• Journal of Biosystems Engineering
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• 제9권1호
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• pp.11-21
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• 1984

This study was intended to analyze the dynamic force system which induced the shattering of paddy grains. A model to predict the shattering of paddy grains was developed, and physical quantities, such as mass distribution and rigidity of rice plant, needed for evaluating the minimum shattering forces were also measured. Under the assumption that rice plant right before harvesting is a vibratory system, the mathematical model of the vibratory system was developed and solved with the varied conditions of forcing functions. The results of the study were summarized as follows: 1. The shattering of grain occurred at the abscission layer of grain by the bending moments resulted from the impact force due to the collision of panicles of rice plant. 2. The vibratory model developed for milyang 23 rice variety was analyzed to give the natural frequencies of 7-9 Hz, which were closely related with the excitation frequencies of 4-10 Hz caused by various machine parts besides engine. Thus, avoiding the resonance should be taken into consideration in the design of the harvesting machinery. 3. It was analyzed to predict the lowest frequency that could develop the shattering when the excitation force was applied to the lower end of stem. The lowest frequency for the Milyang 23 rice variety ranged from 8.33 Hz to 11.66 Hz as the amplitude varied from 1 cm to 2.5 cm. 4. The degree of shattering depended upon the magnitude of the impact force and its application point. For Milyang 23 rice variety, the minimum impact force developing the shattering was $5g_f$ when it was applied at 1 cm above the lower end of stern and $1g_f$ when applied at 5 cm above the lower end of stem. 5. The minimum colliding velocity of the panicle, when it was on the ground that would just develop the shattering, was given as follows, $$V=\sqrt{\frac{K_t}{m_g}{\cdot}{{\phi}^2}}$$ where V : The colliding velocity of the panicle against ground to cause the shatteering of rice grain. (cm/sec) $K_t$ : The minimum spring constant for bending at the abscission layer of grain. (dyne-cm/rad) ${\phi}$ : The minimum shattering angle of grain (rad) $m_g$ : The maximum mass of grain. (g).

• BMB Reports
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• 제56권3호
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• pp.178-183
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• 2023

Huntington's disease (HD) is a neurodegenerative disorder, of which pathogenesis is caused by a polyglutamine expansion in the amino-terminus of huntingtin gene that resulted in the aggregation of mutant HTT proteins. HD is characterized by progressive motor dysfunction, cognitive impairment and neuropsychiatric disturbances. Histone deacetylase 6 (HDAC6), a microtubule-associated deacetylase, has been shown to induce transport- and release-defect phenotypes in HD models, whilst treatment with HDAC6 inhibitors ameliorates the phenotypic effects of HD by increasing the levels of α-tubulin acetylation, as well as decreasing the accumulation of mutant huntingtin (mHTT) aggregates, suggesting HDAC6 inhibitor as a HD therapeutics. In this study, we employed in vitro neural stem cell (NSC) model and in vivo YAC128 transgenic (TG) mouse model of HD to test the effect of a novel HDAC6 selective inhibitor, CKD-504, developed by Chong Kun Dang (CKD Pharmaceutical Corp., Korea). We found that treatment of CKD-504 increased tubulin acetylation, microtubule stabilization, axonal transport, and the decrease of mutant huntingtin protein in vitro. From in vivo study, we observed CKD-504 improved the pathology of Huntington's disease: alleviated behavioral deficits, increased axonal transport and number of neurons, restored synaptic function in corticostriatal (CS) circuit, reduced mHTT accumulation, inflammation and tau hyperphosphorylation in YAC128 TG mouse model. These novel results highlight CKD-504 as a potential therapeutic strategy in HD.

• Reproductive and Developmental Biology
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• 제35권1호
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• pp.123-129
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• 2011

The pig has been considered to serve as an appropriate model of human disease. Therefore, establishment of porcine embryonic stem cell lines is important. The purpose of the present study was to further work in this direction. We produced porcine parthenogenetic embryos, and separately aggregated two of each of two-cell ($2{\times}2$), four-cell ($2{\times}4$), and eight-cell ($2{\times}8$) embryos derived by parthenogenesis. After culture for 4 days, the developmental ability of the aggregates and total blastocyst cell numbers were evaluated. The percentage of blastocysts was significantly higher in both $2{\times}4$- and $2{\times}8$-aggregated embryos ($58.3{\pm}1.9%$ and $37.2{\pm}2.8%$, respectively) than in the control or $2{\times}2$-aggregated embryos ($23.6{\pm}1.1%$ and $12.5{\pm}2.4%$, respectively). Total blastocyst cell numbers were increased in the $2{\times}4$- and $2{\times}8$-aggregated embryos (by $44{\pm}3.0%$ and $45{\pm}3.3%$, respectively) compared with those of control or $2{\times}2$-aggregated embryos ($30.5{\pm}2.1%$ and $30.7{\pm}2.6%$, respectively; p<0.05). The levels of mRNA encoding Oct-4 were higher in both the $2{\times}4$- and $2{\times}8$-aggregated embryos than in the control. When blastocysts derived from $2{\times}4$- aggregated embryos or intact normal embryos were cultured on mouse embryonic fibroblast feeder cells to obtain porcine stem cells, blastocysts from aggregated embryos formed colonies that were better in shape compared with those derived from intact blastocysts. Together, the data show that aggregation of porcine embryos not only improves blastocyst quality but also serves as an efficient procedure by which porcine embryonic stem cells can become established.

• Journal of Korean Neurosurgical Society
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• 제65권2호
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• pp.204-214
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• 2022

Objective : Osteoporosis result from age-related decline in the number of osteoblast progenitors in the bone marrow. Probiotics have beneficial effects on the host, when administered in appropriate amounts. This study investigated the effects of probiotics expressing specific genes, especially the effects of genetically modified bone morphogenetic protein (BMP)-2-expressing Lactobacillus plantarum CJNU 3003 (LP) on ovariectomized rats. Methods : Twenty-eight female Wistar rats (250-300 g, 12 weeks old) were divided into four groups : the sham (control), the ovariectomy (OVX)-induced osteoporosis group (OVX), the OVX and LP (OVX/LP), OVX and genetically modified BMP-2-expressing LP (OVX/LP with BMP) groups. The three groups underwent bilateral OVX and two of these groups were administered two different types of LP via oral gavage daily. At 16 weeks post-OVX, blood was collected from the heart and the bilateral tibiae were extracted and were scanned by ex-vivo micro-computed tomography and stained with hematoxylin-and-eosin (H&E) and Masson's trichrome stain for pathological assessment. The serum levels of osteocalcin (OC), rat C-telopeptide of type I collagen (CTX-I), BMP-2, and receptor activator of nuclear factor-ĸB ligand (RANKL) were measured. Results : The 3D-micro-computed tomography images showed that the trabecular structure in the OVX/LP with BMP group was maintained compared with OVX and OVX/LP groups. No significant differences were detected in trabecular thickness (Tb.Th) between control and OVX/LP with BMP groups (p>0.05). Furthermore, a tendency toward increased BMD, trabecular bone volume, Tb.Th, and trabecular number and decreased trabecular separation was found in rats in the OVX/LP with BMP groups when compared with the OVX and OVX/LP groups (p>0.05). The H&E and Masson's trichrome stained sections showed a thicker trabecular bone in the OVX/LP with BMP group compared with the OVX and OVX/LP groups. There was no difference in serum levels of OC, CTX and RANKL control and OVX/LP with BMP groups (p>0.05). In contrast, significant differences were found in OC and CTX-1 levels between the OVX and OVX/LP with BMP groups (p<0.05). Conclusion : Our results showed that the expression of genetically modified BMP-2 showed inhibition effect for bone loss in a rat model of osteoporosis.

• 물과 미래
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• 제19권2호
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• pp.167-180
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• 1986

추계 수문학 분야에서 중요한 기법으로 인정이 되어져 가고 있으며 점차 이용도가 높아져 가고 있는 분해모형(Disaggregation Model)을 국내 하천유량의 모의발생에 적용가능성을 파악하기 위해서 이 모형의 구조와 매개변수 산정 방법과 년유량을 월유량으로 분해시키고 발생유량 계열의 통계학적 분석을 실시하였으며 타모형과의 비교를 위해서 Thomas-Fiering 모형을 사용하여 그 결과들을 비교 검토하여 실무에 적용시킬 수 있는 가능성을 평가하였다.

• Journal of Veterinary Science
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• 제24권6호
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• pp.83.1-83.12
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• 2023

Background: Ellipticine (Ellip.) was recently reported to have beneficial effects on the differentiation of adipose-derived stem cells into mature chondrocyte-like cells. On the other hand, no practical results have been derived from the transplantation of bone marrow stem cells (BMSCs) in a rabbit osteoarthritis (OA) model. Objectives: This study examined whether autologous BMSCs incubated with ellipticine (Ellip.+BMSCs) could regenerate articular cartilage in rabbit OA, a model similar to degenerative arthritis in human beings. Methods: A portion of rabbit articular cartilage was surgically removed, and Ellip.+BMSCs were transplanted into the lesion area. After two and four weeks of treatment, the serum levels of proinflammatory cytokines, i.e., tumor necrosis factor α (TNF-α) and prostaglandin E2 (PGE2), were analyzed, while macroscopic and micro-computed tomography (CT) evaluations were conducted to determine the intensity of cartilage degeneration. Furthermore, immuno-blotting was performed to evaluate the mitogen-activated protein kinases, PI3K/Akt, and nuclear factor-κB (NF-κB) signaling in rabbit OA models. Histological staining was used to confirm the change in the pattern of collagen and proteoglycan in the articular cartilage matrix. Results: The transplantation of Ellip.+BMSCs elicited a chondroprotective effect by reducing the inflammatory factors (TNF-α, PGE2) in a time-dependent manner. Macroscopic observations, micro-CT, and histological staining revealed articular cartilage regeneration with the downregulation of matrix-metallo proteinases (MMPs), preventing articular cartilage degradation. Furthermore, histological observations confirmed a significant boost in the production of chondrocytes, collagen, and proteoglycan compared to the control group. Western blotting data revealed the downregulation of the p38, PI3K-Akt, and NF-κB inflammatory pathways to attenuate inflammation. Conclusions: The transplantation of Ellip.+BMSCs normalized the OA condition by boosting the recovery of degenerated articular cartilage and inhibiting the catabolic signaling pathway.

• 한국산림과학회지
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• 제102권4호
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• pp.484-490
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• 2013

본 연구는 낙엽송 개체목의 가공처리 과정에서 생산되는 제재목 및 제재 부산물의 양을 분석하기 위한 시뮬레이션 모델을 개발하고자 수행되었다. 이 모델에서는 수간곡선 추정식을 이용하여 개체목의 전간재적을 산출하고 절동된 원목에 대한 목재 가공 시뮬레이션을 통해 판재 및 각재, 원주목의 규격에 따른 최대 생산가능량을 예측하였다. 또한 제재목 가공처리 과정에서 생산되는 톱밥 및 죽데기의 잔존재적에 대한 분석을 수행하였다. 그 결과 흉고직경 12 cm 이상부터 제재목의 생산이 가능한 것으로 나타났고, 제재목의 생산이 가능한 경우에는 보다 큰 규격의 판재와 각재를 생산하는 것이 상대적으로 작은 규격의 판재와 각재 혹은 원주목을 생산하는 것에 비해 더 많은 생산량을 얻을 수 있었다. 향후 이 모델은 낙엽송의 이용재적 산출을 통한 산림의 경제적 가치평가는 물론, 최근 목질계 바이오에너지 자원으로서 그 중요성이 점차 높아지고 있는 톱밥과 같은 제재 부산물의 양을 예측하는 데에도 활용될 수 있을 것이다.

• 한국통계학회:학술대회논문집
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• 한국통계학회 2002년도 춘계 학술발표회 논문집
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• pp.85-90
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• 2002

Microarray technology allows the monitoring of expression levels for thousands of genes simultaneously. In time-course experiments in which gene expression is monitored over time we are interested in testing gene expression profiles for different experimental groups. We propose a statistical test based on the ANOVA model to identify genes that have different gene expression profiles among experimental groups in time-course experiments. Using this test, we can detect genes that have different gene expression profiles among experimental groups. The proposed model is illustrated using cDNA microarrays of 3,840 genes obtained in an experiment to search for changes in gene expression profiles during neuronal differentiation of cortical stem cells.

• Environmental Sciences Bulletin of The Korean Environmental Sciences Society
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• 제4권2호
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• pp.103-110
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• 2000

In order to understand the wintertime intermittent characteristics of the trans-boundary air pollutant transport observed in East Asia, a numerical simulation of the long-range transport of pollutants was applied using an atmospheric transport model(STEM-II). The numerical simulation was carried out for the entire month of January 1997 and specific atmospheric aerosol (including sulfate, nitrate, and other ion compounds0 observation data were compared from four observation sites(Cheju Island, Kanghwa Island, Dazaifu, and Fukue Island). The observation data revealed that concentration peaks were intermittently observed at 3 to 4-day intervals plus the four observation sites exhibited a very similar spatial variation. The horizontal and spatial scale of the heavily polluted air masses was analyzed based on numerical results. The mechanism of the intermittent transport of air pollutants was clearly explained by a comparison of the observed data with the numerical output. It was found that the wind pattern variations associated with the synoptic scale pressure system changes play an extremely important role in the transport of pollutants in this region.

• 대한기계학회논문집
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• 제15권2호
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• pp.668-676
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• 1991

Turbulent flows around tube banks and in the diffuser were studied using a non-orthogonal boundary fitted coordinate system and the modified K-.epsilon. turbulence model. In these cases, many problems emerge which stem from the geometrical complexity of the flow domain and the physical complexity of turbulent flow itself. To treat the complex geometry, governing equations were reformulated in a non-orthogonal coordinate system with Cartesian velocity components and discretised by the finite volume method with a non-staggered variable arrangement. The modified K-.epsilon. model of Hanjalic and Launer was applied to solve above two cases under the condition of strong and mild pressure gradient. The results using the modified K-.epsilon. model results in both test cases.