• Title/Summary/Keyword: stem node culture

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Effects of Medium Compositions and Plant Growth Regulators on in vitro Organogenesis in Cultured Explants of Platycodon grandiflorum Species

  • Kwon, Soo Jeong;Roy, Swapan Kumar;Kim, Hye-Rim;Moon, Young-Ja;Yoon, Ki-Hong;Woo, Sun Hee;Boo, Hee Ock;Koo, Jin-Woog;Kim, Hag Hyun
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.62 no.3
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    • pp.259-274
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    • 2017
  • Platycodon grandiflorum (Bell flower) is an important plant that has traditionally been used as herbal medicine for the treatment of cough, phlegm, sore throats, lung abscesses, chest pains, dysuria, and dysentery. The present study was initiated to investigate the feasibility of inducing shoot and root organogenesis in cultured explants of P. grandiflorum in a range of culture media and through use of various plant growth regulators (PGRs). The plantlets (Stem containing one node) were isolated and cultured on different concentrations of Murashige and Skoog (MS) medium supplemented with PGRs. We found that proliferation and elongation of shoots and roots could be achieved on 1/4 MS for P. grandiflorum with wild and green petals and on 1/8 MS for P. grandiflorum with double petals. The highest levels of development and elongation of adventitious shoots and roots were observed when petal explants were cultured on 1/4 MS (pH 3.8) supplemented with 5% sucrose. Increasing the agar concentration reduced shoot growth and rooting potential; nevertheless, the highest number of shoots and roots was observed on 0.6% agar. In the case of growth regulators, 1/4 MS supplemented with $1mg\;L^{-1}$ 6-benzylaminopurine (BA) was found to be best for shooting, although higher concentrations of BA tended to reduce shoot and root elongation. The highest number of shoots was achieved on $0.5mg{\cdot}L^{-1}$ thidiazuron (TDZ) from double petal explants grown on 1/8 MS. However, root and shoot elongation were found to decrease when TDZ concentrations were increased. Low concentrations of kinetin, naphthalene acetic acid, indole acetic acid, and 3-indole butyric acid induced shoot and root proliferation and elongation. Taken together, our study showed that low concentrations of PGRs induced the greatest root formation and elongation, showing that the optimal concentration of PGRs for shoot proliferation was species-dependent.

The Effect of Mixed Cultivation of Rice Varieties on the Plant Characteristics and the Occurrence of Blast under Various Fertilizer Levels (혼식(混植)한 몇가지 수도품종(水稻品種)의 생육(生育)과 병해발생(病害發生))

  • Lee, Kwang Soo;Ahn, Su Bong
    • Korean Journal of Agricultural Science
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    • v.14 no.2
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    • pp.232-247
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    • 1987
  • Three rice varietis, Dongjinbyeo, Daecheongbyeo and Chucheongbyeo, which are leading cultivars in the Chungnam area, were planted to determine their effects of mixture on the plant characteristics and the blast occurrence. The summary obtained is as follows; 1. The heading dates of each cultivars were not affected by the mixing culture. Only a day was delayed in heading date of Chucheongbyeo under commonly standard fertilizer level. 2. The effects of cultivar mixture on the plant height was high when low level of fertilizer were applied. Of the cultivar mixture, the mixing of Dongjinbyeo and Daecheongbyeo shoed 5.1% of plant height increase. The effects of mixture on the tillering number were apparent only under the standard fertilizer level. The mixture of Dongjinbyeo and Chucheongbyeo was effective in increasing the tillering number up to 5.7% over mean tillering number of each cultivars. 3. The stem height tended to increase as the fertilizer level increased and the effects of cultivar mixture on the stem height was high. The stem height of mixture of Dongjinbyeo and Daecheongbyeo was highest compared with other mixture cropping. On the contrary, the panicle length was shortened as the fertilizer level decreased and cultivars were mixed. Among the cultivar mixtures, the mixture of Dongjinbyeo and Daecheongbyeo showed the most effectives of increasing the panicle length. 4. The total dry weight of mixed and pure cultivars were compared. The mixture of two cultivars was effective in increasing the dry weight of rice upto 3.5% over the average of dry weight of each cultivars. When three cultivars were mixed, there were 8.5% of dry weight increase over not mixed cultivars. The effects of mixture on the dry weight were more apparent under standard fertilizer level. 5. The rates of Neck and Node and Branch Blast occurrence were reduced when cultivars were mixed, and their reduction rates were 11.7% and 14.0%, respectively. The occurrence of Blasts was also decreased under the lowest fertilizer level than the standard fertilizer level. The least Blast occurrence was obtained when Dongjinbyeo and Daecheongbyeo were mixed cropped. 6. Significantly high number of spikelets of per square meter was observed when Chucheongbyeo and Dongjinbyeo were mixed. However, the spikelets of panicle were the lowest when Chucheongbyeo and Dongjinbyeo were mixed. The number of panicles per square meter was increased as the fertilizer levels increased, while the number of spikelets per panicle increased as the fertilizer level decreased. 7. The effects of mixture on the maturing were apparent regardless of the fertilizer level. The 1000 grain weight was higher when lower level of fertilizer was applied. The effects of cultivar mixture on the 1000 grain weight were more apparent in the plots of Chucheongbyeo and Dongjinbyeo mixture and three cultivar mixture. 8. The grain yield increased when cultivars mixed. The increment under mixing cropping was 4.6% over mono cropping. The effects of cultivar mixture on the yield increase were more apparent under lower levels of fertilizer application. The highest yield increase was obtained when Chucheongbyeo and Daecheongbyeo were mixed, and the rate of yield increase was 6.8% over mono-cropping. 9. The grain yield was highly correlated with number of panicles per square meter and dry weight. There was not any significant relationships found among grain yield, spikelets of panicle and ripening percentage.

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Effect of Drainage Reusing Ratio on Growth and Yield of Summer-cultivated Paprika in Recycling Hydroponic Cultivation (순환식 수경재배에서 배액 재사용율이 여름작형 파프리카의 생육 및 수량에 미치는 영향)

  • Jang, Dong-cheol;Choi, Ki-Young;Kim, II-Seop
    • Journal of Bio-Environment Control
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    • v.26 no.1
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    • pp.7-12
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    • 2017
  • This experiment was conducted to analyze the effect of drainage reuse rate on the growth and fruiting of summer paprika in closed hydroponic cultivation. The experiment was carried out for 25 weeks from March to September 2015 with 0, 20, 30, 50% mixing ratio of waste nutrient solution using non - recycling hydroponic cultivation as a control. As a result, stem diameter of the test was different in the groups 1 and 2, but no difference showed as the group progressed more than 3 groups. L.A.I tended to decrease with increasing drainage mixing ratio. The number of nodes in the 50% reuse test group was 1.4 compared to the control group, but there was no significant difference. The number of harvested nodes was significantly different in the control group (11.1 nodes) and the 50% reuse test group (8.7 nodes), and the harvested nodes tended to decrease as the drainage was reused. The ratio of harvest was also the same as that of the harvesting node, and the control was the highest at 33.2% and the lowest at the 50% reuse test at 27.6%. Relative yields were reduced by 30%, 35% and 45% in the control group in the first group, and this tendency was also observed in the second and fourth groups. However, in the 3 and 5 groups, the production of 50% test group increased by 13% and 5%. The ratio of unmarketable fruit was increased 2%, 4%, 4%, and 7% in 0%, 20%, 30% and 50% reuse test, respectively. In conclusion, if the decrease in yield due to the decline in early growth is carefully managed, even if the imbalance of inorganic ions occurs after the mid-term growth, the growth of the crop will enter into a stable period and the re-use will not be worried about the growth and the yield decrease.