• Applied Microscopy
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• 제41권1호
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• pp.69-73
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• 2011

High-voltage electron microscope (HVEM) has higher resolution and penetration power than conventional transmission electron microscope that could be load thick specimen. Some researchers have taken this advantage of HVEM to explore 3-dimensional configuration of the biological structures including tissue and cells. Whole mount preparations has been employed to study some cell lines and primary culture cells. In this study, we would like to introduce useful whole mount preparation method for neuronal studies. The plastic coverslips were punched, covered by formvar membrane and coated with carbon. The neurons obtained embryonic 18 rat hippocampus were seeded on the prepared cover slip. The coverslips were fixed, dried in freeze drier and kept in a descicator until HVEM observation. We could observe detailed neuronal structures such as soma, dendrite and spine under HVEM without conventional thin section and heavy metal stain. The anaglyphic image based on stereo paired image ($-8^{\circ},+8^{\circ}$) provides three dimensional perception of the neuronal dendrites and their spines. This method could be applied to sophisticated analysis of dendritic spine under the various experimental conditions.

• 대한방사선기술학회지:방사선기술과학
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• 제40권1호
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• pp.121-126
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• 2017

의료용 디지털 영상 및 통신 표준 영상과 3차원 프린팅을 이용한 보청기 이어 쉘 제작기술을 연구하였다. 이는 기존에 없는 새로운 적용방법이며 보청기 수요자의 안전, 감염, 제작시간, 진행 단계를 줄일 수 있는 적용기술이다. 연구는 의료용 디지털 영상 및 통신 표준 영상으로 스테레오리소그래피 파일을 만들기 위한 0.5 mm, 1.0 mm, 2.0 mm의 볼륨으로 획득한 값을 3차원 프린터로 출력 전과 후의 과정에서 형상표면에 미치는 영향을 실험하였다. 출력 전에는 스테레오리소그래피 파일구조에 대해 상대적 관계를 비교하였고, 출력 후에는 이어 쉘 형상표면의 적층구조 간격을 현미경으로 확대하여 비교하였다. 스테레오리소그래피 파일구조 분석에서 0.5 mm, 1.0 mm, 2.0 mm의 순으로 삼각형 꼭지점, 5개 이상의 교차점, 최대 교차점의 개수가 많았으며 외이도 형상 자체의 굴곡도에 따라 Bending, Angle, Crest 영역 순으로 삼각형 구조가 조밀하게 분포하였다. 디지털 현미경에 의한 이어 쉘 형상표면은 2.0 mm, 1.0 mm, 0.5 mm 순으로 적층구조 간격이 두껍게 나타났다. 이와 같이 스테레오리소그래피 표면구조는 3차원 이어 쉘 형상의 굴곡도가 불규칙하고 스테레오리소그래피 파일을 만들기 위한 의료용 디지털 영상 및 통신 표준 데이터의 볼륨 값이 작을수록 교차하는 스테레오리소그래피 삼각형 구조는 조밀함을 알 수 있었다.

• 한국조명전기설비학회:학술대회논문집
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• 한국조명전기설비학회 2005년도 춘계학술대회논문집
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• pp.69-73
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• 2005

The arc dispersion and contacts characteristics were analyzed according to switching off Residual Current Protective Device(RCD) switch. Arc dispersion process was taken by high speed imaging system at a rate of 10,000 frames per second. When RCD was switched from on to off, art was observed and it took about 2.3[ms] from the generation of arc to the extinction of arc. When RCD was switched from off to on, arc was not observed. We repeated switching on and off 1000, 3000 and 6000 times. After repetition, the surface characteristics of contacts were taken by stereo microscope and Scanning Electron Microscope(SEM). From the scattering patterns analysis of arc and the analysis of deteriorated contacts, it could be applicable to the research of electric fire and arc suppression.

• 한국전기전자재료학회:학술대회논문집
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• 한국전기전자재료학회 2005년도 하계학술대회 논문집 Vol.6
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• pp.576-577
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• 2005

In this paper, the oxidization characteristics were analyzed according to the shapes of Residual Current Protective Device(RCD) contacts. RCD is an electrical safety device specially designed to immediately switch the electricity off when electric leakage is detected at a level harmful to a person using electrical equipment. The shapes of RCD contacts are a little bit different according to the models. When RCD is turned on, stationary and moving contact do not fit together. So, it can cause the increase of contact resistance. To discover the deterioration characteristics of RCD contacts by switching repetition, the contacts were analyzed by stereo microscope, Scanning Electron Microscope(SEM) and Energy Dispersive X-ray Spectrometer(EDS).

• 한국화재소방학회논문지
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• 제14권3호
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• pp.1-5
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• 2000

본 연구에서는 옥내 조명으로 널리 사용되고 있는 형광등용 안정기의 화재위험성을 분석하였다. 안정기권선을 실체현미경으로 분석한 결과 용융흔을 다수 발견할 수 있었으나, 육안으로 원인 판정은 불가하였다. $700^{\circ}C$ 이상에서 열열화된 안정기 권선은 연신구조(elongation structure)가 없어지고 구리입자의 확대된 형태만 보였다. 단락 권선의 금속현미경 분석에서 경계면을 중심으로 주상조직 및 보이드 성장의 규칙성이 확인된 것으로 보아 전기적인 단락이 층간에 일어났음이 증명되었다. SEM을 이용한 미세구조 분석에서 용융된 부분이 확인되었다. EDX를 이용한 스펙트라 분석에서 Cu의 구성요소인 CuL 및 CuK liness 뿐만 아니라 OK line이 고르게 관측되고 있다. 이것은 재결합 과정에서 산소가 반응에 참여했음을 의미한다.

• 한국어류학회지
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• 제34권3호
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• pp.153-159
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• 2022

한국 고유종 눈동자개 Pseudobagrus koreanus 후각기관과 서식처 환경, 생태적 습성의 연관성, 유연종들과의 특징 비교를 위해 실체현미경, 광학현미경, 주사전자현미경하에서 형태 및 조직학적 실험을 진행하였다. 후각기관의 외부 형태는 주둥이 끝에 위치한 튜브형의 전비공과 비수염(nasal barbel) 뒤쪽 기부에 위치한 틈새형(slit)의 후비공으로 구성된다. 내부의 비강에는 비선(raphe)을 중심으로 방사형 또는 서로 평행하게 배열된 많은 수의 후판으로 구성된 로제트 구조를 나타낸다. 후판에는 후감각뉴런, 지지세포, 기저세포와 림프구, 형질세포로 구성된 감각상피와 층상상피세포, 점액세포, 림프구로 구성된 비감각상피가 확인되었다. 그중 1) 41~43 (n=20)의 후판, 2) 림프구, 형질세포, 3) 중성 다당류 점액을 보유한 점액세포는 다소 정체된 유속의 서식처 환경, 후각 의존적인 야생성의 생태적 습성, 다른 유연 메기목 어류들과 비교되는 분류학적 특징으로 사료된다.

• 한국기계가공학회지
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• 제6권2호
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• pp.22-27
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• 2007

In this study, the two methods of stiffness measurement(Spring constant) of cantilever were proposed for quantitative measurement in Atomic Force Microscopy(AFM). As the 1st method for the measurement of stiffness, the probe method, which is used in the measurement of the semiconductor mechanical and electrical properties, was applied to the measurement of the cantilever. Experiments by the probe method were performed finding the resistance value of cantilever. As the results, the resistance was measured differently along with the dimension and the thickness of cantilever that determined the stiffness(spring constant) of the lever. As the 2nd method, the vibration characteristics(Dunkerley expression) is used to obtain the stiffness of the complex structure which is combined by AFM cantilever and the standard cantilever. We measured the resonant frequency from the complex structure using the micro stages and stereo microscope. As the results, we confirmed that the vibration characteristics(Dunkerley expression) is effected the micro complex structure of AFM cantilever.

• 한국식품위생안전성학회지
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• 제11권3호
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• pp.215-220
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• 1996

Korean and Chinese sesames were subjected to microscopic observation and instrumental determination of fatty acid composition and sesamolin/sesamin ratio to obtain basic data for discriminating each other. The overall appearance of both samples was differently observed by stereo microscope (X8). Fatty acid composition of sesame oils, extracted from both samples with different roasting degrees, showed a similar pattern although Chinese samples cointained about 6% higher content of stearic acid and 47% lower content of linolenic acid then Korean ones. The sesamolin/sesamin ratio was remarkably lower(.039) in Chinese samples than Korean (0.67∼0.72). showing a variation depending on producing districts. Roasting degrees of raw sesames little influenced their composition of fatty acid and sesamolin/sesamin ratio. Based on the above results, it is considered that the comparison between domestic and Chinese sesames in view of their stearic and linolenic acid contents and sesamolin/sesamin ratio might be one of the potential criteria in discriminating their production origins.

• 한국수정란이식학회지
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• 제8권2호
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• pp.143-149
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• 1993

The objective of this study was to produce calves derived from in vitro fertilization of in vitro matured follicular oocytes. Oocytes aspirated from small antral folicles of ovaries obtained at a local slaughter house were matured and fertilized in vitro. At l8hrs after insemination with Korean native cattle semen, oocytes were co-cultured for 6~7 days by utilizing co-culture system with bovine oviduct epithelial cell. After co-culture, good or excellent quality late morulae or early blastocysts were selected by morphological criteria under stereo microscope. Selected embryos were transferred to recipients on day 6 or 7 (estrus = day 0). Recipients were monitored by observation for estrus and rectal palpation after 60 days from embryo transfer. One of them went to term with the birth of a calf. This case is the first production of calf derived from in vitro fertilization in Korea.

• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 2003년도 춘계학술대회 논문집
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• pp.241-245
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• 2003

In this paper, a microassembly system based on hybrid manipulation schemes is proposed and applied to the assembly of a photonic component. In order to achieve both high precision and dexterity in microassembly, we propose a hybrid microassembly system with sensory feedbacks of vision and force. This system consists of the distributed 6-DOF micromanipulation units, the stereo microscope, and haptic interface for the force feedback-based microassembly. A hybrid assembly method, which combines the vision-based microassembly and the scaled teleoperated microassembly with force feedback, is proposed. The feasibility of the proposed method is investigated via experimental studies for assembling micro opto-electrical components. Experimental results show that the hybrid microassembly system is feasible for applications to the assembly of photonic components in the commercial market with better flexibility and efficiency.