• Title/Summary/Keyword: sterilization method

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Effects of Sterilization for Quality Control and Content of baicalin in Scutellariae Radix (멸균법에 따른 황금의 미생물학적 품질보증과 바이칼린 함량에 미치는 영향)

  • Jeong, Choon-Sik
    • Korean Journal of Pharmacognosy
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    • v.36 no.3 s.142
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    • pp.220-223
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    • 2005
  • The purposes of this study was to develop the best one among the methods using dry heat, gamma irradiation and alcohol gas, to sterilize Scutellariae Radix selected based on high frequency of circulation between merchants and high susceptibility to microbial contamination, through comparing these methods in terms of the followings; ⅰ) the efficacy of sterilization in comparison with cost, and ⅱ)the alteration of chemical components of these herbal medicines. The results of this study will be taken advantage of establishment of the maximum limit of microorganism contaminated in herbal medicines. From the results of this study we conclude the followings: ⅰ) The sterilizing method using dry heat may be inappropriate, because it seriously changed their color and morphology which is an essential criterion to estimate a measure of quality between merchants and consumers, although it effectively got rid of contaminated microorganism and did not alter major components, baicalin; ⅱ) The sterilizing method using gamma irradiation may be appropriate, because it showed a strong sterilizing effect, and no alteration of chemical components, color and morphology; ⅲ)The sterilizing method using alcohol gas may also be appropriate, because it had a similar effects as in the case of gamma irradiation. Collectively, we suggest that appropriate sterilizing methods to guarantee the microbial quality of this herbal medicine may be those using gamma irradiation and alcohol gas.

Development of a Washing, Sterilization, Dehydrating System for Leaf Vegetables (엽채류 세척, 살균, 탈수 시스템 개발)

  • Hong, Seong-Gi;Park, Hoe-Man;Cho, Kwang-Hwan;Chang, Dong-Il
    • Journal of Biosystems Engineering
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    • v.32 no.6
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    • pp.408-415
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    • 2007
  • Leaf vegetables are composed of about 80% of moisture and 20 to 30% are damaged during the transportation due to decomposition and browning. This study was conducted to develop a washing, sterilization dehydrating system that could remove these polluters, and major findings were summarized as followings. The system was constructed so that leaf vegetables were moved through the washing, sterilization, and dehydration stages contained in a box, the shifting was conducted using PLC-controlled pneumatic pressure. Basic tests for determination of design parameters led to the results that optimum washing times by air bubbles were 5 minutes for lettuce and perilla and 10 minutes for Chinese cabbage, and percentages of bacteria removed using the optimum washing times were 94, 98, and 76%, respectively. Optimum speed and time of a centrifugal dehydrator for removal surface waterdrop on the leaves were 400 rpm and 30 s, respectively, dehydration rates were in a range $70{\sim}82%$ and damage rates were within 15% for the three vegetables tested. Operating efficiencies of the developed system for lettuce, perilla, and Chinese cabbage were 100, 120, and 80 kg/h, respectively, which were 25, 24, and 27 times of operating efficiencies of the conventional manual method.

Study on mold sterilization using High Electric field generation system

  • Kee-Yeon Joe
    • Proceedings of the KIPE Conference
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    • 2000.07a
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    • pp.535-538
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    • 2000
  • There are several electricity applied sterilizers such as sterilizer with high frequency sterilizer with ozone sterilizer with high voltage and so on Those sterilizers feature "because there is no chemical process there is no secondly environmental pollution" At the power conversion part ZVS and ZCS methods have been used that it results in reduced switching loss miniaturized size and lightened weight, Besides the current in the device is smaller than that of existing method. Thus it is expected that the cost of sterilization process when quality of the device is measured by power consumption will be reduced.e reduced.

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Power Converter System for Sterilization processing Device (살균처리 장치용 전력변환장치)

  • 강욱중;고강훈;서기영;이현우
    • Proceedings of the IEEK Conference
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    • 2001.06e
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    • pp.237-240
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    • 2001
  • There are several electricity applied sterilizers such as sterilizer with high frequency, sterilizer with ozone, sterilizer with high voltage, and so on. Those sterilizers feature “because there is no chemical process, there is no secondly environmental pollution” At the power conversion part, AVS and ZCS methods have been used that it results in reduced switching loss, miniaturized size, and lightened weight. Besides, the current in the device is smaller than that of existing method. Thus, it is expected that the cost of sterilization process, when quality of the device is measured by power consumption, will be reduced.

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Actual Disinfection and Sterilization Control in Korean Healthcare Facilities (국내 의료기관의 소독과 멸균 관리 실태)

  • Jeong, Sun Young;Choi, Jeong Hwa;Kim, Eun Kyoung;Kim, Su Mi;Son, Hee;Cho, Nan Hyoung;Choi, Ji Youn;Park, Eun Suk;Park, Jin Hee;Lee, Ji Young;Choi, Soon Im;Woo, Jin Ha;Kim, Og Son
    • Journal of Korean Academy of Fundamentals of Nursing
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    • v.21 no.4
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    • pp.392-402
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    • 2014
  • Purpose: This study was done to investigate the status of disinfection and sterilization in healthcare facilities. Method: A survey of 193 Korean healthcare facilities was conducted from February 8 to March 7, 2013. Data were analyzed using descriptive statistics, ${\chi}^2$ test, Fisher's exact test, one-way ANOVA, Scheffe with SPSS WIN 18.0. Results: Of the healthcare facilities 93.2% had specific guidelines for disinfection/sterilization, but only 47.9% had a committee on disinfection/sterilization for decision-making, less than half (42.7%) conducted regular monitoring of actual practices, while 83.9% had established procedures for recovery in case of problems with the disinfection process and 89.0% kept records and archives of disinfection practices. Cleaning process, selection of chemical disinfectants and process of disinfection and sterilization were found to be inadequate in some healthcare facilities. Perception score for adequacy of medical instruments was 8.10, environmental disinfection was 7.20, and sterilizer management was 8.45 out of a possible 10. Conclusion: Compared to larger institutions, smaller healthcare facilities had less effective disinfection and sterilization management systems, while some facilities showed inadequate practices for medical equipment and general sterilization. Better academic and state-level support is recommended for smaller facilities in order to establish a better system-wide management system.

Development of Ozone Plasma Sterilizer with Air Circulation Device (공기순환장치 적용 오존 플라즈마 살균장치 개발)

  • An, June;Chun, Young-Nam
    • Journal of Korean Society of Occupational and Environmental Hygiene
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    • v.31 no.1
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    • pp.105-110
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    • 2021
  • Objectives: The purpose of this study was to develop a new sterilization plasma device that would be proficient at sterilizing and removing pathogenic bacteria and viruses, and applicable to air purification systems. Methods: In order to understand the performance characteristics, the discharge characteristics of a packed bed DBD reactor and the ozone reduction characteristics of an ozone filter installed at the outlet of the reactor were investigated. Results: The novel packed-bed DBD reactor was proposed, and it was confirmed that the plasma discharge was uniformly and stably discharged throughout the entire layer, and sufficient ozone was generated for sterilization. The ozone filter was tested for three methods: adsorption, adsorption-decomposition, and catalytic decomposition. When the filter thickness was 30 mm, the ozone concentrations were 0.03 ppm, 0.01 ppm, and 0.21 ppm, respectively. The adsorption and adsorption-decomposition methods satisfied the EPA standard of less than 0.05ppm, but the catalytic decomposition method did not. Conclusions: It was confirmed that the adsorption-decomposition method has relatively excellent ozone filter performance and can provide the best ozone filter.

Development of Control Method for Strawberry Bacterial Angular Spot Disease (Xanthomonas fragariae) (딸기 세균모무늬병(Xanthomonas fragariae)의 방제를 위한 약제 선발)

  • Kim, Da-Ran;Gang, Geun-Hye;Cho, Hyun ji;Myung, Inn-Shik;Yoon, Hae-Suk;Kwak, Youn-Sig
    • The Korean Journal of Pesticide Science
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    • v.19 no.3
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    • pp.287-294
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    • 2015
  • In Korea, Strawberry bacterial angular leaf spot disease was first reported in 2010. The disease of strawberry caused serious problem for strawberry producers and export. To field test, we applied the bactericides in June and October as nursery stage and cultivation stage, respectively. In nursery stage with high temperature condition, Oxolinic acid (97.2%) showed significant control effect. In cultivation stage with low temperature condition is soil sterilization and soil non- sterilization the control effect. In cultivation stage in soil sterilization stage Validamycin (93.3%) showed reliable control effect against the disease. Also, soil non-sterilization stage with low control effect of Validamycin (80%) showed control effect the lower than a soil sterilization stage.

The effects of UV excited $TiO_{2}$ and ZnO coating on activated carbon for Escherichia coli (활성탄에 침착시킨 $TiO_2$와 ZnO가 자외선에 의하여 활성화되었을때 Escherichia coli의 살균효과에 미치는 영향에 관한 연구)

  • 최명신;정문호;김영규
    • Journal of environmental and Sanitary engineering
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    • v.10 no.3
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    • pp.105-114
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    • 1995
  • There has been increasing awareness on the importance of not only removal of organic materials but also sterilization of microbial cell in the drinking water purification research, so there has been many researches on that area. This study has been designed to analyze the effects of $TiO_{2}$ and ZnO coated on activated carbon on Escherichia coli. In this study, the sterilization power was analyzed by (1) variation of $TiO_{2}$ and ZnO concentration coated on activated carbon (2) variation of UV intensity. In addition, the kinetics between exposure time and sterilization velocity was viewed by the method of Chick. The results are as follows. 1. Survival ratio of E. coli decreased as time goes on in application of $TiO_{2}$, ZnO and $TiO_{2}{\cdot}ZnO$. In $TiO_{2}$ and ZnO, the effect increased upto certain concentration, but decreased there-after. In $TiO_{2}{\cdot}ZnO$, the effect of sterilization was in similar way among 3 combinations. 2. Survival ratio of E. coli decreased proportionately to an increase of light intensity in ZnO and $TiO_{2}{\cdit}ZnO$. In $TiO_{2}$, the survival ratio differed over extent of irradiation but the difference over the light intensity was not significant. 3. When Chick's law of sterilization was applied, m values of three concentrations of $TiO_{2}$ were 1.57,0.98, 1.96 respectively. M values of three concentration of ZnO were 1.10, 1.18,0. 11 respectively and those of three combination of $TiO_{2}{\cdot}ZnO$ were 1.17, 1.24, 1.74 respectively.

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A Study of Homogeneous Sterilization of Micro-sized Food Powder by Rotatable Low-Temperature Plasma System (회전형 저온 플라즈마 시스템을 이용한 분말식품의 균일한 살균 연구)

  • Kim, Myung Chan;Park, Duck Mo;Han, Jin Soo;Woo, In Bong;Kim, Dong Hoo;Jang, Seong Eun;Yoon, Chan Suk;Kim, In
    • The Korean Journal of Food And Nutrition
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    • v.31 no.2
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    • pp.301-307
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    • 2018
  • In this study, a relatively effective process is used to sterilize Escherichia coli on the surface of micro-sized calcium citrate powder using nitrogen and argon as process gases in a low-temperature vacuum plasma treatment. The purpose of this study is to confirm and to introduce the effectiveness of homogeneous surface treatment for the sterilization of fine inorganic powder by the rotatable low-temperature RF plasma system designed by ourselves. The results of the test using 3M petrifilm showed that there were no remarkable spots in the case of the surface of plasma treated powder, whereas the untreated powder showed many blue spots, which indicating that the E. coli was alive. After 5 days, in the same samples, the blue spots were seen to be larger and darker than before, while the plasma-treated powder showed no changes. The results from FE-SEM analysis showed that the E. coli was damaged and/or destroyed by reactive species generated in the plasma space, resulting in the E. coli being sterilized. Furthermore, the sterilization effects according to the selected parameters ($N_2$ and Ar; flow rate 30 and 50 sccm) adapted in this study were mutually similar, regardless of such different process parameters, and this indicates that homogeneous treatment of powder surfaces could be more effective than conventional methods. Therefore, the plasma apparatus used in this study may be a practical method to use in a powerful sterilization process in powder-type food.

Prooxidant-antioxidant balance and malondialdehyde over time in adult rats after tubal sterilization and vasectomy

  • Faramarzi, Azita;Seifi, Behjat;Sadeghipour, Hamid Reza;Shabanzadeh, Alireza;Ebrahimpoor, Mitra
    • Clinical and Experimental Reproductive Medicine
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    • v.39 no.2
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    • pp.81-86
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    • 2012
  • Objective: Sterilization (tubal sterilization and vasectomy) is a widely applied contraceptive method worldwide. Although most studies have described sterilization as a safe method, there are reports of tubal ligation (TL) and vasectomy complications. The aim of this study was to evaluate the effects of TL and vasectomy on the serum oxidative stress, specifically prooxidant-antioxidant balance (PAB) and malondialdehyde (MDA) levels, over time. Methods: Male and female rats were classified into vasectomy, sham-vasectomy, TL, and sham-TL groups, respectively. The PAB and MDA levels were measured on days 15 and 45 and months 3 and 6 after the intervention. For female rats, blood sampling was performed during the diestrous phase and estradiol and progesterone were also measured. Results: Serum PAB and MDA increased after TL (p<0.05). Vasectomy increased serum MDA remarkably after 45 days, 3 months, and 6 months (p<0.05). After vasectomy, serum PAB also increased although not significantly. Serum estradiol and progesterone decreased remarkably in the TL group compared to the sham group (p<0.05). Conclusion: Bilateral TL and vasectomy both increase the serum oxidative stress; however the imbalance after TL was very noticeable. As for the TL, the reduction of serum estrogen levels can be involved in this imbalance. Complications followed by TL or vasectomy could be due to increased levels of oxidants. Thus, prescribing antioxidants during and or after surgery may be a solution.