• Journal of the Korean Wood Science and Technology
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• v.39 no.5
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• pp.406-419
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• 2011

This study was carried out to offer basic information on the wood anatomy of domestic yellow-poplar (Liriodendron tulipifera L.), a new plantation species selected by Korea Forest Service as one of the promising hardwood and bioenergy sources of the future, through comparison of stem wood with root wood in the qualitative and quantitative features. In the qualitative anatomical features, growth rings were distinct in stem wood but relatively less distinct in root wood. And stem wood appeared to have pores in radial multiples of 2 to 5, sometimes clusters but root wood to have pores in radial multiples of 2 to 3, rarely clusters. And numbers of bars in scalariform perforation plates were somewhat numerous in vessel elements of root wood than in those of stem wood. Interestingly, on the other hand, more extraneous materials in the wood rays of tap root than in those of lateral root and stem were confirmed in the chemical composition analyses. In the quantitative anatomical features, pore densities were significantly greater but vessel elements were considerably narrower in stem wood than in root wood. Vessel elements and wood fibers of root wood were considerably longer than those of stem wood. Rays were somewhat more numerous in stem wood than in root wood, and only ray heights of stem wood were more or less greater in cell numbers but both ray heights and widths of stem wood were lower in dimension than those of root wood. The anatomical differences between stem wood and root wood were thought to be associated with different growth environments between the stem above ground and the root below ground.

• Journal of Plant Biology
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• v.28 no.2
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• pp.127-140
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• 1985

A comparative anatomy between the secondary xylem in the root and stem of Korean Betulaceae, including 5 genera and 6 species, was carried out in this study. Anatomical characteristics of the secondary xylem in the root and stem are as follows: Diameter of vessel and fiber is wider in the root than the stem, while the number of vessel and fiber per unit area is fewer in the root than the stem. The length of vessel element is longer in the stem than the root, whereas length of the fiber is longer in the root than the stem. Number of bar in the perforation plate is more in the stem than the root, and the angle of perforation plate is broader in the root than the stem. Number of ray per unit area is more in the root than the stem.

• Journal of Ginseng Research
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• v.31 no.3
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• pp.142-146
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• 2007

This experiment was conducted to find identification of ginseng root's age using the number of stem vestiges in rhizome. The number of stem vestiges in rhizome is a useful key to confirm the age of ginseng root as follow : 4-year-old root has two, 5-year-old root has three, 6-year-old root has four. The distribution of stem vestiges in rhizome each year root are as follow : 2 stem vestiges in 4-year-old root is 89.5%, 3 stem vestiges in 5-year-old root is 79.7%, 4 stem vestiges in 6-year-old root is 46.3%. However, the limiting factors of identification of ginseng root's age using the number of stem vestiges in rhizome is appearance of multi-stem per plant and appearance of destroyed stem vestige in rhizome. The ratio of appearance of multi-stem per plant and destroyed stem vestige in rhizome are increased according to root age.

• Journal of Ginseng Research
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• v.7 no.2
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• pp.133-147
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• 1983

This study was carried out to obtain the basic information for the development of new ginseng varieties. The two variants (violet-stem variant and yellow-berry variant) of Korean ginseng (Panax ginseng C.A. Meyer) and American ginseng (Panax quinquefolium L.) of one to four-year were used for this study. All of the characteristics, such as leaf length, leaf width, petiol length, number of leaves per plant, number of leaflets per plants, stem diameter, stem length, number of stems per plant, root length, primary root length, root diameter, root weight were determined and correlations among them were estimated. The results obtained were summarized as follows. 1. Leaf length, petiol length, number of leaves per plant, and number of leaflets per plant of Panax ginseng, violet-stem variant and yellow-berry variant, were larger than those of Panax quinquefolium at all of the plant ages, while leaf width was wider in Panax quinquefolium. 2. The length of stem of Panax quinquefolium was shorter than that of Panax ginseng, and the frequency of multi-stem plants at 4-year-old ginseng was larger in violet-stem variant than in Panax quinquefolium and yellow-berry variant. 3. In the characteristics of ginseng root, the primary root length of Panax ginseng, violet-stem variant and yellow-berry variant, were less than that of Panax quinquefolium, while root weight, root diameter, and umber of secondary root related to yield were larger in Panax ginseng. 4. The root weight per plant related to the yield had positive and highly significant correlations with stem diameter, leaf length, leaf length, leaf width, number of compound leaves and leaflets in Panax ginseng and Panax quinguefolium. 5. The root weight related to the wield of ginseng had been influenced to stem diameter, leaf length, and leaf width directly, and number of compound leaves and leaflets indirectly. 6. The number, total area and activity of stomate per mm2 of Panax quinquefolium were more, larger and stronger than those of Panax ginseng.

• Journal of Ginseng Research
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• v.9 no.1
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• pp.42-53
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• 1985

This study was carried out to obtain the basic information about the large scale propagation of ginseng (Panax ginseng C.A. Meyer). Therefore, the stem cuttings of 1-year old ginseng, treated with various concentrations of plant growth regulators for 5 seconds (quick dipping) and 24 hours (prolonged soaking), were cultured. The root formation of stem cuttings was varied with the concentrations, kinds, and treatment methods of plant growth regulators. Besides normal-looking roots various malformed roots were observed. In the prolonged soaking method, the culture of stem cuttings, treated with 10 ppm of IBA or NAA, resulted in profuse root regeneration. And stem cuttings, in quick dipping method, treated with 2000 ppm of IBA or NAA resulted in more excellent root regeneration. In general, IBA was more vigorous for the root formation than NAA, The treatment with 50 ppm kinetin or 100 ppm BA brought good result for the retardation of senescence of stem cuttings and BA treatment was more effective than kinetin. As for the saponin content of roots derived from stem cutting culture, the roots, formed by non-treatment of growth regulators, were higher in saponin content than those formed by treatment of growth regulators.

• Journal of the Korean Society of Food Culture
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• v.16 no.3
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• pp.235-242
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• 2001

Chemical properties of the different parts of watercress(Oenanthe javanica D.C.) grown and harvested from the culture fields under different cultivating methods were studied. In proximate analyses of watercress in parts, moisture contained more in stem than in root or in leaf, but crude protein and crude lipid contents were lower in stem than in root or in leaf. Crude ash in root contained up to about double amount in leaf or stem. Major mineral elements detected in watercress were Fe, Mg, Ca, and K, and their contents in root was higher than those in stem or leaf. Especially, Fe in root was significantly higher than that in stem or in leaf. Total free sugar composed mainly with fructose and glucose, was the highest in watercress from Hwasoon and followed the watercress from Jeonju and Donggok in order. Free sugar content was highest in leaf and the lowest in root. Major water-soluble vitamins were vitamin C, thiamin and biotin and the content of vitamin C was higher than others. These vitamins contained more in leaf than in stem or root. Niacin contained 6.09 mg/100 g in leaf of watercress from Hwasoon, which was much higher than others, but it was not detected in stem of watercress from Jeonju. Organic acids detected were oxalic acid, citric acid and malic acid and other 12 organic acids were not detected. In fatty acid composition, there were significant differences among watercresses from different parts and different culture fields. Linoleic acid, linolenic acid and palmitic acid were major fatty acids contained in watercress and it took about 80% of the total content. Amino acid content in leaf was higher than that in root and in stem. Glutamic acid and proline were major amino acids in stem of watercress from Jeonju and in stem of watercresses from Hwasoon and Donggok, respectively. In leaves of all three watercresses glutamic acid content was the highest.

• Korean Journal of Medicinal Crop Science
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• v.25 no.4
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• pp.217-223
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• 2017

Background: In Korea, 6-year-old ginseng root is economically more important than 4 or 5-year-old roots. In general, the root age is determined by counting the number of stem vestiges. However, this method does not accurately estimate ginseng root age. Methods and Results: In this study, the stem vestige counting method was used to survey a total of 18,395 fresh ginsengs cultured in 2014, and 2015, to determine the accuracy of this method. The proportion of 6-year-old roots, with more than four stem vestiges, was 46.1% in 2014. For the cultivar Chunpoong cultivated in Eumseong and Goesan countries in 2015, the proportion of more than four stem vestiges was 55.9%, and 43.5%, respectively. The proportion of more than four stem vestiges for the Gumpoong cultivated in Eumseong and Yangpyeong countries was 67.0%, and 35.1%, respectively, whereas that for the cultivar Yunpoong was 36.0% and 61.0%, respectively. Moreover, it was confirmed that differences in the levels of Rg1 will enable root age determination. Conclusions: Root age determination by the stem vestige test was found to differ depending on the environmental and cultivation conditions. To determine the age of ginseng roots, a comprehensive method, such as counting stem vestiges and evaluating differences in ginsenoside levels, should be applied.

• Natural Product Sciences
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• v.13 no.4
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• pp.273-278
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• 2007

This paper presents a detailed pharmacognostical study of the stem and root of the crude drug Ipomoea quamoclit Linn. (Convolvulaceae). Morphoanatomy of the stem and root have been studied with the aim to aid pharmacognostic and taxonomic species identification using light and confocal microscopy, WHO recommended physico-chemical determinations and authentic phytochemical procedures. The physico-chemical, morphological and histological parameters presented in this paper may be proposed as parameters to establish the authenticity of stem and root of I. quamoclit and may possibly help to differentiate the drug from its other species.

• Journal of the Korean Wood Science and Technology
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• v.42 no.2
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• pp.138-146
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• 2014

Stem and root of Lindera erythrocarpa were described and compared in the wood anatomical aspects. Root wood appeared to differ from stem wood in the qualitative features of growth ring boundary, extraneous materials in vessel element and ray parenchyma cell, outline of ray, and sheath cell. In the quantitative features, there were differences between these two tissues in vessels per square millimeter, tangential diameter of vessel lumina, length of vessel element, and width of ray. These wood anatomical differences between stem above ground and root below ground were thought to be attributed to their different growth environments.

• Journal of Ginseng Research
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• v.35 no.2
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• pp.209-218
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• 2011

Ginseng has been used as a general tonic agent to invigorate the human body as an adaptogenic agent. In a previous report, we have shown that ginseng contains a novel glycolipoprotein called gintonin. The main function of gintonin is to transiently enhance intracellular free $Ca^{2+}$ $[Ca^{2+}]_i$ levels in animal cells. The previous method for gintonin isolation included multiple steps using organic solvents. In the present report, we developed a simple method for the preparation of crude gintonin from ginseng root as well as stem and leaf, which produced a higher yield of gintonin than the previous one. The yield of gintonin was 0.20%, 0.29%, and 0.81% from ginseng root, stem, and leaf, respectively. The apparent molecular weight of gintonin isolated from stem and leaf through sodium dodecyl sulfate polyacrylamide gel electrophoresis was almost same as that from root but the compositions of amino acids, carbohydrates or lipids differed slightly between them. We also examined the effects of crude gintonin from ginseng root, stem, and leaf on endogenous $Ca^{2+}$-activated $Cl^-$ channel (CaCC) activity of Xenopus oocytes through mobilization of $[Ca^{2+}]_i$. We found that the order of potency for the activation of CaCC was ginseng root > stem > leaf. The $ED_{50}$ was $1.4{\pm}1.4$, $4.5{\pm}5.9$, and $3.9{\pm}1.1$ mg/mL for root, stem and leaf, respectively. In the present study, we demonstrated for the first time that in addition to ginseng root, ginseng stem and leaf also contain gintonin. Gintonin can be prepared from a simple method with higher yield of gintonin from ginseng root, stem, and leaf. Finally, these results demonstrate the possibility that ginseng stem and leaf could also be utilized for ginstonin preparation after a simple procedure, rather than being discarded.