• Molecules and Cells
• /
• v.42 no.9
• /
• pp.646-660
• /
• 2019

Abscisic acid (ABA) is a phytohormone essential for seed development and seedling growth under unfavorable environmental conditions. The signaling pathway leading to ABA response has been established, but relatively little is known about the functional regulation of the constituent signaling components. Here, we present several lines of evidence that Arabidopsis Raf-like kinase Raf10 modulates the core ABA signaling downstream of signal perception step. In particular, Raf10 phosphorylates subclass III SnRK2s (SnRK2.2, SnRK2.3, and SnRK2.6), which are key positive regulators, and our study focused on SnRK2.3 indicates that Raf10 enhances its kinase activity and may facilitate its release from negative regulators. Raf10 also phosphorylates transcription factors (ABI5, ABF2, and ABI3) critical for ABA-regulted gene expression. Furthermore, Raf10 was found to be essential for the in vivo functions of SnRK2s and ABI5. Collectively, our data demonstrate that Raf10 is a novel regulatory component of core ABA signaling.

• Journal of Convergence for Information Technology
• /
• v.9 no.9
• /
• pp.1-12
• /
• 2019

A dialog system becomes a new way of communication between human and computer. The dialog system takes human voice as an input, and gives a proper response in voice or perform an action. Although there are several well-known products of dialog system (e.g., Amazon Echo, Naver Wave), they commonly suffer from a problem of out-of-domain utterances. If it poorly detects out-of-domain utterances, then it will significantly harm the user satisfactory. There have been some studies aimed at solving this problem, but it is still necessary to study about this intensively. In this paper, we give an overview of the previous studies of out-of-domain detection in terms of three point of view: dataset, feature, and method. As there were relatively smaller studies of this topic due to the lack of datasets, we believe that the most important next research step is to construct and share a large dataset for dialog system, and thereafter try state-of-the-art techniques upon the dataset.

• Stress
• /
• v.25 no.2
• /
• pp.105-119
• /
• 2017

Backgound: The first step in disaster response is preparation, and education and training of workforce are considered as important elements of preparedness. The purpose of this study was to identify the needs of volunteers by exploring service experiences after a disaster to develop volunteer education programs. Methods: Participants were 11 volunteers who had supported the victims' families during about 1 year after the Sewol ferry disaster. Focus group interviews were conducted to investigate the experience of volunteer at the levels of individual, within-team and between-teams, and to discover the need of further training. Results: The results showed that the needs for self- and team-care and advanced education were reported. Conclusions: These results were discussed in terms of development of competence and mental health needs disaster volunteers. Limitations of this study and directions of future research were suggested.

• Journal of the Korea Institute of Information Security & Cryptology
• /
• v.20 no.1
• /
• pp.113-121
• /
• 2010

The system specification is a system theory based formal representation method for systems' structure and behavior modeling. When we make use of the system specification method in each step of software development, we can derive a hierarchical and modularized system design which enables us to manage the software development process flexibly. This research presents system specification based design of a mobile alarm system which sends alerts about illegal usage of private information and manages the response against the each alert. In our design of mobile alarm system, there are formal definition of alert message overcoming the functional limitation of mobile device and hierarchical modularized modeling of alarm processing using system specification. The efficiency of making use of the system specification is shown by applying the specification method to implementation of mobile alarm system. The contribution of this work is in design and implementation of mobile alarm system which enables us to handle the private information leakage situation more flexible way using system specification based software designing method.

• Journal of Applied Biological Chemistry
• /
• v.64 no.2
• /
• pp.141-151
• /
• 2021

Insects affect crop harvest yield and quality, making plant response mechanisms to insect herbivores a heavily studied topic. However, analysis of plant responses to honeybees is rare. In this study, comprehensive metabolic profiling of Arabidopsis thaliana exposed to honeybees was performed to investigate which metabolites were changed by the insect. A total of 85 metabolites-including chlorophylls, carotenoids, glucosinolates, policosanols, tocopherols, phytosterols, β-amyrin, amino acids, organic acids, sugars, and starch-were identified using high performance liquid chromatography, gas chromatography-mass spectrometry, and gas chromatography-time-of-flight mass spectrometry. The metabolite profiling analysis of Arabidopsis exposed to honeybees showed higher levels of stress-related metabolites. The levels of glucosinolates (glucoraphanin, 4-methoxyglucobrassicin), policosanols (eicosanol, docosanol, tricosanol, tetracosanol), tocopherols (β-tocopherol, γ-tocopherol), putrescine, lysine, and sugars (arabinose, fructose, glucose, mannitol, mannose, raffinose) in Arabidopsis exposed to honeybees were higher than those in unexposed Arabidopsis. Glucosinolates act as defensive compounds against herbivores; policosanols are components of plant waxes; tocopherols act as an antioxidant; and putrescine, lysine, and sugars contribute to stress regulation. Our results suggest that Arabidopsis perceives honeybees as a stress and changes its metabolites to overcome the stress. This is the first step to determining how Arabidopsis reacts to exposure to honeybees.

• Physical Therapy Korea
• /
• v.28 no.1
• /
• pp.77-83
• /
• 2021

Background: Although symmetry of spatio-temporal parameter and center of pressure (COP) shift during walking is associated with knee adduction moment, research on clinical association with knee osteoarthritis (OA)-related knee pain and functional scores is lacking. Objects: The aims were 1) to compare symmetry of gait parameters and COP-shift in patients with unilateral knee OA and pain and matched controls, and 2) to investigate the relationship between symmetry of gait parameters and COP-shift, and clinical measures. Methods: Female subjects (n = 16) had with unilateral radiological knee OA and pain. Healthy controls (n = 15) were age-matched to OA group. Symmetry of foot rotation, step length, stance and swing phase, lateral symmetry of COP and anterior/posterior symmetry of COP during walking was assessed. To assess the clinical variables, pain intensity, pain duration and function using Knee Osteoarthritis Outcome Survey (KOOS) subscales were collected. We compared symmetry between groups using Mann-Whitney U-test or independent t-test. Relationships between clinical measures and symmetry index measured using Spearman's correlation test. Statistical significance was set at α = 0.05. Results: Knee OA group showed significantly greater values of only lateral symmetry of COP (p < 0.01) than healthy group. Values of lateral symmetry of COP had moderate or strong correlation significantly with the intensity of knee pain, pain duration, and scores of all KOOS subscales (p < 0.01). Conclusion: Patients with unilateral knee OA and pain showed more asymmetry of lateral COP-shift during walking compared with matched healthy controls. In addition, larger asymmetry of lateral COP-shift has the moderate or strong association with worse of knee pain, worse in KOOS scores and longer duration of knee pain. Asymmetry of lateral COP-shift during walking may be one of the characteristics of unilateral knee OA as the compensatory strategy response to unilateral OA of the knee.

• Journal of the Korean Society of Industry Convergence
• /
• v.25 no.1
• /
• pp.121-129
• /
• 2022

With the recent increase in international trade volume the trade volume through ships is also continuously increasing. The treatment of ballast water goes through the following five steps, samples are taken and analyzed at each step, and samples are obtained using a suction pump. These suction pumps have low efficiency and thus need to be improved. In this study, it is to optimize the form of the impeller which affects directly improvements of performance to determine the capacity of suction pump and to fulfill the purpose of this research. To do it, we have carried out parametric design as an input variable, geometric form for the impeller. By conducting the flow analysis for the optimum form, it has confirmed the value of improved results and achieved the purpose to study in this paper. It has selected the necessary parameter for optimizing the form of the pump impeller and analyzed the property using experiment design. And it can reduce the factor of parameter for local optimization from findings to analyze the property of form parameter. To perform MOGA(Multi-Objective Genetic Algorithm) it has generated response surface using parameters for local optimization and conducts the optimization using multi-objective genetic algorithm. with created experiment cases, it has performed the computational fluid dynamics with model applying the optimized impeller form and checked that the capacity of the pump was improved. It could verify the validity concerning the improvement of pump efficiency, via optimization of pump impeller form which is suggested in this study.

• Development and Reproduction
• /
• v.26 no.2
• /
• pp.71-77
• /
• 2022

In response to luteinizing hormone (LH), a higher concentration of progesterone (P4) is produced in luteal cells of corpus luteum (CL). Mitochondria are an essential cellular organelle in steroidogenesis. The specific engagement of the concept regarding mitochondrial shaping with early stages of steroidogenesis was suggested in reproductive endocrine cells. Although the specific involvement of GTPase dynamin-related protein 1 (Drp1) with steroidogenesis has been demonstrated in luteal cells of bovine CL in vitro, its actual relationship with ovarian steroidogenesis during the estrous cycle remains unknown. In this study, while Fis1 and Opa1 protein levels did not show significant changes during the estrous cycle, Drp1, Mfn1, and Mfn2 proteins exhibited relatively lower levels at proestrus than at estrus or diestrus. 3β-HSD showed higher levels at proestrus than at estrus or diestrus. In addition, Drp1 phosphorylation (s637) was higher in proestrus than in estrus or diestrus. Immune-positive cells for Drp1, pDrp1 (s637), and 3β-HSD were all localized in the cytoplasm of luteal cells in the CL. The immune-positive cells for 3β-HSD were more frequently seen in the CL at proestrus than at estrus or diestrus. Immunoreactivity for Drp1 in luteal cells at proestrus was weaker than that at estrus or diestrus. However, pDrp1 (s637) immune-positive cells were mostly detected in luteal cells at proestrus. These results imply that steroidogenesis (P4 production) in the CL is closely related to phosphorylation of Drp1 at serine 637. Taken together, this study presents evidence that Drp1 phosphorylation at serine 637 is an important step in steroidogenesis in the CL.

• Journal of Audiology & Otology
• /
• v.24 no.2
• /
• pp.85-90
• /
• 2020

Background and Objectives: The present study aimed to compare thresholds of direct bone-conduction (BC direct) with those of behaviorally measured BC pure-tone audiometry (PTA) and objectively measured BC auditory brainstem response (ABR) to confirm the clinical feasibility of their relationships. Subjects and Methods: Young adults with normal hearing participated in the study to determine the thresholds from three measurements at four testing frequencies. In the BC direct, the vibrator of a bone-anchored hearing aid softband was placed on the right mastoid of each subject. In both PTA and ABR, a B71 bone oscillator was placed on the subject's right mastoid. While the subject's thresholds of BC direct and BC PTA were determined with a clinically routine 5-dB step procedure, BC ABR was conducted to determine the individual's hearing sensitivity by a peak V of the waveform using tone-burst and click stimuli. Results: The BC direct showed a different pattern between low and high frequencies. Precisely, its thresholds were 13.25 and 12.25 dB HL at 0.5 and 1 kHz, respectively, but 19 and 19.75 dB HL at 2 and 4 kHz, respectively. A significant positive correlation existed between BC direct and PTA at 1 kHz, which was also correlated with ABR. Conclusions: Based on the current data, the thresholds of BC direct were similar to BC PTA at low frequencies and BC ABR at high frequencies. The thresholds of BC direct might be predictable at approximately 5 dB higher (or lower) than that in PTA, although a large data set is required for standardization.

• Korean Journal of Audiology
• /
• v.24 no.2
• /
• pp.85-90
• /
• 2020

Background and Objectives: The present study aimed to compare thresholds of direct bone-conduction (BC direct) with those of behaviorally measured BC pure-tone audiometry (PTA) and objectively measured BC auditory brainstem response (ABR) to confirm the clinical feasibility of their relationships. Subjects and Methods: Young adults with normal hearing participated in the study to determine the thresholds from three measurements at four testing frequencies. In the BC direct, the vibrator of a bone-anchored hearing aid softband was placed on the right mastoid of each subject. In both PTA and ABR, a B71 bone oscillator was placed on the subject's right mastoid. While the subject's thresholds of BC direct and BC PTA were determined with a clinically routine 5-dB step procedure, BC ABR was conducted to determine the individual's hearing sensitivity by a peak V of the waveform using tone-burst and click stimuli. Results: The BC direct showed a different pattern between low and high frequencies. Precisely, its thresholds were 13.25 and 12.25 dB HL at 0.5 and 1 kHz, respectively, but 19 and 19.75 dB HL at 2 and 4 kHz, respectively. A significant positive correlation existed between BC direct and PTA at 1 kHz, which was also correlated with ABR. Conclusions: Based on the current data, the thresholds of BC direct were similar to BC PTA at low frequencies and BC ABR at high frequencies. The thresholds of BC direct might be predictable at approximately 5 dB higher (or lower) than that in PTA, although a large data set is required for standardization.