• Title/Summary/Keyword: stomata.

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Cytological and Morphological Characterization of Anther Derived Plants from Sweet Pepper (Capsicum annuum L.) cv. 'Special'

  • Shrestha, Surendra Lal;Luitel, Binod Prasad;Lee, Taek Jong;Kang, Won Hee
    • Korean Journal of Breeding Science
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    • v.42 no.5
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    • pp.431-438
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    • 2010
  • Anthers of sweet pepper $F_1$ cultivar 'Special' were cultured on Dumas De Vaulx (C medium), supplemented with $0.1mgL^{-1}$ 2, 4-D and $0.1mg{\cdot}L^{-1}$ kinetin with 3% sucrose, and 0.32% phytagel. The calluses obtained were further sub-cultured on Murashige and Skoog (MS) medium without growth regulators for regeneration. Regenerated plantlets were grown in plastic pots under plastic house and characterized their cytological and morphological characters in spring, 2008. Twenty percent plantlets were identified as haploid plants after chromosome and ploidy analysis. Haploid plants contained 12 chromosomes, high stomatal density with small stomatal length as compared to diploid plants. Stomatal length in haploids was 23.3% smaller than diploids. Haploid plants were characterized as small leaf and petiole size, poor vigor, thin stem and short plant height, short internodes and small flower buds, fruit size and fruit weight as compared to diploid plants and most of the haploid fruits were seedless. SP55, SP62, SP68, SP72 and SP77 are found high yielding double haploids with high total soluble content (8.6, 8.7, 9.2, 9.1 and $9.8^{\circ}Brix$, respectively) and desirable fruit shape, and recommended them to exploit as inbred lines for heterosis breeding.

Efficiency of Removal of Indoor Pollutants by Pistia stratiotes, Eichhornia crassipes and Hydrocotyle umbellata

  • Park, Hye-Min;Lee, Ae-Kyung
    • Journal of People, Plants, and Environment
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    • v.23 no.1
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    • pp.15-21
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    • 2020
  • In this study, we compared efficiency of different aquatic plants in removing indoor pollutants and examined their potential to purify indoor air. Two liter of water in chamber was used as the control, while the other chambers containing water lettuce (Pistia stratiotes), water hyacinth (Eichhornia crassipes), and water coin (Hydrocotyle umbellata) were used as treatment groups. Temperatures inside all the chambers were maintained between 20 ℃ and 23 ℃. Humidity in the chambers with aquatic plants increased by 30% and 50% control respectively. The removal of formaldehyde per unit leaf area was examined in each aquatic plant. It turned out that water hyacinth removed the highest amount of formaldehyde, followed by water lettuce and water coin. Both water hyacinth and water lettuce increased the amount of removal of formaldehyde until the end of the experiment. In the case of airborne dust (PM 10) and fine dust (PM 2.5), water coin, which had the highest number of leaves, removed more PM 10 and PM 2.5 than the other aquatic plants, with statistically significant difference. In addition, both water coin and water hyacinth smoothly opened and closed stomata before and after the experiment. Consequently, as the aquatic plants were effective in controlling humidity and removing pollutants, they can be used as air purifying plants.

Prediction of the Vase Life of Cut Lily Flowers Using Thermography

  • Lee, Ja Hee;Choi, So Young;Park, Hye Min;Oh, Sang Im;Lee, Ae Kyung
    • Journal of People, Plants, and Environment
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    • v.22 no.3
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    • pp.233-239
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    • 2019
  • This study was conducted in order to predict the vase life of cut lily 'Woori Tower' flowers using a non-destructive thermal imaging technique. It was found that the temperature of cut lily flowers was maintained at 20℃ and was slightly lower than the air temperature until they bloomed. On the 11th day, when flowers bloomed, the temperature of leaves and flowers was measured to be 18.75±0.38℃ and 19.23±0.32℃ respectively, and their difference with ambient temperature was over 3℃. The flower temperature increased slightly when the vase life of cut lily flowers ended, and the temperature difference between the air and leaf temperature (1.77℃) and between the air and flower temperature (1.39℃) got smaller. No visible aging symptom was observed, but it was found that the temperature had risen due to water losses and less functional stomata. The vase life of cut lily flowers can be predicted based on changes in temperature and it will be also possible to predict the potential quality and vase life of cut flowers before harvesting them in greenhouses.

Variation of Genus Ilex in Korea and their Ornamental Values (Ilex속(屬) 수목(樹木)의 유전변이(遺傳變異)의 분석(分析)과 조경학적(造景學的) 이용가치(利用價値)의 조사(調査) 연구(硏究))

  • Yim, Kyong Bin
    • Journal of Korean Society of Forest Science
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    • v.42 no.1
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    • pp.1-38
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    • 1979
  • The woody species of Genus Ilex which are endemic to Korea are distributed on limited area due to solely temperature factor. There is some differences according to species, however in general, the evergreen Ilex are found along southern coastal area of Korean Peninsula and near islands where the cold index does not exceed $-5^{\circ}C$. But Ilex macropoda and the variety, only deciduous ones, are grown in temperate zone of the peninsula and some islands. The list of Ilex species of Korea are as follows. Ilex cornuta Lindley et Pax., I. crenata Thunb. var. microphylla Max., I. crenata Thunb., I. rotunda Thunb., I. macropoda Miq., I. macropoda Miq. var. pseudo-macropoda Loensner, I. integra Thunb. The author surveyed the populations of Ilex species as many as possible and data of some characters such as leaf shape, spine, fruit shape, stomata density, sex ratio in natural communities, etc. are collected. Almost all the Ilex species in Korea show sporadic distribution. This means quite small sized populations isolate distantly each other eliminating the change of gene exchange in between. Particularly Ilex conuta and I. crenata show the morphological differentiation among populations as well as significant individual variation within a population. These were true with such characteristics, leaf shape, leaf dimension, leaf margin, fruit shape, spine, and stomata density. The founded are that the fruit length and the stomata density counted on the beneath surface of leaves of Ilex cornuta increased with the decrease of latitude. These are naturally closely related with the cold index values. The table shown below indicates the correlation between mean stomata density per $0.3642mm^2$ and cold index values. These relation however were not observed on Ilex crenata. The most dominated natured in relation to individual variation were outline of leaf, the number of marginal spine, the shape of leaf cross section and the degree of luster of the upper leaf surface. As shown in photos 5~7, these variations are agreed at a glance. There are reports that the development of marginal spines in some Ilex species is associated with the juvenility and topophysis. In present study, these two factors were neglected because of the intended sampling procedure. Of Ilex rotunda, population difference with the characteristics of leaf length is recognized but not for leaf width, petiole length, and fruit size. However, individual variations within a population were significantly large. In case of Ilex integra, only individual differences within population were calculated statistically for such characteristics as leaf length, leaf width, and petiole length. As to natural population, the sex ratio was 1:2 (female to male) for Ilex cornuta, and 1:1 for Ilex crenata. The tendency of more male than female in I. cornuta was agreed to other observations. Preparing the tip cutting of length 10cm, and treating with IBA, then attaching earth ball to the cut end, very successful rooting percentages were obtained. Asexual propagation has the advantages of maintaining the heterozygosity of existing varieties and overcoming the difficulties of delayed seed germination frequently encountered with Ilex species. Considering a great deal of variation in morphological traits, a good possibility of selection breeding for decorative and ornamental purposes exists. At present, these evergreen Ilex are ignored by local people as nuisance weedy shrubs. So the proper protection measures should promptly be taken.

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Histological Perturbations of Crop Leaves after Exposure to Simulated Acid Rain;II. For rice, soybean, barley, and radish (인공산성(人工酸性)비에 의한 농작물(農作物) 잎의 조직형태(組織形態) 변화(變化)에 관(關)한 연구(硏究);II. 벼, 콩, 보리, 무에 관하여)

  • Lee, Jong-Sik;Kim, Bok-Jin;Jung, Goo-Bok
    • Korean Journal of Environmental Agriculture
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    • v.15 no.1
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    • pp.19-24
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    • 1996
  • To investigate the alterations of ultrastructure of leaves by acid rain, 10mm of SAR(Simulated Acid Rain, pH 2.0, 2.5, 2.7, 3.0, 6.0) were applied to 4 crops(rice, soybean, barley, and radish) at the two day interval. The symptoms of leaf damage by SAR were observed by naked eyes and SEM(Scanning Electron Microscope). The results are summarized as follow: Visible leaf injury were more serious in dicots than monocots such as rice and barley with the order of bean, radish, rice, barley. With the SAR treatment of pH 2.0, histological perturbation of trichome were developed in bean and radish. And with the SAR treatment of pH 2.5, stomata of all tested crops except rice were deshaped.

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Studies on a Tetraploid of Robinia pseudoacacia L. Selected in the Planted Forest (조림지(造林地)에서 선발(選拔)한 자연(自然) 4배체(倍體) 아까시나무(Robinia pseudoacacia L.)에 관(關)한 연구(硏究))

  • Kim, Chung Suk;Lee, Suk Koo
    • Journal of Korean Society of Forest Science
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    • v.14 no.1
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    • pp.39-44
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    • 1972
  • Cytological investigation was done on a tetraploid tree of Robinia pseudoacacia L. selected in the planted forest in Chilbo Mountain. From the results of observation of chromosomes on mitotic metaphase in leaves, it was confirmed that the mutant is tetraploid plant (2n=40). The external form of leaves of the spontaneous tetraploid was gigantic and dark green one in comparison with diploid. There was no difference in the size of guard cell and the number of stomata per unit area between the mutant and the diploid plant, however, length and thickness of wood fiber were increased as compared with that of diploid. The spontaneous gigas tetraploid showed growth performance as much as the colchicine induced gigas tetraploid.

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The Mechanism of Stomatal Closing by $H_2O_2$ in Epidermal Strips of Commelina communis L. (닭의장풀의 분리표피에서 $H_2O_2$에 의한 기공 닫힘기작)

  • 이준상;전방욱
    • Journal of Environmental Science International
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    • v.6 no.2
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    • pp.125-131
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    • 1997
  • The mechanism of stomatal closing in response to $O_2$ was indirectly investigated by using $H_2O_2$ which is the intermediate product of $O_2$ metabolites. Stomata in epidermal strips close in response to $H_2O_2$. The effect of $H_2O_2$ on stomatal closing was dependent on the concentration of $H_2O_2$. 10 ppm $H_2O_2$ showed a clear effect on stomatal closing and 1000 ppm $H_2O_2$ induced complete stomatal closing after the treatment of 3 hours. Stomatal closing by $H_2O_2$ in intact leaf was also observed by measuring the diffusion resistance with porometer. It was found that the stomatal closing by $H_2O_2$ was not mediated by $Ca^{2+}$, and that was a different result observed in stomatal closing by water stress. Reversely, $Ca^{2+}$ showed a great inhibition on stomatal closing. The leakage of K+ in epidermal strips was doubled in response to $H_2O_2$ when it was campared to the control. 10 ppm $H_2O_2$ decreased photosynthetic activity. Fv/Fm representing the activity of Photosystem II was reduced about 4 % in 10 ppm $H_2O_2$ and 8 % in 100 ppm $H_2O_2$ In the treatment of 1.5 hour. However, stomatal closing by 10 ppm $H_2O_2$ was reduced about 56 %. According1y, it can be suggested that stomatal closing by $H_2O_2$ is related with the decrease of photosynthetic activity, but it was chiefly induced by the change of the membrane permeability. Key words Commelina communis, stomatal closing, $H_2O_2$, $Ca^{2+}$, photosynthesis.

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Effects of Ozone Environmental Stress on Growth and Stomatal Response in the F2 Hybrid Poplar (Populus trichocarpa × Populus deltoides) (오존 환경(環境)이 잡종(雜種) 포플러의 생장(生長)과 기공개폐(氣孔開閉)에 미치는 영향(影響))

  • Woo, Su-Young
    • Journal of Korean Society of Forest Science
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    • v.87 no.1
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    • pp.50-56
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    • 1998
  • Thirty-six $F_3$ hybrid poplar (Populus trichocarpa${\times}$P. deltoides) clones were fumigated with ozone to select for ozone sensitive and resistant clones. Fumigation was applied for 6 to 8 hours each day for approximately 3 months at ozone concentrations of 90 to 115 ppb using by open-top chambers. Height, diameter, number of leaves, total biomass, biomass components, root/shoot ratios, leaf drop and stomatal response were investigated. In summary, ozone generally reduced height, diameter, number of leaves, total biomass, and root/shoot ratios. Ozone stress induced leaf drop and foliar senescence in trees. This study showed very low relationship between total biomass and stomatal conductance. Increased plant resistant to ozone is not always correlated with stomatal behaviour. Probably, characterization of biochemical and other physiological responses to ozone exposure can provide a better understanding of tree response to ozone environment.

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Effect of Changes of Leaf Water Content on Respiration and Photosynthetic Rate of Tobacco Varieties (엽중수분 변화가 잎담배 품종간 호흡과 광합성속도에 미치는 영향)

  • Seong-Kook Bae;Ryuichi, Ishii;Atsuhiko, Kumura
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.30 no.4
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    • pp.347-351
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    • 1985
  • The effect of leaf water content on apparent photosynthesis and respiration of tobacco plants(five varieties) was studied under the condition without the irrigation for 10 days after the plants were sufficiently watered on the first day. The wild race (N. longiflora) among varieties showed highest apparent photosynthesis (AP) and AP had a positive correlation with specific leaf weight. N. longiflora and Andongyeob were different in their AP from the other varieties significantly under the water stress condition. Respiration rate also decreased to be simillar to AP except slight increase at early stage of water deficit. The stomatal resistance and the mesophyll resistance increased in the stressed plants. The water stress resistant character seems to be mainly due to open stomata.

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Effect of Reflective Film Mulching on the Growth and Flowering of Antirrhinum majus L. 'Fujinoyuki' in Greenhouse Cultivation (시설재배에서 반사필름 멀칭이 금어초 생육 및 개화에 미치는 영향)

  • Kim, Wan-Soon;Huh, Kun-Yang;Cho, Il-Hwan;Woo, Yong-Hoe
    • Horticultural Science & Technology
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    • v.16 no.3
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    • pp.350-351
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    • 1998
  • This study was conducted to investigate the effect of reflective film(RF) mulching on the growth and flowering of snapdragon 'Fujinoyuki' in greenhouse cultivation. On the spectroradiometry of mulching materials in the wavelength zone of 300nm to 1100nm, 85% of total light source was reflected from RF, while over 95% was absorbed into black polyethylene film(BL). Under plant canopy, light, air temperature, and leaf temperature were higher on the RF mulching than BL, but soil temperature and soil heat flux were higher under the BL. Primary plant growth such as dry weight, stem hardness, lodging, and transpiration was superior when using RF mulching. RF mulching accelerated the plants to bloom about 12 days earlier with admirable cut flower quality.

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