• 임산에너지
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• 제17권1호
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• pp.56-70
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• 1998

This study was carried out to investigate the structural characteristics of kraft lignin and the wood degrading characteristics, the productivity of ligninolytic enzymes and the enzymatic degradation of kraft lignin by white-rot fungi. To purify kraft lignin, precipitation of kraft pulping black liquors of pitch pine meal was done by titration with lN $H_{2}SO_{4}$ reaching to pH 2, and isolation of the precipitates done by centrifugation. The isolated precipitates from pitch pine were redissloved in lN NaOH, reprecipitated by titration with lN $H_{2}SO_{4}$, washed with deionized water, and kept ofr analysis after freeze drying. Fractionation of the precipitates in solution by successive extraction with $CH_{2}Cl_{2}$ and MeOH, and the fractionates were named SwKL, SwKL I, SwKL II, and SwKL III for pitch pine kraft lignin. The more molecular weights of kraft lignin increased, the less phenolic hydroxyl groups and the more aliphatic hydroxyl groups. Because as the molecular weights increased, the ratio of etherified guaiayl/syringyl(G/S ratio) and the percentage were increased. The spectra obtained by 13C NMR and FTIR assigned by comparing the chemical shifts of various signals with shifts of signals from autherized ones reported. The optimal growth temperature and pH of white-rot fungi in medium were $28^{\circ}C$ and 4.5-5.0, respectively. Especially, in temperature and pH range, and mycelial growth, the best white-rot fungus selected was Phanerochaete chrysosporium for biodegradation. For the degradation pathways, the ligninolytic fungus jcultivated with stationary culture using medium of 1% kraft lignin as a substrate for 3 weeks at $28^{\circ}C$. The weight loss of pitch pine kraft lignin was 15.8%. The degraded products extracted successively methoanol, 90% dioxane and diethyl ether. The ether solubles were analyzed by HPLC. Kraft lignin degradation was initiated in $\beta$-O-4 bonds of lignin by the laccase from Phanerochaete chrysosporium and the degraded compounds were produced from the cleavage of $C\alpha$-$C\beta$ linkages at the side chains by oxidation process. After $C\alpha$-$C\beta$ cleavage, $C\alpha$-Carbon was oxidized and changed into aldehyde and acidic compounds such as syringic acid, syringic aldehyde and vanilline. And the other compound as quinonemethide, coumarin, was analyzed. The structural characteristics of kraft lignin were composed of guaiacyl group substituted functional OHs, methoxyl, and carbonyl at C-3, -4, and -5 and these groups were combinated with $\alpha$ aryl ether, $\beta$ aryl ether and biphenyl. Kraft lignin degradation pathways by Phanerochaete chrysosporium were initially accomplished cleavage of $C\alpha$-$C\beta$ linkages and $C\alpha$ oxidation at the propyl side chains and finally cleavage of aromatic ring and oxidation of OHs.

• 대한교통학회지
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• 제27권1호
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• pp.129-141
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• 2009

복합운송은 두 개 이상의 수송수단을 이용하는 기점에서 종점까지의 수송으로 정의될 수 있다. 복합운송이 허브 네트워크에 활용되면 집화된 수송량이 보다 적절한 수단들과 기술들에 의해 수송되기 때문에 네트워크 효율성이 제고될 수 있다. 이러한 장점에도 불구하고 문제의 복잡성 등으로 인하여 복합운송 허브 네트워크 설계 문제에 관한 연구는 그동안 활발하게 수행되지 않았다. 본 연구의 목적은 단일할당 전략을 이용하는 복합운송 허브 네트워크 설계 최적화 모형을 개발하는 것이다. 본 연구에서 개발된 모형은 수송비용, 재고비용, 서비스지체비용 등 복합운송 허브 네트워크에서 발생하는 다양한 비용요소들을 고려하는 한편, 운행빈도 변수를 사용함으로써 수송량 집화에 따른 수송 규모의 경제 효과를 내생적으로 결정할 수 있어 복합운송을 활용하는 실제 허브 네트워크의 특성들을 잘 반영할 수 있다. 개발된 모형은 비선형 정수계획 문제의 복잡한 구조를 가지고 있기 때문에, 본 연구에서는 모형에 대한 해석적 연구를 통하여 모형을 단순화함으로써 향후 알고리즘을 개발하기 위한 이론적 출발점을 제시한다. 본 연구는 복합운송 허브 네트워크의 설계뿐만 아니라 기존의 물류시스템 평가에도 기여할 수 있을 것으로 사료된다.