 52.54 kN. When the thickness of the dual thickness laser weld rear side frame become 1.9mm thickness structure, the buckling load of the stiffenerd rear side frame is equal to that of dual thickness laser weld frame. The reduction of the structure weight is about 5%. The fundamental natural frequency of the stiffened spot weld rear side frame for bending mode is 163.6 Hz and that of the dual thickness laser weld rear side frame is 179.8 Hz.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.37 no.1
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• pp.1-7
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• 2017

In general, the large loads which are applied from explosion, impact, earthquake and wind at a short time caused the materials of structures to large deformations, rotations and strains locally. If such phenomena will be analyzed, hydrocodes which can be considered fluid-structure interaction under computational continuum mechanics are inevitably needed. Also, the explosion mechanism is so complicated, it is reasonable that the behaviors of structure are predicted through explosion analyses and experiment at the same time. But, unfortunately, it is true that explosion experiments are limited to huge cost, large experiment facilities and safety problems. Therefore, in this study, it is shown that the results of explosion analyses using the AUTODYN are agreed with those of existing explosion experiments for reinforced concrete slabs within reasonable error limits. And the explosion damage of the same reinforced concrete slab are assessed for quite different reinforcement arrangement spacings, concrete cover depths, and vertical reinforcements. From the explosion analyses, it is known that the more the ratio of slab thickness to reinforcement arrangement spacing is increased, and small-diameter reinforcements are used than large-diameter reinforcements on the same reinforcement ratio, and vertical reinforcements are used, the more the anti-knock capacities are improved.