• Title/Summary/Keyword: storage protein

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Effect of Persimmon Peel (Diospyros kaki Thumb.) Extracts on Lipid and Protein Oxidation of Raw Ground Pork During Refrigerated Storage

  • Choe, Ju-Hui;Kim, Hack-Youn;Kim, Cheon-Jei
    • Food Science of Animal Resources
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    • v.37 no.2
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    • pp.254-263
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    • 2017
  • The inhibition effect of persimmon peel extracts (PPE) (0.05(PPE-0.05), 0.1(PPE-0.1), and 0.2 g(PPE-0.2) per meat sample) on lipid and protein oxidation of pork patties during chilled storage for 12 days were investigated and compared to ascorbic acid (As-0.05) and butylhydroxytoluene (BHT) (BHT-0.01). The meat samples treated with PPE had greater (p<0.05) $a^*$ values comparing control in raw pork patties meat from day 4 of storage. The addition of PPE at all concentrations on meat samples effectively inhibited the formation of oxidation products as shown by decreasing conjugated dienes (CD), peroxide values (POVs), thiobarbituric acid reaction substances (TBARS), and carbonyl content during chilled storage for 12 d. The PPE-0.2 and BHT-0.01 had the lowest in decrease rate of free thiol content (0.24 and 0.22 times) during chilled storage. Therefore, results of this study suggest that PPE can be considered a potential antioxidant against lipid and protein oxidation of raw meat products.

Effects of Whey Protein Injection as a Curing Solution on Chicken Breast Meat

  • Ha, Jung-Heun;Lee, Ju-Ho;Lee, Jae-Joon;Choi, Yang-Il;Lee, Hyun-Joo
    • Food Science of Animal Resources
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    • v.39 no.3
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    • pp.494-502
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    • 2019
  • The quality characteristics and storage stability of chicken breast meat (CBM) was investigated following the injection of whey protein (WP) as a curing ingredient. The moisture content of CBM decreased with increasing concentration of WP. The highest concentration of WP (7%) resulted in the lowest moisture and fat content and the highest protein content of CBM. Injection of WP elevated the pH and water holding capacity (WHC) of CBM. The cooking loss of CBM was significantly decreased with WP injections of 3% and higher. All WP injections increased the $L^*$ of the CBM but decreased the $a^*$ and $b^*$. WP injection increased the springiness, cohesiveness, and chewiness and decreased the hardness of the CBM. WP injection increased 2-thiobarbituric acid reactive substances (TBARS) after 3 and 7 days of storage. The volatile basic nitrogen (VBN) content of the CBM increased with increased concentrations of WP. The total microbial count (TMC) of CBM injected with WP was higher initially and after 3 days of storage. Our results showed WP injection improved the WHC of CBM but decreased the storage stability by increasing TBARS, VBN and TMC.

ON FARM DEMONSTRATION OF VARIOUS STORAGE METHODS FOR UREA TREATED WHEAT STRAW

  • Khan, A.G.;Ullah, W.;Azim, A.;Ali, A.
    • Asian-Australasian Journal of Animal Sciences
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    • v.9 no.3
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    • pp.281-285
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    • 1996
  • On farm demonstration of urea treatment (5 kg urea dissolved in 60 litre water/100kg) of straw was performed at 6 different sites and treated straw was stored by three different methods i.e., plastic covered, mud plastered and existing farmers technique (mud plastered on the top and open from sides) to determine the best storage method in field. Untreated and treated samples were taken after 5 week storage period and subjected to crude protein, crude fibre and cell wall constituents analysis. In situ dry matter digestibility of straw was measured by nylon bag technique in buffalo bulls. Crude protein content increased by 100 to 153 percent in treated straw stored by different methods. Maximum increase in crude protein of treated straw was noticed in mud plastered method. Urea treatment of straw resulted in significant decrease in crude fibre contents in all the storage methods. Treatment of straw enhanced the in situ digestibility by 25-49 percent and maximum digestibility (53%) was found in mud plastered storage method. It was concluded that the mud plastered storage method for urea treated straw was found to be the best at farm level.

USE OF MOLD INHIBITOR FOR FEED STORAGE AND IMPROVED CHICK PERFORMANCE

  • Nahm, K.H.
    • Asian-Australasian Journal of Animal Sciences
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    • v.4 no.3
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    • pp.285-291
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    • 1991
  • Two experiments were conducted to evaluate the effect of mold inhibitor in the ration which had two different protein levels (18% and 12%) and two different particle sizes (80 or 40% of the particles in the ration less than 1.19 mm). The experimental diets with ave. 12.7% moisture which were treated at the level of 0.1% mold inhibitor were stored under 85% humidity and at $29{\pm}1^{\circ}C$ for 10 to 40 days. In experiment 1, after 40 days of storage the $CO_2$ production in the feed treated with mold inhibitor was higher (p < 0.01) than when 40% of the ration's panicle size was 1.19 mm. Aflatoxin production in the experimental diet with mold inhibitor was affected (p<0.05) by the levels of protein and the different particle size ranges after 40 days storage. The interaction of protein levels and particle size ranges on the anatoxin and $CO_2$ production was significant (p<0.05) at 40 days storage. In experiment 2, there was a decrease in total body weight gain and total feed intake observed in chicks fed the untreated diet of 18% protein with 40% of the particles in the ration less than 1.19 mm stored for 40 days. Feed conversion was depressed (p<0.05) in the chicks fed the untreated diets of both particle sizes. Particle size X types of feed interaction in feed conversion was significant (p<0.05).

Purification and Characterization of Storage Proteins from the Mulberry Longicorn Beetle, Apriona germari Hope

  • Yoon, Hyung-Joo;Kim, Seong-Ryul;Jin, Byung-Rae;Lee, Sang-Mong;Moon, Jae-Yu;Mah, Young-Il;Soh, Hung-Dae
    • International Journal of Industrial Entomology and Biomaterials
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    • v.2 no.2
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    • pp.161-166
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    • 2001
  • The storage proteins of the mulberry longicorn beetle, Apriona germari Hope, were purified and characterized. Three kinds of storage protein (SP1, SP2 and Sp3) were purified from the last instar larval hemolymph of A. germari by the FPLC techniques, anion exchange chromatography and gel permeation chromatography. The SP1, SP2 and SP3 have a native molecular weight of 480, 440 and 420 kDa, respectively. In the SDS-polyacrylamide gel electrophoresis analysis, these storage proteins are composed of a single protein subunit with molecular weight of 90, 85 and 80 kDa, respectively. This result showed that the storage proteins are hexameric protein. The SP1 and SP2 were stained with Schiffs reagent, but SP3 was not stained. It can be assumed that SP1 and SP2 are glycoprotein. Western blot analyses using the each of polyclonal antiserum against purified SP1, SP2 and SP3 showed that the three antibodies reacted with the each of SP bands, respectively. Also, antibodies against SP1 and SP3 cross-reacted with the SP3 and SP1, respectively. However, SP2 was not cross-reacted with these two antibodies. Also, antiserum against SP2 did not cross-reacted with the SP1 and SP3.

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Quality Change in Precooked Sardine during Frozen Storage (자숙 정어리육의 동결저장중의 품질변화)

  • SUH Jae-Soo;LEE Kang-HO;JO Jin-HO
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.16 no.2
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    • pp.117-124
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    • 1983
  • Qualify changes of the precooked frozen sardine (Sardinops melanosticta) during frozen storage were investigated by measuring extractable protein, expressible drip, available lysine and lipid oxidation as peroxide value. Fresh sardine was dressed, washed in chilled water, cooked in boiling water to have $55^{\circ}C\;and\;70^{\circ}$ at the center of the body, frozen at $-40^{\circ}C$, and finally stored at $-20^{\circ}C$ for 84 days. The quality factor mentioned above were determined in both ordinary and dark muscle at 14 day intervals through the period of storage. When cooked at $70^{\circ}C$, the changes in expressible drip were less than that of raw and the one cooked at $55^{\circ}C$. In observation of the extractability of muscle protein, no great change in extractable sarcoplasmic protein was observed, the extractable myofibrillar protein, however, showed a tendency to decrease during the period of frozen storage, accompanying the increase of the alkali-soluble protein. That was more excessive in ordinary muscle than dark muscle. Lipid oxidation of dark muscle was faster than that of ordinary muscle. Acid value was not changed, and peroxide value of the samples cooked at $70^{\circ}C\;and\;55^{\circ}$ was higher than that of raw at the early stage of the storage, after 40-50 day storage, it became lower than that of raw muscle.

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Change of Physicochemical Quality According to Its Storage Temperature in Garlic (Allium sativum L.) (마늘의 저장온도에 따른 이화학적 품질변화)

  • 장현세;홍경훈
    • Food Science and Preservation
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    • v.5 no.2
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    • pp.119-122
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    • 1998
  • This study was conducted to blow the effect of postharvest physiological changes on garlic quality according to its ecotypes and storage temperatures. The changes of water, total soluble solids, crude stein, md total fructans were measured and the rates of respiration and sprouting were analyzed during storage at 20$^{\circ}C$ and 30$^{\circ}C$. The decrease of water content and the increase of total soluble solids were reversely appeared during garlic storage. The crude protein content was gradually increased during storage but total fructan content was decrased. The respiration late was maximized at 60days after storage and the spouting rate was gradually increased. In the aspect of ecotypic characteristics, the water content, fructan content and sprouting rate were higher in 'Namdo' cultivar than those of southern type. The high storage temperature (30$^{\circ}C$) controlled spouting and loss of fructan, and it was effective to maintain the garlic quality.

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The effects of soybean oil and cyclodextrin on the quality characteristics and storage of frozen soy yogurt prepared from proteolytic soy protein isolate (콩기름과 cyclodextrin 첨가량이 효소처리한 분리대두단백으로 제조한 frozen soy yogurt의 품질특성 및 저장성에 미치는 영향)

  • Lee Sook-Young;Park Mi-Jung
    • Korean journal of food and cookery science
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    • v.21 no.1 s.85
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    • pp.18-23
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    • 2005
  • The effects of soybean oil and cyclodextrin were studied on the quality characteristics and storage of frozen soy yogurt prepared from proteolytic soy protein isolate. The viscosity and overrun of frozen soy yogurt were improved by the addition of soybean oil and cyclodextrin. Upon the addition of cyclodextrin, there was a decrease in the peroxide value of yogurt with subsequent increase by the addition of soybean oil during frozen storage. During the process of storage, there was an increase in the acetone and diacetyl contents, but methanol, ethanol and butanol contents decreased. However, the value of hexanal content was not consistent in the obtained results. Sensory characteristics of frozen soy yogurt such as mouth feel, and overall quality were improved by the addition of cyclodextrin and remained the same frozen storage at $-20^{\circ}C$ for 30 days.

Storage characteristics of frozen soy yogurt Prepared with different proteolytic enzymes and starter cultures (단백분해효소와 Starter Culture의 종류에 따른 frozen soy yogurt의 저장성)

  • Lee Sook-Young;Lee Jung-Eun
    • Korean journal of food and cookery science
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    • v.21 no.2 s.86
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    • pp.217-224
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    • 2005
  • The storage characteristics of frozen soy yogurt prepared with hydrolyzed soy protein isolates were evaluated. In order to facilitate lactic fermentation bacteria grow and produce lactic acid as fast rate as possible, soy protein isolate(SPI) was hydrolyzed using two kinds of proteases; bromelain and a-chymotrypsin. The cultural systems employed thereafter for lactic fermentations were Bifidobacterium bifidum or B. bifidum and Lactobacillus bulgaricus. The viable cell counts, normal- and bile acid tolerances from the mixed cultures of B. bifidum and L. bulgaricus decreased sharply during the initial first 3 days of frozen storage and then showed a gradual decrease thereafter. Melt-down percent of the all frozen products have been favorably affected as was shown by less melting at raised testing temperature during 28 days of frozen storage except for the initial 3 days during which a minor change has been observed. Among the various volatile flavor components, the contents of acetaldehyde, acetone, diacetyl and methanol generally increased during the frozen storage. In sensory test, the frozen soy yogurt prepared with a-chymotrypsin and mixed culture of B. bifidum and L. bulgaricus was the most desirable, the highest scores in sourness, bitterness and mouthfeel.

Effects of storage temperature on quality characteristics of texturized vegetable protein

  • Seul Lee;Sun Young Jung;Mi Sook Seo;Chan Soon Park
    • Food Science and Preservation
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    • v.31 no.1
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    • pp.46-63
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    • 2024
  • This study evaluated the impact of storage temperature on the quality characteristics of texturized vegetable protein (TVP). TVP was prepared by mixing defatted Daewon soybean flour at 80℃, gluten, and corn starch in a 5:3:2 ratio, which was then extruded at a screw speed of 250 rpm and a barrel temperature of 190℃ with moisture addition at 9 rpm. Subsequently, the extruded TVP was vacuum-sealed in polyethylene packaging and stored at -20℃, 0℃, and 4℃ for 9 days. Texture analysis revealed that the curing rate followed 4℃ > 0℃ > -20℃ sequence. No significant color variation was observed across the storage conditions, although water content increased at all temperatures. Notable changes were detected in moisture absorption capacity (%) and solid leaching (%), following the order of -20℃ > 0℃ > 4℃. The turbidity of the solution released during cooking varied, with the highest to the lowest sequence being -20℃ > 4℃ > 0℃, while pH levels remained neutral. Regarding free amino acids, sweetness and textural quality improved with storage across all temperatures, whereas bitterness components diminished at 4℃. The study suggests that refrigerated storage at 4℃ is a viable method for distributing TVP, which was previously distributed only in a frozen and dry state.