• Asian Pacific Journal of Cancer Prevention
• /
• v.15 no.3
• /
• pp.1099-1104
• /
• 2014

Aims and Background: Ginsenoside Rh2, which exerts the potent anticancer action both in vitro and in vivo, is one of the most well characterized ginsenosides extracted from ginseng. Although its effects on cancer are significant, the underlying mechanisms remain unknown. In this study, we sought to elucidate possible links between ginsenoside Rh2 and phosphoglucose isomerase/autocrine motility factor (PGI/AMF). Methods: $KG1{\alpha}$, a leukemia cell line highly expressing PGI/AMF was assessed by western blot analysis and reverse transcription- PCR (RT-PCR) assay after transfection of a small interfering (si)-RNA to silence PGI/AMF. The effect of PGI/AMF on proliferation was measured by typan blue assay and antibody array. A cell counting kit (CCK)-8 and flow cytometry (FCM) were adopted to investigate the effects of Rh2 on PGI/AMF. The relationships between PGI/AMF and Rh2 associated with Akt, mTOR, Raptor, Rag were detected by western blot analysis. Results: KG1${\alpha}$ cells expressed PGI/AMF and its down-regulation significantly inhibited proliferation. The antibody array indicated that the probable mechanism was reduced expression of PARP, State1, SAPK/JNK and Erk1/2, while those of PRAS40 and p38 were up-regulated. Silencing of PGI/AMF enhanced the sensibility of $KG1{\alpha}$ to Rh2 by suppressing the expression of mTOR, Raptor and Akt. Conclusion: These results suggested that ginsenoside Rh2 suppressed the proliferation of $KG1{\alpha}$, the same as down-regulation of PGI/AMF. Down-regulation of PGI/AMF enhanced the pharmacological effects of ginsenoside Rh2 on KG1${\alpha}$ by reducing Akt/mTOR signaling.

• Korean Journal of Clinical Laboratory Science
• /
• v.38 no.1
• /
• pp.9-15
• /
• 2006

Peripheral bood stem cell collection (PBSCC), including peripheral blood stem cell transplantation (PBSCT), has been utilized worldwide as a very beneficial treatment method instead of allogenic Bone Marrow Transplantation (BMT) because it has many advantages such as rapid bone marrow engraftment and hematopoietic recovery, easy and safe accessibility and lower risk of rejection compared with allogenic BMT. In order to identify most the observable parameter in PBSCC, we analyzed various hematological parameters before and after PBSCC, and evaluated the correlation between the time of bone marrow engraftment and the number of CD34+ cells. Thirteen patients, who underwent 54 PBSCCs from January, 2003 to August, 2004 at Chungnam National University Hospital due to various systemic neoplasms, were analyzed in aspects of various hematological parameters including CD34+ cells using by Flow Cytometry (FCM). PBSCC harvests are described below: Mononuclear cells (MNC) $2.3{\pm}1.4{\times}10^8/kg$ and CD34+ cells $0.63{\pm}0.35{\times}10^6/kg$ on average, respectively. There was a statistical significance in Hb and Hct before and after PBSCC, but not in WBC and platelet counts. The period to reach the hematological bone marrow engraftment was 13.4(10~21) days and 19.5(11~38) days according to the criteria of absolute neutrophile counts (ANC) ${\geq}500/uL$ and platelet counts ${\geq}50,000/{\mu}L$ in peripheral blood, respectively. There was a significant correlation between the numbers of CD34+ cell and ANC (p<0.05), and a borderline significance between MNC and ANC (p=0.051). We found that a group of patients, who were infused with CD34+ cells more than $3.5{\times}10^6/kg$, reached more rapidly the period of bone marrow engraftment in platelet counts (p=0.040). This present study suggested that Hb and Hct were the most useful parameters and should be closely monitored before and after PBSCC, that a PBSCT with the dosage of more than $3.5{\times}10^6/kg$ of CD34+ cells was needed to perform successful bone marrow engraftment, and additionally that platelet counts could be more useful in indicating bone marrow engraftment than ANC.

• Proceedings of the KSME Conference
• /
• 2002.08a
• /
• pp.151-154
• /
• 2002

The twin impulse wave leads to very complicated flow fields, such as Mach stem, spherical waves, and vortex ring. The twin impulse wave discharged from the exits of the two tubes placed in parallel is investigated to understand detailed flow physics associated with the twin impulse wave, compared with those in a single impulse wave. In the current study, the merging phenomena and propagation characteristics of the impulse waves are investigated using a shock tube experiment and by numerical computations. The Harten-Yee's total variation diminishing (TVD) scheme is used to solve the unsteady, two-dimensional, compressible, Euler equations. The Mach number $M_{s}$, of incident shock wave is changed below 1.5 and the distance between two-parallel tubes, L/d, is changed from 1.2 to 4.0. In the shock tube experiment, the twin impulse waves are visualized by a Schlieren optical system for the purpose of validation of computational work. The results obtained show that on the symmetric axis between two parallel tubes, the peak pressure produced by the twin-impulse waves and its location strongly depend upon the distance between two parallel tubes, L/d and the incident shock Mach number, $M_{s}$. The predicted Schlieren images represent the measured twin-impulse wave with a good accuracy.

• Proceedings of the KSME Conference
• /
• 2003.11a
• /
• pp.568-573
• /
• 2003

When a safety valve equipped in a nuclear power plant opens in an instant by an accident, a moving shock wave propagates downstream the valve, inducing a complicated unsteady flow field. The moving shock wave may exert severe load to the structure. So, to reduce the load acting on the wall of POSRV, a gradual opening of POSRV is adopted in general. In theses connections, a numerical work is performed to investigate the effect of valve opening time on the unsteady flow fields downstream of the valve. Compressible, two-dimensional Navier-Stokes equations are used with the finite volume method. The obtained results show that sharp pressure rise through moving shock tor the case of instant opening is attenuated by employing the gradual opening of valve. It is turned that the flows for the two cases of gradual valve opening time show the similar to that of highly under-expanded one in jet structure having expansion and compression waves and Mach stem. Also, comparing with the results for the two cases of opening time, the shorter the valve opening is, the pressure gradient at the downstream of the valve becomes softly.

• 한국연소학회:학술대회논문집
• /
• 2015.12a
• /
• pp.79-81
• /
• 2015

The experimental study on gasesous laminar free-jet flow was investigated by applying high voltage alternating current (AC) to the nozzle. The jet flows were affedcted significatly by AC electric fields particularly at high voltages for applied frequencies less than 80 Hz, while those were not responded to further increased frequencies. Under certain AC conditions of applied voltgae and frequency, the laminar gaseous fuel stem was broken down at an axial distance and subsequently separtaed into some parts. The velocity fields in jet flows interactiong with applied electric fields were compared with those without having electric field. Interaction of applying electric fields with laminar free jet flow was discussed in detail, and the possible mechanism was also explained.

• Proceedings of the Korean Nuclear Society Conference
• /
• 1996.05c
• /
• pp.359-364
• /
• 1996

'93.4월에 고리원자력 4호기 운전중(원자로 출력 100%, 발전기 출력 975MWe) 주증기 차단밸브 (MSIV)의 닫힘으로 인해 발전소가 정지되었다. 밸브분해 점검결과 밸브스템이 Back Seat Ring 조립부위 Notch 부위에서 Steam Flow와 평행한 방향으로 절단되어, 밸브스템의 손상원인을 규명하기 위한 본 연구에서는 피로해석절차도에 따라 S-N 곡선에 근거하여 피로해석을 수행하였다. 피로해석결과 밸브스템의 초기균열 생성원인은 Stem Notch 부위의 제작결함과 발전소 정지시 밸브를 급속히 닫을 때 작용하는 충격하중등에 의해 발생된 것으로 추정되며 인장평균응력과 관내 유체의 진동하중의 변동응력이 조합하여 피로균열을 가속시켜 파손을 일으킨 것으로 사교된다.

• Proceedings of the Korean Society for Bio-Environment Control Conference
• /
• 1999.11a
• /
• pp.63-66
• /
• 1999

최근 식물체내의 수분상태나 흡수되는 물의 량을 측정 또는 제어하기 위하여 레이저 경류계측센서나 열수지경류센서 등이 이용되고 있으며, 특히 열수지경류센서는 줄기 속을 흐르는 물의 량을 직접 측정하므로 생육단계에 따른 수분흡수량은 물론 하루중의 시간대별로 수분소비량을 측정하는 것이 가능하다. 이러한 식물의 생체정보를 센싱하는 것은 식물체에 적합한 환경을 조성하기 위하여 매우 중요한 것이다. (중략)

• Proceedings of the SAREK Conference
• /
• 2008.11a
• /
• pp.357-362
• /
• 2008

This study have goal with reverse engineering for petrochemistry of high pressure ball valve for localization. Ball valve for development accomplished with flow analysis based on provision of ANSI B16.34, ANSI B16.10, ANSI B16.25 In order to localize the petrochemistry high pressure control valve. Numerical simulation using CFD(Computational Fluid Dynamic) in order to predict a mass flow rate and a flow coefficient form flow dynamic point of view. The working fluid assumed the water($H_2O$). The valve inlet and outlet setup a pressure boundary condition. The outlet pressure was fixed by atmospheric pressure and calculated inlet velocity 5m/s. CFD solver used STAR-CCM+ which is commercial code. The result shows change of mass flow rate according to opening and closing angle of valve. Flow decrease observed open valve that equal percentage flow paten which is general inclination of ball valve. The structural analysis used ANSYS which is a commercial code. Stress analysis result of internal pressure in valve showed lower than yield strength. This is expect to need more detail design and verification for stem and seat structure.

• Transactions of the Korean Society of Mechanical Engineers
• /
• v.15 no.2
• /
• pp.668-676
• /
• 1991

Turbulent flows around tube banks and in the diffuser were studied using a non-orthogonal boundary fitted coordinate system and the modified K-.epsilon. turbulence model. In these cases, many problems emerge which stem from the geometrical complexity of the flow domain and the physical complexity of turbulent flow itself. To treat the complex geometry, governing equations were reformulated in a non-orthogonal coordinate system with Cartesian velocity components and discretised by the finite volume method with a non-staggered variable arrangement. The modified K-.epsilon. model of Hanjalic and Launer was applied to solve above two cases under the condition of strong and mild pressure gradient. The results using the modified K-.epsilon. model results in both test cases.

• 유체기계공업학회:학술대회논문집
• /
• 2004.12a
• /
• pp.166-171
• /
• 2004

The Rate-of-Loading(ROL) effect of motor operated gate valves causes a decrease in the thrust delivered at a specific torque by the actuator under dynamic flow ${\Delta}$P conditions as compared to the no-flow, static conditions. This effect needs to be studied for the quantitative evaluation of motor operated gate valve operability. This study is performed to verify the validity for the application of ROL in the evaluation of operability of motor operated gate valves for the opening stroke. The ROL is assessed on the basis of in-situ test data for the opening and closing strokes. The results show that the distribution of the ROL for the operling sake has a tendency to the negative value when DP is higher than 150psid.