• Mycobiology
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• v.46 no.3
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• pp.287-295
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• 2018

In this study, we evaluated the effect of different temperatures (10, 20, 30, and $40^{\circ}C$) and relative humidities (RHs; 12, 44, 76, and 98%) on populations of predominant grain fungi (Aspergillus candidus, Aspergillus flavus, Aspergillus fumigatus, Penicillium fellutanum, and Penicillium islandicum) and the biocontrol activity of Pseudomonas protegens AS15 against aflatoxigenic A. flavus KCCM 60330 in stored rice. Populations of all the tested fungi in inoculated rice grains were significantly enhanced by both increased temperature and RH. Multiple linear regression analysis revealed that one unit increase of temperature resulted in greater effects than that of RH on fungal populations. When rice grains were treated with P. protegens AS15 prior to inoculation with A. flavus KCCM 60330, fungal populations and aflatoxin production in the inoculated grains were significantly reduced compared with the grains untreated with strain AS15 regardless of temperature and RH (except 12% RH for fungal population). In addition, bacterial populations in grains were significantly enhanced with increasing temperature and RH, regardless of bacterial treatment. Higher bacterial populations were detected in biocontrol strain-treated grains than in untreated control grains. To our knowledge, this is the first report showing consistent biocontrol activity of P. protegens against A. flavus population and aflatoxin production in stored rice grains under various environmental conditions of temperature and RH.

• Mycobiology
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• v.45 no.3
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• pp.213-219
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• 2017

In our previous study, three bacterial strains, Bacillus megaterium KU143, Microbacterium testaceum KU313, and Pseudomonas protegens AS15, were selected as effective biocontrol agents against Aspergillus flavus on stored rice grains. In this study, we evaluated the inhibitory effects of the volatiles produced by the strains on A. flavus growth and aflatoxin production on stored rice grains. The three strains significantly reduced mycelial growth of A. flavus in dual-culture assays compared with the negative control strain, Sphingomonas aquatilis KU408, and an untreated control. Of these tested strains, volatiles produced by B. megaterium KU143 and P. protegens AS15 markedly inhibited mycelial growth, sporulation, and conidial germination of A. flavus on agar medium and suppressed the fungal populations in rice grains. Moreover, volatiles produced by these two strains significantly reduced aflatoxin production in the rice grains by A. flavus. To our knowledge, this is the first report of the suppression of A. flavus aflatoxin production in rice grains using B. megaterium and P. protegens volatiles.

• Mycobiology
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• v.46 no.1
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• pp.52-63
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• 2018

In our previous studies, Bacillus megaterium KU143, Microbacterium testaceum KU313, and Pseudomonas protegens AS15 have been shown to be antagonistic to Aspergillus flavus in stored rice grains. In this study, the biocontrol activities of these strains were evaluated against Aspergillus candidus, Aspergillus fumigatus, Penicillium fellutanum, and Penicillium islandicum, which are predominant in stored rice grains. In vitro and in vivo antifungal activities of the bacterial strains were evaluated against the fungi on media and rice grains, respectively. The antifungal activities of the volatiles produced by the strains against fungal development and population were also tested using I-plates. In in vitro tests, the strains produced secondary metabolites capable of reducing conidial germination, germ-tube elongation, and mycelial growth of all the tested fungi. In in vivo tests, the strains significantly inhibited the fungal growth in rice grains. Additionally, in I-plate tests, strains KU143 and AS15 produced volatiles that significantly inhibited not only mycelial growth, sporulation, and conidial germination of the fungi on media but also fungal populations on rice grains. GC-MS analysis of the volatiles by strains KU143 and AS15 identified 12 and 17 compounds, respectively. Among these, the antifungal compound, 5-methyl-2-phenyl-1H-indole, was produced by strain KU143 and the antimicrobial compounds, 2-butyl 1-octanal, dimethyl disulfide, 2-isopropyl-5-methyl-1-heptanol, and 4-trifluoroacetoxyhexadecane, were produced by strain AS15. These results suggest that the tested strains producing extracellular metabolites and/or volatiles may have a broad spectrum of antifungal activities against the grain fungi. In particular, B. megaterium KU143 and P. protegens AS15 may be potential biocontrol agents against Aspergillus and Penicillium spp. during rice grain storage.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.36 no.3
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• pp.271-279
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• 1991

The changes in fat acidity of brown rice and eating quality of rice during long term grain storage of unhulled rice were evaluated for the government managed rices. The Tongil type rice grains had consistently significantly lower fat acidity during three and half year storage than the Japonica type rice. On sensory evaluation of the fresh milled cooked rice the eating quality of the long term stored rices, 1987, 1988, and 1989 produce Tongil did not differ much each other even though increase in fat acidity by year of storage. The Japonica rices produced in 1989 and 1990 in different locations and stored there also did not differ much in eating quality. The grains stored in storehouses of super grade did not differ significantly from those stored in the first grades, but the grains stored (4 $^{\circ}C$, RH 50%) grains have kept the eating quality almost same as the fresh grains even after two and half years. A higher degree of milling did not improve significantly the sensory taste of cooked rice of the grains long term stored.

• Mycobiology
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• v.44 no.2
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• pp.67-78
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• 2016

Rice contaminated with fungal species during storage is not only of poor quality and low economic value, but may also have harmful effects on human and animal health. The predominant fungal species isolated from rice grains during storage belong to the genera Aspergillus and Penicillium. Some of these fungal species produce mycotoxins; they are responsible for adverse health effects in humans and animals, particularly Aspergillus flavus, which produces the extremely carcinogenic aflatoxins. Not surprisingly, there have been numerous attempts to devise safety procedure for the control of such harmful fungi and production of mycotoxins, including aflatoxins. This review provides information about fungal and mycotoxin contamination of stored rice grains, and microbe-based (biological) strategies to control grain fungi and mycotoxins. The latter will include information regarding attempts undertaken for mycotoxin (especially aflatoxin) bio-detoxification and microbial interference with the aflatoxin-biosynthetic pathway in the toxin-producing fungi.

• Korean Journal of Food Science and Technology
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• v.7 no.1
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• pp.7-10
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• 1975

A series of experiment was undertaken to pursue the possibility of aflatoxin accumulation in stored grains which were artificially contaminated with A. flavus var. columnaris isolated from deteriorated rice in Korea. In storage of rice grain, aflatoxin formation needed relative humidity over 80%, once accumulated aflatoxin was degraded by other microbial growth maintaining 50% level and the absence of other microoganisms brought about the remarkable accumulation of aflatoxin. The formation of aflatoxin in grains was increasing in the order of soybean, peanut, corn, wheat, barley, foxtail millet, rice, polished wheat, mung bean and great millet and it appears to be affected by the size and oil content of grains.

• Journal of Biosystems Engineering
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• v.5 no.1
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• pp.33-42
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• 1980

This study was carried out to investigate the actual environmental paddy storage conditions in conventional Korean farm warehouses. The paddy storage conditions by the locations and sizes of warehouses were figured out. The effectiveness of the small insulated box for storing paddy was also examined. Observations were made at sixteen different warehouses located in Moonsan-Myeon, Jinyandg-gun, Gyungnam Province from July lst to September 28 th, the period which was considered to be the worst for storing grains. The results are as follows ; 1. the average temperatures of rough rice and air inside the warehouse were 0.9 and 0.7$^\circ$ higher than the outside air temperature. 2. The average relative humidity in warehouses was 1.5percent higher than that of the atomosphere. This fact resulted in the increased moisture content of grains. Therefore, the ventilation for equalizing the relative humidity of inside and outside of warehouses was required. 3. The dry matter of stored rough rice was decreased by 1.1 percent in average druing the observation period. In order to reduce the dry matter loss, application of new grain storing method ;hermetic storage or filling inert gas storage, was highly recommended. 4. Environmental conditions for storing rough rice in a warehouse located in a sloping site are, in general , better than those of a warehouse located in a flat site. But as far as the dry matter of rough rice was concerned, above situation is not always satisfactory. Because it is fairly frequent to observe the higher rate of moisture absorption by grains stored in a warehouse located in a sloping site. 5. Environmental conditions for storing rough rice in a large-scale warehouse were better than those in a small size warehouse. Therefore, it is advisible for farmers to store their grins in the large-scale warehouses commonly used by villagers. 6. It was undesirable to store rough in a insulated box.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.37 no.3
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• pp.299-303
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• 1992

Methods for harvesting and storage of rice were also originally introduced from the ancient China like other practices of agricultural techniques. Thus, optimum time or rice harvesting was adopted by appropriate draining to prevent the losses, and efficient tools were utilized for harvesting and manufacturing. In addition, our unique and original techniques for rice storage were learned and handed down, which prevented winds and rains, and allowed good ventilation. In the 15th and 16th centuries, treatment of smartweed extracts as emphasized to prevent insect damage for stored barley and rice cutting immediately at harvest was encouraged to prevent shattering of rice grains. In the 18th and 19th centuries, means for prevention of damages by water, insects and rats when grains stored in the field were suggested. Also, studies for ecology of various rice cultivars and storage techniques for a long period of time were necessarily emphasized to assume the optimum time of harvest regionally and to cooperatively work timely.

• Journal of Applied Biological Chemistry
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• v.66
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• pp.289-298
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• 2023

Stored rice grain undergoes physical and chemical deteriorations over time. As temperature and moisture content are important factors affecting to the denaturation of rice, it is important to store rice at a low temperature and hermetic condition. From a microscopic point of view, many studies have already reported how proteins and lipids were denatured within rice grain. Meanwhile, the weight loss of rice is currently observed at actual storage sites and can occur for diverse reasons. In this study, it was assumed that the decomposition process of polysaccharides, known as the main component of rice, plays an important role in its weight loss. In specific, the roles of enzymes were also evaluated. Our interest is in the major polysaccharides within a rice grain such as starch as well as within a rice endosperm cell wall. It is suspected that the weight loss of rice grains during storage seems to associate with the degradation of amylose and amylopectin. Nevertheless, it should be also speculated the correlating effect of other components such as proteins and lipids.

• Journal of the East Asian Society of Dietary Life
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• v.26 no.5
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• pp.457-465
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• 2016

Effects of soaking time on the physicochemical and structural characteristics of waxy rice flours and starches purified from flours using the alkaline steeping method were investigated. Korean cultivar Sinseonchal waxy rice was washed and soaked in tap water (1:2 w/w) and stored at room temperature for 15 days. On each day of soaking for 0, 1,2 3, 5, 10, and 15 days, pH of soaking water was measured and rice grains were dried, ground, and passed through 100 mesh sieve. The pH was reduced to 3.90 by day 5 and increased to 4.60 by day 15. The protein and ash contents, swelling powers and solubilities of flours and starches decreased with increasing soaking time. The water-binding capacities increased while trends were not similar to soaking time. The flour particle size distribution ranged from two to four peaks with increasing soaking times. Starch granule size decreased with increasing soaking time. The peak, trough, and final viscosities of flours and starches showed similar trends until 10 days and 15 days, respectively. The starches presented higher viscosities than the flours. The branch chain length distributions of amylopectin of starches showed an increaseed DP6~12 portion and decreased DP13~24 portion with increasing soaking time of waxy rice grains.