• Korean Journal of Plant Tissue Culture
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• v.25 no.5
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• pp.347-351
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• 1998

Plant regeneration via organogenesis from leaf and stipule explants of micropropagated shoots of strawberry (Fragaria $\times$ ananassa cv. Suhong) was achieved. Leaf and stipule explants were detached from shoot-tip cultured shoots and cultured on MS medium with various combinations of BA and NAA under light or dark condition. Shoot regeneration from leaf explant was observed after 3 weeks in culture and was good at the high ratio of BA and NAA among various combination treatments. The highest shoot regeneration frequency from leaf explants was obtained with 1.0 mg/L BA and 0.1 mg/L NAA, in which 31.1% shoot regeneration frequency(1.7 shoots per leaf explant) was yielded. In case of stipule explants, shoot regeneration was largely affected by plant growth regulators during incubation under dark condition for initial 4 weeks but not under continuous light condition. The combination treatment with 2.0 mg/L BA and 0.1 mg/L NAA showed the most excellent shoot regeneration from stipule explants, where 44.4% regeneration frequency(4.0 shoots per explants) obtained. Regenerated shoots were rooted on MS medium with 0.1 mg/L NAA after shoot elongation, and the plantlets regenerated were transferred to soil mixtures with vermiculite and perlite for acclimation.

• Journal of Korean Society of Forest Science
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• v.72 no.1
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• pp.9-15
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• 1986

The taxonomy of the broad-leaf evergreen trees (4 genera, 5 species) of Lauraceae was studied by the morphological characteristics of stipules. The results obtained were summarized as follows; 1) The stipules of Lauraceae were arranged alternately with 5-31 stipules of simple leaf. The venations or stipules were basal reticulate and pinnated vein. The shapes of stipule blade were oblong, oblanceolate, rhomboid and oval. The stipule apex was acuminate and obtuse, the stipule base was attenuate, and the stipule margin was entire margin. The trichomes of stipules were sericeous, tomentose and villous. The five species of Lauraceae could be identified by the morphological characteristics of stipules. 2) The morphologically changing forms of stipule vein were classified into three groups; A form - the form of pinnate vein with the distinguished main vein (lozoste lancifolia), B form - the three vein grows into the form of basal reticulate vein (Neolitsea sericea, Cinnamomum japonicum and C. camphora), C form - the parallel vein grows into the form of basal reticulate vein (Marhilus thunbergii). 3) The changing forms of stipule's width from stipule "a" to "j" were three forms; (1) a>d>g>j form: C. japonicum, C. camphora and I. lancifolia, (2) a>cj,(aj,(a

• Journal of the Korean Institute of Landscape Architecture
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• v.23 no.1
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• pp.111-122
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• 1995

12 populatiDns of Ch indicum L. and it's variation antogenous in Tforea were selected throughout the country and their morphological characteristics were studied in this paper. The results are as follows. 1) The mean of the plant height is 97.3cm. Ch. indicunl is 76.5cm and Ch. boreale is 116. 6cm in height. The number of nodes is 58 on the average. The color of the stem is generally black-red in the case of Ch. indicum and is green for Ch. boreale. 2) The leaves of Ch. inficum are smaller In size and thicker than chose of Ch. boreale, and it has a stipule. This fact enables us to distinguish one species from the other, however, the Gyukpo-population of Ch. indicum has a large leaf, and Byunsan-population of Ch. boreale has a stipule so that the above fact is not an exact criterion on which to base one classification. 3) The size of the capitulum is 24.9mm for Ch. indicum and 15.6mm in the case of Ch. boreaje so that the difference between species is admitted. It was confirmed hat the size of the capitulum has positive correlation to that of the pedicel and has egative correlation to the number of flowers, so that of inflorescence, Ch. indicumhas corymb, and Ch. boreale has crowed corymb or umbel-type.The number of ligulates is 19 on the average, which no the difference being founnd between species and populations.4) The cluster analysis of morphological characteristics showed that Ch. indicum wasdivided into two groups, which was shown thought to be due to the difference of speciesbetween Ch. indicum and Ch. boreale. However, it could not be shown that their morphological characteristics explain a geographical relationship between populations

• Korean Journal of Plant Taxonomy
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• v.49 no.1
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• pp.1-7
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• 2019

Early leaves within the Viola albida complex were investigated by scanning electron microscopy in order to determine the morphological segments during morphogenesis. The early leaf development of V. albida var. albida could be morphologically divided into the eight stages in the following order: I, the initiation of shoot germination; II, the conical growth directionally of the leaf; III, the adaxial and abaxial formation of the leaf; IV, the initiation of the stipule; V, the formation of a transitional zone between the leaf blade and petiole; VI, the expansion of the upper part of the leaf blade; VII, the formation of almost all parts of the early leaf; VIII, the early simple leaf. Viola albida var. takahashii differs from V. albida var. albida by additional stages, i.e., V-1, the initiation of the first lateral lobe at the both lateral parts of the leaf after the stage V and an early lobed leaf. Viola albida var. chaerophylloides is also distinguished from two taxa by two developmental features, V-2, the initiation of a second lateral lobe below of the first lateral lobe, and an early palmately compound leaf. These findings suggest that the Viola albida complex would be in the process of peramorphosis, showing developmental changes in a chain of events, leading to a different leaf shape. These data would also be useful for isolating genes that give rise to different leaf morphogenesis outcomes among the taxa in the Viola albida complex.

• Journal of Environmental Science International
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• v.11 no.10
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• pp.1001-1009
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• 2002

Vegetative characters (e.g. habitat, root, ramification and habit of stem, phyllotaxy, leaf sape, shape of leaf margin, shape of division and lobe of division, number of division, thick and texture of leaf and shape of stipule) on 35 species of Asian Geranium were reviewed to discussion propriety of taxonomic character and evolutionary trends. Wilfordii group is characterized by three divided leaf, Farreri group was grown a high altitude, and Maculatum group is grouped by life cycle of anneal. Arrangement state of leaf is alternate type G, tripartitum, G. eriostemon, and G. erianthum is thought the more primitive than the other taxon, and regarded as more advanced group the sibiricum group and pseudosibiricum group which divided of leaf deeply. Shape of leaf, division degree of lobes, shape of leaf margin and number of division are considered a good identification characters because width of change are fixed between population.